Canine leishmaniasis chemotherapy: dog's clinical condition and risk of Leishmania transmission     

João A  Pereira MA  Cortes S  Santos-Gomes GM 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2006,53(10):540-545

The aim of this study was to investigate whether treatment against canine leishmaniasis reduced the presence of Leishmania in the healthy skin of dogs, affecting the capacity of parasite transmission. A total of 37 dogs from an endemic region of leishmaniasis were studied. Thirteen symptomatic animals revealed parasites in the bone marrow and eight had also in the skin. Five of the 22 dogs that had been treated with meglumine antimoniate alone, meglumine antimoniate or trifluralin followed by allopurinol or just with allopurinol had the parasite in bone marrow but none showed Leishmania in the skin. One dog that was treated only with aminosidine was polisymptomatic and had parasites in bone marrow and skin. The different treatments used in this study did not completely eliminate the parasite allowing relapses to occur when the treatment is discontinued, but the use of meglumine antimoniate or allopurinol, alone or combined may improve dogs clinical condition and reduce or eliminate the parasite from the skin decreasing the probability of Leishmania transmission.  相似文献   

Application of multielement stable isotope ratio analysis to the characterization of French, italian, and spanish cheeses   总被引：6，自引：0，他引：6  

Camin F  Wietzerbin K  Cortes AB  Haberhauer G  Lees M  Versini G 《Journal of agricultural and food chemistry》2004,52(21):6592-6601

The stable isotope ratios (delta13C, delta15N, and delta34S of casein and delta13C and delta18O of glycerol) measured by IRMS of French, Italian, and Spanish cheeses are presented and discussed. Variability factors such as animal-feeding regimen, geographical origin, and climatic and seasonal conditions were studied to check the possibilities of cheese characterization offered by each isotopic parameter. Delta13C values of both casein and glycerol appeared to be strongly correlated to the amount of maize in the animal diet. Delta15N and delta34S of casein proved to be mostly influenced by the geoclimatic conditions of the area (aridity, closeness to the sea, altitude). Delta18O of glycerol was more dependent on the geographical origin of the cheeses and on climatic/seasonal parameters. By applying a multivariate stepwise canonical discriminant analysis, good discrimination possibilities for the different European cheeses were obtained, confirmed by the classification analysis, when >90% of the samples were correctly reclassified.  相似文献   

Dynamics of Moraxella bovis infection and humoral immune response to bovine herpes virus type 1 during a natural outbreak of infectious bovine keratoconjunctivitis in beef calves     

MV Zbrun  GC Zielinski  HC Piscitelli  C Descarga  LA Urbani 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(4):347-352

Infectious bovine keratoconjunctivitis (IBK) is an acute disease caused by Moraxella bovis (Mb). Several factors may predispose animals to an IBK outbreak; one commonly observed is infection with bovine herpes virus type 1 (BHV-1). The aim of this study was to investigate the dynamics of BHV-1 virus infection and its relation with clinical cases of IBK in weaned calves from a beef herd with a high prevalence of lesions caused by Mb. Sampling was carried out in six stages and included conjunctival swabs for isolating Mb as well as blood samples for identifying antibodies specific for BHV-1. A score for IBK lesions after observing each eye was determined. The findings of this study showed a high prevalence of BHV-1 virus infection (100% of animals were infected at the end of the trial); 67% of animals were culture-positive for Mb, but low rates of clinical IBK (19% of calves affected) were detected at the end of the trial. These results suggest that infection with BHV-1 did not predispose these animals to IBK, and that Mb infection produced clinical and subclinical disease in the absence of BHV-1 co-infection.  相似文献   

Resolution of spontaneous hemoabdomen secondary to peliosis hepatis following surgery and azithromycin treatment in a Bartonella species infected dog          下载免费PDF全文

Steven T. Berkowitz DVM  Kristi M. Gannon DVM  DACVECC  Carol A. Carberry DVM  DACVS  Yonaira Cortes DVM  DACVECC 《Journal of Veterinary Emergency and Critical Care》2016,26(6):851-857

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Does serum anti‐Müllerian hormone levels always discriminate presence of the ovaries in adult bitches? Comparison of two ELISA kits          下载免费PDF全文

I Pir Yagci  M Pekcan  IM Polat  H Kalender  HC Macun 《Reproduction in domestic animals》2016,51(6):910-915

Anti‐Müllerian hormone (AMH) is produced in the ovary, and thus, it is an excellent marker of follicle pool in females. Current interest is the clinical use of this parameter as a biomarker to assess presence or absence of an intact ovary and to diagnose ovarian remnant syndrome (ORS) following incomplete ovariohysterectomy (OHE) in bitches. The aim of this study was to evaluate serum AMH concentrations in bitches (n = 34) before and after OHE using two different commercial ELISA kits, one of which is based on detecting human AMH and the other is based on detecting human AMH and the other specified for canine AMH. Furthermore, serum AMH levels were also measured in six ORS cases to compare the diagnostic utility of the two different ELISA kits. Serum AMH concentrations measured using the human and canine kit prior to and after OHE were 0.32 ± 0.24, 0.006 ± 0.22 ng/ml (p < .001) and 12.08 ± 22.81, 9.55 ± 15.42 ng/ml (p = .868), respectively. Thus, the canine‐based kit was not able to reveal the significant drop in serum AMH levels. In conclusion, the human‐based ELISA kits successfully detected the drop in serum AMH concentrations. Reliable results can only be achieved from well‐designed ELISA kits, and AMH levels might be a useful diagnostic tool for the evaluation of presence or absence of ovaries as well as for the detection of ORS cases in bitches.  相似文献   

