Differential effects of follistatin on porcine oocyte competence and cumulus cell gene expression in vitro          下载免费PDF全文

Z Guo  D Liu  G Liu  L Lv  Y Yang  B Fu  L Wang  Z Liu  H He  H Wu 《Reproduction in domestic animals》2018,53(1):3-10

  相似文献   

Bovine foetal sex determination—Different DNA extraction and amplification approaches for efficient livestock production          下载免费PDF全文

M Ristanic  Lj Stanisic  M Maletic  U Glavinic  V Draskovic  N Aleksic  Z Stanimirovic 《Reproduction in domestic animals》2018,53(4):947-954

Foetal sex determination using polymerase chain reaction (PCR) in mammals is based on the amplification of gender‐specific foetal DNA sequences circulating in maternal blood. The bovine synepitheliochorial placenta does not allow a direct contact between the trophoblast and the maternal blood, resulting in difficult passage of foetal DNA and, consequently, its very small amounts in maternal bloodstream. Circulating cell‐free foetal DNA (ccffDNA) encompasses short nucleotide fragments (300–600 bp) in maternal circulation. The aim of this study was to assess this non‐invasive method in accurate prenatal sexing in early and late gestational periods in comparison with ultrasound diagnostics. As various DNA isolation and amplification methods were tested, their success in obtaining reliable results was evaluated. Two groups were tested, each consisting of 20 pregnant cows. Blood of a bull and a non‐pregnant heifer was the controls. Extraction of foetal DNA was accomplished by three different methods: using tubes with silicone membranes, a single‐tube extraction without silicone membranes and phenol–chloroform extraction. Following each extraction method, foetal DNA was amplified using PCR and real‐time PCR with both bAML and TSPY primers in a separate reaction. Positive results were obtained only after amplification of foetal DNA extracted with a single‐tube extraction kit. In comparison with ultrasound examination results and foetal gender recorded at birth, the sensitivity of the PCR test was 90% in Group I, but the technique failed to detect male foetuses in Group II. The real‐time PCR test sensitivity in Group I was 90% and in Group II 91.6%.  相似文献   

Comparative expression profile of microRNAs and piRNAs in three ruminant species testes using next‐generation sequencing          下载免费PDF全文

H Wang  J Zhong  Z Chai  J Zhu  J Xin 《Reproduction in domestic animals》2018,53(4):963-970

microRNA (miRNA) and piwi‐interacting RNA (piRNA) are two classes small non‐coding regulatory RNAs that play crucial roles in multiple biological processes such as spermatogenesis. However, there are no published studies on conjoint analysis of miRNA and piRNA profiles among cattle, yak and their interspecies (the dzo) using sequencing technology. Next‐generation sequencing technology was used to profile miRNAs and piRNAs among those three ruminants to elucidate their functions. A total of 119, 14 and six differentially expressed miRNAs were obtained in cattle vs. dzo, cattle vs. yak and yak vs. dzo comparison groups, while there were 873, 1,065 and 1,158 differentially expressed piRNAs in those three comparison groups. The expression of three miRNAs was validated in the three ruminants, and the results suggested that the miRNA expression profiles data could represent actual miRNA expression levels. Moreover, the putative targets of differentially expressed miRNAs were predicted by their own genome, it is worth to note that both the cattle and yak genome were used for dzo, then the targets were subjected to GO enrichment and KEGG pathway analysis, revealing the likely roles for these differentially expressed miRNAs in spermatogenesis. In conclusion, this study provided a useful resource for further elucidation of the miRNAs and piRNAs regulatory roles in spermatogenesis. It may also facilitate the development of therapeutic strategies for dzo reproduction research.  相似文献   

Caecal intussusceptions and typhlocolitis in horses with severe Gastrodiscus aegyptiacus infestation          下载免费PDF全文

Z. Gratwick  C. Donnellan  P. C. Page  A. Viljoen  J. Williams  C. H. Lyle 《Equine Veterinary Education》2018,30(5):247-254

The intestinal trematode Gastrodiscus aegyptiacus (G. aegyptiacus) has been recognised in equids around the world for many years, but its pathogenicity is yet to be confirmed. This report describes seven cases of severe G. aegyptiacus infestation, including six cases of caecal intussusception.  相似文献   

Genetic Analysis and Gene Mapping of a Yellow-green Leaf Mutant w390 in Rice (Oryza sativa L.)     

