Identification and quantification of benzimidazole resistance polymorphisms in Haemonchus contortus isolated in Northeastern Brazil     

Jessica Maria Leite dos Santos  Jomar Patrício Monteiro  Wesley Lyeverton Correia Ribeiro  Iara Tersia Freitas Macedo  Ana Lourdes Fernandes Camurça-Vasconcelos  Luiz da Silva Vieira  Claudia Maria Leal Bevilaqua 《Veterinary parasitology》2014,199(3-4):160-164

Haemonchus contortus is the most prevalent nematode in Brazil. The objective of this study was to select 6 populations of H. contortus of known or suspected benzimidazole resistance status and characterize these using quantitative real-time polymerase chain reaction (qPCR) for single nucleotide polymorphisms (SNPs) F200Y, F167Y and E198A in the β-tubulin isotype 1 gene. qPCR was performed using DNA from a pool of 10 adult male H. contortus from a single animal per farm. Faecal egg count reduction test (FECRT) and egg hatch test (EHT) were used to determine the resistance status. Samples were obtained from 6 farms located in 5 counties in the Ceará State: Tauá, Boa Viagem, Quixadá, Santa Quitéria and Solonópole. The inbred-susceptible-Edinburgh (ISE) isolate was used as reference for comparative purposes in the qPCR. Benzimidazole resistance was detected by FECRT on all farms with efficacy values ranging from 0 to 51%. EC50 values as determined by EHT were all above 1.49 μg/ml. High frequencies of the resistant SNPs F200Y and F167Y alleles were detected but no resistance was detected at SNP E198A. Our results suggest that the SNPs F167Y and F200Y are both important for benzimidazole resistance in the studied populations.  相似文献   

Strangulation of the small intestine by an anomalous congenital band in a yearling     

A. C. O. Dearo  G. de Moraes Marcondes  J. C. O. Araújo  C. E. S. Grumadas  P. C. Marino  W. T. Teixeira  G. de La Penha Chiacchio Fernandes 《Equine Veterinary Education》2014,26(12):640-644

Strangulating obstruction of the small intestine by anomalous mesenteric bands is an uncommon cause of colic in horses. The most commonly reported bands are mesodiverticular bands that are embryological remnants of the vitelline arteries. Despite the congenital nature of the anomaly, a wide age range of horses can be affected. This report describes a case of small intestinal strangulating obstruction caused by an unusual, anomalous congenital band attached to the ileocaecal fold in a yearling colt. Information including case background, history, clinical, laboratory, surgical and pathological findings is described.  相似文献   

Hydrology-oriented (adaptive) silviculture in a semiarid pine plantation: How much can be modified the water cycle through forest management?     

Antonio D. del Campo  Tarcísio J. G. Fernandes  Antonio J. Molina 《European Journal of Forest Research》2014,133(5):879-894

Hydrology-oriented silviculture might adapt Mediterranean forests to climatic changes, although its implementation demands a better understanding and quantification on the water fluxes. The influence of thinning intensity (high, medium, low and a control) and its effect on the mid-term (thinned plots in 1998 and 2008) on the water cycle (transpiration, soil water and interception) and growth [basal area increment (BAI)] were investigated in 55-year-old Aleppo pine trees. Thinning enhanced a lower dependence of growth on climate fluctuations. The high-intensity treatment showed significant increases in the mean annual BAI (from 4.1 to 17.3 cm²) that was maintained in the mid-term. Thinning intensity progressively increased the sap flow velocity (v _s) in all cases with respect to the control. In the mid-term, an increased functionality of the inner sapwood was also observed. Mean daily tree water use ranged from 5 (control) to 18 (high intensity) l tree^?1. However, when expressed on an area basis, daily transpiration ranged from 0.18 (medium) to 0.30 mm (control), meaning that in spite of the higher transpiration rates in the remaining trees, stand transpiration was reduced with thinning. Deep infiltration of water was also enhanced with thinning (about 30 % of rainfall) and did not compete with transpiration, as both presented opposite seasonal patterns. The changes in the stand water relationships after 10 years were well explained by the forest cover metric. The blue to green water ratio changed from 0.15 in the control to 0.72 in the high-intensity treatment, with the remaining treatments in the 0.34–0.48 range.  相似文献   

Dissolved nutrient release from solid wastes of southern bluefin tuna (Thunnus maccoyii, Castelnau) aquaculture     

Milena Fernandes  Michael Angove  Talya Sedawie  & Anthony Cheshire 《Aquaculture Research》2007,38(4):388-397

