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A commercial broth microdilution system for testing the antimicrobial susceptibility of gram-positive cocci was compared with the standardized disk agar-diffusion method by testing 254 clinical strains of staphylococci and streptococci using both methods. A total of 2,794 parallel determinations were made with 92.3% complete agreement between the 2 methods; of the discrepancies encountered, 3.0% were minor, 2.5% were major, and 2.1% were very major. The results indicate that the commercial microdilution system may provide a reliable quantitative method for antimicrobial susceptibility testing of clinical isolates from animals.  相似文献   
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Biological activity of Glechoma hederacea   总被引:2,自引:0,他引:2  
The n-hexane, dichloromethane and methanol extracts of the aerial parts of Glechoma hederacea have been screened for antibacterial and free radical scavenging activity. General toxicity (brine shrimp lethality assay) of these extracts has also been assessed.  相似文献   
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The level of indole-3-acetic acid (IAA) in shortleaf pine (Pinus echinata Mill.) roots was increased by inoculation with the ectomycorrhizal fungus Pisolithus tinctorius (Pers.) Coker and Couch. Indole-3-acetic acid oxidase activity was also increased suggesting that the increased level of IAA was the result of increased synthesis. Boron fertilization reduced IAA levels in roots inoculated with P. tinctorius but not in noninoculated roots.  相似文献   
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为了探索烟叶生产过程中各机械的性能及工作效率,2013年在福建省建阳市莒口镇河坝新村开展了烟叶生产全程机械化作业试点研究。结果表明,起垄环节,碎土率方面邵武华友履带式起垄机表现最好(89.9%),沟底积土方面松溪永顺表现最好;覆膜环节,各测试机型表现中等,在效率方面优于人工覆膜;移栽环节,漏栽率上江苏久泰移栽机高于江苏井关移栽机;培土环节,漳州南冠培土机操作简便,掉头灵活,单人操作,单行双侧作业,深受烟农喜爱;拔杆环节,龙岩诚德拔杆机单人操作,操作简便,动力较足,拔杆顺畅,拔杆带土较少,效果较好,适应性较强。  相似文献   
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Beds of size-sorted stones forming stripes perpendicular to the contour are conspicuous on hillsides of the Columbia Plateau. Stripes occur on terrain ranging from 0° to about 30° in steepness, often beginning among Mima-type mounds on mesa tops and extending downward onto steep, unmounded slopes. Four mechanisms of their origin have been hypothesized: 1) water erosion, 2) solifluction and soil creep, 3) weathering of rock outcrops, and 4) tunneling by pocket gophers. We measured characteristics of five stripes on slopes of differing exposure and steepness. These stripes were 58–124 m long, and widths showed a maximum range of 0.55–3.70 m. Data on physical and biotic characteristics of the stripes suggest that pocket gopher tunneling is a basic mechanism of stripe formation on gentle slopes, and that this mechanism is augmented by outcrop weathering and colluvial dynamics on steeper slopes, with erosion playing a secondary role.  相似文献   
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To determine influences of insulin and body condition on follicular growth, prepuberal gilts (n = 16) treated with pregnant mare's serum gonadotropin (PMSG) were used in a 2 X 2 factorial experiment with main effects of insulin (0 or .4 IU/kg every 12 h beginning at 1800 on the day before PMSG) and backfat depth (moderate, 25 +/- .8; high, 32 +/- .7 mm; P less than .0001). Body weights were similar. Blood sampling was at 6-h intervals for analyses of LH, FSH, growth hormone (GH), glucagon, cortisol, insulin, insulin-like growth factor-I (IGF-I), plasma urea nitrogen (PUN), nonesterified fatty acids (NEFA), testosterone, estradiol-17 beta, and progesterone. Ovaries were removed 75 h after PMSG treatment, and visible small (less than or equal to 3 mm), medium (4 to 6 mm), large (greater than or equal to 7 mm), and macroscopically atretic follicles were counted. Administration of insulin increased IGF-I in fluid of medium follicles (108.8 vs 60.7 ng/ml; SEM = 13.3; P less than .05). Neither insulin nor fatness affected hCG binding by granulosa cells (12.5 +/- 1.6 ng/10(6) cells) or numbers of large (16.7 +/- 2.6) and medium (10.4 +/- 2.3) follicles. However, insulin increased the number of small follicles (58.9 vs 29.9; SEM = 9.7; P less than .05) and reduced the number of atretic follicles (3.8 vs 11.3; SEM = 1.1; P less than .05). The predominant effect of insulin on reducing number of atretic follicles was in the small size class (.6 vs 6.9; SEM = .6, P less than .01). Follicular fluid estradiol and progesterone were not affected by treatments; however, testosterone concentrations in large follicles were lower in gilts with higher backfat (32.5 vs 59.9 ng/ml; SEM = 4.0; P less than .05). Systemic LH, FSH, glucagon, cortisol, PUN, NEFA, estradiol, and testosterone were not affected by insulin or level of feeding. However, GH was lower in gilts that had higher backfat (overall average of 3.2 vs 2.8 ng/ml; SEM = .1; P less than .05). Insulin reduced atresia and altered intrafollicular IGF-I independently of body condition and without sustained effects on other hormones.  相似文献   
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