First report of <Emphasis Type="Italic">Rhizoctonia</Emphasis> disease of lily caused by <Emphasis Type="Italic">Rhizoctonia solani</Emphasis> AG-11 in Japan     

Tomoo?MisawaEmail authorView author&#;s OrcID profile  Miyuki?Kayamori  Daisuke?Kurose  Jun?Sasaki  Takeshi?Toda 《Journal of General Plant Pathology》2017,83(6):406-409

Blight on leaves, stems and bulbs of lilies grown in a greenhouse were found in Hokkaido, Japan, in 2012. Two isolates obtained from the lesions were identified as Rhizoctonia solani anastomosis group (AG)-11 based on morphology and molecular analysis. Original symptoms were reproduced after artificial inoculation with the isolates. Except for R. solani AG-2-1 and AG-4 HG-I, none of the AGs have been reported as pathogens causing lily Rhizoctonia disease in Japan; therefore, we propose adding AG-11 as a pathogen of the disease. More importantly, we report the first appearance of crop disease caused by AG-11 in Japan.  相似文献   

Enantiomeric analysis of monoterpenes in Oba-kuromozi (<Emphasis Type="Italic">Lindera umbellata</Emphasis> var. membranacea)     

Risa Inoue  Koetsu Takahashi  Yoshiaki Iiduka  Daisuke Arai  Tatsuya Ashitani 《Journal of Wood Science》2018,64(2):164-168

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Characterization of Japanese <Emphasis Type="Italic">Rhizoctonia solani</Emphasis> AG-2-1 isolates using rDNA-ITS sequences,culture morphology,and growth temperature     

Tomoo Misawa  Daisuke Kurose  Manami Mori  Takeshi Toda 《Journal of General Plant Pathology》2018,84(6):387-394

Rhizoctonia solani AG-2-1 is classified into three subsets (Subsets 1–3) based on the rDNA-ITS sequence. Few Japanese isolates, however, have been phylogenetically analyzed. To understand the distribution and diversification of AG-2-1 isolates in Japan, we examined 23 Japanese AG-2-1 isolates (15 from Hokkaido, the northernmost island, and eight from NARO Genebank) in terms of rDNA-ITS sequences, culture morphology, and temperature-dependent growth characteristics. Of these, 15 isolates were found to belong to Subset 1. One isolate, which formed a light brown colony with concentric rings and grew slowly at 25 °C, was classified into Subset 2. Six isolates had varied culture morphology and relatively faster growth than Subset 1 isolates at 30 °C. They formed a clade on the phylogenetic tree, designated clade HK, with cauliflower isolates from Belgium and the Netherlands, with a bootstrap value of 47%, and were separate from the three known subsets. Sequence similarity in the rDNA-ITS region for this clade ranged from 98.2 to 100%, whereas clade HK isolates had 96.7–98.6% similarity with the isolates in each subset. This result suggests that clade HK is likely an independent intragroup within AG-2-1, although the rDNA-ITS sequences in this clade were variable. One isolate was not assignable to any clade because it was intermediate between isolates in clade HK and Subset 2. This is the first report describing variation among rDNA-ITS sequences of Japanese AG-2-1 isolates.  相似文献   

Inverse regulation of leptin mRNA expression by short- and long-chain fatty acids in cultured bovine adipocytes     

Soliman M  Kimura K  Ahmed M  Yamaji D  Matsushita Y  Okamatsu-Ogura Y  Makondo K  Saito M 《Domestic animal endocrinology》2007,33(4):400-409

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Structural weakening of intramuscular connective tissue during postmortem aging of pork     

Takanori NISHIMURA  Suhong FANG  Toshiaki ITO  Jun‐ichi WAKAMATSU  Koui TAKAHASHI 《Animal Science Journal》2008,79(6):716-721

We studied structural changes in the endomysium and perimysium during postmortem aging of pork using the cell‐maceration/scanning electron microscope method. Immediately post mortem, endomysia sheaths that house individual muscle fibers displayed a honeycomb‐like structure. The sheaths of the endomysium consisted of tightly arranged collagen fibrils in a random network. The perimysium comprised several layers of wavy sheets made up of tightly bundled collagen fibers. While the structure of the intramuscular connective tissues remained almost unchanged up to five days post mortem, the endomysium had resolved into individual collagen fibrils, and the thick sheets of the perimysium had separated into collagen fibers and fibrils at 8 days post mortem. These results provide direct evidence for structural weakening of the endomysium and perimysium during postmortem aging of pork. The shear‐force value of raw pork decreased rapidly within six days post mortem and then decreased slowly until 14 days post mortem. Since the rapid increase in tenderness is mainly due to structural weakening of myofibrils, we conclude that the disintegration of the endomysium and perimysium contributes to tenderization of pork during extended postmortem aging.  相似文献   

Phenotypic and genotypic characterization of Actinobacillus suis sensu stricto isolated from a dairy calf     

Michika ISHIHARA  Yuka YAMAZAKI  Ken KATSUDA  Hiroya ITO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(5):624

The species of the genus Actinobacillus have so far been associated with specific animal hosts, and A. suis sensu stricto, an opportunistic pathogen of swine, is rarely isolated from ruminants. We describe here the isolation of A. suis sensu stricto from a newborn calf that died on a dairy farm in Japan. Identification of the isolate was performed by phenotypic and genotypic characterization, with the latter consisting of nucleotide sequence analyses of the 16S rRNA gene plus three housekeeping genes, rpoB, infB and recN.  相似文献