Detection of indoleamine 2,3-dioxygenase 1-expressing cells in canine normal and tumor tissues     

Namiko IKEDA  Daiki KATO  Masaya TSUBOI  Ryohei YOSHITAKE  Shotaro ETO  Sho YOSHIMOTO  Masahiro SHINADA  Satoshi KAMOTO  Yuko HASHIMOTO  Yousuke TAKAHASHI  James CHAMBERS  Kazuyuki UCHIDA  Ryohei NISHIMURA  Takayuki NAKAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(12):1885

Cancer immunotherapy is a novel cancer treatment for canine tumors. Indoleamine 2,3-dioxygenase 1 (IDO1) is overexpressed in some human tumors and inhibits antitumor immunity. In this study, we comprehensively evaluated expression pattern of IDO1 and the nature of IDO1-expressing cells in canine normal and tumor tissues. In normal tissue samples, IDO1 expression was detected only in the lymph nodes, spleen, tonsil tissues, and colon tissues. In contrast, IDO1-positive tumor cells were observed in several tumor tissue types. This is the first study to evaluate IDO1 expression in canine normal and tumor tissues, and the results suggest that IDO1 is a promising target for novel cancer immunotherapy in dogs with tumors.  相似文献   

Common nucleotide sequence of structural gene encoding fibroblast growth factor 4 in eight cattle derived from three breeds     

Sho SATO  Toshikiyo TAKAHASHI  Hiroshi NISHINOMIYA  Makiko KATOH  Ryu ITOH  Masaki YOKOO  Mari YOKOO  Momoe IHA  Yuki MORI  Kano KASUGA  Ikuo KOJIMA  Masayuki KOBAYASHI 《Animal Science Journal》2012,83(3):260-262

Fibroblast growth factor 4 (FGF4) is considered as a crucial gene for the proper development of bovine embryos. However, the complete nucleotide sequences of the structural genes encoding FGF4 in identified breeds are still unknown. In the present study, direct sequencing of PCR products derived from genomic DNA samples obtained from three Japanese Black, two Japanese Shorthorn and three Holstein cattle, revealed that the nucleotide sequences of the structural gene encoding FGF4 matched completely among these eight cattle. On the other hand, differences in the nucleotide sequences, leading to substitutions, insertions or deletions of amino acid residues were detected when compared with the already reported sequence from unidentified breeds. We cannot rule out a possibility that the structural gene elucidated in the present study is widely distributed in cattle. To the best of our knowledge, this is the first determination of the complete nucleotide sequence of the structural gene encoding bovine FGF4 in identified breeds.  相似文献   

Progresses and insights of Fas-associated factor 1     

DONG Shao-nan  XIE Qi-xuan  AKIYAMA Taishin  TAKAHASHI Yuji  QIN Jun-wen 《园艺学报》2012,28(3):565

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Efficacy of leflunomide for treatment of refractory inflammatory colorectal polyps in 15 Miniature Dachshunds     

Kenjiro FUKUSHIMA  Nozomi EGUCHI  Koichi OHNO  Hideyuki KANEMOTO  Masashi TAKAHASHI  Hirotaka IGARASHI  Aki OHMI  Ko NAKASHIMA  Hajime TSUJIMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):265-269

Inflammatory colorectal polyp (ICRP), common in miniature dachshunds, presents with hematochezia, tenesmus and mucoid feces. Although an 80% response rate has been reported when treated with prednisolone and cyclosporine, effective treatment is needed for the remaining 20% of ICRP dogs. Leflunomide is an immunosuppressive drug reported as effective in several immune-mediated diseases. In the present study, we retrospectively evaluated the efficacy and adverse effects of leflunomide in 15 ICRP dogs that were refractory to treatment with prednisolone and cyclosporine. Treatment efficacy was assessed by endoscopy, clinical symptoms and rectal palpation. Adverse effects were determined by clinical symptoms and blood testing during follow-up. The leflunomide treatment response rate was 93.3%. The median dosage of leflunomide and the median response time were 3 mg/kg (range: 1.7–4.0 mg/kg) and 35 days (range: 20–119 days), respectively. Adverse effects observed included lethargy (3 dogs), anorexia (1 dog), respiratory symptoms (1 dog), leukocytopenia (2 dogs), thrombocytopenia (1 dog), anemia (1 dog) and liver enzyme elevation (8 dogs). Most of the adverse effects improved with symptomatic treatment and leflunomide discontinuation or dosage reduction. In conclusion, leflunomide treatment is effective in ICRP dogs refractory to treatment with prednisolone and cyclosporine. Because several adverse effects were observed, close monitoring is needed during leflunomide treatment follow-up.  相似文献   

