塔里木河流域生态环境恶化的水文效应   总被引：12，自引：9，他引：12  

程同福  张雄文  张洪  王建文  王立峰  段建军 《干旱区研究》2003,20(4):266-271

塔里木河是我国最大的内陆河。 4条源流出山口多年平均天然径流量 2 2 4 .9× 10 8m3 (195 7～ 2 0 0 1年 )。在全球变暖的大背景下 ,塔里木河流域山区气候出现变暖增湿的趋势 ,2 0世纪 90年代径流量达 2 4 1.9× 10 8m3 ,增幅 7.6 %。由于源流区人类开发利用水资源的影响 ,使得补给塔里木河干流的水量不断减少 ,水文条件改变 ,造成干流上、中游段耗水严重 ,导致下游生态与环境急剧退化。自 2 0 0 0年以来 ,实施的从开都河—博斯腾湖向下游绿色走廊应急输水 ,已使下游生态环境开始恢复生机。  相似文献   

20%多效唑·甲哌 FEE7 微乳剂防止小麦倒伏和增产机理研究   总被引：1，自引：0，他引：1  

段留生  李召虎  何钟佩  刁家连  田晓莉  王保民  贾敬业 《农药学学报》2002,4(4):33-39

冬小麦二棱期喷施植物生长调节剂20%多效唑·甲哌鎓微乳剂375 mL/hm2,可以显著抑制茎秆基部节间伸长,增加各节间充实度,其中赤霉素(Gas)和生长素(IAA)降低,可显著增强小麦抗倒伏能力和降低田间倒伏率.处理还协调了穗数、穗粒数和粒重的关系,增产幅度6.2%～28.6%.增产原因可能在于促进籽粒灌浆强,增加籽粒中内源Gas、IAA、细胞分裂素(CTKs)的水平,增强了籽粒库活性,同时促进茎叶中干物质向籽粒运转.  相似文献   

20%多效唑·甲哌Wong微乳剂防止小麦倒伏和增产机理研究   总被引：2，自引：0，他引：2  

段留生李召虎  何钟佩刁家连田晓莉  王保民贾敬业 《农药学学报》2002,4(4):33-39

冬小麦二棱期喷施植物生长调节剂20%多效唑·甲哌鎓微乳剂375 mL/hm2,可以显著抑制茎秆基部节间伸长,增加各节间充实度,其中赤霉素(Gas)和生长素(IAA)降低,可显著增强小麦抗倒伏能力和降低田间倒伏率.处理还协调了穗数、穗粒数和粒重的关系,增产幅度6.2%～28.6%.增产原因可能在于促进籽粒灌浆强,增加籽粒中内源Gas、IAA、细胞分裂素(CTKs)的水平,增强了籽粒库活性,同时促进茎叶中干物质向籽粒运转.  相似文献   

某些阳离子在大豆胞囊线虫生防系统中的作用   总被引：2，自引：0，他引：2  

邢丽娟  陈大力  刘维志  段玉玺 《中国生物防治》2002,18(1):1-5

室内测定了不同浓度的 6种阳离子 :钼、硼、锌、铜、锰和铁对大豆胞囊线虫卵的孵化、大豆种子发芽和根系生长 ,以及对线虫生防菌———厚垣轮枝菌菌丝生长的影响。结果发现 ,在供试所有浓度下 ,铜、锰和铁明显抑制大豆胞囊线虫的卵孵化 ,锌具明显刺激作用 ;在供试浓度水平较低时 ,铜、锰和铁对大豆种子发芽及根系生长有轻微抑制 ,锰的抑制较为明显 ,但均不影响进一步发育 ;在供试浓度较低时 ,铜、锰和铁对厚垣轮枝菌菌丝生长无任何影响。铜可作为线虫生防制剂的添加剂。  相似文献   

Influences of chronic hypoxia on the gene expression of Kv1.3,Kv2.1 and Kv3.1 induced by acute hypoxia in PASMC of rats     

HONG Zhi-gang  JIN Si  KONG Wei  WANG Di-xun  CHEN Qi-ling  SUN Bing-yong 《园艺学报》2002,18(10):1192-1195

