Cerebral malacia in a mule with ependymoma          下载免费PDF全文

F. Mendes de Cordova  L. Vaz Burns  A. Tony Ramos  S. Estevan Moron  C. A. Silva de Cordova  G. M. da Luz Silva 《Equine Veterinary Education》2015,27(1):34-38

Ependymoma is a rare tumour of the central nervous system that can vary in anatomical location and produce a broad range of clinical signs. Sporadically described in dogs, cats, cattle and horses, this disease has never previously been reported in a mule. This report describes a case of a 12‐year‐old mule showing neurological signs, including blindness, dysphagia, incoordination, sternal recumbency, depression, apathy and emaciation. Because of the worsening health conditions, the animal was subjected to euthanasia for humane reasons. At necropsy, an area of malacia in the right subcortical region of the frontoparietal area of the cerebrum and an ovoid, firm, grey, 2.4 × 1.0 × 0.7 cm nodule in the right lateral ventricle was observed. Histopathology revealed that the nodule was a densely cellular mass with cells arranged in nests and cords, formations of pseudorosettes with perivascular deposition of fibrillar eosinophilic material and the presence of a few lymphocytes. The cells had rounded and moderately hyperchromatic nuclei, punctate chromatin, distinct nucleoli, and indistinct cytoplasmic margins. Based on the histopathological features, the nodule was diagnosed as an ependymoma, a tumour considered rare, but that may be included in the differential diagnosis for diseases with neurological signs.  相似文献   

Genetic diversity evolution through participatory maize breeding in Portugal   总被引：1，自引：0，他引：1  

Maria Carlota Vaz Patto  Pedro Manuel Moreira  Nuno Almeida  Zlatko Satovic  Silas Pego 《Euphytica》2008,161(1-2):283-291

Natural, and in particular, artificial (human) selection may pose a danger to the existing crop genetic diversity. Nevertheless, on-farm breeding systems seem to achieve phenotypic improvements even though preserving variability. Using SSR markers, we analysed several selection cycles, over a 20 years period, of a Portuguese on-farm participatory maize OPV-‘Pigarro’ breeding project. No significant differences in allelic richness (N_ar), observed heterozygosity (H_O), expected heterozygosity (or gene diversity; H_E) or inbreeding coefficient (f) were detected among the selection cycles. 58 out of 107 alleles were common to all the selection cycles studied. The analysis of molecular variance showed that the variation among selection cycles represented only 7% of the total molecular variation. However, the number of private alleles varied among the selection cycles, being the highest detected at the beginning of the selection project. These findings demonstrate that an allele flow took place during the on-farm selection process of ‘Pigarro’ but the level of genetic diversity was not significantly influenced. Since interesting phenotypic improvements were also achieved, on-farm breeding projects, like this one, should be valued as a way to preserve unique Portuguese maize landraces in risk of disappearing.  相似文献   

Phosphorus availability and grass growth in biochar-modified acid soil: A study excluding the effects of soil pH     

Aymbiré Angeletti Fonseca  Danilo Andrade Santos  Renato Ribeiro Passos  Felipe Vaz Andrade  Otacílio José Passos Rangel 《Soil Use and Management》2020,36(4):714-725

The low efficiency of phosphorus fertilization in weathered soils can limit plant development. The application of biochars in these areas has been seen as an important way to increase the efficiency of phosphorus fertilization and to promote better plant growth. However, biochars are alkaline materials that can increase soil pH and thus change the nutrient dynamics, which has been often ignored in studies of this nature. Here, all treatments had their pH standardized at 6.1 to eliminate the influence of pH on biochar application responses. The main goal of this study was to evaluate the real potential of coffee straw and eucalyptus bark biochars, produced under different pyrolysis temperatures, in the optimization of phosphorus fertilization and the development of Brachiaria brizantha. A greenhouse experiment was set up in a 2 × 2 × 5 factorial scheme, conducted for 120 days. The biochars, prepared from coffee straw and eucalyptus bark at 350 and 600°C, were applied at five rates in a Red-Yellow Oxisol. The application of biochars may reduce the demand for nutrients and correctives, optimize phosphorus fertilization and improve the development of Brachiaria brizantha, but this ability depends on the raw material and the pyrolysis temperature used in its production. All analysed biochars can contribute to higher tillering and dry matter production, but only coffee straw biochars and eucalyptus bark biochar produced at 350°C were efficient in the optimization of phosphorus fertilization until 120 days of cultivation of Brachiaria brizantha.  相似文献   

