Invasive mould infections of the naso-orbital region of cats: a case involving Aspergillus fumigatus and an aetiological review     

Cristina Giordano  Paola Gianella  Stefano Bo  Antonella Vercelli  Chiara Giudice  Daniele Della Santa  Anna Maria Tortorano  Claudio Peruccio  Andrea Peano 《Journal of Feline Medicine and Surgery》2010,12(9):714-723

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Perturbation of the EphA2-EphrinA1 System in Human Prostate Cancer Cells by Colonic (Poly)phenol Catabolites     

Tognolini M  Giorgio C  Hassan Mohamed I  Barocelli E  Calani L  Reynaud E  Dangles O  Borges G  Crozier A  Brighenti F  Del Rio D 《Journal of agricultural and food chemistry》2012,60(36):8877-8884

The Eph tyrosine kinase receptors and their ephrin ligands play a central role in human cancer as their deregulated expression induces tumorigenesis with aggressive phenotypes. To evaluate their potential contribution to EphA2-ephrinA1 modulation, several colonic catabolites of dietary (poly)phenolics, known to be generated in vivo, were screened using an ELISA-based binding assay. Some of the catabolites inhibited the binding in a dose-dependent manner (IC(50) values from 0.26 to 43 μM). Functional studies on prostate adenocarcinoma cells revealed that pyrogallol and protocatechuic acid specifically antagonized ephrinA1-Fc-induced EphA2 phosphorylation at concentrations that were not cytotoxic. The active concentrations of pyrogallol appear to be close to what can be reached in vivo under physiological conditions. Finally, because of the roles played by the Eph-ephrin system not only in cancer development but also in neurodegeneration and diabetes, pyrogallol and protocatechuic acid are candidates for more detailed functional studies to elucidate their role in these pathophysiological processes.  相似文献   

Simulation of peak-demand hydrographs in pressurized irrigation delivery systems using a deterministic–stochastic combined model. Part II: model applications     

Daniele Zaccaria  Nicola Lamaddalena  Christopher M. U. Neale  Gary P. Merkley 《Irrigation Science》2013,31(3):193-208

A deterministic–stochastic combined model named HydroGEN was developed, as described in a companion paper (Part I: Model development), to enable the simulation of demanded daily volumes and hourly flow rates during peak periods in pressurized irrigation delivery networks. The model was applied to a pilot large-scale irrigation system located in southern Italy for calibration and for testing its reliability in analyzing the operation of large-scale pressurized delivery systems through the simulated flow configurations. Daily input data on rainfall, temperature, solar radiation, wind speed and relative humidity were gathered from a meteorological station located within the study area, whereas information on local irrigation management practices were collected through interviews with farmers and from extension specialists. The model was tested at different management levels, from district to sector and hydrants. The model testing was supported by the use of high-resolution remote-sensing imagery acquired on a single overpass date in 2006 and then classified and recoded following a ground-truthing campaign conducted during the same year. Simulations were performed to identify the 10-day peak-demand period and to generate the hydrographs of daily volumes and of hourly flow rates. Results from the different simulations were compared with historical datasets of irrigation volumes and discharges recorded during the 2008 and 2009 seasons at the upstream end of the irrigation network under study, at a sector level during the 2007 season and at selected delivery hydrants during the 2005 season. Some discrepancies between simulated and recorded data were noted that can be related to small errors in estimating crop and soil parameters, application efficiency at field level, as well as to large variability in irrigation management practices followed by local farmers. Overall, the results from testing showed that the model is capable of forecasting with good accuracy the timing of peak-demand periods, the irrigation volumes demanded during the season, as well as the hydrographs of daily volumes and hourly flow rates withdrawn by farmers during these peak-demand periods, especially when it is applied to large multi-cropped command areas.  相似文献   

Typical and atypical manifestations of Anaplasma phagocytophilum infection in dogs     

Eberts MD  Vissotto de Paiva Diniz PP  Beall MJ  Stillman BA  Chandrashekar R  Breitschwerdt EB 《Journal of the American Animal Hospital Association》2011,47(6):e86-e94

Eighteen clinically ill dogs, naturally infected with Anaplasma phagocytophilum, were examined at a veterinary practice in Baxter, Minnesota. A clinical examination, complete blood cell count, enzyme- linked immunosorbent assay (ELISA) for A phagocytophilum, Borrelia burgdorferi, and Ehrlichia canis antibodies and Dirofilaria immitis antigen, and a polymerase chain reaction test for A phagocytophilum DNA were obtained for all dogs. Physical examination findings included fever, arthropathy, lymphadenopathy, epistaxis, acute gastritis, cervical hyperpathia, and central nervous system dysfunction. Complete blood cell count abnormalities included thrombocytopenia, morulae in neutrophils, anemia, leukopenia, eosinopenia, lymphopenia, and monocytosis. Seroreactivity to A phagocytophilum was found in 61%, B burgdorferi antibodies in 17%, and D immitis antigen in 5% of the dogs. Fever, arthropathy, neurologic dysfunction, and epistaxis are clinical syndromes that can be associated with A phagocytophilum infection. Treatment with doxycycline resulted in rapid resolution of clinical signs in all dogs.  相似文献   