Expression and localization of epidermal growth factor,transforming growth factor-α and epidermal growth factor receptor in the canine testis     

Hiromichi TAMADA  Kohei TAKEMOTO  Masato TOMINAGA  Noritoshi KAWATE  Masahiro TAKAHASHI  Shingo HATOYA  Satoshi MATSUYAMA  Toshio INABA  Tsutomu SAWADA 《The Journal of reproduction and development》2016,62(1):59-64

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Regression of viral pathogen indicators due to improvement of hygiene protocols on boots in a bovine farm     

Satoru TAKAHASHI  Md. Amirul HASAN  Mariko ITO  Miyuki KOMURA  Chisato DAIO  Mizuki ONO  Makiko YAMAGUCHI  Md. Shahin ALAM  Md. Humayun KABIR  Yu MIYAOKA  Dany SHOHAM  Kazuaki TAKEHARA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2020,82(12):1793

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Cardiorespiratory and anesthetic effects of combined alfaxalone,butorphanol, and medetomidine in Thoroughbred horses     

Hajime OHMURA  Atsushi OKANO  Kazutaka MUKAI  Kentaro FUKUDA  Toshiyuki TAKAHASHI 《Journal of Equine Science》2016,27(1):7-11

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Aluminum status of synthetic Al–humic substance complexes and their influence on plant root growth     

Tadashi TAKAHASHI  Masami NANZYO  Syuntaro HIRADATE 《Soil Science and Plant Nutrition》2007,53(2):115-124

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Comparison of the depth of placement of lime nitrogen on growth, N₂ fixation activity, seed yield and quality of soybean (Glycine max (L.) Merr.) plants     

Tewari KAUSHAL  Masaru ONDA  Sayuri ITO  Akihiko YAMAZAKI  Hiroyuki FUJIKAKE  Norikuni OHTAKE  Kuni SUEYOSHI  Yoshihiko TAKAHASHI  Yoshifumi NAGUMO  Tohru TSUCHIDA  Takuji OHYAMA 《Soil Science and Plant Nutrition》2006,52(4):453-463

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Conversion of soil particle size distribution and texture classification from ISSS system to FAO/USDA system in Japanese paddy soils     

Tomoki TAKAHASHI  Keiko NAKANO  Rikiya NIRA  Etsushi KUMAGAI  Mizuhiko NISHIDA  Mari NAMIKAWA 《Soil Science and Plant Nutrition》2020,66(3):407-414

ABSTRACT

The conversion between the two different systems, ISSS and FAO/USDA, of particle size distribution and soil texture classification is useful to characterize soil physical properties and usage of each published. The objective of this study is to test some functions that have been published for conversion from ISSS to FAO/USDA system for Japanese paddy soils and to select the best method. We tested the topsoils of 267 Japanese paddy fields using the log-linear method, log-normal method, multiple regression method, Skaggs et al.’s method, and Minasny and McBratney’s method. The least AIC was obtained using multiple regression method, and the equation derived was given as follows:

si_FAO/USDA = 1.305si_ISSS + 0.396fs_ISSS?0.100cs_ISSS ? 12.323

where si, fs, and cs are the percentage of silt, fine sand, and coarse sand respectively; ISSS and FAO/USDA is the fractionation system; and its RMSE was 3.1%. For the case that only the total sand content (s) is available instead of fine sand and coarse sand, the following equation was obtained:

si_FAO/USDA = 0.314si_ISSS + 1.533s_ISSS ? 20.903 (RMSE = 3.7%)

Among the non-empirical methods, the best estimation method was Skaggs et al.’s method, and its RMSE was 3.3%. The soil texture classification by FAO/USDA system using estimated particle size fractions by the above equation can be classified to correct categories. The accuracy ratio of the classification was 93-97%.  相似文献