Alpha‐Linolenic Acid Supplementation in Tris Extender Can Improve Frozen–Thawed Bull Semen Quality          下载免费PDF全文

A Kaka  H Wahid  Y Rosnina  N Yimer  AM Khumran  AA Behan  M Ebrahimi 《Reproduction in domestic animals》2015,50(1):29-33

The study was conducted to evaluate the effects of α ‐linolenic acid (ALA) on frozen–thawed quality and fatty acid composition of bull sperm. For that, twenty‐four ejaculates obtained from three bulls were diluted in a Tris extender containing 0 (control), 3, 5, 10 and 15 ng/ml of ALA. Extended semen was incubated at 37°C for 15 min, to allow absorption of ALA by sperm cell membrane. The sample was chilled for 2 h, packed into 0.25‐ml straws and frozen in liquid nitrogen for 24 h. Subsequently, straws were thawed and evaluated for total sperm motility (computer‐assisted semen analysis), membrane functional integrity (hypo‐osmotic swelling test), viability (eosin‐nigrosin), fatty acid composition (gas chromatography) and lipid peroxidation (thiobarbituric acid‐reactive substances (TBARS)). A higher (p < 0.05) percentage of total sperm motility was observed in ALA groups 5 ng/ml (47.74 ± 07) and 10 ng/ml (44.90 ± 0.7) in comparison with control (34.53 ± 3.0), 3 ng/ml (34.40 ± 2.6) and 15 ng/ml (34.60 ± 2.9). Still, the 5 ng/ml ALA group presented a higher (p < 0.05) percentage of viable sperms (74.13 ± 0.8) and sperms with intact membrane (74.46 ± 09) than all other experimental groups. ALA concentration and lipid peroxidation in post‐thawed sperm was higher in all treated groups when compared to the control group. As such, the addition of 5 ng/ml of ALA to Tris extender improved quality of frozen–thawed bull spermatozoa.  相似文献   

Early Cleavage of Handmade Cloned Buffalo (Bubalus bubalis) Embryos is an Indicator of Their Developmental Competence and Quality          下载免费PDF全文

S Kaith  M Saini  AK Raja  AA Sahare  B Jyotsana  P Madheshiya  P Palta  MS Chauhan  RS Manik  SK Singla 《Reproduction in domestic animals》2015,50(2):214-220

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Integrating local ecological knowledge,citizen science and long‐term historical data for endangered species conservation: Additional records of angel sharks (Chondrichthyes: Squatinidae) in the Mediterranean Sea     

Ioannis Giovos  Vasilis‐Orestis Stoilas  Sara AA Al‐Mabruk  Nikolaos Doumpas  Philippos Marakis  Mary Maximiadi  Dimitrios Moutopoulos  Periklis Kleitou  Ioannis Keramidas  Francesco Tiralongo  Alessandro de Maddalena 《水产资源保护:海洋与淡水生态系统》2019,29(6):881-890

Pathological changes in the mammary gland and biochemical changes in milk of the goat following oral dosing with leaf of the Avocado (Persea americana)     

AL CRAIGMILL  AA SEAWRIGHT  T. MATTILA †  AJ FROST 《Australian veterinary journal》1989,66(7):206-211

Two varieties of avocado leaves (Persea americana var Guatemalan and var Mexican) were administered to lactating goats. The Mexican variety was without effect. The Guatemalan variety in doses exceeding 20 g fresh leaf per kg bodyweight, produced damage to the mammary gland with decreased milk production. The lesions were characterised by oedema and reddening, with clots in the large ducts. Microscopically, there was widespread degeneration and necrosis of the secretory epithelium, the necrotic cells sloughing into the lumen. There was no significant cellular inflammatory response. Concentrations of antitrypsin in the milk, indicating changes in vascular permeability, increased rapidly 15 h after a single high dose, coinciding with palpable oedema. Concentrations of NAGase, indicating cell damage, increased after 24 h. Goats given multiple doses followed a similar pattern but the initial response was delayed. The toxic principle, and its mode of action in selectively damaging mammary secretory cells, remains to be determined.  相似文献   

Superior check ligament desmotomy for treatment of superficial digital flexor tendonitis in Thoroughbred and Standardbred horses     

IC FULTON  AA MacLEAN†  JL O'RIELLY†  S. CHURCH† 《Australian veterinary journal》1994,71(8):233-235

Summary Bilateral superior check ligament desmotomy was performed on 31 Thoroughbred and 17 Standardbred horses as the sole method of treatment for superficial digital flexor tendonitis. Horses resumed racing between 6 and 19 months after surgery. Horses that were able to compete in 5 or more races without injury to the tendon again were considered to have had a successful return to racing. Twenty-five Thoroughbreds were suitable for later study and 13 of these (52%) raced on 5 or more occasions. Fifteen Standardbreds were suitable for later study and 10 of these (66%) raced on 5 or more occasions. Within the racing industry it is generally thought that about 20 to 30% of horses with superficial digital flexor tendonitis can return to racing after a prolonged rest. The results of this study suggest that bilateral superior check ligament desmotomy may improve the prognosis for a horse returning to racing after injury to the superficial digital flexor tendon.  相似文献   

