全文获取类型
收费全文 | 461篇 |
免费 | 57篇 |
专业分类
林业 | 53篇 |
农学 | 9篇 |
89篇 | |
综合类 | 17篇 |
农作物 | 24篇 |
水产渔业 | 75篇 |
畜牧兽医 | 193篇 |
园艺 | 8篇 |
植物保护 | 50篇 |
出版年
2023年 | 5篇 |
2022年 | 9篇 |
2021年 | 22篇 |
2020年 | 28篇 |
2019年 | 28篇 |
2018年 | 35篇 |
2017年 | 33篇 |
2016年 | 24篇 |
2015年 | 23篇 |
2014年 | 21篇 |
2013年 | 36篇 |
2012年 | 31篇 |
2011年 | 36篇 |
2010年 | 13篇 |
2009年 | 24篇 |
2008年 | 17篇 |
2007年 | 22篇 |
2006年 | 18篇 |
2005年 | 7篇 |
2004年 | 19篇 |
2003年 | 14篇 |
2002年 | 12篇 |
2001年 | 6篇 |
2000年 | 4篇 |
1999年 | 3篇 |
1998年 | 4篇 |
1997年 | 5篇 |
1996年 | 1篇 |
1992年 | 2篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1987年 | 3篇 |
1986年 | 1篇 |
1985年 | 4篇 |
1974年 | 2篇 |
1972年 | 1篇 |
排序方式: 共有518条查询结果,搜索用时 296 毫秒
51.
Claus MP Lunardi M Alfieri AF Ferracin LM Fungaro MH Alfieri AA 《Veterinary microbiology》2008,132(3-4):396-401
The amplification by degenerate primers FAP59/FAP64 and sequencing allowed the detection of 15 putative new BPV types in cutaneous warts as well as in healthy skin. Four of these isolates were recently recognized as new BPV types (BPV-7, -8, -9, and -10) after determination of their complete genome sequences. In Brazil, investigations involving the definition of BPV types present in skin warts are still rare. The aim of the current study was to identify the BPV types associated with cutaneous papillomatosis observed in Brazilian cattle herds. Twenty-two cutaneous papilloma specimens were submitted to PCR assay employing the FAP primer pair. All PCR products with approximately 480 bp were submitted to direct sequencing. Cloning was performed for the amplicons which prior analysis revealed as putative new BPV types. From 16 cutaneous lesions, BPV-1, -2, and -6 were identified in two, six, and eight papilloma specimens, respectively. In addition, four putative new BPV types were identified in other six skin warts, and then designated as BPV/BR-UEL2 to -5. The detection of the BPV-1, -2, and -6 types in skin wart specimens supports the existence of these BPV types throughout the Brazilian cattle herd. In addition, the identification of four putative new BPV types is the first report of the presence of different BPV types in the American continent. 相似文献
52.
Rall VL Vieira FP Rall R Vieitis RL Fernandes A Candeias JM Cardoso KF Araújo JP 《Veterinary microbiology》2008,132(3-4):408-413
Milk is considered a nutritious food because it contains several important nutrients including proteins and vitamins. Conversely, it can be a vehicle for several pathogenic bacteria such as Staphylococcus aureus. This study aimed to analyze the frequency of genes encoding the staphylococcal enterotoxins (SEs) SEA, SEB, SEC, SED, SEE, SEG, SEH, SEI and SEJ in S. aureus strains isolated from raw or pasteurized bovine milk. S. aureus was found in 38 (70.4%) out of 54 raw milk samples at concentrations of up to 8.9 x 10(5) CFU/ml. This microorganism was present in eight samples of pasteurized milk before the expiration date and in 11 samples analyzed on the expiration date. Of the 57 strains studied, 68.4% were positive for one or more genes encoding the enterotoxins, and 12 different genotypes were identified. The gene coding for enterotoxin A, sea, was the most frequent (16 strains, 41%), followed by sec (8 strains, 20.5%), sed (5 strains, 12.8%), seb (3 strains, 7.7%) and see (2 strains, 5.1%). Among the genes encoding the other enterotoxins, seg was the most frequently observed (11 strains, 28.2%), followed by sei (10 strains) and seh and sej (3 strains each). With the recent identification of new SEs, the perceived frequency of enterotoxigenic strains has increased, suggesting that the pathogenic potential of staphylococci may be higher than previously thought; however, further studies are required to assess the expression of these new SEs by S. aureus, and their impact in foodborne disease. The quality of Brazilian milk is still low, and efforts from the government and the entire productive chain are required to attain consumer safety. 相似文献
53.
