Porcine Conceptus Oct-4 mRNA Expression During Peri-implantation Development     

DS Spencer  JW Ross  MD Ashworth  RD Geisert  LF Rickords 《Reproduction in domestic animals》2006,41(6):571-572

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Heavy metals in horse blood, serum, and feed in Minas Gerais, Brazil     

Leandro Maia  Maria Vernica de Souza MV  MS  DS  Raphael Bragana Alves Fernandes BS  MS  DS  Maurício Paulo Ferreira Fontes BS  MS  PhD  Matheus Werner de Souza Vianna  Walcrisley Vercelli Luz BS  MS 《Journal of Equine Veterinary Science》2006,26(12):578-583

Pollution is a subject of worldwide concern and discussion and various areas of science are seeking to integrate their knowledge in an attempt to improve understanding of the harmful effects of modern human activities. Thus, the objective of the current study was to assess the potential for using the horse as a bio-indicator of environmental pollution. Blood and serum samples from 120 horses from locations with different degrees of urbanization and industrialization and the feeds used for these animals were analyzed to determine their heavy metal (Cd, Pb, Ni, Cu, and Zn) content. The data obtained were compared. The results did not show increases in heavy metal concentration in the blood and serum of the horses with the intensification of urbanization and industrialization around the farms. Only Cd, as determined in the blood of the animals, showed a certain potential for using horses as bio-indicators of environmental pollution. The data for sex, age, and chemical composition of the foodstuffs supplied to the animals were not determinants in explaining the variation in the concentrations of heavy metals observed.  相似文献   

Effects of pH, sodium bicarbonate, cryoprotectants and foetal bovine serum on the cryopreservation of European eel sperm.     

DL Garzón  DS Peñaranda  L Pérez  F Marco‐Jiménez  X Espert  T Müller  M Jover  JF Asturiano 《Reproduction in domestic animals》2008,43(1):99-105

The main objective of the present study was to evaluate the influence of pH and bicarbonate concentration in the activation or inhibition of European eel (Anguilla anguilla) spermatozoa and to evaluate the effect of different cryoprotectants: dimethyl sulphoxide (DMSO), acetamide, ethylene glycol, propanol, glycerol and methanol (MeOH). The effect of these factors was evaluated comparing the percentage of motile cells, the percentage of alive cells (by Hoechst staining) and the spermatozoa morphometry pre- and post-cryopreservation (by computer-assisted morphology analysis). Based on the above findings, three cryoprotectants (DMSO, MeOH and glycerol) were chosen and evaluated in two media (P1 and P1 modified) with different concentrations of NaHCO(3) and in the presence or absence of foetal bovine serum (FBS). The effect of these factors was evaluated comparing the percentage of alive and motile cells post-cryopreservation. DMSO was the cryoprotectant showing better results in relation to the percentage of spermatic alive cells post-freezing and caused a smaller modification of the head spermatozoa morphology. The combination of P1-modified medium with DMSO and containing FBS increased slightly but significantly the percentage of motile spermatozoa post-cryopreservation.  相似文献   

Clinical and epidemiological features of West Nile virus equine encephalitis in New South Wales,Australia, 2011     

AJ Read  DS Finlaison  X Gu  PM Hick  BJ Moloney  T Wright  PD Kirkland 《Australian veterinary journal》2019,97(5):133-143

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Factors associated with fertility of nulliparous dairy heifers following a 10‐day fixed‐time artificial insemination program with sex‐sorted and conventional semen          下载免费PDF全文

EJ Noonan  JC Kelly  DS Beggs 《Australian veterinary journal》2016,94(5):145-148

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Low-temperature synthesis of zintl compounds with a single-source molecular precursor     

MA Beswick  N Choi  CN Harmer  AD Hopkins  M McPartlin  DS Wright 《Science (New York, N.Y.)》1998,281(5382):1500-1501

Thermolysis of the heterobimetallic phosphinidene complex [Sb(PCy)3]2- Li6.6HNMe2 (Cy = C6H11) at 303 to 313 kelvin gives Zintl compounds containing (Sb7)3- anions. The complex thus constitutes a stable molecular single-source precursor to Zintl compounds and provides a potential low-temperature route to photoactive alkali metal antimonates. The new chemical reaction involved, which is driven thermodynamically by the formation of P-P bonds, has implications in the low-temperature synthesis of other technologically important materials (such as gallium arsenide).  相似文献   

