Real-time imaging of nitrogen fixation in an intact soybean plant with nodules using ¹³N-labeled nitrogen gas     

Satomi ISHII  Nobuo SUZUI  Sayuri ITO  Noriko S. ISHIOKA  Naoki KAWACHI  Norikuni OHTAKE  Takuji OHYAMA  Shu FUJIMAKI 《Soil Science and Plant Nutrition》2009,55(5):660-666

Real-time images of nitrogen fixation in an intact nodule of hydroponically cultured soybean ( Glycine max [L] Merr.) were obtained. In the present study, we developed a rapid method to produce and purify ¹³N-labeled radioactive nitrogen gas (half life: 9.97 min). ¹³N was produced from a ¹⁶O (p, α) ¹³N nuclear reaction. The target chamber was filled with CO₂ and irradiated for 10 min with protons at an energy of 18.3 MeV and an electric current of 5 μA, which was delivered from a cyclotron. All CO₂ in the collected gas was absorbed and removed with powdered soda-lime in a syringe and replaced with helium gas. The resulting gas was injected into gas chromatography and separated and a 35 mL fraction, including the peak of [¹³N]-nitrogen gas, was collected by monitoring the chromatogram. The obtained gas was mixed with 10 mL of O₂ and 5 mL of N₂ and used in the tracer experiment. The tracer gas was fed into the underground part of intact nodulated soybean plants and serial images of the distribution of ¹³N were obtained non-invasively using a positron-emitting tracer imaging system (PETIS). The rates of nitrogen fixation of the six test plants were estimated to be 0.17 ± 0.10 μmol N₂ h⁻¹ from the PETIS image data. The decreasing rates of assimilated nitrogen were also estimated to be 0.012 ± 0.011 μmol N₂ h⁻¹. In conclusion, we successfully observed nitrogen fixation in soybean plants with nodules non-invasively and quantitatively using [¹³N]N₂ and PETIS.  相似文献   

Effects of a combination of hinokitiol (β‐thujaplicin) and an organic acid mixture on ruminal fermentation in heifers fed a high‐grain diet     

Junichiro ISHII  Hiroshi OMURA  Tadao MITSUI  Norichika EGUCHI  Takashi UENO  Hisaya GOTO  Hiroshi ITO 《Animal Science Journal》2012,83(1):36-42

This study evaluated the effects of hinokitiol (a natural antibacterial compound extracted from Thujopsis dolabrata var. hondai) and an organic acid mixture (citrate content 50%) on ruminal fermentation. Antibacterial properties were examined by measuring minimal inhibitory concentration. Hinokitiol at 1.56 µg/mL or an organic acid mixture at 1600 µg/mL inhibited Streptococcus bovis growth. The combination of 0.78 µg/mL hinokitiol and 200 µg/mL of an organic acid mixture also inhibited S. bovis growth. Both hinokitiol and the hinokitiol and an organic acid mixture combination showed strong antibacterial properties on Gram‐positive bacteria such as S. bovis, but relatively weak antibacterial activities on Gram‐negative bacteria such as Megasphaera elsdenii. Three ruminally cannulated heifers were fed a bloat‐producing diet containing barley, pelleted alfalfa meal, soybean meal and salt without long‐cut roughage to investigate the ruminal characteristics in vivo. Feeding to heifers a bloat‐producing diet containing 7.8 mg/kg hinokitiol and 0.2% of an organic acid mixture significantly decreased the increase in stable ingesta volume. Hinokitiol or an organic acid mixture did not affect ruminal volatile fatty acids, protozoa and bacteria. These results suggest that a combination of hinokitiol and an organic acid mixture might reduce frothy bloat in cattle fed high‐grain diets.  相似文献   

A Case of Meconium Aspiration Syndrome in a Bottlenose Dolphin (Tursiops truncatus) Calf     

Miyuu TANAKA  Takeshi IZAWA  Mitsuru KUWAMURA  Miki OZAKI  Tatsuko NAKAO  Shu ITO  Jyoji YAMATE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(1):81-84

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THE THEORETICAL ANALYSIS OF RADIAL DISPLACEMENT AND COMPATIBLE CONDITIONS OF BOTTOM PLATE OF PISTON FRAME IN LARGE CONTAINER     

Wang Daiyu Sun Renbo 《保鲜与加工》1990,(1)

Taking aecount of shear action between the piston truss the bottom plate, this paper assumes the bottom plate to be a part-ring and supposes that the shear distribution follows the power series law, making the problem to be a problem of mixed boundary condition of part plate. In the paper, the solution of this elastic plane problem is derived. The general expressions of radial displacement of bottom plate are given. The compatible conditions both at the nodes of the lower chord of the truss and at the central cylindrical shell are analyzed.  相似文献   