Identification of candidate markers on bovine chromosome 14 (BTA14) under milk production trait quantitative trait loci in Holstein     

Marques E  Grant JR  Wang Z  Kolbehdari D  Stothard P  Plastow G  Moore SS 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2011,128(4):305-313

The objective of this study was to identify single-nucleotide polymorphisms using a bovine chromosome 14 high-density SNP panel after accounting for the effect of DGAT1. Linkage disequilibrium information and sire heterozygosity were used to select markers for linkage analysis on bovine chromosome 14 for milk production traits in 321 Holstein animals. Results show putative milk peaks at 42 and 61 cM, both at p<0.10, a fat yield peak at 42 and 63 cM, both at p<0.05; a protein yield peak at 42 (p<0.01) and 84 cM (p<0.05); fat per cent peaks at 3 (p<0.01) and 29 cM (p<0.05), and a protein per cent peak at 4 cM (p<0.05). Once quantitative trait loci positions were established, allele substitution effects for all markers were evaluated using the same statistical model. Overlaying information between quantitative trait loci (QTL) and allele effect analysis enabled the identification (p<0.01) of 20 SNPs under the milk yield QTL, 2 under both of the fat yield peaks, 8 and 9 under the protein yield peaks, 2 and 6 for the fat per cent peaks and 5 for the protein per cent peak. One SNP in particular, ss61514555:A>C, showed association with 3 of the 5 traits: milk (p=1.59E-04), fat (p=6.88E-05) and protein yields (p=5.76E-05). Overall, combining information from linkage disequilibrium, sire heterozygosity and genetic knowledge of traits enabled the characterization of additional markers with significant associations with milk production traits.  相似文献   

Analysis of the effect of the bovine adenosine triphosphate-binding cassette transporter G2 single nucleotide polymorphism Y581S on transcellular transport of veterinary drugs using new cell culture models     

Real R  González-Lobato L  Baro MF  Valbuena S  de la Fuente A  Prieto JG  Alvarez AI  Marques MM  Merino G 《Journal of animal science》2011,89(12):4325-4338

In commercial dairy production, the risk of drug residues and environmental pollutants in milk from ruminants has become an outstanding problem. One of the main determinants of active drug secretion into milk is the ATP-binding cassette transporter G2/breast cancer resistance protein (ABCG2/BCRP). It is located in several organs associated with drug absorption, metabolism, and excretion, and its expression is highly induced during lactation in the mammary gland of ruminants, mice, and humans. As a consequence, potential contamination of milk could expose suckling infants to xenotoxins. In cows, a SNP for this protein affecting quality and quantity of milk production has been described previously (Y581S). In this study, our main purpose was to determine whether this polymorphism has an effect on transcellular transport of veterinary drugs because this could alter substrate pharmacokinetics and milk residues. We stably expressed the wild-type bovine ABCG2 and the Y581S variant in Madin-Darby canine kidney epithelial cells (MDCKII) and MEF3.8 cell lines generating cell models in which the functionality of the bovine transporter could be addressed. Functional studies confirmed the greater functional activity in mitoxantrone accumulation assays for the Y581S variant with a greater relative V(MAX) value (P = 0.040) and showed for the first time that the Y581S variant presents greater transcellular transport of the model ABCG2 substrate nitrofurantoin (P = 0.024) and of 3 veterinary antibiotics, the fluoroquinolone agents enrofloxacin (P = 0.035), danofloxacin (P = 0.001), and difloxacin (P = 0.008), identified as new substrates of the bovine ABCG2. In addition, the inhibitory effect of the macrocyclic lactone ivermectin on the activity of wild-type bovine ABCG2 and the Y581S variant was also confirmed, showing a greater inhibitory potency on the wild-type protein at all the concentrations tested (5 μM, P = 0.017; 10 μM, P = 0.001; 25 μM, P = 0.008; and 50 μM, P = 0.003). Differential transport activity depending on the genotype together with the differential inhibition pattern might have clinical consequences, including changes in substrate pharmacokinetics (and subsequently pharmacodynamics) and more specifically, changes in secretion of ABCG2 substrates into milk, potentially implying important consequences to veterinary therapeutics.  相似文献   

A simple and rapid method for isolation of caliciviruses from liver of infected rabbits     

Luzia Teixeira  Raquel M. Marques  Artur P. Águas  Paula G. Ferreira 《Research in veterinary science》2011,91(1):164-166

Rabbit Haemorrhagic Disease Virus (RHDV), a member of the Caliciviridae family, is the etiologic agent of Rabbit Haemorrhagic Disease (RHD); this viral disease is highly contagious and kills more than 90% of infected adult rabbits. Research on experimental calicivirus infection uses inocula obtained from livers of rabbits dying from calicivirus infection. This implies that caliciviruses have to be purified from liver homogenates. Current methods to isolate caliciviruses from rabbit livers are time consuming. We propose here a new procedure for fast purification of rabbit caliciviruses from liver homogenates that uses centrifugation through an iodixanol gradient. This method offers in approximately 2 h a sample with a high degree of calicivirus purity, as shown by its biochemical and immunocytochemistry analysis, which is also able to kill adult rabbits from RHD within 48 h of inoculation.  相似文献   

