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排序方式: 共有106条查询结果,搜索用时 15 毫秒
11.
Hillenmeyer ME Fung E Wildenhain J Pierce SE Hoon S Lee W Proctor M St Onge RP Tyers M Koller D Altman RB Davis RW Nislow C Giaever G 《Science (New York, N.Y.)》2008,320(5874):362-365
Genetics aims to understand the relation between genotype and phenotype. However, because complete deletion of most yeast genes ( approximately 80%) has no obvious phenotypic consequence in rich medium, it is difficult to study their functions. To uncover phenotypes for this nonessential fraction of the genome, we performed 1144 chemical genomic assays on the yeast whole-genome heterozygous and homozygous deletion collections and quantified the growth fitness of each deletion strain in the presence of chemical or environmental stress conditions. We found that 97% of gene deletions exhibited a measurable growth phenotype, suggesting that nearly all genes are essential for optimal growth in at least one condition. 相似文献
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Ryan EP Heuberger AL Weir TL Barnett B Broeckling CD Prenni JE 《Journal of agricultural and food chemistry》2011,59(5):1862-1870
Emerging evidence supporting chronic disease fighting properties of rice bran has advanced the development of stabilized rice bran for human use as a functional food and dietary supplement. A global and targeted metabolomic investigation of stabilized rice bran fermented with Saccharomyces boulardii was performed in three rice varieties. Metabolites from S. boulardii-fermented rice bran were detected by gas chromatography-mass spectrometry (GC-MS) and assessed for bioactivity compared to nonfermented rice bran in normal and malignant lymphocytes. Global metabolite profiling revealed significant differences in the metabolome that led to discovery of candidate compounds modulated by S. boulardii fermentation. Fermented rice bran extracts from three rice varieties reduced growth of human B lymphomas compared to each variety's nonfermented control and revealed that fermentation differentially altered bioactive compounds. These data support that integration of global and targeted metabolite analysis can be utilized for assessing health properties of rice bran phytochemicals that are enhanced by yeast fermentation and that differ across rice varieties. 相似文献
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Matthew W. Menchaca Cody B. Scott Kirk W. Braden Corey J. Owens Loree A. Branham 《Strength and Conditioning Journal》2011,64(6):669-673
Goat browsing can be used as an alternative brush management option for redberry (Juniperus pinchotii Sudw.) and ashe (Juniperus asheii Buch) juniper instead of more expensive and invasive brush control methods, assuming consumption of juniper does not adversely affect the marketability of offspring. Some wildlife species reportedly retain juniper flavor when consumed. We determined if juniper consumption affected meat quality or flavoring of Boer-cross kid carcasses. Twenty recently weaned, Boer-cross wethers were randomly assigned to one of four treatments with treatments fed different amounts of juniper (0%, 10%, 20%, 30% juniper in the diet). All goats were fed juniper for 28 d at the Angelo State University (ASU) Management, Instruction, and Research Center. All goats were also fed a feedlot ration to meet maintenance requirements (2% body weight). Juniper intake varied (P < 0.05) between all treatments (0%, 10%, 20%, 30%) primarily because treatments were fed different amounts of juniper. Following a 28-d trial, goats were harvested at the ASU Food Safety and Product Development Laboratory. Carcass characteristics including live weight, hot carcass weight, dressing percentage, loineye area, body wall fat thickness, and leg circumference were similar (P > 0.05) among treatments. Sensory characteristics including tenderness, juiciness, flavor intensity, off-flavor, and overall acceptability were also similar (P > 0.05) among treatments. Landowners can utilize goats as a biological management tool without adversely affecting goat meat quality or flavoring. 相似文献
16.
de Little SC Williamson GJ Bowman DM Whelan PI Brook BW Bradshaw CJ 《Pest management science》2012,68(5):709-717
BACKGROUND: Microbial and insect‐growth‐regulator larvicides dominate current vector control programmes because they reduce larval abundance and are relatively environmentally benign. However, their short persistence makes them expensive, and environmental manipulation of larval habitat might be an alternative control measure. Aedes vigilax is a major vector species in northern Australia. A field experiment was implemented in Darwin, Australia, to test the hypotheses that (1) aerial microbial larvicide application effectively decreases Ae. vigilax larval presence, and therefore adult emergence, and (2) environmental manipulation is an effective alternative control measure. Generalised linear and mixed‐effects modelling and information‐theoretic comparisons were used to test these hypotheses. RESULTS: It is shown that the current aerial larvicide application campaign is effective at suppressing the emergence of Ae. vigilax, whereas vegetation removal is not as effective in this context. In addition, the results indicate that current larval sampling procedures are inadequate for quantifying larval abundance or adult emergence. CONCLUSIONS: This field‐based comparison has shown that the existing larviciding campaign is more effective than a simple environmental management strategy for mosquito control. It has also identified an important knowledge gap in the use of larval sampling to evaluate the effectiveness of vector control strategies. Copyright © 2011 Society of Chemical Industry 相似文献
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Stuart P. Hardegree Corey A. Moffet Gerald N. Flerchinger Jaepil Cho Bruce A. Roundy Thomas A. Jones Jeremy J. James Patrick E. Clark Frederick B. Pierson 《Strength and Conditioning Journal》2013,66(2):127-135
