Immune response to Rhodococcus equi ATCC 33701-secreted proteins in mice and identification of immunogenic recombinant proteins by dot-blotting     

Barbey C  Cauchard S  Cauchard J  Laugier C  Hartke A  Petry S 《Research in veterinary science》2012,93(1):172-176

Rhodococcus equi remains a significant pathogen, causing severe pneumonia in foals. The development of vaccines and serologic diagnosis could be greatly facilitated by studying the humoral immune response to this equine pathogen. In this study, a crude extract of R. equi ATCC 33701-secreted proteins combined with the Montanide® ISA70 adjuvant was found to be highly immunogenic in mice with the highest titer of 99,000 on day 42 after the first subcutaneous immunization. This immune response was dependent on the quantity of proteins injected and the presence of adjuvant. By dot-blotting, eight recombinant secreted proteins were identified to react strongly with sera from immunized mice. Of these eight proteins, four were detected as immunogenic only when administered in conjunction with adjuvant. This screening strategy led to the identification of promising new candidates for vaccine development.  相似文献   

Prognostic significance of morphological subtypes in canine malignant lymphomas during chemotherapy     

Ponce F  Magnol JP  Ledieu D  Marchal T  Turinelli V  Chalvet-Monfray K  Fournel-Fleury C 《Veterinary journal (London, England : 1997)》2004,167(2):158-166

The aim of this study was to determine the response of different morphological subtypes of canine lymphoma to a standardized therapeutic protocol. Diagnosis of lymphoma was based on cytohistological analysis and immunophenotyping with antibodies against CD3 and CD79a of an enlarged lymph node or an extranodal mass. Fifty-seven cases were classified according to the updated Kiel classification adapted to the canine species, into 24 B-cell lymphomas (20 centroblastic polymorphic and four Burkitt-type subtypes), and 33 T-cell lymphomas (10 pleomorphic mixed, 10 lymphoblastic, eight unclassifiable high grade plasmacytoid, and five small clear-cell subtypes). All dogs were clinically staged at diagnosis. The protocol used l-asparaginase, vincristine, cyclophosphamide, doxorubicin, and prednisone. First remission duration and overall survival time were evaluated. Although the T-cell phenotype was associated, on the whole, with a poor prognosis, as previously reported in veterinary and human medicine, the study showed significant prognostic differences between the B- and the T-cell subtypes of canine lymphoma and suggests that clinico-morphological characterization of the disease is justified in dogs, as in humans.  相似文献   

Andean Pastoral Women in a Changing World: Opportunities and Challenges     

Corinne Valdivia  Jere L. Gilles  Cecilia Turin 《Herpetological Monographs》2013,35(6):75-81

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Organic and inorganic amendments did not affect microbial community composition in the bulk soil differently but did change the relative abundance of selected taxa     

Corinne Celestina  Peter W. G. Sale  Caixian Tang  Ashley E. Franks 《European Journal of Soil Science》2019,70(4):796-806

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A Necropsy Survey of Neurologic Diseases in 4,319 Horses Examined in Normandy (France) from 1986 to 2006     

Claire Laugier  Jackie TapprestNathalie Foucher  BSc  Corinne Sevin  BSc 《Journal of Equine Veterinary Science》2009

The frequencies of neurologic diseases and various corresponding causes were analyzed in 4,319 Norman horses examined by autopsy between 1986 and 2006. Five hundred forty-three cases of nervous system disorders were detected, or a total prevalence of 12.6%. One hundred eighty-seven of the 543 horses (34.4%) exhibited lesions of a traumatic origin affecting the skull or the vertebral column at different levels. Their frequency was less in foals younger than 1 month of age and higher in horses aged 6 months to 2 years. Eighty-three cases of cervical vertebral compressive myelopathy (CVCM) (15.3%) were identified. Males and geldings were more often affected than females. CVCM was diagnosed mostly in animals aged 6 months to 2 years. Horses affected with lesions in the mid-cervical region (region C3–C5) were significantly younger than those exhibiting more caudal lesions (region C5–C7). Neonatal maladjustment syndrome was diagnosed in 62 foals younger than 3.5 days (4.8% of the causes of death between birth and 1 month) by using clinical or lesional criteria. The prevalence was higher in Thoroughbreds as well as in the miscellaneous breed category and less in French Standardbreds. Bacterial meningoencephalomyelitis and meningitis represented 9.6% of nervous system disorders (52 cases) and most often affected foals aged 1 to 6 months. The main bacteria identified were beta-hemolytic Streptococci , Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, and Actinobacillus sp. The other causes diagnosed were, in decreasing order of frequency: grass sickness or equine dysautonomia (6.3% of neurologic diseases), hepatic encephalopathy syndrome (5.2%), septic vertebral arthritis or osteitis mainly caused by Rhodococcus equi (2.6%), congenital defects of the nervous system (2.4%), equine herpesvirus 1 myeloencephalopathy (2.2%), botulism (2.2%), malformations of the vertebral column other than CVCM (2%), and neighboring polyneuritis associated with guttural pouch mycosis (1.5%).  相似文献   

