Determination of three-dimensional viscoelastic compliance in wood by tensile creep test     

Yusuke Taniguchi  Kosei Ando  Hiroyuki Yamamoto 《Journal of Wood Science》2010,56(1):82-84

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The effects of nitrogen uptake before and after heading on grain protein content and the occurrence of basal- and back-white grains in rice (Oryza sativa L.)     

Tadashi Tsukaguchi  Yae Taniguchi  Rie Ito 《Plant Production Science》2016,19(4):508-517

Chalky rice (Oryza sativa L.) grains are induced by high temperature during the grain-filling period. Plant nitrogen status also affects the occurrence of basal- and back-white grains (BBWG). The objective of this study was to elucidate the relations between nitrogen availability per spikelet during the grain-filling period (N_GF) and each of the percentage of BBWG and grain protein content (GPC). We further compared the effect of the components of N_GF determined before heading (N_BH) and after heading (N_AH) on BBWG and GPC. We grew the rice cultivar ‘Koshihikari’ in pots in 2012 and 2013, and top-dressed nitrogen at the panicle formation and heading stages, under two (2012) or three (2013) temperature regimes during the grain-filling period. GPC was explained well by N_GF, but BBWG was not. BBWG was best explained in a multiple-regression equation by mean air temperature after heading and by N_BH and N_AH. The partial correlation coefficients for N_BH were 1.6 and 3.0 times those for N_AH in 2012 and 2013, respectively. On the other hand, in a multiple-regression equation for GPC, the partial correlation coefficients for N_BH were 0.91 and 0.71 times those for N_AH in 2012 and 2013, respectively. These results suggest that rice grains are most sensitive to plant nitrogen status before heading for BBWG but after heading for GPC, and that there is an optimal timing for nitrogen top-dressing that would maximize the reduction in BBWG per unit increment of GPC.  相似文献   

Intercalated duct cells in the chicken pancreatic islet with special reference to the alloxan administration.     

K Mutoh  H Wakuri  K Taniguchi 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(5):493-496

The intercalated duct cells were observed in the A and B islets of the chicken pancreas. These cells adhered with each other by intercellular junctional complexes at the apical side. They had many microvilli projecting into the lumen. Abluminally, they displayed extended slender cytoplasmic processes between islet endocrine cells. Administration of alloxan resulted to denser cytoplasm and a more prominent thickening of cytoplasmic processes of the intercalated duct cells, although the blood glucose levels did not show appreciable changes by the treatment. The intercalated duct epithelial cells appeared clearly as stellate cells. The lysosomes increased in size and number with passage of time after alloxan administration. The present findings may suggest that intercalated ducts are not only anatomically important as a structure passing through the islet but also play physiologically by protecting the islet endocrine cells.  相似文献   

Quantitative PCR assay for the detection of the parasitic ciliate <Emphasis Type="Italic">Cryptocaryon irritans</Emphasis>     

Akito Taniguchi  Hiroyuki Onishi  Mitsuru Eguchi 《Fisheries Science》2011,77(4):607-613

We developed a quantitative PCR assay for detecting the parasitic ciliate Cryptocaryon irritans, which causes “white spot disease” in marine fishes, from the natural environment. A specific primer set for C. irritans was designed and its high specificity was confirmed in silico: almost all of the sequences deposited in the GenBank nucleotide database were covered, 22/23 for the forward primer and 7/7 for the reverse primer. We estimated that there were 3,415.9 rRNA gene copies per genome of C. irritans. In artificial mixture experiments to validate whether the qPCR assay is applicable to natural samples, the estimated copy numbers showed significantly positive correlations with the number of theronts added (p < 0.001). When we applied this qPCR assay to natural samples collected bimonthly from surface and bottom seawaters at an aquaculture site (water depth, 10 m) from May 2009 to March 2010, we only detected C. irritans (112.0 ± 6.3 cells/l) in the surface seawater sample in November. This qPCR assay is a useful tool for detecting C. irritans rapidly and quantitatively in natural environments; it could also help advance our understanding of the ecology of C. irritans, as well as facilitate the diagnosis of the disease.  相似文献   

