G02Identification of functional TRPV1 vanilloid receptor in MCF-7 cells     

R. BARBERO  P. BADINO  B. CUNIBERTI  A. MIOLO  R. ODORE  V. DI MARZO  & G. RE 《Journal of veterinary pharmacology and therapeutics》2006,29(S1):203-204

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G03Aliamides modulate skin mast cell degranulation in dogs and cats     

A. MIOLO  G. RE  R. BARBERO  M. GIORGI  R. LEOTTA  S. AUXILIA  C. NOLI  & F. ABRAMO 《Journal of veterinary pharmacology and therapeutics》2006,29(S1):204-205

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Progesterone and Caspase-3 Activation in Equine Cyclic Corpora Lutea     

G Ferreira-Dias  L Mateus  AS Costa  S Solá  RM Ramalho  RE Castro  CMP Rodrigues 《Reproduction in domestic animals》2007,42(4):380-386

Soon after ovulation, the newly formed corpus luteum (CL) starts secreting progesterone (P(4)), necessary for implantation. The CL, an ovarian transient endocrine organ, undergoes growth and regression throughout its life span. The objective of this study was to evaluate if caspase-3 mediates cell death in the equine cyclic luteal structures and relate it to luteal endocrine function. Blood and luteal tissue were collected during the breeding season after slaughter from 38 randomly assigned cycling mares. Luteal tissues were classified as corpora haemorrhagica (CH; n = 7); mid luteal phase corpora lutea (Mid-CL; n = 17); late or regressing corpora lutea (Late-CL; n = 9) and corpora albicans (CA; n = 5). Plasma P(4) concentration, determined by radioimmunoassay, showed a significant increase from CH to Mid-CL (p < 0.001), followed by a decrease to Late-CL (p < 0.001) and CA (p < 0.001). Caspase-3 processing and poly (ADP) ribose polymerase (PARP) degradation were assessed by western blotting. Active caspase-3 was twofold increased in Mid-CL, Late-CL and CA as compared with CH (p < 0.05). Immunocytochemistry also showed a significant increase in caspase-3 expression in large luteal cells in all structures when compared with CH (p < 0.05). Consistently, the endogenous caspase-3 substrate, PARP, was markedly degraded from CH to CA (p < 0.05). In fact, the ratio of full-length to degraded PARP showed a significant decrease from CH to Mid-CL, Late-CL and CA (p < 0.05). Finally, the decrease in P(4) from Mid- to Late-CL coincided with no further increases in apoptosis. In conclusion, these results suggest that the effector caspase-3 of apoptosis, might play an important role during luteal tissue involution in the mare, even though its relationship with P(4) remains to be elucidated.  相似文献   

抓低改促高产求发展——双峰乡实施毛竹丰产经营的调查     

宋军华  钟日生 《江西林业科技》2001,(Z1)

双峰乡是中国“毛竹之乡”——宜丰县的毛竹重点产区.在对毛竹林的经营中,双峰乡通过全面规划、分类改造,改革机制、多种管理方式经营,建设样板、典型带动推广,以及基地建设与竹材深加工开发相结合等一系列措施,使这个“双料”的毛竹之乡真正发挥了资源优势,实现了毛竹丰产高效,促进了地方经济发展.调查总结了其成功的竹林经营管理经验,还介绍了向全县推广的措施方法.  相似文献   

A comparative study of single and multiple respiratory infections in the chicken: multiple infections (with Mycoplasma gallisepticum, Newcastle disease virus, and infectious bronchitis virus)     

R E Corstvet  W W Sadler 《American journal of veterinary research》1966,27(121):1703-1720

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Effect of breed of cattle on transmission rate and innate resistance to infection with Babesia bovis and B bigemina transmitted by Boophilus microplus     

RE BOCK  TG KINGSTON  AJ de  VOS 《Australian veterinary journal》1999,77(7):461-464

OBJECTIVE: To assess the effect of breed of cattle on the transmission rates of and innate resistance to Babesia bovis and B bigemina parasites transmitted by Boophilus microplus ticks. DESIGN: Groups of 56 purebred B indicus and 52 B indicus cross B taurus (50%, F1 generation) steers were placed in a paddock seeded with and also naturally infested with B microplus which were the progeny of females ticks fed on B taurus cattle specifically infected with a virulent isolate of B bovis. The cattle were placed in the infested paddock 50 days after seeding had started. PROCEDURE: Cattle were inspected from horseback daily for 50 days. Clinically ill cattle were brought to yards and assessed by monitoring fever, depression of packed-cell volume, parasitaemia and severity of clinical signs. Any animals that met preset criteria were treated for babesiosis. Blood samples were collected from all cattle on day 28, 35 and 42 after exposure and antibodies to Babesia spp and packed cell volume measured. RESULTS: All steers, except for one crossbred, seroconverted to B bovis and B bigemina by day 35 and 75% of the crossbred steers showed a maximum depression in packed cell volume of more than 15% due to infection with Babesia spp compared with only 36% of the B indicus group. Ten of the 52 crossbreds and 1 of the 56 B indicus steers showed severe clinical signs. Two of the crossbreds required treatment of which one died 2 weeks after initial treatment. CONCLUSIONS: Pure-bred B indicus cattle have a high degree of resistance to babesiosis, but crossbred cattle are sufficiently susceptible to warrant the use of preventive measures such as vaccination. Transmission rates of B bovis and B bigemina to B indicus and crossbred cattle previously unexposed to B microplus were the same.  相似文献   

