Positive correlation between renal tubular flattening and renal tubular injury/interstitial fibrosis in murine kidney disease models     

Yuki TAKAHASHI  Masaki WATANABE  Koki HIURA  Ai ISOBE  Hayato SASAKI  Nobuya SASAKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(3):397

The number of patients with chronic kidney disease (CKD) is growing continuously globally. In order to study pathogenesis and mechanisms, many animal models have been developed, including spontaneous, genetic, and induced models. Although each type of CKD shows disease-specific tissue changes in the early stages, tubular disorder and interstitial fibrosis histologically occur in the course of progression to end-stage renal failure. Therefore, the quantification of tubular disorder and interstitial fibrosis in CKD research using animal models is essential for measuring the degree of CKD severity and, thus, efficacy of therapeutic agents. Several strategies have been used to quantify interstitial fibrosis. Among scoring factors, renal tubular flattening can be quantitatively evaluated easily and inexpensively. However, the diagnostic value of renal tubular flattening evaluation has not been investigated previously. Therefore, in this study, we investigated the correlation between renal tubular flattening and interstitial fibrosis or renal tubular injury markers. We observed a strong correlation between the degree of tubular injury/interstitial fibrosis and renal tubular flattening in three types of mouse renal disease model. This is advantageous because rapidly advancing technologies such as artificial intelligence and image processing can be easily applied; hence, a more precise, objective, and quantitative diagnosis should be possible in the future.  相似文献   

Properties of a virus inactivator from yeast     

TAKAHASHI WN 《Science (New York, N.Y.)》1946,104(2703):377

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Successful outcome after a single endoscopic fecal microbiota transplantation in a Shiba dog with non-responsive enteropathy during the treatment with chlorambucil     

Koji SUGITA  Ayaka SHIMA  Kaho TAKAHASHI  Yasuyoshi MATSUDA  Masaki MIYAJIMA  Marin HIROKAWA  Hirotaka KONDO  Junpei KIMURA  Genki ISHIHARA  Keitaro OHMORI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):984

A 7-year 6-month-old, castrated male Shiba dog presented with a 1-month history of lethargy, anorexia, vomiting, and frequent watery diarrhea. Weight loss, hypoalbuminemia, anemia, and leukocytosis were detected at the first visit. The dog was diagnosed with non-responsive enteropathy (NRE) based on clinical and histopathological examinations. Since the dog did not respond to the immunosuppressive drugs, fecal microbiota transplantation (FMT) was performed during the treatment with chlorambucil. A single endoscopic FMT into the cecum and colon drastically recovered clinical signs and clinicopathological abnormalities and corrected dysbiosis in the dog. No recurrence or adverse events were observed. The present case report suggests that FMT, possibly together with chlorambucil, might be a treatment option for NRE in Shiba dogs that have poorer prognosis compared with other dog breeds.  相似文献   

Effect of chlorate on nitrification in longan plantation soil     

Pathipan SUTIGOOLABUD  Takafumi MIZUNO  Somchai ONGPRASERT  Shuichi KARITA  Toru TAKAHASHI  Hitoshi OBATA  Keishi SENOO 《Soil Science and Plant Nutrition》2008,54(3):387-392

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Expression of podoplanin in various types of feline tumor tissues     

Satoshi KAMOTO  Masahiro SHINADA  Daiki KATO  Masaya TSUBOI  Sho YOSHIMOTO  Ryohei YOSHITAKE  Shotaro ETO  Namiko IKEDA  Yosuke TAKAHASHI  Yuko HASHIMOTO  James CHAMBERS  Kazuyuki UCHIDA  Shinji YAMADA  Mika K. KANEKO  Ryohei NISHIMURA  Yukinari KATO  Takayuki NAKAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1795

Podoplanin is expressed in various human tumors where it promotes tumor progression, epithelial-mesenchymal transition, and distant metastasis. Podoplanin is also expressed in cancer-associated fibroblasts and induces tumor malignancy. The objective of this study was to evaluate podoplanin expression in various types of feline tumor tissues. Immunohistochemical analysis revealed that podoplanin was expressed in cells of 13/15 (87%) squamous cell carcinomas and 5/19 (26%) fibrosarcomas. Moreover, cancer-associated fibroblasts expressed podoplanin in most tumor types, including 18/21 (86%) mammary adenocarcinoma tissues. Our findings demonstrate that various types of feline tumor tissues expressed podoplanin, indicating the importance of the comparative aspects of podoplanin expression, which may be used as a novel research model for podoplanin biology.  相似文献   

Increasing nitrate removal at low temperatures by incorporating organic matter into paddy fields   总被引：1，自引：0，他引：1  

Tomoki TAKAHASHI  Hidehiro INAGAKI  Tsutomu FUKUSHIMA  Tomohiro OISHI  Kazuo MATSUNO 《Soil Science and Plant Nutrition》2010,56(1):163-167

Denitrification of paddy fields is a key process for improving water quality in fields where nitrate concentrations are high. The objective of the present study was to understand the effects of incorporating organic carbon (C) into soil on the denitrification rate of paddy fields in winter. On 11 December 2007, separate paddy field plots were prepared by incorporating 5 Mg ha⁻¹ of rice straw (RS), 11 Mg ha⁻¹ of rice straw compost (RSC) or a control. A field with a high concentration of nitrate in the water (averaging 18 mg N L⁻¹) was irrigated until 29 March. During the experiment, the daily average soil temperature at a depth of 0.05 m ranged between 3 and 15°C. The nitrate concentration in the surface water in the RS plot, where the residence time was 2 days, decreased more than the concentration in the control or RSC plots. The total estimated nitrate removal from each plot in relation to the other plots was RS > RSC = control. Measurements of the soil from each plot on 29 February 2008 showed that incorporation of RS significantly increased the denitrification potential, even at low temperatures (5–10°C). Furthermore, the RS plot contained more dissolved organic C than the control or RSC plots. This result indicates that supplying RS effectively increases denitrification under low-temperature conditions.  相似文献   

聚丙烯酰胺凝胶电泳快速检测大豆过敏原蛋白Gly m Bd30K缺失方法     

王绍东  李远明  刘伟  姜妍  王浩  于佳  李文滨  高桥浩司 《大豆科学》2011,30(4):638-642

通过对现有SDS-PAGE电泳技术的样本前处理和制胶技术的改良,开发出了一种能够同时清晰鉴别7S球蛋白组分亚基(α',α,β-Subunit)和Gly m Bd 30 K谱带的SDS-PAGE电泳快速检测新技术.利用该方法对PI603570A×0329F6杂交组合F2群体的116个样本进行了7S球蛋白组分亚基(α',α...  相似文献