Effects of Calcium Concentration in Potato Tuber Cells on the Formation of Cross-Links between Pectin Molecules by Ca<Superscript>2+</Superscript>     

Daiki Murayama  Masayuki Tani  Shinya Ikeda  Jiwan P. Palta  Samanthi W. Pelpolage  Hiroaki Yamauchi  Hiroshi Koaze 《American Journal of Potato Research》2017,94(5):524-533

The formation of cross-links between pectin molecules via Ca²⁺ in the potato tuber cell wall is a determinant factor on processing properties of potato and the quality of its products such as French fries. Thus, in this study, potato tubers varying significantly in their calcium concentrations were analyzed to investigate whether an increased absorption of calcium by a potato tuber led to an increase in the calcium concentration in the cell wall and how the calcium concentration in the cell wall influenced on the formation of cross-links between pectin molecules via Ca²⁺. Correlation analysis revealed that calcium absorbed by a potato tuber was bound to the cell wall as a water insoluble form 99 days after planting or later. Furthermore, with an increase in the calcium concentration in the cell wall, the content of chelator soluble pectin increased throughout tuber bulking and maturation stages. However, the degree of methylation was not a limiting factor in the formation of cross-links between pectin chains via Ca²⁺. Atomic force microscopy images of parenchyma cell walls prepared from mature potato tubers indicated an increase in the amount of calcium cross-linked pectin molecules with an increase in the calcium concentration in the cell wall. The present study demonstrated that the calcium concentration of the cell wall of potato tubers significantly affected the formation of cross-linkages between pectin molecules and, consequently, contributed to an enhanced formation of pectin-calcium networks in the cell wall.  相似文献   

Repeated restraint stress modifies fatty acid and amino acid metabolism in the mouse skin     

Yume KITAGAWA  Kaho HAYAKAWA  Daichi OIKAWA  Kazuki IKEDA  Maki IKEDA  Daiki HARADA  Mitsuhiro FURUSE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(4):511

In modern society, stress caused by relationships and emotions is one of the greatest social problems. Similar to humans, domestic and captive animals live under various stresses. Several stresses have been associated with skin disorders, such as atopic dermatitis, but there is a lack of reliable and objective indicators for the characterization of this association. This study aimed to define the changes in fatty acid composition and amino acid concentration in the skin following repeated restraint stress in ICR mice. Mice subjected to 30 min of daily restraint stress for 8 days showed changes in the composition of saturated fatty acids, such as an increase in palmitic acid content, which are the substrates of Δ-9 desaturase. Conversely, unsaturated fatty acids decreased with stress treatment, which appeared to be a result of these fatty acids being the substrate of Δ-6 desaturase. Changes in fatty acid composition after stress treatment may be one of the factors that cause skin inflammation. The water-retention capacity may have been lowered by stress treatment because histidine and leucine, which are natural moisturizing factors, were significantly decreased. The collagen content in the skin gradually decreased after repeated stress treatment. Our results indicate that repeated restraint stress may impact skin health through changes in both the fatty acid composition and amino acid concentration in mice.  相似文献   

Retrospective study of porcine circovirus 2 infection in Japan: seven cases in 1989     

Mori M  Sato K  Akachi S  Asahi S  Taniguchi S  Narita M 《Veterinary pathology》2000,37(6):667-669

Retrospectively, we demonstrated that seven pigs necropsied in 1989 had characteristic porcine circovirus 2 (PCV-2) lymphoid tissue lesions of severe lymphoid depletion, syncytial giant cell formation, and intracytoplasmic inclusion bodies in macrophages. Immunohistochemically, PCV-2 antigen was detected in these tissues and the distribution of positive staining corresponded to the distribution of inclusion bodies. Electron microscopy demonstrated viral particles compatible with porcine PCV-2. Therefore, the disease occurred in Japan as early as 1989.  相似文献   