Modeling and correction of soil penetration resistance for varying soil water content   总被引：1，自引：0，他引：1  

Carlos M.P. Vaz  Juliana M. Manieri  Markus Tuller 《Geoderma》2011,166(1):92-101

For this study penetration resistance (PR) was measured within the profiles of four Oxisols for a wide range of water contents (θ) and bulk densities. Obtained data were utilized to parameterize 23 previously applied regression models. The most promising models were selected to illustrate effects of soil texture on PR. Finally, a new correction method based on normalization of PR with θ corresponding to a matric potential of − 10 kPa was introduced. Evaluation of texture effects revealed that for very wet soils PR was lowest, but increased with clay content. PR at − 1500 kPa exhibited a maximum at clay content of 35% and at − 10 kPa PR was least affected by texture. From all regression models three- and two-parametric exponential and power functions yielded closest matches to measured data. The proposed correction significantly dampened the influence of θ on PR, which allows better comparison for a specific soil or among different soils.  相似文献   

Boophilus microplus cathepsin L-like (BmCL1) cysteine protease: specificity study using a peptide phage display library     

Clara RO  Soares TS  Torquato RJ  Lima CA  Watanabe RO  Barros NM  Carmona AK  Masuda A  Vaz IS  Tanaka AS 《Veterinary parasitology》2011,181(2-4):291-300

The tick Rhipicephalus (Boophilus) microplus is one of the most important bovine ectoparasites, a disease vector responsible for losses in meat and milk productions. A cysteine protease similar to cathepsin L, named BmCL1, was previously identified in R. microplus gut, suggesting a role of the enzyme in meal digestion. In this work, BmCL1 was successfully expressed in Pichia pastoris system, yielding 54.8 mg/L of culture and its activity was analyzed by synthetic substrates and against a R. microplus cysteine protease inhibitor, Bmcystatin. After rBmCl1 biochemical characterization it was used in a selection of a peptide phage library to determine rBmCL1 substrate preference. Obtained sequenced clones showed that rBmCL1 has preference for Leu or Arg at P(1) position. The preference for Leu at position P(1) and the activation of BmCL1 after a Leu amino acid residue suggest possible self activation.  相似文献   

Lead and Helium Isotope Evidence from Oceanic Basalts for a Common Deep Source of Mantle Plumes     

BB Hanan  DW Graham 《Science (New York, N.Y.)》1996,272(5264):991-995

Linear arrays in lead isotope space for mid-ocean ridge basalts (MORBs) converge on a single end-member component that has intermediate lead, strontium, and neodymium isotope ratios compared with the total database for oceanic island basalts (OIBs) and MORBs. The MORB data are consistent with the presence of a common mantle source region for OIBs that is sampled by mantle plumes. 3He/4He ratios for MORBs show both positive and negative correlation with the 206Pb/204Pb ratios, depending on the MORB suite. These data suggest that the common mantle source is located in the transition zone region. This region contains recycled, oceanic crustal protoliths that incorporated some continental lead before their subduction during the past 300 to 2000 million years.  相似文献   

CALCIUM IN PREVENTION AND TREATMENT OF EXPERIMENTAL DDT POISONING     

Vaz Z  Pereira RS  Malheiro DM 《Science (New York, N.Y.)》1945,101(2626):434-436

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Immunohistochemical Localization of Fibroblast Growth Factor‐2 in the Sheep Ovary and its Effects on Pre‐antral Follicle Apoptosis and Development In Vitro     