Nunes EA Bonatto SJ de Oliveira HH Rivera NL Maiorka A Krabbe EL Tanhoffer RA Fernandes LC 《Research in veterinary science》2008,84(1):62-67
Conjugated linoleic acids (CLA), 9-cis:11-trans and 10-trans:12-cis, have been shown to be able to modify some immune cells parameters and plasma lipids in a variety of experiment models. Since lymphocytes and polymorphonuclear cells (PMNC) have a large spectrum functions in the immune response, the knowledge in this field has to be expanded. Beagle dogs were fed a control diet or a CLA supplemented diet for nine months. Blood was collected for biochemical analysis and lymphocyte and PMNC isolation. PMNC were assayed for lysosome content, phagocytic activity and superoxide anion production. A lymphocyte proliferation capacity assay was done. The CLA fed dogs had a 34% reduction in total cholesterol (P < 0.05), 28% in LDL (P < 0.05) and 28% non-HDL-cholesterol (P < 0.05). Neither of the PMNC parameters evaluated demonstrated significant alteration. Lymphocytes from CLA group increased by 45% their mitotic capacity (P < 0.05). Our study demonstrates that CLA can successfully modify the lipid profile of dogs (monogastrics) when fed at reasonable levels, but did not significantly alter inflammatory function as would generally predicted. Further, we had some indication that CLA modulated T cell responsiveness. 相似文献
54.
Rhaony Gonalves Leite Elider Prates Romanzini Lutti Maneck Delevatti Alvair Hoffmann Adriana Cristina Ferrari Andr Pastori D'Aurea Lauriston Bertelli Fernandes Amanda Prates Oliveira Ricardo Andrade Reis 《Animal Science Journal》2019,90(5):628-636
Organic additives are recently being used in animal diets owing to their ability to control metabolic issues and result in better animal performance. Specifically, the organic additive Fator P® presents an additional advantage that is to cause a lesser greenhouse gas emission. This study evaluated whether Fator P® intake changes ruminal parameters or animal performance of beef cattle. Evaluations were carried out in a feedlot experiment divided into growing (46 days; two diets [control mix—CM and standard mix—SM] and finishing (lasted 83 days; four diets: CM, SM, Fator P® + virginiamycin, and Fator P® alone [FP]) trials. Animal performance study involved 48 animals allocated to 12 collective pens in completely randomized experimental design. Ruminal parameters were evaluated in separate metabolism study developed carried out using individual pen with four steers. During growing trial, FP diet resulted in higher (p < 0.05) dry matter intake (DMI) and ruminating time. In the finishing trial, diets containing Fator P® resulted in higher DMI than obtained with CM. Most of the ruminal parameters did not differ (p > 0.05) among dietary treatments. Therefore, Fator P® represents a viable and safe strategy for supplementation to beef cattle finished using high‐concentrate diet in feedlot systems. 相似文献
55.
Daphne W. Goldberg Miriam R. Fernandes Fbio P. Sellera Daniela G. C. Costa Ana Paula Loureiro Bracarense Nilton Lincopan 《Zoonoses and public health》2019,66(5):540-545
CTX‐M‐type extended‐spectrum β‐lactamase (ESBL)‐producing Enterobacteriaceae have become identified in marine ecosystem constituting a serious ecological issue. In this respect, although contamination of coastal waters and seafood, and even colonization of seabirds and fishes have been increasingly reported, molecular data are lacking to elucidate the clinical impact of ESBL producers in infected marine animals. In this study, using a genomic approach, we have analysed the genetic background of CTX‐M‐15‐producing Enterobacter hormaechei (belonging to the international human clone ST114) and Citrobacter freundii (ST265) co‐infecting a free‐living green turtle (Chelonia mydas) suffering from septic arthritis, which progressed to generalized coelomitis and death. Wide resistome of these pathogens contributed to treatment failure and death of the animal. 相似文献
56.
Araújo A. R. Rodriguez N. M. Rogério Marcos Cláudio Pinheiro Borges I. Saliba E. O. S. Santos S. A. Pompeu R. C. F. F. Fernandes F. E. P. Monteiro J. P. Muir J. P. 《Tropical animal health and production》2019,51(4):957-966
Tropical Animal Health and Production - Sheep production systems in Brazilian caatinga rangelands require supplementation adapted to changes in floristic and chemical composition as dry seasons... 相似文献
57.
Genetic diversity,anti‐microbial resistance,plasmid profile and frequency of the Vi antigen in Salmonella Dublin strains isolated in Brazil 下载免费PDF全文
F. P. Vilela M. R. Frazão D. P. Rodrigues R. G. Costa M. R. T. Casas S. A. Fernandes J. P. Falcão F. Campioni 《Zoonoses and public health》2018,65(1):e34-e43
Salmonella Dublin is strongly adapted to cattle causing enteritis and/or systemic disease with high rates of mortality. However, it can be sporadically isolated from humans, usually causing serious disease, especially in patients with underlying chronic diseases. The aim of this study was to molecularly type S. Dublin strains isolated from humans and animals in Brazil to verify the diversity of these strains as well as to ascertain possible differences between strains isolated from humans and animals. Moreover, the presence of the capsular antigen Vi and the plasmid profile was characterized in addition to the anti‐microbial resistance against 15 drugs. For this reason, 113 S. Dublin strains isolated between 1983 and 2016 from humans (83) and animals (30) in Brazil were typed by PFGE and MLVA. The presence of the capsular antigen Vi was verified by PCR, and the phenotypic expression of the capsular antigen was determined serologically. Also, a plasmid analysis for each strain was carried out. The strains studied were divided into 35 different PFGE types and 89 MLVA‐types with a similarity of ≥80% and ≥17.5%, respectively. The plasmid sizes found ranged from 2 to >150 kb and none of the strains studied presented the capsular antigen Vi. Resistance or intermediate resistance was found in 23 strains (20.3%) that were resistant to ampicillin, ciprofloxacin, chloramphenicol, imipenem, nalidixic acid, piperacillin, streptomycin and/or tetracycline. The majority of the S. Dublin strains studied and isolated over a 33‐year period may descend from a common subtype that has been contaminating humans and animals in Brazil and able to cause invasive disease even in the absence of the capsular antigen. The higher diversity of resistance phenotypes in human isolates, as compared with animal strains, may be a reflection of the different anti‐microbial treatments used to control S. Dublin infections in humans in Brazil. 相似文献
58.