Immunohistochemical studies on the development of endocrine cells in the thyroid and parathyroid glands in the golden hamster     

K Taniguchi  Y Shutoh  S Mikami 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(1):19-27

The development of endocrine cells in the thyroid and parathyroid glands in the golden hamster was studied immunohistochemically in relation to the formation of these glands. The thyroid was formed on day 9 of gestation by the ventral outpocketing of the foregut between the first and second branchial pouches. The thyroid epithelial cells were faintly thyroglobulin-immunoreactive on day 10.5 of gestation. This immunoreaction became intense thereafter, but was almost confined to the cytoplasm of epithelial cells until birth. It appeared in the follicular lumen in newborn animals. The ultimobranchial body was derived from the fifth pouch and fused with the thyroid on day 12 of gestation. Calcitonin-immunoreactive cells first appeared on day 14 of gestation in the dorsomedial part of the thyroid derived from the ultimobranchial body and increased in number and intensity thereafter. Somatostatin-immunoreactive cells also appeared in the dorsomedial part of the thyroid derived from the ultimobranchial body on day 13 of gestation, and increased in number in newborn animals, but decreased thereafter. The parathyroid was derived from the third pouch, situated on day 13 of gestation on the dorsolateral side of the thyroid, and surrounded by a common capsule with the thyroid. Parathormone-immunoreactive cells first appeared on day 15 of gestation in the parathyroid and increased in number and intensity after birth.  相似文献   

Preparation and characterization of neutralizing monoclonal antibodies to feline calicivirus.   总被引：2，自引：0，他引：2  

Y Tohya  Y Taniguchi  M Tsubakimoto  E Takahashi  T Mikami 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(2):251-256

Fourteen neutralizing monoclonal antibodies (N-MoAbs) were prepared against the F4 strain of feline calicivirus (FCV), the prototype strain of FCV in Japan, and examined for their ability to neutralize FCV isolates. Neutralization-resistant variants of the F4 strain were selected under the presence of 4 individual N-MoAbs in cell culture systems and used in cross-neutralization tests and enzyme-linked immunosorbent assay with all of the 14 N-MoAbs. The results revealed the identification of at least two antigenic determinants on FCV F4: one being more broadly conserved among FCV isolates than the other. Usefulness of antigenic variants resistant to N-MoAbs for analysis of neutralization determinants on FCV was also demonstrated.  相似文献   

Quantitative and morphological studies on the influence of zinc deficiency on the liver of pregnant rats     

K Mamba  K Taniguchi  S Kagabu  T Makita 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1989,51(3):566-573

The effect of zinc deficiency on trace metals in the liver, spleen, kidney, pancreas and duodenum was investigated in the control and zinc-deficient rats at 17 days and 20 days of pregnancy. Zinc-deficient rats fell into limosis after 5 days of pregnancy. The contents of zinc, iron, copper and manganese in the maternal tissues were measured by colorimetry with 5NPPF. The morphological changes of the liver were observed by light and electron microscopy. The contents of zinc in the pancreas and duodenum were less in the zinc-deficient group than in the control at both 17 days and 20 days of pregnancy. The contents of the copper and manganese in the liver, kidney, pancreas, duodenum and spleen, however, were not significantly different from the control. The contents of iron in the liver, spleen and kidney in the deficient group increased greatly at 17 days and 20 days of pregnancy compared with the control group. The combination rate of transferrin with iron also increased significantly in the deficient group. Staining with Berlin blue or Turnbull's blue showed intense reaction to iron around the interlobular connective tissue of the liver in the zinc deficient group at 20 days of pregnancy. Ultrastructurally, the liver of the zinc-deficient rats showed the decrease of glycogen granules and increase of lipid-like granules and lysosomes with various sizes and electron densities. These findings suggest that zinc deficiency causes the increase of iron contents in the various organs during pregnancy, and that there is an intimate interrelationship between zinc and iron in the metabolism of iron during pregnancy.  相似文献