Presence of chlorogenic acid during in vitro maturation protects porcine oocytes from the negative effects of heat stress     

Thanh‐Van Nguyen  Lanh Thi Kim Do  Tamas Somfai  Takeshige Otoi  Masayasu Taniguchi  Kazuhiro Kikuchi 《Animal Science Journal》2019,90(12):1530-1536

Chlorogenic acid (CGA) is known to protect oocytes from oxidative stress. Here we investigated the effects of CGA on porcine oocyte maturation under heat stress and subsequent embryonic development after parthenogenetic activation. For in vitro maturation (IVM) at 41.0°C (hyperthermic condition), supplementation of the maturation medium with 50 μM CGA significantly improved the percentage of matured oocytes and reduced the rate of apoptosis relative to oocytes matured without CGA (p < .05). CGA treatment of oocytes during IVM under hyperthermia tended to increase (p < .1) percentage of blastocyst formation after parthenogenesis and significantly increased (p < .05) the total cell number per blastocyst relative to oocytes matured without CGA. For IVM at 38.5°C (isothermic condition), CGA significantly improved the rate of blastocyst development compared with oocytes matured without CGA (p < .05), but did not affect oocyte maturation, apoptosis rate or the number of cells per embryo. Omission of all antioxidants from the IVM medium significantly reduced the rate of oocyte maturation, but the rate was restored upon addition of CGA. These results demonstrate that CGA is a potent antioxidant that protects porcine oocytes from the negative effects of heat stress, thus reducing the frequency of apoptosis and improving the quality of embryos.  相似文献   

Effects of duration of electric pulse on in vitro development of cloned cat embryos with human artificial chromosome vector          下载免费PDF全文

LTK Do  M Wittayarat  T Terazono  Y Sato  M Taniguchi  F Tanihara  T Takemoto  Y Kazuki  K Kazuki  M Oshimura  T Otoi 《Reproduction in domestic animals》2016,51(6):1039-1043

The current applications for cat cloning include production of models for the study of human and animal diseases. This study was conducted to investigate the optimal fusion protocol on in vitro development of transgenic cloned cat embryos by comparing duration of electric pulse. Cat fibroblast cells containing a human artificial chromosome (HAC) vector were used as genetically modified nuclear donor cells. Couplets were fused and activated simultaneously with a single DC pulse of 3.0 kV/cm for either 30 or 60 μs. Low rates of fusion and embryo development to the blastocyst stage were observed in the reconstructed HAC‐transchromosomic embryos, when the duration of fusion was prolonged to 60 μs. In contrast, the prolongation of electric pulse duration improved the embryo development and quality in the reconstructed control embryos without HAC vector. Our results suggested that the optimal parameters of electric pulses for fusion in cat somatic cell nuclear transfer vary among the types used for donor cells.  相似文献   

Epidemiological aspects of lice (Menacanthus species) infections in laying hen flocks from the State of Minas Gerais,Brazil     

L. Do Carmo Rezende  N. R. Da Silva Martins  C. M. Teixeira  P. R. De Oliveira 《British poultry science》2016,57(1):44-50

1. The epidemiology of chicken lice species such as Menacanthus stramineus, M. cornutus and M. pallidulus were studied during an observational, analytical and sectional survey, to determine predisposing factors for their occurrence in laying hen farms in the State of Minas Gerais, Brazil. A total of 431 houses on 43 farms were visited in 2012.

2. M. cornutus, M. stramineus and M. pallidulus occurred in 20.9%, 11.6% and 11.6% of farms, respectively. The frequencies of occurrence of M. cornutus, M. stramineus and M.pallidulus in poultry houses were 10.4%, 8.8% and 3.7%, respectively.

3. The epidemiological determinants for the occurrence of these species were investigated using Poisson or logistic regression models.

4. The region of the farm, the recent use of acaricides and the presence of birds, such as saffron finch (Sicalis flaveola), feral pigeon (Columba livia) and Guira cuckoo (Guira guira) around the farms were related to the epidemiology of M. cornutus.

5. Infestation by M. stramineus was associated with age of birds, number of birds per cage and the presence of Guira cuckoo and Chopi blackbird (Gnorimopsar chopi) near the poultry houses.

6. The occurrence of M. pallidulus was influenced by the type of facilities, presence of cattle egret (Bubulcus ibis) and free-range domestic hens around the farm.