Magnetic microstructure of magnetotactic bacteria by electron holography     

RE Dunin-Borkowski  MR McCartney  RB Frankel  DA Bazylinski  M Posfai  PR Buseck 《Science (New York, N.Y.)》1998,282(5395):1868-1870

Off-axis electron holography in the transmission electron microscope was used to correlate the physical and magnetic microstructure of magnetite nanocrystals in magnetotactic bacteria. The magnetite crystals were all single magnetic domains, and the magnetization directions of small superparamagnetic crystals were constrained by magnetic interactions with larger crystals in the chains. Shape anisotropy was found to dominate magnetocrystalline anisotropy in elongated crystals. A coercive field between 300 and 450 oersted was determined for one chain.  相似文献   

设施老汉瓜采后干制技术研究     

杜娟  热比古丽·哈力克  沙吾提·阿布拉江  廖新福 《农产品加工．学刊》2020,(4):30-32

以设施老汉瓜为试材,采用不同成熟度、前处理、预处理温度后进行干制,测定含水率、失重率、色差等指标变化。相同的干燥温度、时间条件下,6个成熟度、2种不同的前处理、5种预处理温度的设施老汉瓜的失重率、最终含水率、总色差值在干制期内变化不同。结果表明,不同成熟度的设施老汉瓜,干制期的预处理温度、前处理和烘制温度差距,影响老汉瓜在干制过程中水分的散失。采用不同处理方式至完全干燥,可溶性固形物含量11%的设施老汉瓜,瓜干失重率、含水率、瓜干总色差值ΔE变化略慢,较好地保持瓜干的品质,适宜于老汉瓜的加工。  相似文献   

Failure of imidocarb dipropionate to clear experimentally induced Ehrlichia canis infection in dogs     

Eddlestone SM  Neer TM  Gaunt SD  Corstvet R  Gill A  Hosgood G  Hegarty B  Breitschwerdt EB 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2006,20(4):840-844

The recommended treatment for canine ehrlichiosis is tetracycline or its analog doxycycline, although recent reports have documented ineffective clearing of Erchlichia canis after doxycycline administration. Imidocarb dipropionate is used as an alternative treatment to tetracycline or is used in conjunction with doxycycline. The effectiveness of imidocarb dipropionate in clearing Ehrlichia species from the blood and tissues of dogs with E. canis infection has not been thoroughly evaluated. Fifteen dogs were experimentally infected with E. canis. Ten dogs were treated with imidocarb dipropionate (6.6 mg/kg, IM, 2 injections given 2 weeks apart). Five infected control dogs were not treated. Blood samples from all 15 dogs were E. canis DNA positive by PCR assay by 3 weeks after inoculation (PI), and E. canis antibodies were detected by IFA assay by 1 week PI. Blood platelet counts in all dogs were below the reference interval by 4 weeks PI. E. canis DNA was detected in bone marrow and splenic aspirates by PCR assay 4 weeks PI but not before infection. Bone marrow aspirates were E. canis DNA positive by PCR assay in 14/15 dogs, and splenic aspirates were E. canis DNA positive by PCR assay in 13/15 dogs. Blood samples from all treated and control dogs remained positive for E. canis DNA by PCR assay, and platelet counts remained below preinoculation values 13 weeks PI (6 weeks after 2nd treatment). As administered in this study, imidocarb dipropionate did not clear experimental E. canis infection in dogs.  相似文献   

水分胁迫下4种冷季型草坪草抗旱性比较研究   总被引：1，自引：0，他引：1  

初晓辉  岳信龙  任健  陈功  单贵莲 《草原与草坪》2012,32(4):15-19

采用盆栽育苗法,研究了4种冷季型草坪草在不同水分胁迫下的抗旱性,结果表明:1)随着水分胁迫程度的加深,4种冷季型草坪草的外观质量和叶片相对含水量显著下降,细胞质膜透性、MDA含量和Pro含量上升。2)模糊数学综合评价结果表明,4种冷季型草坪草种抗旱性能由强到弱的顺序为:高羊茅>多年生黑麦草>草地早熟禾>匍匐翦股颖。  相似文献