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51.
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Neonatal haemolytic disease was diagnosed in a Bos indicus cross bred beef herd in south-east Queensland. The evidence suggested that this was due to B. argentina vaccination for babesiosis while non-pregnant or less than one month pregnant. The cows received 5 or 6 vaccinations during their breeding life, which indicated that antibody levels can persist for long periods. The autopsy, histopathological and haematological findings indicated that the mechanism was disseminated intra-vascular coagulation which occurred in calves dying within 24 hours of birth. This resulted in fibrin deposition in pulmonary capillaries and severe pulmonary oedema. 相似文献
53.
Molecular coupling of Xist regulation and pluripotency 总被引:2,自引:0,他引:2
Navarro P Chambers I Karwacki-Neisius V Chureau C Morey C Rougeulle C Avner P 《Science (New York, N.Y.)》2008,321(5896):1693-1695
During mouse embryogenesis, reversion of imprinted X chromosome inactivation in the pluripotent inner cell mass of the female blastocyst is initiated by the repression of Xist from the paternal X chromosome. Here we report that key factors supporting pluripotency-Nanog, Oct3/4, and Sox2-bind within Xist intron 1 in undifferentiated embryonic stem (ES) cells. Whereas Nanog null ES cells display a reversible and moderate up-regulation of Xist in the absence of any apparent modification of Oct3/4 and Sox2 binding, the drastic release of all three factors from Xist intron 1 triggers rapid ectopic accumulation of Xist RNA. We conclude that the three main genetic factors underlying pluripotency cooperate to repress Xist and thus couple X inactivation reprogramming to the control of pluripotency during embryogenesis. 相似文献
54.
Rederstorff E Fatimi A Sinquin C Ratiskol J Merceron C Vinatier C Weiss P Colliec-Jouault S 《Marine drugs》2011,9(2):224-241
Polysaccharides are highly heat-sensitive macromolecules, so high temperature treatments are greatly destructive and cause considerable damage, such as a great decrease in both viscosity and molecular weight of the polymer. The technical feasibility of the production of exopolysaccharides by deep-sea bacteria Vibrio diabolicus and Alteromonas infernus was previously demonstrated using a bioproduct manufacturing process. The objective of this study was to determine which sterilization method, other than heat sterilization, was the most appropriate for these marine exopolysaccharides and was in accordance with bioprocess engineering requirements. Chemical sterilization using low-temperature ethylene oxide and a mixture of ionized gases (plasmas) was compared to the sterilization methods using gamma and beta radiations. The changes to both the physical and chemical properties of the sterilized exopolysaccharides were analyzed. The use of ethylene oxide can be recommended for the sterilization of polysaccharides as a weak effect on both rheological and structural properties was observed. This low-temperature gas sterilizing process is very efficient, giving a good Sterility Assurance Level (SAL), and is also well suited to large-scale compound manufacturing in the pharmaceutical industry. 相似文献
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Karim Senni Farida Gueniche Sylvie Changotade Dominique Septier Corinne Sinquin Jacqueline Ratiskol Didier Lutomski Gaston Godeau Jean Guezennec Sylvia Colliec-Jouault 《Marine drugs》2013,11(4):1351-1369
Biopolymers produced by marine organisms can offer useful tools for regenerative medicine. Particularly, HE800 exopolysaccharide (HE800 EPS) secreted by a deep-sea hydrothermal bacterium displays an interesting glycosaminoglycan-like feature resembling hyaluronan. Previous studies demonstrated its effectiveness to enhance in vivo bone regeneration and to support osteoblastic cell metabolism in culture. Thus, in order to assess the usefulness of this high-molecular weight polymer in tissue engineering and tissue repair, in vitro reconstructed connective tissues containing HE800 EPS were performed. We showed that this polysaccharide promotes both collagen structuring and extracellular matrix settle by dermal fibroblasts. Furthermore, from the native HE800 EPS, a low-molecular weight sulfated derivative (HE800 DROS) displaying chemical analogy with heparan-sulfate, was designed. Thus, it was demonstrated that HE800 DROS mimics some properties of heparan-sulfate, such as promotion of fibroblast proliferation and inhibition of matrix metalloproteinase (MMP) secretion. Therefore, we suggest that the HE800EPS family can be considered as an innovative biotechnological source of glycosaminoglycan-like compounds useful to design biomaterials and drugs for tissue engineering and repair. 相似文献
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Raphaëlle Mouttet Abraham Escobar‐Gutiérrez Magali Esquibet Laurent Gentzbittel Didier Mugniéry Philippe Reignault Corinne Sarniguet Philippe Castagnone‐Sereno 《Pest management science》2014,70(7):1017-1022
In Europe, the stem and bulb nematode Ditylenchus dipsaci has been listed as a quarantine pest by EPPO: without any control, it may cause complete failure of alfalfa crops. Movement of nematodes associated with seeds is considered to be the highest‐risk pathway for the spread of this pest. Since the 2010 official withdrawal of methyl bromide in Europe, and in the absence of any alternative chemical, fumigation of contaminated seed batches is no longer possible, which makes the production of nematode‐free alfalfa seeds difficult to achieve and leads to unmarketable seed batches. Thermotherapy is being considered as a realistic alternative strategy, but its efficiency still remains to be validated. The combination of the currently available methods (i.e. use of resistant cultivars, seed production according to a certification scheme, mechanical sieving, seed batch inspection) could significantly reduce the likelihood of seed contamination. However, it does not guarantee a total eradication of the nematode. Although it is already widely distributed all over Europe, reclassification of D. dipsaci as a regulated non‐quarantine pest to reduce the possibility of further introductions and the rate of spread of this pest appears to be a risky strategy because of the lack of up‐to‐date documented data to evaluate damage thresholds and determine acceptable tolerance levels. [[ArtCopyrightmsg]] 相似文献
59.
Background
The repeated weekly subculture of plant cell suspension is labour intensive and increases the risk of variation from parental cells lines. Most of the procedures to preserve cultures are based on controlled freezing/thawing and storage in liquid nitrogen. However, cells viability after unfreezing is uncertain. The long-term storage and regeneration of plant cell cultures remains a priority. 相似文献60.
Jason Holderieath Corinne Valdivia Larry Godsey Carla Barbieri 《Agroforestry Systems》2012,86(3):345-353
Global carbon trading may present a unique opportunity to change the rural landscape by allowing landowners to make an environmental impact with financial incentives. By enticing point source polluters to trade to an optimal level of pollution by offsetting their emissions with compatible carbon reduction projects, markets are able to facilitate a cleaner environment. Agroforestry provides a set of practices that can sequester carbon with managed tree and crop plantings. However, the initial lack of financial resources has been an obstacle to its adoption in the United States. This paper explores the potential for carbon offset trading to provide an added incentive to adopt agroforestry practices. Chicago Climate Exchange carbon sequestration projects are used as a baseline assessment, and the requirements that Missouri landowners would need to do in terms of contracting and ownership are identified. Data from landowners in central Missouri and the Ozarks (353 individuals) are used to determine characteristics of potentially interested landowners in agroforestry. A model to evaluate agroforestry profitability scenarios is used to compare the added carbon credit trading revenue to traditional alternate uses for the property. The findings from this analysis indicate that in the current context carbon trading does not provide an added incentive value for Missouri landowners to adopt either silvopasture or alley cropping practices because of the low magnitude of annual return. 相似文献