AIM and METHODS: Total RNA was extracted from 6th rat subcultured pulmonary artery smooth muscle cells(PASMC) exposed to continual chronic hypoxia or normoxia and the effects of chronic hypoxia on the changes of Kv1.3,Kv2.1,Kv3.1 mRNA in cultured PASMC induced by acute hypoxia were studied by semiquantitative RT-PCR in vitro. RESULTS:①Kv1.3,Kv2.1,Kv3.1 genes were found to be expressed in PASMC of rats exposed either to hypoxia or normxia.②The expression of Kv2.1 and Kv3.1 in 6th subcultured of PASMC in normaxia group could be upregulated by exposure to acute hypoxia,the levels of Kv2.1 and Kv3.1 mRNA were significantly increased from 0.646±0.092, 0.782±0.104 to 1.059±0.134, 0.985±0.116,respectively (P<0.01,n=5). ③PASMC cultured continuously in chronic hypoxia for 6 subcultures and then exposed to normoxia for 12 h,thereafter the expression of Kv2.1 and Kv3.1 were downregulated by acute hypoxia for 6 hours.The level of Kv2.1 mRNA was significantly decreased from 1.008±0.117 to 0.649±0.097 (P<0.01,n=5). CONCLUSION:Kv2.1,Kv3.1 genes might be oxygen sensitive genes.Chronic hypoxia might change the response of these Kv genes of PASMC to acute hypoxia and down-regulate its expression,which might probably decrease the role of Kv in HPV.  相似文献   

二棘血蜱叮咬中华硬蜱纯化抗原免疫接种宿主后的交叉免疫反应     

刘志刚  叶炳辉  崔晓民  徐敏 《中国生物防治》2004,20(2):95-98

选用中华硬蜱105kD柱层析纯化抗原对新西兰兔进行免疫接种,再用二棘血蜱进行叮咬,并与单纯二棘血蜱叮咬组和佐剂对照组进行对比,以探讨不同种属硬蜱之间的交叉免疫抗性。结果显示:单纯二棘血蜱叮咬组吸血量为181 3±44 3mg,而二棘血蜱叮咬由中华硬蜱105kD纯化抗原免疫接种组和佐剂对照组新西兰兔后其吸血量分别为102 1±25 3mg和168 5±40 9mg,纯化抗原免疫接种组较单纯二棘血蜱叮咬组和佐剂对照组吸血量分别下降43 7%和39 4%(P<0 01)。同时,纯化抗原免疫接种组也较单纯二棘血蜱叮咬组和佐剂对照组产卵量有显著下降。该研究结果表明中华硬蜱和二棘血蜱之间存在着交叉免疫反应。  相似文献   

Roles of the Kv1.2, Kv1.5, Kv2.1 potassium channels in hypoxia pulmonary vasoconstriction     

DU Yi-mei  TANG Ming  LIU Chang-jin  HONG Zhi-gang  KE Qin-mei  DI Jiu-fang  LUO Hong-yan  HU Mou-xian  HU Xin-wu  XI Jiao-ya  TANG Bi  Jurgen Hescheler 《园艺学报》2004,20(9):1537-1541

AIM: To determine the role of Kv1.2, Kv1.5, Kv2.1 in the hypoxia pulmonary vasoconstriction (HPV). METHODS: Male Wistar rats were divided into two groups: normoxic group and hypoxic group. The single smooth muscle cell was obtained from pulmonary artery of Wistar rats with acute enzymatic digestion method. The conventional whole-cell patch clamp technique was used to record the resting membrane potential (Em) and the potassium currents of voltage-gated potassium channel (IKv) in rat pulmonary arterial smooth muscle cells (PASMC). Intracellular application of Kv1.2/Kv1.5/Kv2.1 antibodies (1∶125) was conducted through the whole-cell patch clamp system. RESULTS: ① Em of PASMC was depolarized after 24 h hypoxia compared with that of control cells . IKv of PASMC was decreased after 24 h hypoxia, . ② The mixture of Kv1.2/Kv1.5/Kv2.1 antibodies depolarized Em and inhibited IKv in PASMC from normoxic rat, whereas the mixture of Kir2.1/Kir2.3/Kir4.1 antibodies had no effects on them. ③ The mixture of Kv1.2/Kv1.5/Kv2.1 antibodies and the mixture of Kir2.1/Kir2.3/Kir4.1 antibodies had no effects on IKv and Em from rats hypoxic for 24 h. CONCLUSION: Kv1.2, Kv1.5, Kv2.1 might be oxygen sensitive potassium channels which mediated HPV.  相似文献   

Radioligand binding assay of insulin receptor in rabbit kidney during ischemic and reperfusion     

