Contents of carboxylic acids and two phenolics and antioxidant activity of dried portuguese wild edible mushrooms     

Ribeiro B  Rangel J  Valentão P  Baptista P  Seabra RM  Andrade PB 《Journal of agricultural and food chemistry》2006,54(22):8530-8537

The organic acids and phenolics compositions of nine wild edible mushrooms species (Suillus bellini, Tricholomopsis rutilans, Hygrophorus agathosmus, Amanita rubescens, Russula cyanoxantha, Boletus edulis, Tricholoma equestre, Suillus luteus, and Suillus granulatus) were determined by HPLC-UV and HPLC-DAD, respectively. The antioxidant potential of these species was also assessed by using the DPPH* scavenging assay. The results showed that all of the species presented a profile composed of at least five organic acids: oxalic, citric, malic, quinic, and fumaric acids. In a general way, the pair of malic plus quinic acids were the major compounds. Only very small amounts of two phenolic compounds were found in some of the analyzed species: p-hydroxybenzoic acid (in A. rubescens, R. cyanoxantha, and T. equestre) and quercetin (in S. luteus and S. granulatus). All of the species exhibited a concentration-dependent scavenging ability against DPPH*. T. rutilans revealed the highest antioxidant capacity.  相似文献   

Modeling the Potential Geographic Distribution of Black Pepper（Piper nigrum）in Asia Using GIS Tools     

HAO Chao-yun  FAN Rui  Milton Cezar Ribeiro  TAN Le-he  WU Hua-song  YANG Jian-feng  ZHENG Wei-quan  YU Huan 《中国农业科学(英文版)》2012,11(4):593-599

Known as the "king of spices",black pepper (Piper nigrum),a perennial crop of the tropics,is economically the most important and the most widely used spice crop in the world.To understand its suitable ...  相似文献   

The thermostable β-1,3-1,4-glucanase from Clostridium thermocellum improves the nutritive value of highly viscous barley-based diets for broilers     

Ribeiro T  Lordelo MM  Prates JA  Falcão L  Freire JP  Ferreira LM  Fontes CM 《British poultry science》2012,53(2):224-234

1. Microbial β-1,3-1,4-glucanases improve the nutritive value of barley-based diets for poultry by effectively decreasing the degree of polymerisation of the anti-nutritive soluble β-glucans. Glycoside hydrolases (GHs) acting on recalcitrant polysaccharides display a modular architecture comprising a catalytic domain linked to one or more non-catalytic Carbohydrate-Binding Modules (CBMs).

2. GHs and CBMs have been classified in different families based on primary structure similarity (see CAZy webpage at http://www.cazy.org). The role of CBMs is to anchor the appended GHs into their target substrates, therefore eliciting the efficient hydrolysis of structural carbohydrates.

3. Here we describe the biochemical properties of the family 16 GH from Clostridium thermocellum, termed CtGlc16A. CtGlc16A is a thermostable enzyme that specifically acts on β-1,3-1,4-glucans with a remarkable catalytic activity (38000?U/mg protein).

4. CtGlc16A, individually or fused to the family 11 β-glucan-binding domain of cellulase CtLic26A-Cel5E of C. thermocellum, was used to supplement a highly viscous barley-based diet for broilers.

5. The data showed that birds fed on diets supplemented with the recombinant enzymes displayed an improved performance when compared with birds given diets not supplemented with exogenous enzymes. However, inclusion of the non-catalytic CBMs had no influence on the capacity of CtGlc16A to reduce the anti-nutritive effects of soluble β-1,3-1,4-glucans.

