Foal IgG and opsonizing anti-Rhodococcus equi antibodies after immunization of pregnant mares with a protective VapA candidate vaccine     

Cauchard J  Sevin C  Ballet JJ  Taouji S 《Veterinary microbiology》2004,104(1-2):73-81

The aim of this study was to evaluate serum IgG antibody levels and opsonizing activity in foals from pregnant mares immunized with either proteins from an R. equi strain containing virulence-associated protein A (VapA), an immunodominant surface-expressed lipoprotein encoded by a virulence plasmid crucial for virulence in foals, or a whole killed virulent R. equi preparation. Forty-eight pregnant mares were distributed into three groups, i.e. 24 immunized with R. equi VapA protein antigen associated with a water-based nanoparticle adjuvant (Montanide IMS 3012), 8 immunized with whole killed R. equi, and 16 non-immunized as control. Serum IgG and opsonizing capacity were evaluated during pregnancy in mares, and up to day 45 post-delivery in foals in which R. equi infections were recorded in the first 6 months of life. Pregnant mares immunized with virulent R. equi proteins developed higher serum IgG and opsonic activity which were transferred to the foals than either in the whole R. equi immunized or the control group. Four foals developed pneumonia in the control group while none in immunized groups. Results support further evaluation of VapA protein antigen associated with a water-based nanoparticle adjuvant as a candidate vaccine for immunization of pregnant mares resulting in passive antibody-mediated protection of foals.  相似文献   

Expressed sequence tags from the phytopathogenic fungus Botrytis cinerea     

Muriel Viaud  Fabrice Legeai  Jean-Marc Pradier  Yves Brygoo  Frédérique Bitton  Jean Weissenbach  Adeline Brunet-Simon  Aymeric Duclert  Sabine Fillinger  Dominique Fortini  Anastasia Gioti  Corinne Giraud  Sébastien Halary  Isabelle Lebrun  Pascal Le Pêcheur  Delphine Samson  Caroline Levis 《European journal of plant pathology / European Foundation for Plant Pathology》2005,111(2):139-146

A large set of genes was identified in the phytopathogenic fungus Botrytis cinerea by using an expressed sequence tag approach. The fungus was grown in axenic culture and a cDNA library was produced. From this library, 6559 ESTs were obtained. The combined sequences represent 3026 unisequences that corresponds to approximately one-quarter of the estimated total number of genes in B. cinerea. Approximately 18% of the ESTs showed significant similarities with genes coding for proteins with known functions,~56% were similar to genes coding for proteins with unknown functions and ~26% were orphans. A substantial B. cinerea gene inventory including putative virulence factors was therefore obtained and is now available at the Génoplante-Info Database interface (http://urgi.infobiogen.fr///Projects/GPiDB/Interface/).  相似文献   

HAEMOLYTIC DISEASE OF THE NEWBORN IN CALVES   总被引：1，自引：0，他引：1  

Corinne K. Dimmock  K. Bell 《Australian veterinary journal》1970,46(2):44-47

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Transmission of pathogenic respiratory bacteria to specific pathogen free pigs at slaughter     

Marois C  Cariolet R  Morvan H  Kobisch M 《Veterinary microbiology》2008,129(3-4):325-332

The purpose of this study was to evaluate the transmission of pathogenic respiratory bacteria to thirteen 5-month-old specific pathogen free (SPF) pigs, during the slaughtering process in a commercial slaughterhouse. Before transportation, the SPF pigs and the lorry were checked to confirm the absence of pathogenic respiratory bacteria.

Nine SPF pigs (group 1) were in contact in a conventional slaughterhouse with finishing pigs, during 4 h before slaughtering. Four SPF pigs (group 2) were slaughtered immediately at arrival in the slaughterhouse.