7. The use of wire mesh nets in the houses and of forced moulting did not influence lice infestation.

  相似文献   

Genetic parameters for carcass traits and body size in sheep for meat production     

Luiz Antonio Silva Figueiredo Filho  Alan Oliveira Do Ó  José Lindenberg Rocha Sarmento  Natanael Pereira Da Silva Santos  Tatiana Saraiva Torres 《Tropical animal health and production》2016,48(1):215-218

  相似文献   

Pharmacokinetics and pharmacodynamics of intravenous esomeprazole at 2 different dosages in dogs     

Do‐Hyun Seo  Jong‐Bok Lee  Ji‐Hye Hwang  Jong‐Woo Jeong  Gun‐Ho Song  Tae‐Sung Koo  Kyoung‐Won Seo 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(2):531-535

  相似文献   

Interactions between fawns and does of farmed fallow deer Dama dama in the postnatal period          下载免费PDF全文

Paweł Janiszewski  Justyna Cilulko‐Dołęga  Daria Murawska  Marek Bogdaszewski 《Animal Science Journal》2018,89(2):483-487

The aim of the present study was to describe the suckling behavior of the offspring of farmed European fallow deer Dama dama in the postnatal period. Video surveillance footage from the first 18 days postpartum was analyzed in detail to reveal that the duration of interactions between the mother and the offspring changed with the fawns’ age. The duration of mother‐offspring interactions was longest between postpartum days 12 and 18. The frequency of interactions at different times of the day varied in the analyzed periods. The results of the study could be used to increase the welfare of farmed deer.  相似文献   

Digested soybean protein and taurine influence bile acid level,lipase activity,lipid digestibility,and growth performance of pompano (Trachinotus blochii)     

Nguyen  Hung Phuc  Van Do  Thinh 《Fish physiology and biochemistry》2021,47(4):1199-1209

Fish Physiology and Biochemistry - This study aimed to examine the effects of dietary digested soybean protein (DSP) and taurine on bile acid (BA) level, lipase activity, lipid apparent...  相似文献   

Expression of neurotrophic factors in injured spinal cord after transplantation of human-umbilical cord blood stem cells in rats     

Hyo-jin Chung  Wook-hun Chung  Jae-Hoon Lee  Dai-Jung Chung  Wo-Jong Yang  A-Jin Lee  Chi-Bong Choi  Hwa-Seok Chang  Dae-Hyun Kim  Hyun Jung Suh  Dong-Hun Lee  Soo-Han Hwang  Sun Hee Do  Hwi-Yool Kim 《Journal of veterinary science (Suw?n-si, Korea)》2016,17(1):97-102

We induced percutaneous spinal cord injuries (SCI) using a balloon catheter in 45 rats and transplanted human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) at the injury site. Locomotor function was significantly improved in hUCB-MSCs transplanted groups. Quantitative ELISA of extract from entire injured spinal cord showed increased expression of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF) and neurotrophin-3 (NT-3). Our results show that treatment of SCI with hUCB-MSCs can improve locomotor functions, and suggest that increased levels of BDNF, NGF and NT-3 in the injured spinal cord were the main therapeutic effect.  相似文献   

Isolation and antifungal activity of lignans from Myristica fragrans against various plant pathogenic fungi     

Cho JY  Choi GJ  Son SW  Jang KS  Lim HK  Lee SO  Sung ND  Cho KY  Kim JC 《Pest management science》2007,63(9):935-940

BACKGROUND: In a search for plant extracts with potent in vivo antifungal activity against various plant diseases, we found that treatment with a methanol extract of Myristica fragrans Houttyn (nutmeg) seeds reduced the development of various plant diseases. The objectives of the present study were to isolate and determine antifungal substances from My. fragrans and to evaluate their antifungal activities. RESULTS: Three antifungal lignans were isolated from the methanol extract of My. fragrans seeds and identified as erythro-austrobailignan-6 (EA6), meso-dihydroguaiaretic acid (MDA) and nectandrin-B (NB). In vitro antimicrobial activity of the three lignans varied according to compound and target species. Alternaria alternata, Colletotrichum coccodes, C. gloeosporioides, Magnaporthe grisea, Agrobacterium tumefaciens, Acidovorax konjaci and Burkholderia glumae were relatively sensitive to the three lignans. In vivo, all three compounds effectively suppressed the development of rice blast and wheat leaf rust. In addition, EA6 and NB were highly active against the development of barley powdery mildew and tomato late blight, respectively. Both MDA and NB also moderately inhibited the development of rice sheath blight. CONCLUSION: This is the first study to demonstrate the in vitro and in vivo antifungal activities of the three lignans from My. fragrans against plant pathogenic fungi.  相似文献   

PI(3,4,5)P3 and PI(4,5)P2 lipids target proteins with polybasic clusters to the plasma membrane     

Heo WD  Inoue T  Park WS  Kim ML  Park BO  Wandless TJ  Meyer T 《Science (New York, N.Y.)》2006,314(5804):1458-1461

Many signaling, cytoskeletal, and transport proteins have to be localized to the plasma membrane (PM) in order to carry out their function. We surveyed PM-targeting mechanisms by imaging the subcellular localization of 125 fluorescent protein-conjugated Ras, Rab, Arf, and Rho proteins. Out of 48 proteins that were PM-localized, 37 contained clusters of positively charged amino acids. To test whether these polybasic clusters bind negatively charged phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] lipids, we developed a chemical phosphatase activation method to deplete PM PI(4,5)P2. Unexpectedly, proteins with polybasic clusters dissociated from the PM only when both PI(4,5)P2 and phosphatidylinositol 3,4,5-trisphosphate [PI(3,4,5)P3] were depleted, arguing that both lipid second messengers jointly regulate PM targeting.  相似文献