An estimate of specificity for a Johne's disease absorbed ELISA in northern Australian cattle     

DJ PITT  DS PINCH  A. JANMAAT  RJ CONDRON 《Australian veterinary journal》2002,80(1):57-60

Objective To estimate the specificity of an absorbed enzyme-linked immuno-sorbent assay kit^d for Johne's disease (JD) when used in mature cattle populations resident in northern Australia.
Design Blood samples were collected from beef cattle in northern Queensland, the Northern Territory and northern Western Australia, and from dairy cattle in northern Queensland. The specificity of a serological test for JD was estimated by testing the blood samples with an absorbed ELISA kit. Further samples were collected from cattle with positive ELISA results to determine the presence or absence of infection with Mycobacterium avium subsp paratuberculosis .
Procedure During 1995 and 1996, blood, tissue and gut contents were collected from beef cattle at abattoirs in Queensland and the Northern Territory; and blood and faecal samples were collected from dairy cattle in herds assessed to be most at risk for JD in northern Queensland. The blood samples were tested using an absorbed ELISA kit. Tissues and gut contents from beef cattle that had positive ELISA results were cultured for M avium subsp paratuberculosis , and tissues were examined histo-logically. Faecal samples from dairy cattle with positive ELISA results were cultured for M avium subsp paratuberculosis .
Results Estimates of specificity for this absorbed ELISA in mature northern Australian cattle were 98.0% (97.0 to 98.8%, 95% CI) in beef cattle, and 98.3% (96.7 to 99.3%, 95% CI) in dairy cattle.
Conclusion Estimates of specificity in this study were lower for beef cattle from the Northern Territory and northern Western Australia and for dairy cattle from northern Queensland than those quoted from studies on cattle in southern Western Australia. This should be considered when serological testing using the JD ELISA is carried out on northern Australian cattle.  相似文献   

Cadmium concentrations in the kidneys of Western Australian pigs     

PW MORCOMBE  P ROSS  HG MASTERS  DS PETTERSON  A ISOPENKO 《Australian veterinary journal》1992,69(5):113-114

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Treatment with vitamins A, D and E did not reduce weight loss in transported cattle     

TF JUBB  DS PINCH†  SR PETTY 《Australian veterinary journal》1993,70(5):171-173

SUMMARY Two field trials using an injectable vitamin A, D and E solution conducted in the pastoral environment of northern Australia are described. It was found that treatment of lighter (< 150 kg) or heavier (> 372 kg) weight cattle did not reduce weight loss during road transport. These findings contradict anecdotal evidence of reduced weight loss during transport with the use of vitamins A, D and E. The lack of scientific basis for their use is discussed.  相似文献   

The Regulation of Aromatase and Androgen Receptor Expression During Gonad Development in Male and Female European Eel     

DS Peñaranda  I Mazzeo  V Gallego  J Hildahl  R Nourizadeh‐Lillabadi  L Pérez  F‐A Weltzien  JF Asturiano 《Reproduction in domestic animals》2014,49(3):512-521

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Analgesic effect of pre-operative etodolac and butorphanol administration in dogs undergoing ovariohysterectomy     

T  Inoue  JCH Ko  RE Mandsager  ME Payton  DS Galloway  DN Lange 《Veterinary anaesthesia and analgesia》2003,30(2):109-110