Five bacterial pathogens (Mycoplasma hyopneumoniae, Actinobacillus pleuropneumoniae, Pasteurella multocida, Haemophilus parasuis and Streptococcus suis) were detected by PCR, after slaughtering, from nasal cavities, tonsils and trachea in the two groups of pigs. Lung samples were PCR negative. Three and four bacterial species were isolated from the pigs of group 2 and group 1, respectively. Cultures were negative from the lungs.

All the bacterial species present in the SPF pigs were detected by PCR. P. multocida was isolated, from three samples of scalding water before the onset of slaughtering.

Our results suggest that the SPF pigs became contaminated mainly by the slaughterhouse environment and the scalding water. Histological examinations revealed that during scalding, contaminated water could reach the trachea and the lungs of pigs. Checks conducted at slaughter for respiratory disorders have to be carried on, but nasal cavities and tonsils are not appropriate for bacteriological investigations. Moreover, bacteriological results obtained from the lungs of slaughtered pigs have to be used with carefulness.  相似文献   

An evolutionary ecology perspective to address forest pathology challenges of today and tomorrow     

Marie-Laure Desprez-Loustau  Jaime Aguayo  Cyril Dutech  Katherine J. Hayden  Claude Husson  Boris Jakushkin  Benoît Marçais  Dominique Piou  Cécile Robin  Corinne Vacher 《Annals of Forest Science》2016,73(1):45-67

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Optimal management of molds in stored corn     

Yigezu A. Yigezu  Corinne E. Alexander  Paul V. Preckel  D.E. Maier  C.P. Woloshuk  L.J. Mason  J. Lawrence  D.J. Moog 《Agricultural Systems》2008

Long term storage of corn is becoming more common due to the recent increase in the demand for corn by ethanol plants. Infection of maize kernels by toxigenic fungi remains a challenging storage problem despite decades of research. Experts in storage management propose the use of a combination of preventive and monitoring-based responsive strategies in response to mold risks. In this paper, a stochastic dynamic programming model is solved to determine the expected profitability and optimal combination, timing, and intensity of the proposed mold management strategies using farmers’ existing infrastructure. The results show that even with relatively high monitoring costs, maintaining high quality grain using a monitoring-based optimal mold management strategy costs less than the benefit it fetches. The current typical practice by Indiana farmers of aerating the grain until the end of December and doing nothing thereafter bears a high risk of economic losses if grain is to be stored until later during the summer. Generally, the optimal mold management strategy depends on monitoring the biophysical conditions of the grain and the time period under consideration. If the in-bin temperature is high and less than 5% of kernels are mold damaged, then aerating when the outside temperature is at least 3 °C less than the in-bin temperature and continuing to store the grain is the optimal strategy.  相似文献   

Skeletal metastasis from a squamous cell carcinoma of the nictitating membrane in a Haflinger horse     

M. de Preux  C. Gurtner  I. Klebic  M. A. Waschk  C. Drögemüller  H. P. Brünisholz 《Equine Veterinary Education》2021,33(5):e140-e145

A 12-year-old Haflinger gelding with a history of a persistent cough was referred for evaluation of a severe lameness of the left forelimb. An excision of the right nictitans had been performed 2 years prior to presentation, and a squamous cell carcinoma (SCC) with embolic neoplastic cells in several blood vessels had been confirmed by histopathology. The origin of the lameness could not be localised with regional analgesia; therefore, a nuclear scintigraphic examination was performed. This revealed an area of marked increased radiopharmaceutical uptake at the level of the caudodorsal border of the left scapula. Further examination, including ultrasound-guided biopsy of the suspect region, confirmed the presence of SCC invading the scapula. Due to poor a prognosis, the horse was subjected to euthanasia. Prior to euthanasia, the gelding was tested homozygous for the missense variant in the damage-specific DNA-binding protein 2 (DDB2) gene, which is reported as a risk factor for the development of nictitans SCC in Haflinger horses. Post-mortem evaluation revealed multiple SCC metastasis, affecting the scapula, the liver and the lungs. To the authors' knowledge, this is the first reported case of bone metastasis following a primary periocular SCC in a horse.  相似文献   