Relationship between Daily Milk Yield and Persistency of Hungarian Holstein Friesian through Selection Index and Sub-indices     

AA Amin  S Töth  T Gere 《Reproduction in domestic animals》1997,32(4):207-212

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Phenylbutazone in racing Greyhounds: plasma and urinary residues 24 and 48 hours after a single intravenous administration     

PC MILLS  JC NG†  KV SKELTON†  AA SEAWRIGHT  DE AUER† 《Australian veterinary journal》1995,72(8):304-308

SUMMARY The concentrations of phenylbutazone (PBZ), oxyphenbutazone (OPBZ) and gammahydroxyphenylbutazone (OHPBZ) in plasma and urine from 50 Greyhounds 24 and 48 h after the intravenous administration of a single dose of PBZ (30 mg/kg) were measured. The 24 h plasma concentrations of OPBZ and OHPBZ, the 48 h plasma concentration of OHPBZ and the 24 h urinary concentration of PBZ were normally distributed, while log transformations were required before the 24 h plasma concentration of PBZ and the 24 and 48 h urinary concentrations of OPBZ and OHPBZ became normally distributed. The 95%, 99%, 99.9% and 99.99% upper predicted confidence intervals for both 24 h and 48 h plasma and urinary concentrations demonstrated wide potential variation in the concentration of the analytes should PBZ be administered to Greyhounds. The 24 h plasma and urinary concentrations of PBZ were weakly correlated, but no similar relationship existed for OPBZ or OHPBZ. The urinary concentrations of each analyte were not affected by the trainer or sex of the Greyhound or the urinary pH. We conclude that it would be impossible to predict the timing of the PBZ administration or the plasma concentration of PBZ from the measurement of the concentration of PBZ in a single sample of urine.  相似文献   

Carbon structures with three-dimensional periodicity at optical wavelengths   总被引：1，自引：0，他引：1  

AA Zakhidov  RH Baughman  Z Iqbal  C Cui  I Khayrullin  SO Dantas  J Marti  VG Ralchenko 《Science (New York, N.Y.)》1998,282(5390):897-901

Porous carbons that are three-dimensionally periodic on the scale of optical wavelengths were made by a synthesis route resembling the geological formation of natural opal. Porous silica opal crystals were sintered to form an intersphere interface through which the silica was removed after infiltration with carbon or a carbon precursor. The resulting porous carbons had different structures depending on synthesis conditions. Both diamond and glassy carbon inverse opals resulted from volume filling. Graphite inverse opals, comprising 40-angstrom-thick layers of graphite sheets tiled on spherical surfaces, were produced by surface templating. The carbon inverse opals provide examples of both dielectric and metallic optical photonic crystals. They strongly diffract light and may provide a route toward photonic band-gap materials.  相似文献   

Animal Welfare: Focusing on the Future,OIE Scientific and Technical Review Volume 33(1)     

AA Cullum BSc.  BVM&S  MACVSc 《New Zealand veterinary journal》2013,61(2)

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Treatment of anovulatory anoestrous postpartum dairy cows with a gonadotropin-releasing hormone (GnRH), prostaglandin F2𝛂, GnRH regimen or with progesterone and oestradiol benzoate     

S McDougall  AA Cullum  FM Anniss  FM Rhodes 《New Zealand veterinary journal》2013,61(5):168-172

AIM: To compare 2 treatments for anovulatory anoestrus (AA) in postpartum dairy cows. The treatments were combinations of gonadotropin-releasing hormone (GnRH) and prostaglandin F_2𝛂 (PG) or progesterone (P4) and oestradiol benzoate (ODB).

METHODS: Forty AA cows from each of 5 herds were blocked by age (2 or >2 years old) and randomly assigned to 1 of 2 treatments. The first group (GPG) were treated with 250 𝛍g of a GnRH analogue, gonadorelin, followed 7 days later by 15 mg of the PG analogue, luprostiol. Two days later the cows were injected with 250 𝛍g of gonadorelin. Cows were artificially inseminated 16–24 h after the second GnRH injection. The second group (P4+ODB) were treated with an intravaginal P4 releasing device for 6 days, followed 24 h after device removal by injection of 1 mg of ODB. Cows were pregnancy tested 35–40 days after the initial insemination and twice again at 6–8 week intervals thereafter.

RESULTS: There was no significant difference between P4+ODB and GPG groups in the percentage of cows submitted for insemination in the first 7 days (94.0% vs 100% for P4+ODB vs GPG, respectively; p>0.3), in conception rate to first insemination within the first 7 days (43.6% vs 35.0% for P4+ODB vs GPG, respectively; p>0.2), in the percentage of cows conceiving in the first 28 days of the breeding period (68.0% vs 58.3%, P4+ODB vs GPG, respectively; p>0.1), or in median interval from the end of treatment to conception (20 vs 21days;p>0.1).

CONCLUSIONS: No differences in the reproductive performance of AA cows treated with either P4+ODB or GPG were detected. However, given the small number of animals enrolled, further data are required before the GPG protocol can be recommended for treatment of AA cows.  相似文献