Heavy Metal Accumulation in Plant Species Indigenous to a Contaminated Portuguese Site: Prospects for Phytoremediation     

Moreira  Helena  Marques  Ana P. G. C.  Rangel  António O. S. S.  Castro  Paula M. L. 《Water, air, and soil pollution》2011,222(1-4):377-389

Acid rock drainage (ARD) often contains ferrous iron, sulphate and high concentrations of trace elements detrimental to the environment. Future costs will be enormous if the problem is not treated today. Simple and cost-effective methods for remediation of historical mine sites are therefore desired. In this study, three mine waters were mixed with alkaline ash leachates, and the fate of trace elements from both the mine waters (Cd, Cu, Pb, Zn, Ni and Co) and the ash leachates (Cr and Mo) was studied. Addition of ash water precipitates hydrous ferric oxides (HFO) and hydrous aluminium oxides (HAO) induced trace element sorption and coprecipitation. Composition of the formed HFO/HAO mix determines efficiency of the sorption and the relative order of sorption for different trace elements. Sorption occurred much earlier (often one pH unit or more) in a system with high iron concentrations compared to systems with lower iron concentrations. Removal of cadmium and zinc was low, below pH 8, if the amount of precipitates was low. Using ash for generation of alkaline water may be a problem with regard to chromium and molybdenum. This study shows that it is possible to avoid problems with molybdenum by keeping the final pH around 7, and chromium(VI) from the ash water will be reduced into chromium(III) and precipitated as the hydroxide in the presence of iron(II). Results imply that it is possible to also use fly ashes in alkaline leach beds in order to neutralize ARD followed by precipitation and sorption of trace elements.  相似文献   

Squamous cell carcinoma of the footpad with systemic metastasis in a captive crowned solitary eagle (Buteogallus coronatus)     

Camila Maria da Silva ROCHA  Tais Meziara WILSON  Davi Emanuel Ribeiro de SOUSA  Alexandra Ariadne Bittencourt Gonalves PEREIRA  Andr Santos LEONARDO  Khesller Patrícia Olazia NAME  Mrcio Botelho de CASTRO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2020,82(11):1627

Squamous cell carcinomas (SCC) are one of the most common tumors of the tegument that can have a misdiagnosis of chronic skin wounds. An adult captive crowned solitary eagle presented an indolent wound-like ulcer on the footpad and a fatal outcome. An infiltrating tumoral mass in the foot and multiple tumoral metastatic nodules in visceral organs were detected. The neoplasm was composed of atypical squamous cells with strong positivity for cytokeratin, “keratin pearl” structures, and marked invasion of tissues confirming a diagnosis of metastatic SCC. This might be the first report of an SSC with metastasis on the footpad in a captive Chaco eagle, which is one of the endangered species of birds of prey.  相似文献   

Genetic parameters for fertility traits in Nellore bulls     

Ivan Carvalho Filho  Daniele Botelho Diniz Marques  Carolina Filardi de Campos  José Domingos Guimarães  Simone Eliza Facioni Guimarães  Paulo Sávio Lopes  Fabyano Fonseca e Silva  Renata Veroneze 《Reproduction in domestic animals》2020,55(1):38-43

Nellore is the main cattle breed used in Brazil, being the largest commercial herd in the world. Beyond the importance of male reproductive efficiency for farm profit, the use of reproductive techniques, mainly artificial insemination, turns the evaluation of male reproductive traits even more important. Estimation of genetic parameters increases the knowledge on traits variances and allows envisaging the possibility of the inclusion of new traits as selection criterion. Genetic parameters for fifteen traits that can be classified as testicular biometry or physical and morphological semen traits were estimated for a Nellore bull population ranging from 18 to 36 months. Single-trait and bi-trait animal models were used for (co)variance components estimation. The contemporary group was considered as fixed effect and age at measurement as covariable. Scrotal circumference presented heritability of 0.47 ± 0.12. This value is similar to the heritabilities found for all testicular biometry traits (0.34–0.48). Sperm progressive motility, which has a direct effect on bull fertility, presented low heritability (0.07 ± 0.08). Major and total sperm defects presented moderate to high heritabilities (0.49 ± 0.18 and 0.39 ± 0.15, respectively), indicating that great genetic gain can be obtained through selection against sperm defects. High and positive genetic correlations were observed among testicular biometry traits, which also presented favourable genetic correlations with physical and morphological traits of the semen with magnitude ranging from high to low. Scrotal circumference presented moderate to high and favourable genetic correlations with sperm progressive motility, sperm turbulence, major sperm defects and total sperm defects. Thus, the selection for scrotal circumference results in favourable correlated genetic response for semen quality. The results show that the use of scrotal circumference as reference trait for bull fertility is appropriate, since it presents high heritability and favourable genetic correlation with semen quality.  相似文献