Variation in aggressiveness is detected among Puccinia triticina isolates of the same pathotype and clonal lineage in the adult plant stage     

Bénédicte Pariaud  Henriette Goyeau  Fabien Halkett  Corinne Robert  Christian Lannou 《European journal of plant pathology / European Foundation for Plant Pathology》2012,134(4):733-743

Puccinia triticina reproduces asexually in France and thus individual genotype is the unit of selection. A strong link has been observed between genotype identities (as assessed by microsatellite markers) and pathotypes (pools of individuals with the same combination of qualitative virulence factors). Here, we tested whether differences in quantitative traits of aggressiveness could be detected within those clonal lineages by comparing isolates of identical pathotype and microsatellite profile. Pairs of isolates belonging to different pathotypes were compared for their latent period, lesion size and spore production capacity on adult plants under greenhouse conditions, with a high number of replicates. Isolates of the same pathotype showed remarkably similar values for the measured traits, except in three situations: differences were obtained within two pathotypes for latent period and within one pathotype for sporulation capacity. One of these differences was tested again and confirmed. This indicates that the average aggressiveness level of a leaf rust pathotype may increase without any change in its virulence factors or microsatellite profile.  相似文献   

Epidemiological study on Mycoplasma synoviae infection in layers     

Dufour-Gesbert F  Dheilly A  Marois C  Kempf I 《Veterinary microbiology》2006,114(1-2):148-154

Mycoplasma synoviae infection occurs worldwide in commercial poultry flocks and may result in severe economic losses. The prevalence of this mycoplasma in standard layers older than 60 weeks was studied in a French department and the characteristics of infected or free flocks were compared. The genomic profiles of isolates from 36 infected flocks were studied by pulsed-field gel electrophoresis and random amplified polymorphic DNA methods in order to investigate possible routes of transmission. The minimum inhibitory concentrations of antibiotics were determined. Results showed that infection was more frequent in multi-age farms. Egg production and mortality of infected flocks were respectively lower and higher than in non-infected flocks but the differences were not statistically significant. The genomic profiles of isolates were quite homogeneous, a feature which does not facilitate the understanding of routes of transmission. All isolates were susceptible to tetracyclines, macrolides (except erythromycin), spectinomycin and fluoroquinolones.  相似文献   

Application of stable carbon isotope analysis to the detection of testosterone administration to cattle     

Hebestreit M  Flenker U  Buisson C  Andre F  Le Bizec B  Fry H  Lang M  Weigert AP  Heinrich K  Hird S  Schänzer W 《Journal of agricultural and food chemistry》2006,54(8):2850-2858

The use of anabolic substances is prohibited in food-producing animals throughout the European Union. No method is available to reliably detect the misuse of natural hormones in cattle. A method was developed to detect the abuse of testosterone in cattle fattening. Synthesized testosterone is rather depleted in the (13)C/(12)C ratio. Hence, the method is based on gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) analysis of urine. To select testosterone metabolites and endogenous reference compounds (ERC), the concentration of urinary steroids of cattle was investigated. Dehydroepiandrosterone and androst-5ene-3beta,17alpha-diol were chosen as ERCs to show endogenous (13)C/(12)C ratios. Etiocholanolone and 5alpha-androstane-3beta,17alpha-diol were chosen as the most important testosterone metabolites. Other metabolites known from literature like epitestosterone were less promising. In principle, GC/C/IRMS is a nonspecific method because finally carbon dioxide is analyzed. Therefore, a dedicated cleanup procedure for the selected steroids was developed. By means of proposed confidence intervals in the isotopic composition of ERCs and metabolites, the administration of testosterone to cattle could be detected reliably. Differences of up to 11 per thousand on the delta-scale between ERC and testosterone metabolites were found after testosterone administration, whereas endogenous differences did not exceed 2 per thousand.  相似文献