Recreational Multifunctionality and its implications for agroforestry diffusion   总被引：1，自引：1，他引：0  

Carla Barbieri  Corinne Valdivia 《Agroforestry Systems》2010,79(1):5-18

Recreational Multifunctionality refers to those farms providing at least one recreational service to members of the farm household or the public. Based on the types of recreational services offered, two types of landowners have been identified: Productivists and Ruralists. They differ on their extent of Recreational Multifunctionality, farm household attributes and farm representation. Both types of landowners also have different behavior and understanding of agroforestry. This study builds on the Recreational Multifunctionality construct to identify message content, channels and agencies that can better facilitate the diffusion of agroforestry. In 2006, 353 randomly selected landowners from Missouri were interviewed and clustered in two groups: Productivists (38.0%) and Ruralists (62.0%). These groups were examined to identify differences regarding their perceptions of being a good farmer (i.e., message content); their preferred source of information (i.e., communication channel); and their preferred agencies for learning purposes (i.e., communication agencies). Study results show differences between Productivists and Ruralists in all three aspects of diffusion examined (i.e., message content, channels and agencies), suggesting that all landowners should not be approached in the same way when promoting agroforestry. Messages conveyed to Productivists should emphasize the economic benefits of agroforestry, while messages to Ruralists should emphasize the conservation benefits of agroforestry. Touring farms with active tree management is a good way to disseminate agroforestry practices among Productivists while state and federal extension agents appear to be more suitable to approach Ruralists. Both groups can effectively be reached using printed materials, especially through official conservation magazines.  相似文献   

Waldenström's macroglobulinemia in a dog with a bleeding diathesis     

Jaillardon L  Fournel-Fleury C 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2011,40(3):351-355

Abstract: An 11‐year‐old female spayed Whippet dog was referred to the Veterinary Hospital of the National Veterinary School of Lyon with a 3‐month history of chronic bleeding, cutaneous masses suggestive of hematomas, and hemorrhagic diarrhea. Laboratory abnormalities included leukopenia with neutropenia and lymphopenia, rouleaux formation, marked hyperproteinemia with hyperglobulinemia and normoalbuminemia, hypercalcemia, markedly increased serum urea concentration, mildly increased creatinine concentration, and proteinuria. Hemostatic screening tests showed marked prolongation of thrombin time, increased concentrations of fibrin(ogen) degradation products and D‐dimers, and slightly prolonged activated partial thromboplastin time. Serum and urine protein electrophoresis revealed a monoclonal peak identified as IgM by immunoelectrophoresis and an M‐spike, respectively. Cytologic evaluation of bone marrow revealed a hypocellular sample with lymphoplasmacytic cells comprising approximately 50% of all nucleated cells. These findings were consistent with a diagnosis of Waldenström's macroglobulinemia with secondary bleeding diathesis. Treatment with prednisone and chlorambucil was initiated, and the dog had a good response.  相似文献   

User-friendly extraction and multistage tandem mass spectrometry based analysis of lipid-linked oligosaccharides in microalgae     

Pierre-Louis Lucas  Rodolphe Dumontier  Corinne Loutelier-Bourhis  Alain Mareck  Carlos Afonso  Patrice Lerouge  Narimane Mati-Baouche  Muriel Bardor 《Plant methods》2018,14(1):107

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Six chemicals with animal repellent or insecticide properties are screened for phytotoxic effects on the germination and viability of ash, birch, Corsican pine and sycamore seeds   总被引：1，自引：0，他引：1  

Gosling  Peter G.; Baker  Corinne 《Forestry》2004,77(5):397-403

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Etude experimentale du transport de Pinoculum de Ceratocystis fimbriata f. platani par ľeau ?une riviere     

C. Grosclaude  R. Olivier  J.-C. Pizzuto  Corinne Romiti 《Forest Pathology》1991,21(3):168-171

Dissemination of Ceratocystis fimbriata f. platani inoculum by river water . C. fimbriata was detected up to 108 m downstream from pieces of diseased plane tree placed in a fixed site in a river in Southern France. Assessment of C. fimbriata was realized by a trapping method with healthy wood.  相似文献