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11.
C. Kechavarzi Q. Dawson P. B. Leeds-Harrison J. Szatyowicz & T. Gnatowski 《Soil Use and Management》2007,23(4):359-367
The rate of oxidation of peat soils is highly seasonal and varies with temperature and soil moisture content. Large variations in soil moisture content result in wet–dry cycles that can enhance peat degradation. Water‐table management plays a crucial role in controlling and damping the effect of these environmental factors. However, maintaining high ditch water levels in fields bounded by ditches does not guarantee a high field groundwater level. The effect of installing subsurface irrigation at different spacings on water table elevation was studied in a low‐lying peat grassland. The water table elevation data were compared against values predicted with a water balance model. In addition, greenhouse experiments were carried out on undisturbed soil core samples collected from the peat grassland as well as a low‐lying peatland under intensive arable faming to measure CO2 evolution under different water regimes. The field data from the peat grassland suggest that sub‐irrigation spacing as low as 10 m is necessary during summer periods to maintain groundwater levels similar to those in the ditches. Over the same period of observation, the difference in water level between the ditches and the non‐irrigated fields is as high as 0.7 m. Modelled outputs are in good correlation with the field observations, and demonstrate that simple water balance models can provide an effective tool to study the effect of water management practices and potential changes in subsurface conditions, climate and land use on water‐table levels. The measurement of CO2 emission from undisturbed peat soil columns shows that the rate of oxidation of soil organic matter from peat soils is highly seasonal and that drainage exacerbates the rate of peat mineralization. 相似文献
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Ard G. Lengkeek Alice Muchugi Mwangi Caroline A. C. Agufa Joseph O. Ahenda Ian K. Dawson 《Agroforestry Systems》2006,67(3):293-300
It is possible that current tree domestication practices undertaken by farmers reduce the genetic base of tree resources on
farms, raising concerns regarding the productivity, sustainability and conservation value of agroforestry ecosystems. Here,
we assessed possible changes in genetic variation during domestication in the important and heavily utilised timber species,
Vitex fischeri Gürke (syn. Vitex keniensis), by comparing geographically proximate forest and farm material in central Kenya. Employing RAPD analysis, a total of 104
polymorphic markers revealed by five arbitrary primers were scored in a total of 65 individuals, 32 from forest and 33 from
farmland. Despite concerns of possible genetic erosion, forest and farm stands did not differ significantly in levels of genetic
variation, with H values of 0.278 and 0.269, respectively. However, Mantel tests did reveal greater geographically related associative genetic
structure among individuals in farm rather than forest material, with r
M values of 0.217 and 0.114, respectively. A more detailed analysis of structure suggested this could be due to local variation
in origin of some on-farm trees. Implications of data for the genetic management of V. fischeri stands during farmer-led tree domestication activities are discussed. At present, there appears little reason to reject on-farm
V. fischeri as a source of germplasm for future on-farm planting or for conservation purposes, although this situation may change and
will require monitoring. 相似文献
15.
Green tea polyphenol extract regulates the expression of genes involved in glucose uptake and insulin signaling in rats fed a high fructose diet 总被引:1,自引:0,他引:1
Cao H Hininger-Favier I Kelly MA Benaraba R Dawson HD Coves S Roussel AM Anderson RA 《Journal of agricultural and food chemistry》2007,55(15):6372-6378
Green tea has antidiabetic, antiobesity, and anti-inflammatory activities in animal models, but the molecular mechanisms of these effects have not been fully understood. Quantitative real-time polymerase chain reaction (PCR) was used to investigate the relative expression levels and the effects of green tea (1 and 2 g solid extract/kg diet) on the expression of glucose transporter family genes (Glut1/Slc2a1, Glut2/Slc2a2, Glut3/Slc2a3, and Glut4/Slc2a4) and insulin signaling pathway genes (Ins1, Ins2, Insr, Irs1, Irs2, Akt1, Grb2, Igf1, Igf2, Igf1r, Igf2r, Gsk3b, Gys1, Pik3cb, Pik3r1, Shc1, and Sos1) in liver and muscle of rats fed a high-fructose diet known to induce insulin resistance and oxidative stress. Glut2 and Glut4 were the major Glut mRNAs in rat liver and muscle, respectively. Green tea extract (1 g) increased Glut1, Glut4, Gsk3b, and Irs2 mRNA levels by 110, 160, 30, and 60% in the liver, respectively, and increased Irs1 by 80% in the muscle. Green tea extract (2 g) increased Glut4, Gsk3b, and Pik3cb mRNA levels by 90, 30, and 30% but decreased Shc1 by 60% in the liver and increased Glut2, Glut4, Shc1, and Sos1 by 80, 40, 60, and 50% in the muscle. This study shows that green tea extract at 1 or 2 g/kg diet regulates gene expression in the glucose uptake and insulin signaling pathway in rats fed a fructose-rich diet. 相似文献
16.
