Immunohistochemical staining of chlamydial antigen in emerald tree boas (Corallus caninus).     

Elliott Jacobson  Francesco Origgi  Darryl Heard  Carol Detrisac 《Journal of veterinary diagnostic investigation》2002,14(6):487-494

Of 120 privately owned captive-bred and wild-collected emerald tree boas (ETBs) (Corallus caninus), 97 died or were euthanatized. Eighteen snakes were necropsied, and tissues were collected from all major organs and processed for light microscopy. Histologic examination demonstrated histiocytic granulomas in the small intestine, heart, and esophageal tonsils of one ETB, small intestine of a second ETB, and in an esophageal tonsil of a third ETB. Within the center of these granulomas, small, basophilic, punctate organisms were demonstrated using hematoxylin and eosin staining. Transmission electron microscopic examination of an intestinal granuloma demonstrated developmental stages of organisms consistent with members of the family Chlamydiaceae. An immunoperoxidase staining technique and 2 different commercially available monoclonal antibodies against chlamydial lipopolysaccharide antigen was used to identify chlamydial antigen in these lesions. Liver of a puff adder (Bitis arietans) with previously reported systemic chlamydiosis served as the positive control. Both monoclonal antibodies stained antigen in these granulomas. Additionally, macrophages within aggregates of lymphoplasmacytic cells in the colon, small intestine, and esophageal tonsils of 3 other ETBs contained antigen. Although both antibodies labeled antigen in serial sections of tissue, a difference in staining intensity was noted.  相似文献   

Dermatophilus chelonae in a king cobra (Ophiophagus hannah).     

James F X Wellehan  Christine Turenne  Darryl J Heard  Carol J Detrisac  Jeffrey J O'Kelley 《Journal of zoo and wildlife medicine》2004,35(4):553-556

A mass was removed from the left flank of a 10-yr-old male king cobra (Ophiophagus hannah), and histologic examination revealed granulomatous dermatitis with intralesional gram-positive cocci and filamentous bacteria. Fourteen months later, a histologically similar subcutaneous mass was removed from a different site. One year later, a large subcutaneous mass at the first surgical site was removed, and histopathologic examination revealed multiloculated granulomas with intralesional gram-positive cocci. An organism was cultured and identified by 16S ribosomal RNA gene sequencing as Dermatophilus chelonae. After a course of antibiotic therapy, no further lesions were seen for 5 mo.  相似文献   

Paramyxovirus infection in caiman lizards (Draecena guianensis).     

E R Jacobson  F Origgi  A P Pessier  E W Lamirande  I Walker  B Whitaker  I H Stalis  R Nordhausen  J W Owens  D K Nichols  D Heard  B Homer 《Journal of veterinary diagnostic investigation》2001,13(2):143-151

Three separate epidemics occurred in caiman lizards (Dracaena guianensis) that were imported into the USA from Peru in late 1998 and early 1999. Histologic evaluation of tissues from necropsied lizards demonstrated a proliferative pneumonia. Electron microscopic examination of lung tissue revealed a virus that was consistent with members of the family Paramyxoviridae. Using a rabbit polyclonal antibody against an isolate of ophidian (snake) paramyxovirus, an immunoperoxidase staining technique demonstrated immunoreactivity within pulmonary epithelial cells of 1 lizard. Homogenates of lung, brain, liver, or kidney from affected lizards were placed in flasks containing monolayers of either terrapene heart cells or viper heart cells. Five to 10 days later, syncytial cells formed. When Vero cells were inoculated with supernatant of infected terrapene heart cells, similar syncytial cells developed. Electron microscopic evaluation of infected terrapene heart cells revealed intracytoplasmic inclusions consisting of nucleocapsid strands. Using negative-staining electron microscopy, abundant filamentous nucleocapsid material with a herringbone structure typical of the Paramyxoviridae was observed in culture medium of infected viper heart cells. Seven months following the initial epizootic, blood samples were collected from surviving group 1 lizards, and a hemagglutination inhibition assay was performed to determine presence of specific antibody against the caiman lizard isolate. Of the 17 lizards sampled, 7 had titers of < or =1:20 and 10 had titers of >1:20 and < or =1:80. This report is only the second of a paramyxovirus identified in a lizard and is the first to snow the relationship between histologic and ultrastructural findings and virus isolation.  相似文献   

Infrared Spectroscopy‐Based Metabolomic Analysis for the Detection of Preharvest Sprouting in Grain     

Matthew Burke  Darryl M. Small  Frank Antolasic  Jeff G. Hughes  Michelle J. S. Spencer  Ewan W. Blanch  Oliver A. H. Jones 《Cereal Chemistry》2016,93(5):444-449

