Myopathy in a Labrador retriever   总被引：3，自引：0，他引：3  

ADJ WATSON  BRH FARROW  DJ MIDDLETON  JBA SMYTH 《Australian veterinary journal》1988,65(7):226-227

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Veterinary Microbiology: Bacterial and Fungal Agents of Animal Disease - By J. Glenn Songer, KW Post     

DJ Trott 《Australian veterinary journal》2006,84(12):438-438

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Vitrification of In Vitro-produced Bovine Embryos by Addition of Ethylene Glycol in One-step     

DJ Walker  LF Campos-Chillon  GE Seidel 《Reproduction in domestic animals》2006,41(5):467-471

The objective of this study was to simplify two-step addition of cryoprotectant for vitrification of bovine embryos by developing a one-step procedure. Survival was calculated as a percentage of non-vitrified controls developed from the same batch of oocytes. In experiment 1, bovine blastocysts were vitrified following one- or two-step addition of cryoprotectant. Exposure of embryos to cryoprotectant in one-step resulted in survival rates not significantly lower (p > 0.1) than those obtained by two-step addition (85% vs 98%, respectively). Based on these results, experiments 2-4 were designed to test one-step addition of cryoprotectant more rigorously. Experiment 2 exposed day 7 blastocysts to 6, 7 or 8 M ethylene glycol for 2.5 or 3.5 min. At 24 h post-vitrification, survival of embryos was similar, irrespective of ethylene glycol concentration or exposure time (6 M 38%, 7 M 51%, 8 M 59%; 2.5 min 54%, 3.5 min 45%). In experiment 3, blastocysts were exposed to 7 M ethylene glycol for shorter times (30 or 60 s); 30 s exposure resulted in decreased survival (8% vs 31%, p < 0.05). Experiment 4 concerned one-step addition of cryoprotectant to day 6 bovine morulae, exposed to 7 M ethylene glycol for 1 or 1.5 min. There was no difference in survival between exposure times of 1 or 1.5 min (28% vs 45%, respectively; p > 0.1). It is unclear why many embryos survive vitrification with one-step addition of cryoprotectant, but others do not. Although, one-step addition of cryoprotectant simplifies the vitrification procedure, survival rates were inadequate for routine cryopreservation of in vitro-produced bovine embryos.  相似文献   

Elasticity of single-crystal MgO to 8 gigapascals and 1600 kelvin     

G Chen  RC Liebermann  DJ Weidner 《Science (New York, N.Y.)》1998,280(5371):1913-1916

The cross pressure (P) and temperature (T) dependence of the elastic moduli (Cij) of single-crystal samples of periclase (MgO) from acoustic wave travel times was measured with ultrasonic interferometry: partial differential2C11/ partial differentialP partial differentialT = (-1.3 +/- 0.4) x 10(-3) per kelvin; partial differential2C110/ partial differentialP partial differentialT = (1. 7 +/- 0.7) x 10(-3) per kelvin; and partial differential2C44/ partial differentialP partial differentialT = (-0.2 +/- 0.3) x 10(-3) per kelvin. The elastic anisotropy of MgO decreases with increasing pressure at ambient temperature, but then increases as temperature is increased at high pressure. An assumption of zero cross pressure and temperature derivatives for the elastic moduli underestimates the elastic anisotropy and overestimates the acoustic velocities of MgO at the extrapolated high-pressure and high-temperature conditions of Earth's mantle.  相似文献   

Pituitary Receptors for GnRH, Oestradiol and Prolactin and the Control of Gonadotrophin Secretion in the Ewe   总被引：3，自引：0，他引：3  

J Brooks  C Sheng  DJ Tortonese  AS McNeilly 《Reproduction in domestic animals》1999,34(3-4):133-138

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Diagnosis and excision of a brachial plexus nerve sheath tumour in a dog     

DJ SIMPSON  JA BECK  GS ALLAN  JA CULVENOR 《Australian veterinary journal》1999,77(4):222-224

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Effects of age on the electroencephalographic response to castration in lambs anaesthetised using halothane in oxygen     

CB Johnson  KJ Stafford  SP Sylvester  RN Ward  S Mitchinson  DJ Mellor 《New Zealand veterinary journal》2013,61(6):433-437

AIM: To use the electroencephalogram (EEG) to ascertain whether the response of the cerebral cortex to the noxious stimulus of castration varied with age in lambs.