JMS Santos  VG Menezes  RS Barberino  TJS Macedo  TLB Lins  BB Gouveia  VRP Barros  LP Santos  RJS Gonçalves  MHT Matos 《Reproduction in domestic animals》2014,49(3):522-528

Studies with sheep are important to improve our knowledge about the factors that control folliculogenesis in mammals and to explore possible physiological differences among species. The aims of this study were to characterize FGF‐2 protein expression in ovine ovaries and to verify the effect of FGF‐2 on the morphology, apoptosis and growth of ovine pre‐antral follicles cultured in vitro. After collection, one fragment of ovarian tissue was fixed for histological analysis and TUNEL analysis (fresh control). The remaining fragments were cultured for 7 days in control medium (α‐MEM⁺) alone or supplemented with FGF‐2 at different concentrations (1, 10, 50, 100 or 200 ng/ml). After culturing, ovarian tissue was destined to histology and TUNEL analysis, and oocyte and follicle diameters were measured. The immunostaining for FGF‐2 was observed in oocytes from primordial, primary and secondary follicles, as well as in granulosa cells of secondary and antral follicles. The percentage of normal follicles was similar among control medium, 1 and 10 ng/ml FGF‐2, and significantly higher than those observed in 50, 100 or 200 ng/ml FGF‐2. A significant increase in follicle diameter was observed when tissues were cultured in 10, 50, 100 or 200 ng/ml FGF‐2 compared with the fresh control and the other treatments. Similar results were observed for oocyte diameter in tissues cultured with 50, 100 or 200 ng/ml FGF‐2 (p < 0.05). However, the percentage of apoptotic cells only decreased (p < 0.05) in ovarian tissues cultured in 1 or 10 ng/ml FGF‐2 compared with the control medium and other FGF‐2 treatments. In conclusion, this study demonstrated the presence of FGF‐2 in ovine ovaries. Furthermore, 10 ng/ml FGF‐2 inhibits apoptosis and promotes ovine follicle growth. As the sheep ovary is more similar to that of humans, the culture system demonstrated in this work seems to be an appropriate tool for studies towards human folliculogenesis.  相似文献   

Identification of common genomic regions controlling resistance to <Emphasis Type="Italic">Mycosphaerella pinodes</Emphasis>, earliness and architectural traits in different pea genetic backgrounds     

Sara Fondevilla  Nuno F. Almeida  Zlatko Satovic  Diego Rubiales  Maria C. Vaz Patto  José I. Cubero  Ana M. Torres 《Euphytica》2011,182(1):43-52

In a previous study a genetic map had been developed using a RIL population derived from a cross between the Pisum sativum ssp. syriacum accession P665 and the P. sativum ssp. sativum cv. Messire. This population segregated for several agricultural important traits and was successfully used to identify QTLs (Quantitative Trait Loci) controlling resistance to Mycosphaerella pinodes and Orobanche crenata, earliness, root length and aerial biomass. However, this map contained only a few markers in common with the international pea consensus map, hampering comparison with other pea maps. The objective of this study was to incorporate a set of common transferable and reproducible markers into the P665 × Messire map to favour comparative mapping and QTL validation. Seventy-eight out of the 248 SSRs assayed resulted polymorphic in the parental lines. Thirty-eight of them, uniformly distributed all over the genome, were genotyped in the whole population and included in the map. This SSR enriched map allowed identification of six new QTLs (three for resistance to M. pinodes, two for resistance to broomrape and one for root length). Inclusion of the SSRs confirmed the homology of some of the QTLs identified in the population P665 × Messire with other QTLs associated with related traits in different pea genetic backgrounds.  相似文献   

Days in milk alters the milk fatty acid profile of grazing donkeys: A preliminary study     

Madian Johel Galo Salgado  Aline Rocha Silva  Carolina Oliveira de Souza  Paulo Vitor França Lemos  Ronaldo Lopes Oliveira  Chiara Albano de Araújo Oliveira  Claudio Vaz Di Mambro Ribeiro 《Journal of animal physiology and animal nutrition》2021,105(6):1173-1178

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