Finfish pens are point sources of dissolved nutrients released from fish metabolism or degradation of solid wastes. Nutrients leaching from uneaten feed and faeces are not usually quantified in mass budgets for these systems, leading to an overestimation of fish retention or deposition to the seabed. In this study, we investigated nutrient leaching from pellets and baitfish feed as well as faeces of southern bluefin tuna (Thunnus maccoyii) into seawater. Faeces were nitrogen depleted (51–54 mg N g⁻¹ dw) and phosphorus enriched (62–72 mg P g⁻¹ dw) compared with feeds (83–111 mg N g⁻¹ dw and 17–21 mg P g⁻¹ dw). Less phosphorus was available for leaching from pellets and faeces of pellet‐fed tuna (5–6%) than from baitfish and faeces of baitfish‐fed tuna (17–21%). The proportion of soluble nitrogen in pellets (15%) was also lower than in baitfish and faeces (35–43%). Leaching loads for a feed conversion ratio of 5 were estimated as 22 and 26 kg N tonne⁻¹ growth when baitfish or pellets were used as feed respectively. Phosphorus loads were estimated as 15 and 4 kg P tonne⁻¹ growth respectively. More than 90% of nitrogen loads, and approximately 50% of phosphorus, are likely to be released into seawater before solid wastes reach the seafloor.  相似文献   

Environmental impacts of aquaculture (Sheffield Biological Sciences,Volume 5), by Black K.D. 2001, 219 pp. Sheffield Academic Press,Sheffield, UK,ISBN 1–84127–041–5.     

Teresa Fernandes 《水产资源保护:海洋与淡水生态系统》2003,13(1):91-92

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Microsatellite variability in natural populations of the blackspot seabream Pagellus bogaraveo (Brünnick, 1768): a database to access parentage assignment in aquaculture     

Andreia Lemos  Ana Isabel Freitas  Ana Teresa Fernandes  Rita Gonçalves  José Jesus  Carlos Andrade  & António Brehm 《Aquaculture Research》2006,37(10):1028-1033

Eight microsatellite loci previously reported were assessed for their utility in parentage assignment in 96 individuals belonging to natural populations of the blackspot seabream Pagellus bogaraveo (Brünnick, 1768) from the Mediterranean and Northeast Atlantic regions. At the mtDNA level, no differentiation was found between these two regions but based on microsatellite data an overall discrete genetic differentiation is perceivable between the two regions separated by the Strait of Gibraltar. The number of alleles per locus ranged from 8 to 30. A database constructed with allele frequency data from six populations was used in a simulation parentage assignment test using the software cervus . The test showed that the number of markers used is enough to perform parentage assignments with real data. The polymorphic information content for each locus was very high (mean value of 0.849), with a total exclusionary power of 0.9995. In summary, seven of the eight microsatellites analysed proved to be sufficient and powerful tools for parentage assignment in hatcheries and the allele frequency data given here can be used to perform pedigree analysis against which real data may be tested.  相似文献   

An investigation of avoidance by Antarctic krill of RRS James Clark Ross using the Autosub-2 autonomous underwater vehicle     

Andrew S. Brierley  Paul G. Fernandes  Mark A. Brandon  Frederick Armstrong  Nicholas W. Millard  Steven D. McPhail  Peter Stevenson  Miles Pebody  James Perrett  Mark Squires  Douglas G. Bone  Gwyn Griffiths 《Fisheries Research》2003,60(2-3):569-576

The autonomous underwater vehicle (AUV) Autosub-2 was deployed on eight missions ahead of RRS James Clark Ross in the northern Weddell Sea and in the Bransfield Strait, Southern Ocean, to assess avoidance of the research vessel by Antarctic krill Euphausia superba. The AUV was equipped with the same type of scientific echosounder as the research vessel (Simrad EK500 operating at 38 and 120 kHz) and measured the density of krill along transect acoustically (g m⁻² wet mass) prior to the ship’s arrival. We hypothesised that if krill avoided the ship, perhaps in response to radiated noise, then the ship should detect less krill than the AUV which is known to have much lower noise levels than the ship. We were unable to detect any significant difference between the density of krill detected by the ship or the AUV, either at the transect level or at finer scales within transects. We conclude, therefore, that avoidance by krill of RRS James Clark Ross will not significantly bias acoustic estimates of krill abundance by this vessel.  相似文献   

Treatment of rupture of suspensory ligament and superficial flexor tendon in a bull     

DR NELSON  SK KNELLER  DD MARTIN 《Australian veterinary journal》1997,75(4):244-246

Rupture of the suspensory ligament at the insertions on the proximal sesamoid bones, and of the superficial flexor tendon of the left fore limb, occurred in an adult Angus bull as a result of fighting. There was severe hyperextension of the metacarpophalangeal (MCP) joint with the dewclaws almost touching the ground. Radiographs revealed severe hyper-extension of the MCP joint with the sesamoid bones aligned directly distal to the metacarpus. Initially, a full length fiberglass cast was applied with the limb partially flexed within the cast and the heels elevated. The cast was replaced twice. The cast was removed after 136 days and the bull was bearing full weight on the limb. Prolonged immobilisation of the limb produced new bone in the area (a normal response in cattle) to cause ankylosis of the traumatized MCP joint and partial ankylosis of the carpus. The bull was being used for pasture breeding one year after the injury.  相似文献   

