一种适宜棉花microRNA实时定量分析的总RNA提取方法     

余道乾  WANG Gaskin  杜雄明 《棉花学报》2013,25(5):467-470

为满足棉花组织micro RNA结构与功能研究的需要,建立了棉花高质量的总RNA提取方法.以陆地棉纤维和胚珠为供试材料,采用改良的异硫氰酸胍-硫氰酸铵-酸酚法提取总RNA,利用stem-loop RT-qPCR法鉴定成熟的miRNA.结果表明此方法能得到高质量的总RNA,并且miRNA的回收率较高,足以满足mi-croRNA水平的实时定量分析.  相似文献   

杨树生物钟节律基因PtCCA1的克隆及表达模式研究          下载免费PDF全文

刘无双  杜明会  陶维康  杨贞  诸葛强 《林业科学研究》2013,26(5):649-654

生物钟节律基因CCA1在植物的光周期反应中起着重要的调控作用.利用RT-PCR方法克隆获得了一条包含1902 bp开放阅读框的杨树CCA1基因cDNA序列,其编码633个氨基酸残基,蛋白的分子量约为68.90 kDa,等电点为6.33.分析显示含有1个Myb-like DNA结合域及2个蛋白跨膜结合域.实时荧光定量PCR分析发现,PtCCA1基因主要在杨树叶组织中表达;随着光照时间的变化,该基因在杨树中的表达量呈现白昼不断降低而夜晚逐渐升高的昼夜变化趋势.研究结果为进一步探索PtCCA1基因在调控杨树光周期响应途径中的功能以及杨树光周期敏感机制提供了科学基础.  相似文献   

2012年我国蒜薹产业产贮销情况回顾与2013年前景分析          下载免费PDF全文

马岩松  宗艳  宋茂树  杜孟学 《保鲜与加工》2013,13(2):43-48

回顾2012年我国蒜薹产业的产贮销情况,总结蒜薹营销及贮藏的经验教训,剖析2013年蒜薹收贮及销售市场的影响因素,提出今后蒜薹产业可持续发展的一些思考,旨在为我国蒜薹产业的良性发展提供有益的参考与启示。  相似文献   

基于SSR分子标记的杜仲遗传多样性体系建立   总被引：5，自引：3，他引：2       下载免费PDF全文

黄海燕  杜红岩  乌云塔娜  朱高浦 《林业科学研究》2013,26(6):795-799

In order to set up the genetic diversity system of Eucommia ulmoides Oliver based on SSR molecular markers, the establishment of SSR-PCR reaction system and screening out SSR marker primer showing high polymorphism were studied. A L₉(3⁴) orthogonal design was performed to optimize the main factors of the SSR-PCR reaction system. The results indicated that the best SSR-PCR reaction system for E.ulmoides was DNA template 1 μL (30~60 ng·μL^-1), 2×Taq PCR Master Mix 10 μL, primer 1 μL with the total volume of 25 μL. The PCR reaction system had high stability and repeatability, the pairs of SSR primers with high polymorphism were gotten. The 8 E.ulmoides samples' DNA sequence was amplified with 13 pairs of SSR primers by SSR-PCR technique, 34 alleles were detected, 2.6 alleles were detected from per site on average. Each allele's effective number was 1.751 5, and the h value was 0.379 8, the average I value was 0.643 3. This study is helpful in using SSR molecular marker to analyze genetic diversity and genetic relationship in E. ulmoides.  相似文献   

陕西天华山国家自然保护区两栖爬行动物区系特性和分布     

贺亚东  杜选长 《陕西林业科技》2013,(5):13-15,38

通过调查和查阅资料,分析研究了天华山自然保护区两栖爬行动物区系特性和分布,表明天华山自然保护区有两栖动物8种,隶属2目5科6属;爬行动物21种,隶属2目6科17属;区系为以东洋界区系为主,具有复杂性和多样性;两栖爬行动物的垂直分布不明显.对两栖爬行动物的保护提出了建议,以期使两栖爬行动物得到有效保护并充分发挥它们的生态、经济效益.  相似文献   