The microclimatic requirements for successful seedling establishment are much more restrictive than those required for adult plant survival. The purpose of the current study was to use hydrothermal germination models and a soil energy and water flux model to evaluate intra- and interannual variability in seedbed microclimate relative to potential germination response of six perennial grasses and cheatgrass. We used a 44-yr weather record to parameterize a seedbed microclimate model for estimation of hourly temperature and moisture at seeding depth for a sandy loam soil type at the Orchard Field Test Site in southwestern Ada County, Idaho. Hydrothermal germination response was measured in the laboratory for two seed lots of cheatgrass (Bromus tectorum L.), four seed lots of bluebunch wheatgrass (Pseudoroegneria spicata [Pursh] Löve), three seed lots of bottlebrush squirreltail (Elymus elymoides [Raf] Swezey), and one seed lot each of Sandberg bluegrass (Poa secunda J. Presl.), big squirreltail (Elymus multisetus [J.G. Smith] M.E. Jones), thickspike wheatgrass (Elymus lanceolatus [Scribn. And J.G. Smith] Gould) and Idaho fescue (Festuca idahoensis Elmer). Germination response models were developed to estimate potential germination rate for 13 subpopulations of each seed lot for every hour of the 44-yr simulation. Seedbed microclimate was assessed seasonally and for each day, month, and year, and germination rate-sum estimates integrated for a numerical index of relative site favorability for germination for each time period. The rate-sum favorability index showed a consistent pattern among seed lots for different years, and provides a relatively sensitive indicator of annual and seasonal variability in seedbed microclimate. This index could be used with field data to define minimum weather thresholds for successful establishment of alternative plant materials, in conjunction with weather forecast models for making restoration and fire-rehabilitation management decisions in the fall season, for evaluation of potential climate-change impacts on plant community trajectories, and in optimization schemes for selecting among alternative restoration/rehabilitation management scenarios. 相似文献
18.
Corey J.A. Bradshaw Ben M. Fitzpatrick Craig C. Steinberg 《Biological conservation》2008,141(7):1894-1905
Over-exploitation of whale sharks threatens the future of these wide-ranging pelagic fish. A long-term continuous record (4436 sightings) from a large aggregation (300-500 resident individuals) of whale sharks at Ningaloo Reef, Western Australia shows that mean shark length declined linearly by nearly 2.0 m and relative abundance measured from ecotourism sightings (corrected for variation in search effort and environmental stochasticity) has fallen by approximately 40% over the last decade. This population-level result confirms previous predictions of population decline based on projection models parameterised using mark-recapture estimates of survival. The majority of these changes are driven by reductions in the number of large individuals in the population. Phenomenological time series models support a deterministic (extrinsic) decline in large females, although there was some evidence for density dependence in large males. These reductions have occurred despite the total protection of whale sharks in Australian waters. As this species is highly migratory, the rapid change in population composition over a decade (<1 whale shark generation) supports the hypothesis of unsustainable mortality in other parts of their range (e.g., overfishing), rather than the alternative of long-term abiotic or biotic shifts in the environment. As such, effective conservation of whale sharks will require international protection, and collaborative tagging studies to identify and monitor migratory pathways. 相似文献
19.
Centaurea maculosa is a plant species native to Eurasia and invasive in many regions of North America that secretes a phytotoxin from its roots, (+/−)-catechin, which is thought to serve an allelopathic role by reducing fitness of neighboring species. However, catechin is a relatively unstable compound, is often found at relatively low concentrations, interacts with soil cations to form insoluble complexes and/or degradation products, and exhibits extremely variable accumulation patterns in the soil. These factors, coupled with a lack of knowledge of the regulation of catechin production by C. maculosa, confound our understanding of the importance of catechin as an allelochemical. The time and cost of current sampling procedures effectively limit the observations made on catechin accumulation patterns, without which it is extremely difficult to conclusively attribute allelopathic significance to this compound. Thus, a highly sensitive, fast, inexpensive and reliable method for soil catechin analysis is needed. We report a novel method that utilizes the colorimetric reagent dimethylaminocinnamaldehyde (DMACA) in an acidic ethanol solution for detection of soil catechin. This method is selective and extremely sensitive and can be used in the field for qualitative, but not quantitative, analysis. This assay will allow for a greater understanding of the role of catechin as an allelochemical. 相似文献
20.
Ingeborg M. Mulder Corey J. Morris J. Brian Dempson Ian A. Fleming Michael Power 《Ecology of Freshwater Fish》2018,27(3):785-797
Anadromous Arctic charr, Salvelinus alpinus, feed in the marine environment for several months during the summer and migrate back to fresh water in late summer to spawn and/or overwinter. While overwintering, anadromous Arctic charr are generally believed to reduce or cease feeding, and they are poorly described in their winter movement activity. This study used telemetry data collected from two locations to describe overwintering movement activity, including interindividual variation. Movement activity declined markedly during the ice‐covered period, suggesting opportunistic maintenance feeding was used as an energy conservation strategy. Fall and spring movement was correlated with daylight hours, and ice break‐up had a significant effect on the timing of outmigration. Movement activity was negatively correlated with body length, with smaller individuals being more active than larger fish. Although general activity patterns were evident, there were significant differences among individuals, particularly during spring immediately prior to lake departure. Lake size and individual differences in metabolic rate may account for some of this variation. 相似文献