Homogeneous Linewidths in the Optical Spectrum of a Single Gallium Arsenide Quantum Dot   总被引：1，自引：0，他引：1  

D Gammon  ES Snow  BV Shanabrook  DS Katzer  D Park 《Science (New York, N.Y.)》1996,273(5271):87-90

The homogeneous linewidths in the photoluminescence excitation spectrum of a single, naturally formed gallium arsenide (GaAs) quantum dot have been measured with high spatial and spectral resolution. The energies and linewidths of the homogeneous spectrum provide a new perspective on the dephasing dynamics of the exciton in a quantum-confined, solid-state system. The origins of the linewidths are discussed in terms of the dynamics of the exciton in zero dimensions, in particular, in terms of lifetime broadening through the emission or absorption of phonons and photons.  相似文献   

Early Development and Putative Primordial Germ Cells Characterization in Dogs     

DS Martins  CE Ambrósio  NZ Saraiva  CV Wenceslau  AC Morini  I Kerkis  JM Garcia  MA Miglino 《Reproduction in domestic animals》2011,46(1):e62-e66

Previously, three distinct populations of putative primordial germ cells (PGCs), namely gonocytes, intermediate cells and pre‐spermatogonia, have been described in the human foetal testis. According to our knowledge, these PGCs have not been studied in any other species. The aim of our study was to identify similar PGC populations in canine embryos. First, we develop a protocol for canine embryo isolation. Following our protocol, 15 canine embryos at 21–25 days of pregnancy were isolated by ovaryhysterectomy surgery. Our data indicate that dramatic changes occur in canine embryo development and PGCs specification between 21 to 25 days of gestation. At that moment, only two PGC populations with distinct morphology can be identified by histological analyses. Cell population 1 presented round nuclei with prominent nucleolus and a high nuclear to cytoplasm ratio, showing gonocyte morphology. Cell population 2 was often localized at the periphery of the testicular cords and presented typical features of PGC. Both germ cell populations were positively immunostained with anti‐human OCT‐4 antibody. However, at day 25, all cells of population 1 reacted positively with OCT‐4, whereas in population 2, fewer cells were positive for this marker. These two PGCs populations present morphological features similar to gonocytes and intermediate cells from human foetal testis. It is expected that a population of pre‐spermatogonia would be observed at later stages of canine foetus development. We also showed that anti‐human OCT‐4 antibody can be useful to identify canine PGC in vivo.  相似文献   

Application of a real‐time polymerase chain reaction assay to the diagnosis of bovine ephemeral fever during an outbreak in New South Wales and northern Victoria in 2009–10     

DS Finlaison  AJ Read  J Zhang  R Paskin  PD Kirkland 《Australian veterinary journal》2014,92(1-2):24-27

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A cross-sectional pilot study to estimate the prevalence of and risk factors for leptospirosis in South-Western Victorian dairy herds, 2017     

E Erregger  MA Stevenson  DS Beggs  S Oswin  SP Jagoe  PD Mansell  MF Pyman 《Australian veterinary journal》2020,98(9):417-423

Leptospirosis is a zoonosis, found worldwide, affecting many species of animals. We conducted a cross-sectional study to estimate the prevalence of Leptospira borgpetersenii sv Hardjo and Leptospira interrogans sv Pomona in cattle in dairy herds in South-Western Victoria, Australia. Fifty-three herds were enrolled in the study. Urine samples were collected from 15 late-lactation cows in each herd. A questionnaire was provided to herd managers at the time of each herd visit, asking them to describe the methods they used for controlling leptospirosis, including vaccination. Urine samples were pooled at the herd level and tested for leptospira spp. using real time PCR. Urine samples from individual cows within the positive pooled samples were then tested for Leptospira Hardjo and Leptospira Pomona using qPCR. Four of the 53 herds showed positive leptospirosis results giving an apparent prevalence of 8 (95% CI 2–18) leptospira-positive herds per 100 herds at risk. Based on the 53 completed questionnaires, leptospirosis vaccination programs were not compliant with label directions in 36 of the 52 vaccinated herds: 69 (95% CI 55–81) of 100 herd managers that routinely vaccinated for leptospirosis did not comply with label directions. One herd was completely unvaccinated. Based on our findings, we estimate that approximately 10% of dairy farms in South-Western Victoria are likely to be infected with leptospirosis. While most herds are vaccinating for leptospirosis, most are not doing so according to label directions. We conclude that herd managers need to be better educated regarding leptospirosis vaccination programs.  相似文献