Preharvest sprouting of grains causes significant losses to growers and buyers. The problem occurs throughout the world, and economic losses can be millions of dollars. Visual assessment of germination is ineffective because even grains showing no external signs (e.g., having a visible shoot) may still have germinated sufficiently so that it is no longer possible for high‐quality products to be made from them. Current procedures to determine whether a grain has germinated are based around measuring enzyme or starch levels. However, despite protracted efforts to develop enhanced ways to measure germination over the last 50 years, there are currently no sufficiently accurate or reliable approaches available. The present work assesses the potential for infrared spectroscopy‐based metabolomic profiling to assess the germination of barley and wheat. The results indicate that mid‐ and near‐infrared spectroscopy are able to determine if the grain has germinated and give an indication of how long the germination process has been occurring. Both techniques are already well established in the grain industry and have the potential to form the basis of a simple, fast dockside test that would allow segregation of sound and mildly sprouted grain.  相似文献   

Randomization tests for time effects and heterogeneity in capture probabilities for closed populations     

Darryl I. MacKenzie  Bryan F. J. Manly 《Journal of Agricultural, Biological & Environmental Statistics》2001,6(2):292-301

We propose the use of randomization tests to detect time effects and heterogeneity in capture probabilities of individuals in a closed-population mark-recapture study. The results of a simulation study show the randomization procedures can be implemented so that tests are robust to extraneous sources of variation, with good power to detect the desired effects. We apply the tests to two examples of real data, finding very strong evidence of heterogeneous capture probabilities in both data sets and highly significant time effects for one of the sets.  相似文献   

The influence of pasture fertilisation on animal health     

C. A. H. Heard 《African Journal of Range and Forage Science》2013,30(1):134-143

The health of animals grazing on pastures is affected by the mineral content of fodder plants. The mineral content of any pasture species may vary within very wide limits and is profoundly influenced by fertilisation. Some of the known facts about animal health in relation to the supply of mineral nutrients in pasture dry matter are presented, together with a discussion of how the uptake of minerals by animals may be influenced by the fertilization of pastures.  相似文献   

NEWS FROM IVRA MEETING IN BRAZIL     

Darryl Biery 《Veterinary radiology & ultrasound》2010,51(1):103-103

  相似文献   

Distribution of Vehicular Lead in Roadside Soils of Major Roads of Brisbane,Australia   总被引：1，自引：0，他引：1  

Al-Chalabi  Abdul Sahib  Hawker  Darryl 《Water, air, and soil pollution》2000,118(3-4):299-310

Collections were made from three sites in the urbanarea of Brisbane, Australia. At each site, soilsamples were collected from transects parallel andperpendicular to the roadway, as well as a depthprofile. Total lead was determined by refluxing thesamples with concentrated nitric acid (1 h) andorganic lead by shaking with cold ammoniacal methanol(Flameless AAS). Both chloride and bromide anionswere obtained from an aqueous extract (HPLC). Resultsshowed that vehicular emissions were the major sourceof lead in the roadside soils of this study. At siteslocated in relatively enclosed areas of higheratmospheric stability, both lead and bromide contentsdecreased markedly with increasing distance from theroadway. However, in the absence of such atmosphericstability, the distribution of both lead and bromidecontents revealed different patterns which wasattributed mainly to the effect of meteorologicalfactors such as wind direction and speed. The depthprofile of roadside soils generally showed leadaccumulation within the uppermost 5 cm, whereaselevated concentrations of bromide were observed at greater depth.  相似文献   

Bone Cysts in the Dog1     

Darryl N. Biery  Michael Goldschmidt  Wayne H. Riser  W. H. Rhodes 《Veterinary radiology & ultrasound》1976,17(6):202-212

  相似文献   

Bacteremia and septic arthritis in a West African dwarf crocodile   总被引：1，自引：0，他引：1  

D J Heard  E R Jacobson  R E Clemmons  G A Campbell 《Journal of the American Veterinary Medical Association》1988,192(10):1453-1454

An anorectic, adult West African dwarf crocodile was examined because of bilateral hind limb paresis. Clinical findings included multiple skin wounds, osteomyelitis of the distal portions of the right radius and ulna, severe anemia, and Serratia marcescens bacteremia. The crocodile died after a blood transfusion. At necropsy, hemorrhage in the subarachnoid space, suppurative polyarthritis, and gastric ulceration were found. Serratia marcescens and Morganella morganii were isolated from multiple tissues and body fluids. It was concluded that the bilateral paresis was caused by severe septic arthritis secondary to bacteremia, and that the crocodile died from spinal injury caused by the blood transfusion into the supravertebral vein.  相似文献