6. The data suggest that at elevated dosage rates, CBMs might be unable to potentiate the catalytic activity of appended catalytic domains; this effect may only be revealed when feed enzymes are incorporated at low levels.  相似文献   

Use of a Real Time PCR for detecting subspecies of Babesia canis     

Costa LM  Zahler-Rinder M  Ribeiro MF  Rembeck K  Rabelo EM  Pfister K  Passos LM 《Veterinary parasitology》2012,188(1-2):160-163

This paper reports the development and use of a Real Time PCR for detection of Babesia canis canis, B. canis rossi, and B. canis vogeli in endemic areas of Brazil. The sequences of the internal transcribed spacer (ITS) of several organisms were aligned and five primers and four probes were designed for amplification of a fragment (around 125 bp) which differentiates subspecies of B. canis. Blood samples collected from dogs living in farms in three distinct rural regions within the State of Minas Gerais (Lavras, Belo Horizonte and Nanuque) were tested. Blood samples had been collected during a dry season (Lavras, n=100; Belo Horizonte, n=50; Nanuque, n=102); the dogs were re-sampled in the subsequent rainy season (Lavras, n=71; Belo Horizonte, n=29; Nanuque, n=66). From each sample, DNA was extracted and Giemsa stained smears were microscopically examined for direct detection of Babesia parasites. B. canis vogeli was the only subspecies found, with an overall prevalence of 9.9% during the dry season and 10.8% during the rainy season. Dogs living in Nanuque and Belo Horizonte showed significantly higher prevalence rates than those living in Lavras (13.7%, 12.0% and 5.0%, respectively). The Real Time PCR developed proved to be appropriate to detect B. canis subspecies in endemic areas.  相似文献   

Diversity of non‐structural carbohydrates in grasses (Poaceae) from Brazil     

M. G. Moraes  N. J. Chatterton  P. A. Harrison  T. S. Filgueiras  R. C. L. Figueiredo‐Ribeiro 《Grass and Forage Science》2013,68(1):165-177

Non‐structural carbohydrates (NSC) are the primary energy available for growth and dry‐mass production in forage grasses. They are also associated with tolerance to environmental stresses, including drought. Soluble carbohydrates, especially fructans of temperate forage grasses, have been extensively studied; however, little is known about NSC of tropical grasses. These plants are abundant in the Cerrado, a savanna‐like vegetation with a definite seasonality in rainfall distribution. Such an environment presents an ideal condition for the occurrence of fructans. However, the present analysis of carbohydrates in aerial parts of twenty‐four tropical grass species, mainly from the Panicoideae subfamily in the Cerrado, shows a distinctively different NSC profile when compared with temperate grasses. Free glucose and starch are common NSC found in most plant organs. However, free fructose and sucrose‐based oligosaccharides were only found in very low amounts. Sucrose was detected in higher amounts only in stems. A linear series of malto‐oligosaccharides was found in leaf blades, sheaths and stems of most of the Cerrado grasses, except for those from the Andropogoneae tribe. These results indicate a diversity of regulatory pathways of NSC metabolism in tropical forage grasses from the Cerrado.  相似文献   

Retrospective analysis of complications and outcomes in Boxers and Staffordshire Bull Terriers undergoing cranial cruciate ligament surgery     

AS Levien  DC Brodbelt  GI Arthurs 《Australian veterinary journal》2013,91(6):220-225

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Pathogenesis of reproductive failure induced by Trypanosoma vivax in experimentally infected pregnant ewes     

Taciana MF Silva  Roberio G Olinda  Carla MF Rodrigues  Ant?nio CL Camara  Francisco C Lopes  Wesley AC Coelho  Múcio FB Ribeiro  Carlos IA Freitas  Marta MG Teixeira  Jael S Batista 《Veterinary research》2013,44(1):1