Qing DONG  Ying-xin ZHANG  Zhen-hua ZHANG  Quan ZHOU  Ya-zhi QIN  Hong WANG  Shi-hua CHENG  Li-yong CAO  Xi-hong SHEN 《中国水稻科学》2015,29(3):241

  相似文献   

穴盘规格对5种苔草属植物育苗的影响     

梁芳  董爱香  李子敬  辛海波  赵正楠 《草业科学》2015,32(10):1625-1630

穴盘育苗是苔草繁育的重要技术环节。为加快苔草的繁育速度,采用不同规格穴盘播种以研究青绿苔草(Carex breviculmis)、涝峪苔草(C.giraldiana)、披针苔草(C.lanceolata)、矮丛苔草(C.humilis var.nana)、脚苔草(C.pediformis)的适宜播种穴盘孔数对5种苔草出苗和幼苗生长的影响。通过对各种苔草的开始发芽时间、持续发芽时间、发芽率和幼苗的株高、叶数、单株鲜重等生长指标的分析,结果表明,6月温室穴盘播种育苗,青绿苔草、涝峪苔草穴盘育苗128孔或105孔穴盘较为适宜;披针苔草穴盘育苗宜采用105孔穴盘;矮丛苔草、脚苔草穴盘育苗较适宜选择288孔穴盘。  相似文献   

7株根瘤菌菌株与热研2号柱花草共生最适磷浓度研究     

董荣书  张洁  黄艳霞  白昌军  郇恒福  王文强  刘国道 《热带作物学报》2014,35(5):832-836

采用水培的方法,设3个磷水平,7个菌株,通过对株高、茎叶鲜重、茎叶干重、固氮酶活性、茎叶氮含量、茎叶磷含量的测定及综合分析,确定使热研2号柱花草接种不同根瘤菌菌株达到最佳促生性能的磷浓度。结果表明:7个菌株在中磷时鲜重和含氮量最大,低磷不利于鲜重和含氮量的增加;经主因子综合分析,菌株YM11-1、RJS9-2、PN13-3在高磷时对热研2号柱花草有最好的固氮促生效果,菌株LZ3-2、BS1-1、PN13-3在中等磷条件下有最好的固氮促生效果,菌株FS3-1-1在高磷和中磷都有最好的固氮促生效果。  相似文献   

农业科研单位年鉴编纂工作的思考     

张春锋  闫芳教  董立民  阎立波  郭伟  张建军 《河北农业科学》2011,15(3):166-167

结合河北省农林科学院年鉴编纂工作的实际情况,针对存在的部分问题,提出健全编辑队伍、加强业务培训、完善激励机制、重视编校质量、创新编纂工作的建议。  相似文献   

Copper‐Induced Oxidative Stress and Responses of the Antioxidant System in Roots of Medicago sativa     

S.‐H. Wang  H. Zhang  Q. Zhang  G.‐M. Jin  S.‐J. Jiang  D. Jiang  Q.‐Y. He  Z.‐P. Li 《Journal of Agronomy and Crop Science》2011,197(6):418-429

The effects of various copper (Cu) concentrations on the antioxidative system in the roots of Medicago sativa were explored. The results indicated that the Cu content of the roots reached a value of 854 μg g^?1 DW at 10 μm Cu and a value of 4415 μg g^?1 DW at 100 μm Cu, suggesting that M. sativa has better ability to tolerate and accumulate Cu than other Cu‐bioaccumulators, and is a potential plant for phytoremediation. Treatment with Cu resulted in a significant increment in the levels of H₂O₂, O₂˙^? and OH˙. The reduced form of ascorbate and glutathione reached a peak at 30 μm Cu, and was followed by a sharp depletion to a lower level than that of the control. In contrast, the levels of the oxidised forms of ascorbate and glutathione showed a progressive increment with increasing Cu concentrations, suggesting that the antioxidant system was unable to cope with Cu stress at higher Cu levels. Under the Cu concentrations tested, the activity of catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC 1.6.4.2) increased at lower Cu concentrations, and then decreased, reaching a maximum at 30 μm of Cu for APX and GR, at 10 μm for CAT, whereas the activities of guaiacol peroxidase (POD, EC 1.11.1.7) were gradually increased with increasing Cu concentrations. PAGE analysis of superoxide dismutase (SOD, EC 1.1.5.1.1) revealed that one band is a Mn‐SOD and five bands are identified as Cu, Zn‐SOD, whereas Fe‐SOD isoforms were not found in the roots of alfalfa. Cu at 10–100 μm increased the intensity of constitutive isozymes of CAT, APX and POD, whereas it decreased the intensity of isozymes of glucose‐6‐phosphate dehydrogenase (G6PDH, EC 1.1.1.49) significantly. The activities of lipoxygenases (LOX, EC 1.13.11.12) were gradually augmented with increasing Cu concentrations, demonstrating that LOXs are probably involved in production of lipid hydroperoxides and superoxide anion. There was a continuous and pronounced enhancement in the activity of esterase (EST, EC 3.1.1.1) in roots treated with 10–30 Cu μm , whereas EST activity in roots exposed to above 30 μm Cu declined, suggesting that EST plays a protective role under lower Cu concentrations stress.  相似文献   

Identification and Expression Analysis of MYB Transcription Factors Related to Rust Resistance in Foxtail Millet   总被引：1，自引：1，他引：0  

BAI Hui  SONG ZhenJun  WANG YongFang  QUAN JianZhang  MA JiFang  LIU Lei  LI ZhiYong  DONG ZhiPing 《中国农业科学》2019,52(22):4016-4026

  相似文献