HE Tao  XIE Hua-fu  DUAN Cheng-gang  LI Rong-hui  FENG Zhi-qiang 《园艺学报》2004,20(5):866-869

AIM: To observe the change of insulin receptor in rabbit kidney with acute ischemic-reperfusion injury. METHODS: 15 Japanese white rabbits were allocated randomly into control group, ischemic-reperfusion group(IR group). IR group received clamping for 1 h followed by 2 h or 48 h of reperfusion. At 2 h or 48 h after reperfusion, glucose and insulin in serum were determined. Insulin receptor in renal tissue was analyzed by radioligand binging assay(BAD). RESULTS: The level of serum glucose increased after 2 h reperfusion in 2 groups, but in IR group the value increased much more higher than those in control groups(P＜0.05). Plasma insulin of IR group was significantly higher than that in control after 2 h reperfusion(P＜0.05). Scatchard analysis of data resulted in curvilinear profiles, indicating that there are two classes of receptors with different affinity or the presence of a single class of receptors with a negative cooperative hormone-receptor interaction. Data analyzed for a two-site model showed that the values of Bmax₁(high affinity site), Bmax₂(low affinity site) and Kd₁, Kd₂ were significantly lower than that of control (P＜0.05) after 2 h perfusion. 48 h after IR there was no difference of Bmax₁, Bmax₂, and Kd₁ between 2 groups,but Kd₂ of IR group was higher than that of control (P＜0.05). CONCLUSIONS: The results indicate that the effect of intrinsic insulin decreases in the progress of the renal ischemic-reperfusion. The resulting high serum glucose may aggravate renal injury in the progress of ischemic-reperfusion.  相似文献   

IFNγ诱导的p47GTPase对细胞内病原体的免疫     

段廷云  崔保安 《畜牧与兽医》2005,37(9):55-57

干扰素通过各种信号途径诱导它的效应基因转录和表达,这些被表达的蛋白质因子种类很多,它们通过不同的机制来发挥免疫作用。除了抗病毒、抗肿瘤及免疫调节作用以外,有些蛋白质因子还具有对细胞内寄生病原体的免疫作用。p47GTPase家族就是其中典型的一类,当其成员中的某个基因缺失时,动物表现出对相应病原体的易感性增强。p47GTPase通过与吞噬体结合来溶解病原微生物,并将病原体的抗原表位呈递给淋巴细胞,发挥多环节的免疫作用。  相似文献   

Effect of Luteolin on Blood Indexes,Liver and Kidney in Mice with Acute Mercury Poisoning     

TAN Xiao  YANG Da-qian  ZHANG Hai-li  JIANG Jing  CHEN Yan  ZHANG Zhi-gang 《中国畜牧兽医》2016,43(6):1506-1511

To study the effect of luteolin on blood indexes,liver and kidney in mice with acute mercury poisoning,28 mice were randomly divided into four groups:Control group (intraperitoneal injection 0.9% saline),luteolin group (lavage 100 mg/kg luteolin),mercuric chloride group (intraperitoneal injection 4 mg/kg mercury chloride) and mercury chloride+luteolin group (intraperitoneal injection 4 mg/kg mercuric chloride,lavage 100 mg/kg luteolin).The activities of ALT in serum,AST,CREA and BUN contents,blood WBC,RBC,HGB content and GSH and MDA contents of liver tissue were detected.Morphological changes of liver and kidney tissues were observed.The results showed that compared with the control group,the activities of ALT and AST of mercuric chloride group were extremely significantly increased (P < 0.01),serum CREA,BUN,blood WBC and liver tissue MDA contents were significantly increased (P < 0.05) while blood RBC,HGB and liver tissue GSH contents were significantly decreased (P < 0.05).Liver,kidney pathological changes were obviously.Compared with mercuric chloride group,the activities of ALT,AST in serum,CREA,BUN,blood WBC and liver tissue MDA contents of mercuric chloride+luteolin group were significantly decreased (P < 0.05),while blood RBC,HGB and liver tissue GSH contents were significantly elevated (P < 0.05).Liver,kidney pathological changes were attenuated obviously.The poisoning were characterized by inflammation and the occurrence of anemia when acute mercury poisoning occurred,liver and kidney showed different degrees of injury in mice.Luteolin could reduce the toxic effects of acute mercury poisoning on blood,liver and kidney.  相似文献