Ecology of southern European pearl mussels (Margaritifera margaritifera): first record of two new populations on the rivers Terva and Beça (Portugal)     

Simone Varandas  Manuel Lopes‐Lima  Amílcar Teixeira  Mariana Hinzmann  Joaquim Reis  Rui Cortes  Jorge Machado  Ronaldo Sousa 《水产资源保护:海洋与淡水生态系统》2013,23(3):374-389

Effects of She Xiang Bao Xin Wan (SXBXW) on the proliferation of primary cultured human umbilical artery vascular smooth muscle cells induced by endothelin-1     

CHE Xian-da  ZHANG Qing-gang  QIAN Lin-yan  ZHANG Xiao-hui  GAO Rui-lan  Beng HC  Simon XL  Xinmai J 《园艺学报》2010,26(2):222-226

AIM: To observe the effects of She Xiang Bao Xin Wan (SXBXW) of Chinese patent medicine on the proliferation of primary cultured vascular smooth muscle cells (VSMCs) from human umbilical artery and stimulated by endothelin-1 (ET-1) in vitro. METHODS: The proliferation cell models of primary cultured VSMCs were established by ET-1 stimulation. Six groups in the experiment were divided into control group; ET-1 group; ET-1+SXBXW 0.25 g/L; ET-1+SXBXW 0.5 g/L; ET-1+SXBXW 1.0 g/L and ET-1+SXBXW 2.0 g/L groups, respectively. The proliferation induced by ET-1 and the suppression mediated by SXBXW on VSMCs were measured by MTT method. The inhibitory rate and the cytotoxicity of SXBXW were detected by lactate dehydrogenase colorimetry and trypan blue exclusion tests. The effect of ET-1 and SXBXW on the cell proliferation cycle was analyzed by flow cytometry. RESULTS: Compared to control group, ET-1 significantly enhanced the proliferation of VSMCs (P<0.01). However, a certain dose of SXBXW inhibited effectively the proliferation of VSMCs induced by ET-1 in a dose-dependent manner (P<0.01). Meanwhile, SXBXW showed no influenced on both the number of living cells and the release of lactate dehydrogenase, although it inhibited the proliferation of VSMCs, indicating that SXBXW was no cytotoxicitic effect on VSMCs. ET-1 enhanced the proliferation of VSMCs by means of promoting the transition of the cell cycle from G1 phase to S phase. However SXBXW significantly inhibited the proliferation mediated by ET-1. CONCLUSION: SXBXW plays the role in suppressing VSMCs proliferation induced by ET-1. The mechanism may be involved in blocking the cell cycle from G1 phase into S phase.  相似文献   

Cytokine expression during the outcome of canine experimental infection by Leishmania infantum   总被引：2，自引：0，他引：2  

Santos-Gomes GM  Rosa R  Leandro C  Cortes S  Romão P  Silveira H 《Veterinary immunology and immunopathology》2002,88(1-2):21-30

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Pediculosis in Macaca sylvanus of Gibraltar     

Cohn DL  Smith V  Pizarro M  Jones-Engel L  Engel G  Fuentes A  Shaw E  Cortes J 《Veterinary parasitology》2007,145(1-2):116-119

Pedicinus spp. parasitize several species of nonhuman primates. This is the first published report confirming the presence of Pedicinus albidus (Rudow) infestation in the free-ranging macaques (Macaca sylvanus) of Gibraltar. The diagnosis of pediculosis was based upon finding adult lice on host animals.  相似文献   

Composition and spatial organization of fish assemblages in the lower Guadiana basin, southern Iberia   总被引：1，自引：0，他引：1  

F. N. Godiaho  M. T. Ferreira  R. V. Cortes 《Ecology of Freshwater Fish》1997,6(3):134-143

Abstract— A survey of fish assemblages (composition and spatial organization) was made in the lower Guadiana basin (southern Iberia) during the summer of 1994. A total of 6937 specimens belonging to 18 species were collected in 44 channel units and 30 sites (i. c. a series of channel units), comprising all the freshwater species previously recorded in the area except the exotic Esox lucius and the endemic endangered cyprinid Anaecypris hispanica. The endemic cyprinids Chondrostoma lemmingii and Barbus sclateri were rarely encountered, whereas the exotic Lepomis gibbosus was the predominant species found. The lower Guadiana fish community changed in space from assemblages characterized by the high relative abundances of Leuciscus pyrenaicus , smaller size-classes of Barbus spp. and Tropidophoxinellus alburnoides (all Iberian endemisms), to assemblages characterized by the high relative abundances of L. gibbosus, Micropterus salmoides, Cichlasoma facetum (all exotic species) and the larger size-classes of Barbus comiza and Barbus microcephalus . The former assemblages used smaller (i. e., narrow and shallow) sites and channel units, usually in tributaries and closer to headwaters, with smaller L. pyrenaicus being dominant in sites located in the tributaries discharging to the brackish Guadiana. This group was also related to channel units rich in emergent macropthytes. The latter assemblages were related to the largest sites and channel units, typically found in the main river or in tributary sites far from the source. Besides these two groups, some species were associated to noticeable environmental conditions; Blennius fluviatilis to channel units with current velocity and heterogeneous substrate, typically found in the main river, and Cobitis paludica to channel units with heterogeneous substrate.  相似文献