Mechanical comparison of materials used for extra-capsular stabilisation of the stifle joint in dogs     

DD LEWIS  BK MILTHORPE  CR BELLENGER 《Australian veterinary journal》1997,75(12):890-896

Objective The mechanical properties of three materials (No. 2 polypropylene, No. 5 polybutilate-coated multifilament polyester and 18, 27 and 36 kg test monofilament nylon leader material) commonly used for extra-capsular stabilisation of the stifle in dogs with cranial cruciate ligament insufficiency were determined. The ability of No. 5 polybutilate-coated multifilament polyester and 36 kg test monofilament nylon leader material, when placed as extra-capsular sutures, to mitigate cranial drawer was evaluated in hindlimbs of cadavers. Design An in vitro mechanical study. Animals Seven pairs of hindlimbs harvested from adult greyhound dogs recently euthanased for other reasons. Procedure Samples of each material, including samples of 27 kg test leader material that had been sterilised by one of three methods (ethylene oxide, one or five cycles in an autoclave), were loaded to determine tensile and stress relaxation properties. The effect of cyclic loading on a No. 5 polybutilate-coated multifilament polyester and 36 kg test leader material was also determined. Using the harvested hindlimbs, cranial drawer was measured before and after transection of the cranial cruciate ligament and on the first and twelfth cycle following extra-capsular stabilisation with either No. 5 polybu-tilate-coated multifilament suture or 36 kg test leader material. Results Leader material was found to have the most suitable mechanical characteristics for use as extracapsular stabilisation of the cranial cruciate ligament deficient stifle. Of the sterilisation methods, ethylene oxide was found to have the least detrimental effects on the handling and material characteristics of the leader material. Stifles stabilised with 36 kg test leader material had significantly less drawer than those stabilised with No. 5 polybutilate-coated multifilament polyester suture. Clinical implications Monofilament nylon leader material would appear to have suitable mechanical properties for extra-capsular stabilisation of the cranial cruciate ligament deficient stifle. If possible the material should be sterilised using ethylene oxide.  相似文献   

Energy and protein requirements for maintenance and growth of Boer crossbred kids   总被引：7，自引：0，他引：7  

Fernandes MH  Resende KT  Tedeschi LO  Fernandes JS  Silva HM  Carstens GE  Berchielli TT  Teixeira IA  Akinaga L 《Journal of animal science》2007,85(4):1014-1023

Meat production by goats has become an important livestock enterprise in several parts of the world. Nonetheless, energy and protein requirements of meat goats have not been defined thoroughly. The objective of this study was to determine the energy and protein requirements for maintenance and growth of 34 (3/4) Boer x (1/4) Saanen crossbred, intact male kids (20.5 +/- 0.24 kg of initial BW). The baseline group was 7 randomly selected kids, averaging 21.2 +/- 0.36 kg of BW. An intermediate group consisted of 6 randomly selected kids, fed for ad libitum intake, that were slaughtered when they reached an average BW of 28.2 +/- 0.39 kg. The remaining kids (n = 21) were allocated randomly on d 0 to 3 levels of DMI (treatments were ad libitum or restricted to 70 or 40% of the ad libitum intake) within 7 slaughter groups. A slaughter group contained 1 kid from each treatment, and kids were slaughtered when the ad libitum treatment kid reached 35 kg of BW. Individual body components (head plus feet, hide, internal organs plus blood, and carcass) were weighed, ground, mixed, and subsampled for chemical analyses. Initial body composition was determined using equations developed from the composition of the baseline kids. The calculated daily maintenance requirement for NE was 77.3 +/- 1.05 kcal/kg(0.75) of empty BW (EBW) or 67.4 +/- 1.04 kcal/kg(0.75) of shrunk BW. The daily ME requirement for maintenance (118.1 kcal/kg(0.75) of EBW or 103.0 kcal/kg(0.75) of shrunk BW) was calculated by iteration, assuming that the heat produced was equal to the ME intake at maintenance. The partial efficiency of use of ME for NE below maintenance was 0.65. A value of 2.44 +/- 0.4 g of net protein/kg(0.75) of EBW for daily maintenance was determined. Net energy requirements for growth ranged from 2.55 to 3.0 Mcal/kg of EBW gain at 20 and 35 kg of BW, and net protein requirements for growth ranged from 178.8 to 185.2 g/kg of EBW gain. These results suggest that NE and net protein requirements for growing meat goats exceed the requirements previously published for dairy goats. Moreover, results from this study suggest that the N requirement for maintenance for growing goats is greater than the established recommendations.  相似文献