ANALYTICAL SOLUTION FOR LATERAL BENDING OF RECTANGULAR STIFFENED PLATES CONSIDERING THE EFFECT OF STRAIN IN THE MIDDLE PLANE     

Sun Renbo  Wang Daiyu 《保鲜与加工》1989,(2)

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The genetic organization of the capsular polysaccharide biosynthesis region of Actinobacillus pleuropneumoniae serotype 15     

Hiroya ITO  Masuo SUEYOSHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):483-486

Nucleotide sequence determination and analysis of the cps gene involved in the capsular polysaccharide biosynthesis of Actinobacillus pleuropneumoniae serotype 15 revealed the presence of three open reading frames, designated as cps15ABC genes. At the protein level, Cps15A and Cps15B showed considerably high homology to CpsA (67.0 to 68.7%) and CpsB (31.7 to 36.8%), respectively, of A. pleuropneumoniae serotypes 1, 4 and 12, revealing the common genetic organization of the cps among serotypes 1, 4, 12 and 15. However, Cps15C showed no homology to any proteins of A. pleuropneumoniae serotypes, indicating that cps15C may be specific to serotype 15. This study will provide the basic molecular knowledge necessary for the development of diagnostics and a vaccine for A. pleuropneumoniae serotype 15.  相似文献   

Detection of rickettsial DNA in ticks and wild boars in Kyoto City, Japan     

Azusa SOMEYA  Ryuki ITO  Akihiko MAEDA  Mitsuhiro IKENAGA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):37-43

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Hyperferritinemia is associated with short survival time in dogs with multicentric lymphoma     

Seishiro CHIKAZAWA  Yasutomo HORI  Fumio HOSHI  Kazutaka KANAI  Naoyuki ITO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):843-846

In the present study, we examined the relationship between serum ferritin concentration before treatment and survival time in dogs with multicentric lymphoma. Eighteen dogs with multicentric lymphoma were enrolled in the study. When the dogs were classified into high and low ferritin groups on the basis of their serum ferritin concentration (3,000 ng/ml cut-off value), the median survival time of dogs with high concentrations (≥3,000 ng/ml, n=7) was 40 days, whereas it was 360 days among dogs with low concentrations (<3,000 ng/ml, n=11). This difference was statistically significant (P=0.001). This finding suggests that the initial high level of serum ferritin indicates short survival time in dogs with multicentric lymphoma. Large-scale research is necessary to confirm this finding.  相似文献   

Dark adaptation time in canine electroretinography using a contact lens electrode with a built-in light source     

Seiya MAEHARA  Yoshiki ITOH  Sho HOSHINO  Miri HAYASHI  Yosuke ITO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1335-1338

The purpose of this study was to evaluate the dark adaptation time in canine electroretinography (ERG) using a contact lens electrode with a built-in LED. Twelve eyes of six normal laboratory beagle dogs were used and exposed to steady room light at 500 lux for 30 min for light adaption. ERG was recorded at different time points during dark adaptation in sedated and light-adapted beagles. The stimulus intensity was 0.0096 cd/m²/sec. The b-wave amplitude increased significantly until 25 min of dark adaptation, whereas no significant changes in amplitudes were observed after 30 min. Dark adaptation for more than 25 min would be necessary for accurate ERG in canine ERG using a contact lens electrode with a built-in LED.  相似文献   

耐性植物秸秆覆盖对荠菜生长及镉积累的影响     

王恒  石军  林立金  杨代宇  黄科文  张潇 《水土保持通报》2016,36(1):184-187

[目的]研究秸秆覆盖对富集植物重金属积累的影响,筛选能促进荠菜生长和镉积累的镉耐性植物.[方法]通过盆栽试验研究镉污染条件下,覆盖4种镉耐性植物(扬子毛茛、通泉草、邻近风轮菜和车前)秸秆对荠菜生长及镉积累的影响。[结果]覆盖扬子毛茛、通泉草、邻近风轮菜和车前秸秆后,荠菜的地上部分生物量、总生物量和叶片叶绿素含量值均低于未覆盖处理。覆盖扬子毛茛、通泉草、邻近风轮菜和车前秸秆的荠菜总生物量则较未覆盖分别降低了34.04%,49.85%,40.27%和18.39%。只有覆盖邻近风轮菜秸秆提高了荠菜根系镉含量,而覆盖扬子毛茛和通泉草秸秆则提高了荠菜地上部分镉含量(较未覆盖分别提高了18.61%和12.87%)。然而,覆盖4种耐性植物的荠菜根系、地上部分及整株镉积累量均低于未覆盖。[结论]在盆栽条件下,覆盖镉耐性植物(扬子毛茛、通泉草、邻近风轮菜和车前)秸秆不能提高荠菜对镉污染土壤的修复能力。  相似文献