仿生学及生物力学研究综述     

杜鹏东 《林业机械与木工设备》2013,(9)

介绍了仿生学概念、分类、研究现状及其发展前景;生物力学概念、研究现状及其研究方法。阐述了研究仿生学及生物力学应用的重要意义。  相似文献   

Control strategy for starting up of pure electric vehicle          下载免费PDF全文

HU Jianjun  DU Rui  JI Yi 《保鲜与加工》2013,(8):19-25

In order to solve the problem of large jerk and easy slipping down on the ramp in the starting process of the pure electric vehicle,starting up control strategy of pure electric vehicles is established on the basis of meeting the driver’s driving intentions. The starting process of pure electric vehicle is divided into mode without throttle opening and mode with throttle opening. And corresponding control strategies for the two modes are formulated respectively. The starting performance of pure electric vehicle adopting the control strategy is analyzed. The results show that the strategy satisfies the requirements of smoothness and safety well in the starting process of the pure electric vehicle.  相似文献   

Analysis on the export characteristics and internal flow of circumferential micro-turbines          下载免费PDF全文

TANG Gangzhi  ZHANG Li  DU Baocheng  LIAO Zhaobang 《保鲜与加工》2013,(7):1-5

Circumferential micro-turbine adapts to miniaturization as it has simple structure. To evaluate and improve the six-blade micro-turbine structure and analyze the influence of tip clearance on the flow, To simulate the output characteristics and gas flow characteristics, a three-dimensional transient numerical simulation model is set up and the output characteristics and gas flow characteristics are simulated. Results show that the six-blade turbine has structural defect. Parts of gas directly exhaust without working because intake port and exhaust have periodic connectivity while running. Simulation results for the improved eight-blade micro-turbine reveal that it hasnt such defects. Then, the effect to pressure drop of tip gap is simulated, and results show that the influence is nonlinear. As the gap is less than 0.01, the pressure drop is very sensitive to the gap. When gap is greater than 0.02, its impact is very small. Simulation and experimental results both show that the output mechanical power of the eight-blade turbine and six-blade turbine increases with the increase of gas flow. The eight-blade turbine mechanical power is 1 355 mW, which is increased 10.5% over the six-blade turbine. The power output start flow of eight-blade turbine is 140 L/h, while that of six-blade turbine is 200L/h. The performance of eight-blade turbine is better than that of six-blade turbine.  相似文献   

城镇化建设中工业遗产的保护与利用途径——以木鱼石矿区为例     

杜娟 《山东林业科技》2013,(6):56-58

随着时间的更迭与前进,城镇化建设中产生了大量面临淘汰或闲置的工业遗产．它们承载了各种不同的经验与文化累积,对于复兴城市经济活力、延续城市文脉有着极其重要的意义。但是这些具有历史意义与价值的对象有些面临淘汰,有些被保存的工业遗产,从保护的实质层面而言,则缺乏尊重文脉、注重可持续发展的理念。作者以木鱼石矿区为例,不仅关注有形的物质形态的保护与利用,还关注精神与历史记忆的保存,并从更全面地视角,提出了工业遗产保护与利用的途径,为城镇化建设中的工业遗产研究提供了新的思路和可能。  相似文献   

不同基质旱冬瓜扦插繁殖技术研究     

袁莲珍  史富强  杨斌  陈伟  刘际梅  张树红  李玉华 《四川林业科技》2013,(5):72-73,7

不同基质旱冬瓜扦插试验中,采用完全随机区组设计,在云南省林业科学院热带林业研究所（普文）扦插旱冬瓜在森林土、河沙和珍珠岩3种基质中,最适宜的为森林土,平均生根率为43.57％,平均生根数为6.06条,平均抽稍长度为4.26 cm,平均最长根长为9.39 cm.  相似文献