Effects of microbial supplements containing yeast and lactobacilli on roughage-fed ruminal microbial activities 总被引:7,自引:0,他引:7
Effects of two microbial feed supplements on microbial activities in rumen-stimulating cultures and the rumens of steers fed a fescue hay-based roughage diet were evaluated. The yeast culture supplement contained Saccharomyces cerevisiae (1.4 to 4.2 x 10(9) colony-forming units [cfu]/g), whereas the mixed microbial supplement contained yeast, lactobacilli and enterococci (1.4 to 2.7 x 10(9) cfu/g, 1.2 to 2.3 x 10(9) cfu/g, and 1.5 to 2.6 x 10(10) cfu/g, respectively). Concentrations of viable yeast cells were increased consistently in continuous cultures and rumens of steers receiving either supplement (1 g/kg of feed). However, neither supplement consistently altered the relative concentrations of volatile fatty acids or ammonia in continuous cultures and rumens of steers. The pH tended to be greater (P = .13) in continuous cultures receiving yeast culture supplement than in cultures receiving the unsupplemented diet (6.50 vs 6.36), but pH in the rumens of steers was not affected by the supplements. Concentrations of cellulolytic microorganisms in cultures and the rumens of steers receiving supplements containing only yeast were from 5 to 40 times greater than those observed in cultures or steers receiving the unsupplemented diet. Supplements that had been treated with heat (121 degrees C for 15 min) to inactive yeast cells did not alter the concentrations of cellulolytic bacteria in rumen-stimulating cultures. These results suggest that live yeast culture supplements stimulate growth of cellulolytic microorganisms in the rumen. 相似文献
17.
M. Hill K. Launis C. Bowman K. McPherson J. Dawson J. Watkins M. Koziel M. S. Wright 《Euphytica》1955,85(1-3):119-123
Summary A synthetic Bt gene encoding a truncated version of the CryIA(b) protein derived from Bacillus thuringiensis was successfully introduced into elite maize using microprojectile bombardment of immature embryos. The method used to initiate and identify transformation events is described. We describe the detailed parameters used for the Biolistics device as well as the plasmids used for the transformations. The plasmids contained the synthetic Bt gene driven by either the 35S CaMV promoter or a combination of two tissue-specific promoters, leaf and pollen, derived from maize. Specific conditions for the culture of Type I callus from immature embryos, the phosphinothricin (PPT) selection protocol, and the regeneration of plants are discussed. T0 and T1 plants were initially identified using the pH-dependent chlorophenol red test and/or the histochemical -glucuronidase (GUS) assay. PCR and Southern data confirm the presence of the 35S CaMV promoter and the synthetic Bt gene. 相似文献
18.
Hydrolysis of 2,6-dichlorobenzonitrile in soils 总被引:1,自引:0,他引:1
19.
Yu SW Wang H Poitras MF Coombs C Bowers WJ Federoff HJ Poirier GG Dawson TM Dawson VL 《Science (New York, N.Y.)》2002,297(5579):259-263
Poly(ADP-ribose) polymerase-1 (PARP-1) protects the genome by functioning in the DNA damage surveillance network. PARP-1 is also a mediator of cell death after ischemia-reperfusion injury, glutamate excitotoxicity, and various inflammatory processes. We show that PARP-1 activation is required for translocation of apoptosis-inducing factor (AIF) from the mitochondria to the nucleus and that AIF is necessary for PARP-1-dependent cell death. N-methyl-N'-nitro-N-nitrosoguanidine, H2O2, and N-methyl-d-aspartate induce AIF translocation and cell death, which is prevented by PARP inhibitors or genetic knockout of PARP-1, but is caspase independent. Microinjection of an antibody to AIF protects against PARP-1-dependent cytotoxicity. These data support a model in which PARP-1 activation signals AIF release from mitochondria, resulting in a caspase-independent pathway of programmed cell death. 相似文献
20.
Cultures of skin fiibroblasts from a patient with Fabry's disease showed an accumulation of the glycolipid, galactosyl-galactosyl-glucosyl ceramide. Such cells also showed metachromasia on staining with toluidine blue and a markedly elevated acid mucopolysaccharide content. 相似文献