The present study was aimed at investigating the effect of experimental infection by Trypanosoma vivax in different stages of pregnancy, determining the pathogenesis of reproductive failure, and confirming transplacental transmission. We used 12 pregnant ewes distributed into four experimental groups: G1, was formed by three ewes infected with T. vivax in the first third of pregnancy (30 days); G2 comprised three infected ewes in the final third of pregnancy (100 days); G3 and G4 were composed of three non-infected ewes with the same gestational period, respectively. Each ewe of G1 and G2 was inoculated with 1.25 × 10⁵ tripomastigotes. Clinical examination, determination of parasitemia, serum biochemistry (albumin, total protein, glucose, cholesterol, and urea), packed cell volume (PCV), serum progesterone, and pathological examination were performed. Placenta, amniotic fluid, blood and tissues from the fetuses and stillbirths were submitted to PCR. Two ewes of G1 (Ewe 1 and 3) presented severe infection and died in the 34^th and 35^th days post-infection (dpi), respectively; but both fetuses were recovered during necropsy. In G2, Ewe 5 aborted two fetuses on the 130^th day (30 dpi) of pregnancy; and Ewe 6 aborted one fetus in the 140^th day (40 dpi) of gestation. Ewes 2 and 4 delivered two weak lambs that died five days after birth. Factors possibly involved with the reproductive failure included high parasitemia, fever, low PCV, body score, serum glucose, total protein, cholesterol, and progesterone. Hepatitis, pericarditis, and encephalitis were observed in the aborted fetuses. The presence of T. vivax DNA in the placenta, amniotic fluid, blood, and tissues from the fetuses confirms the transplacental transmission of the parasite. Histological lesion in the fetuses and placenta also suggest the involvement of the parasite in the etiopathogenesis of reproductive failure in ewes.  相似文献   

Extracts of Peels and Seeds of Five Varieties of Brazilian Jabuticaba Present High Capacity to Deactivate Reactive Species of Oxygen and Nitrogen     

Paludo  Michelly Cristiane  de Oliveira  Luciana Fontes  Hermosín-Gutiérrez  Isidro  Ballus  Cristiano Augusto  Ribeiro  Alessandra Braga  de Oliveira  Silvia Borges Pimentel  Godoy  Helena Teixeira 《Plant foods for human nutrition (Dordrecht, Netherlands)》2019,74(1):135-140

Plant Foods for Human Nutrition - Jabuticaba has a high concentration of phenolic compounds, which have a significant antioxidant capacity. Methodologies have been developed to evaluate the ability...  相似文献   

Effect of Freeze-Dried Red Beet (Beta vulgaris L.) Leaf Supplementation on Biochemical and Anthropometrical Parameters in Overweight and Obese Individuals: a Pilot Study     

de Castro  Ana Paula Ribeiro Barcelos  da Cunha  Diogo Thimoteo  Antunes  Adriane Elisabete Costa  Corona  Ligiana Pires  Bezerra  Rosângela Maria Neves 《Plant foods for human nutrition (Dordrecht, Netherlands)》2019,74(2):232-234

Plant Foods for Human Nutrition - Obesity is an important public health concern and is directly related with chronic noncommunicable diseases that affect the health of millions of people around the...  相似文献   

Yerba Maté (Ilex paraguariensis) aqueous extract decreases intestinal SGLT1 gene expression but does not affect other biochemical parameters in alloxan-diabetic Wistar rats     

Oliveira DM  Freitas HS  Souza MF  Arçari DP  Ribeiro ML  Carvalho PO  Bastos DH 《Journal of agricultural and food chemistry》2008,56(22):10527-10532

Yerba maté (Ilex paraguariensis) is rich in polyphenols, especially chlorogenic acids. Evidence suggests that dietary polyphenols could play a role in glucose absorption and metabolism. The aim of this study was to evaluate the antidiabetic properties of yerba maté extract in alloxan-induced diabetic Wistar rats. Animals (n = 41) were divided in four groups: nondiabetic control (NDC, n = 10), nondiabetic yerba maté (NDY, n = 10), diabetic control (DC, n = 11), and diabetic yerba maté (DY, n = 10). The intervention consisted in the administration of yerba maté extract in a 1 g extract/kg body weight dose for 28 days; controls received saline solution only. There were no significant differences in serum glucose, insulin, and hepatic glucose-6-phosphatase activity between the groups that ingested yerba maté extract (NDY and DY) and the controls (NDC and DC). However, the intestinal SGLT1 gene expression was significantly lower in animals that received yerba maté both in upper (p = 0.007) and middle (p < 0.001) small intestine. These results indicate that bioactive compounds present in yerba maté might be capable of interfering in glucose absorption, by decreasing SGLT1 expression.  相似文献