PB Fernandes KO Marques FR de Araujo Neto DP de Oliveira NA Hurtado‐Lugo RR Aspilcueta‐Borquis H Tonhati 《Reproduction in domestic animals》2016,51(3):428-434
Because of the importance of reproduction in stock breeding systems, it is necessary to find selection criteria that increase reproductive efficiency. The aim of this study was to estimate genetic parameters for the probability of conception on first service (PROB) in Murrah heifers, and its association with other traits of economic interest [age at first calving (AFC), service period, calving interval and milk yield at 270 days], with the purpose of evaluating their use as selection criteria. Reproductive information and first lactation records of 1200 Murrah heifers were used to perform two‐trait analyses between PROB and the other characteristics. Bayesian inference was used to estimate the variance components, considering PROB as threshold and the other as linear factors. The results demonstrate that this trait has heritability of 0.15, indicating the possibility of a genetic gain by using it for selection. With respect to the genetic correlation estimates, the only high‐magnitude association was with AFC (?0.899), which is the current criterion indicating sexual precocity of females. In the light of the parameters estimated, the first‐service pregnancy rate is an alternative for indication of sexual precocity, although presenting a smaller genetic gain than the current standard AFC. Nevertheless, additional research should be conducted regarding this trait to assess the economic importance of its use in dairy buffalo production systems. 相似文献
59.
PC Sereno AL Beck DB Dutheil B Gado HCE Larsson GH Lyon JD Marcot OWM Rauhut RW Sadleir CA Sidor DD Varricchio GP Wilson JA Wilson 《Science (New York, N.Y.)》1998,282(5392):1298-1302
Fossils discovered in Lower Cretaceous (Aptian) rocks in the Tenere Desert of central Niger provide new information about spinosaurids, a peculiar group of piscivorous theropod dinosaurs. The remains, which represent a new genus and species, reveal the extreme elongation and transverse compression of the spinosaurid snout. The postcranial bones include blade-shaped vertebral spines that form a low sail over the hips. Phylogenetic analysis suggests that the enlarged thumb claw and robust forelimb evolved during the Jurassic, before the elongated snout and other fish-eating adaptations in the skull. The close phylogenetic relationship between the new African spinosaurid and Baryonyx from Europe provides evidence of dispersal across the Tethys seaway during the Early Cretaceous. 相似文献
60.
Soybean molasses as an organic carbon source in the farming of Litopenaeus vannamei (Boone, 1931) in a biofloc system 下载免费PDF全文
Carlos Manoel do Espírito Santo Isabela Claudiana Pinheiro Gabriel Fernandes Alves de Jesus José Luiz Pedreira Mouriño Felipe do Nascimento Vieira Walter Quadros Seiffert 《Aquaculture Research》2017,48(4):1827-1835
Soybean molasses was evaluated as a partial replacement for sugarcane molasses as a carbon source for biofloc development in the superintensive culture of Pacific white shrimp (Litopenaeus vannamei). A 50‐day study was conducted with juvenile (3.2 g) shrimp stocked in 16 800 L tanks at a stocking density of 250 shrimp m?3. Control of total ammonia concentration was performed by the addition of combined mixtures of soybean and sugarcane molasses to the culture water. Three different molasses treatments were evaluated using different soybean‐to‐sugarcane molasses ratios: 15–85%, 38–62% and 60–40% respectively. The control group was treated only with sugarcane molasses. Water quality, chlorophyll a concentration, heterotrophic bacterial load, Vibrio spp. concentration and zootechnical indexes were all evaluated. Total ammonia concentration was controlled by heterotrophic and chemotrophic pathways. Biofloc formation, as quantified by measuring the total suspended solids, was not altered. The Vibrio spp. concentration showed a significant reduction in treatments with soybean‐to‐sugarcane molasses ratios of 38–62% and 60–40%. All combined mixtures of soybean and sugarcane molasses could maintain water quality and productivity in the superintensive culture of L. vannamei using the biofloc system. Thus, the potential use of a residue from agroindustry as a carbon source in a biofloc culture is demonstrated. 相似文献