The analgesic, bleeding, and renal effects of dogs pre‐medicated with etodolac with and without butorphanol were evaluated. Twenty‐four 1‐year‐old healthy dogs, weighing 19 ± 3 kg (mean ± SD) were randomly assigned to four treatment groups (n = 6): control (C), etodolac (E), butorphanol (B), and etodolac with butorphanol (EB). Etodolac (12–14 mg kg^?1 PO) was given 1 hour before propofol induction and isoflurane maintenance anesthesia. Butorphanol (0.4 mg kg^?1 IV) was given immediately following endotracheal intubation. Control dogs received only propofol (8 mg kg^?1 to effect) and isoflurane anesthesia. All dogs were mechanically ventilated to maintain Pe ′CO₂ between 35 and 45 mm Hg (4.7–6.0 kPa). Lactated Ringer's solution was given at 10 mL kg^?1 hour^?1 during anesthesia. Plasma cortisol concentrations were assessed 1 day prior to surgery (baseline), immediately prior to anesthesia induction, and every 30 minutes until 5 hours following extubation, and 1 day after surgery. Total duration of anesthesia was 50 minutes and total surgery duration was 30 minutes. Isoflurane concentration area under the curve (AUC) over time during the anesthesia was compared among treatment groups. Buccal mucosal bleeding time (BMBT) was assessed 1 day before E administration and during surgery. Urine GGT to urine creatinine ratio, BUN, and plasma creatinine were taken daily from 1 day before to 3 days after surgery. Behavioral pain scores (numerical rating scale) were assessed by two observers blinded to the treatment during the 5‐hour recovery period at 30 minute intervals until 3 hours, and again at 5 hours after extubation. All data were analyzed using anova . Multiple comparisons were performed if the anova was significant. Alpha value was set at 0.05. Plasma cortisol concentrations significantly increased from time of extubation in all the treatment groups. They did not return to the baseline until 5, 2.5, 1.5, and 1.5 hours after extubation in the C, B, E, and EB groups, respectively. Isoflurane AUC was not significantly different among treatment groups. Dogs treated with EB had significantly less behavioral pain than all other groups throughout the 5‐hour recovery period. No significant difference was found between treatment groups or within treatment groups over time in BMBT, or any renal variables. This study demonstrated that (i) pre‐operative administration of E provides profound analgesia during the post‐operative period without renal or bleeding side‐effects in dogs undergoing OHE; and (ii) a combination of butorphanol–etodolac provides the best analgesic effect during the post‐operative period based on the behavioral pain score.  相似文献   

Effect of a second treatment of prostaglandin F2α during the Ovsynch program on fixed-time artificial insemination conception rates and luteolysis in split-calving,pasture-fed dairy cows     

JM Rheinberger  DD Colson  DS Beggs  PD Mansell  MA Stevenson  RJ Rheinberger  MF Pyman 《Australian veterinary journal》2020,98(5):190-196

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Variant of SCN5A sodium channel implicated in risk of cardiac arrhythmia     

Splawski I  Timothy KW  Tateyama M  Clancy CE  Malhotra A  Beggs AH  Cappuccio FP  Sagnella GA  Kass RS  Keating MT 《Science (New York, N.Y.)》2002,297(5585):1333-1336

Every year, approximately 450,000 individuals in the United States die suddenly of cardiac arrhythmia. We identified a variant of the cardiac sodium channel gene SCN5A that is associated with arrhythmia in African Americans (P = 0.000028) and linked with arrhythmia risk in an African-American family (P = 0.005). In transfected cells, the variant allele (Y1102) accelerated channel activation, increasing the likelihood of abnormal cardiac repolarization and arrhythmia. About 13.2% of African Americans carry the Y1102 allele. Because Y1102 has a subtle effect on risk, most carriers will never have an arrhythmia. However, Y1102 may be a useful molecular marker for the prediction of arrhythmia susceptibility in the context of additional acquired risk factors such as the use of certain medications.  相似文献   

Dynamical Signature of the Mott-Hubbard Transition in Ni(S,Se)2     

A Husmann  DS Jin  YV Zastavker  TF Rosenbaum  X Yao  JM Honig 《Science (New York, N.Y.)》1996,274(5294):1874-1876

The transition metal chalcogenide Ni(S,Se)2 is one of the few highly correlated, Mott-Hubbard systems without a strong first-order structural distortion that normally cuts off the critical behavior at the metal-insulator transition. The zero-temperature (T) transition was tuned with pressure, and significant deviations were found near the quantum critical point from the usual T1/2 behavior of the conductivity characteristic of electron-electron interactions in the presence of disorder. The transport data for pressure and temperature below 1 kelvin could be collapsed onto a universal scaling curve.  相似文献   

Tri‐partite complexity: odour from a psyllid's mutualist ant increased predation by a predatory mite on the psyllid     

Jian‐Feng Liu  Zhi‐Qiang Zhang  Jacqueline R Beggs 《Pest management science》2019,75(5):1317-1327

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Factors associated with colostrum immunoglobulin G concentration in northern‐Victorian dairy cows          下载免费PDF全文

AJ Phipps  DS Beggs  AJ Murray  PD Mansell  MF Pyman 《Australian veterinary journal》2017,95(7):237-243

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