METHODS: Two groups of East Friesian lambs were selected according to age; the mean age of the younger group (n=21) was 12 (SD 2) days and the older group (n=20) was 29 (SD 1) days. Anaesthesia was induced via mask using 4% halothane in oxygen, and maintained using 1.5% halothane in oxygen at a flow rate of 4 L/min. Once a stable plane of anaesthesia had been achieved, data collection of EEG and electrocardiographic (ECG) readings commenced, and the lambs were castrated 15 min later, using rubber rings. Median and 95% spectral edge frequencies (F95) and total EEG power (ptot) were derived from data from the EEG.

RESULTS: Following castration, there was an increase in the median frequency (F50) in the younger lambs (p=0.002), and an increase in ptot in both groups (p=0.05), which was of greater magnitude in the older lambs. There were no significant changes in the F95. Both younger and older lambs exhibited a transient bradycardia (p=0.001 and p=0.01, respectively).

CONCLUSIONS: These differences in the cortical response between the two groups suggest that 2-week-old lambs undergo a qualitatively different perception of the noxious stimulus of castration compared to 4-week-old lambs.  相似文献   

The welfare significance of the castration of cattle: A review     

KJ Stafford  DJ Mellor 《New Zealand veterinary journal》2013,61(5):271-278

Castration is an ancient husbandry procedure used to produce docile cattle for draught work, to reduce unwanted breeding, and to modify carcass quality. All the physical methods used to castrate cattle have side-effects and cause pain. The plasma cortisol response to castration using Burdizzo clamps and, by inference, the acute pain experienced, is less than that caused by surgical, rubber-ring or latex-band castration. The cortisol response may be influenced by the age of the animal castrated, but this has not been well defined. Local anaesthesia virtually eliminates the cortisol response, and thus the acute pain, caused by rubber-ring or latex-band castration, but needs to be combined with a systemic analgesic such as the non-steroidal anti-inflammatory drug ketoprofen to eliminate the cortisol response to Burdizzo or surgical castration. When used alone, ketoprofen sometimes reduces the cortisol response to Burdizzo or surgical castration but may need to be accompanied by local anaesthesia to eliminate the pain-induced behaviour seen during the castration process itself. Thus, pharmacological methods are available to virtually eliminate the acute pain experienced by calves during the 12 h following castration. The use of these methods is an additional cost for farmers and may be limited by the availability of drugs for farmers to use and the scarcity of veterinarians in farm animal practice.  相似文献   

A Magnetic Signature at Io: Initial Report from the Galileo Magnetometer   总被引：1，自引：0，他引：1  

MG Kivelson  KK Khurana  RJ Walker  CT Russell  JA Linker  DJ Southwood  C Polanskey 《Science (New York, N.Y.)》1996,273(5273):337-340

During the inbound pass of the Galileo spacecraft, the magnetometer acquired 1 minute averaged measurements of the magnetic field along the trajectory as the spacecraft flew by Io. A field decrease, of nearly 40 percent of the background jovian field at closest approach to Io, was recorded. Plasma sources alone appear incapable of generating perturbations as large as those observed and an induced source for the observed moment implies an amount of free iron in the mantle much greater than expected. On the other hand, an intrinsic magnetic field of amplitude consistent with dynamo action at Io would explain the observations. It seems plausible that Io, like Earth and Mercury, is a magnetized solid planet.  相似文献   

Echinococcus granulosus and other intestinal helminths: current status of prevalence and management in rural dogs of eastern Australia     

DJ Jenkins  JJ Lievaart  B Boufana  WS Lett  H Bradshaw  MT Armua‐Fernandez 《Australian veterinary journal》2014,92(8):292-298

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