PrP genotypes of rare breeds of sheep in Great Britain     

Townsend SJ  Warner R  Dawson M 《The Veterinary record》2005,156(5):131-134

In total 31,669 blood samples were collected from 1187 flocks of 27 rare breeds of sheep in the UK, and their genotype profiles at the prion protein locus were determined. The frequencies of the five alleles varied widely among the breeds and some had only two of the alleles and others had all five; the average was three. The average allele frequencies across all 27 breeds were 49.7 per cent for ARR, 4.4 per cent for AHQ, 2.7 per cent for ARH, 37.4 per cent for ARQ and 5.8 per cent for VRQ. The highest frequencies for each allele were 90.7 per cent for ARR in the Leicester Longwool, 24.7 per cent for AHQ in the Hebridean, 68.7 per cent for ARH in the Manx Loghtan, 98.7 per cent for ARQ in the North Ronaldsay and 28.4 per cent for VRQ in the Boreray. All 27 breeds had the ARR allele, 21 had AHQ, 11 had ARH, 26 had ARQ and 20 had VRQ.  相似文献   

Clinical evaluation of balanced anesthesia using infusion of midazolam–ketamine–medetomidine in combination with inhalation of sevoflurane (MKM–OS anesthesia) in horses     

T Kushiro  K Yamashita  MA Umar  Y Izumisawa  K Taguchi  WW Muir 《Veterinary anaesthesia and analgesia》2005,32(4):19-19

MKM–OS anesthesia provides general anesthesia with minimum cardiovascular depression in experimental horses. The purpose of this study was to evaluate the effect of MKM–OS anesthesia in clinical cases. Sixty‐eight horses were anesthetized with MKM–OS anesthesia for selective or emergency surgery. The horse physical status was categorized based upon the American Society of Anesthesiologists (ASA) classification scheme. Forty‐four horses were classified as ASA I or II (low‐risk; 30 soft tissue, eight ophthalmic, and six orthopedic surgeries) and 24 horses were classified as ASA III to V (high‐risk; 24 emergency colic surgeries). All horses were administered medetomidine (0.005 mg kg^–1 IV) as premedication and anesthetized with ketamine (2.5 mg kg^–1 IV) and midazolam (0.04 mg kg^–1 IV). The horses were orotracheally intubated and connected to a large animal breathing circuit that delivered oxygen‐sevoflurane and administered the midazolam (0.8 mg mL^–1)‐ketamine (40 mg mL^–1)‐medetomidine (0.05 mg mL^–1) drug combination at a rate of 0.025 mL kg^–1 hour^–1. Surgical anesthesia was maintained by controlling the dial setting of the sevoflurane vaporizer and achieved by delivering 1.6–1.8% of end‐tidal sevoflurane concentration. All horses were mechanically ventilated during anesthesia. Hypercapnia and hypoxia were not sufficiently improved in high‐risk horses (PaCO₂; low‐risk 45–53 mm Hg versus high‐risk 56–60 mm Hg, p < 0.01: PaO₂ low‐risk 248–388 mm Hg versus high‐risk 95–180 mm Hg, p < 0.01). Heart rate was significantly higher in high‐risk horses (low‐risk 37–42 bpm versus high‐risk 44–73 bpm, p < 0.01). Dobutamine infusion was required in five low‐risk (11%) and 17 high‐risk horses (68%) to maintain mean arterial blood pressure >70 mm Hg. Eleven high‐risk horses died during the perioperative period (three euthanized during surgery, two died during recovery, six died after recovery). The quality of recovery was good in low‐risk horses and good to satisfactory in high‐risk horses. MKM–OS anesthesia provided excellent surgical anesthesia with minimal to mild cardiovascular depression in low risk‐horses and mild to moderate cardiovascular depression in high risk‐horses. The possibility of preserve cardiovascular function could be the advantage of MKM–OS anesthesia in high‐risk horses.  相似文献   

Limited effect of recombinant porcine interleukin-12 on porcine lymphocytes due to a low level of IL-12 beta2 receptor     

Solano-Aguilar GI  Zarlenga D  Beshah E  Vengroski K  Gasbarre L  Junker D  Cochran M  Weston C  Valencia D  Chiang C  Dawson H  Urban JF  Lunney JK 《Veterinary immunology and immunopathology》2002,89(3-4):133-148

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Secondary plant metabolites as targets for genetic modification of crop plants for pest resistance     

Glenn W. Dawson  David L. Hallahan  Alan Mudd  Madhavji M. Patel  John A. Pickett  Lester J. Wadhams  Roger M. Wallsgrove 《Pest management science》1989,27(2):191-201

Three genetic manipulation strategies are described for enhancing the effectiveness of secondary metabolites of crop plants in reducing pest and disease damage. Targets for gene transfer studies are secondary metabolites of oilseed rape, lepidopteran sex pheromones and drimane anti-feedants. Initial work towards isolation of genes for the biosynthesis of drimane anti-feedants is described.  相似文献   

Long Chain Fatty Acids of Diet as Factors Influencing Reproduction in Cattle   总被引：1，自引：0，他引：1  

JEP Santos  TR Bilby  WW Thatcher  CR Staples  FT Silvestre 《Reproduction in domestic animals》2008,43(S2):23-30

Cattle are fed moderate amounts of long chain fatty acids (FA) with the objective to enhance lactation and growth; however, recent interest on lipid feeding to cows has focused on reproduction, immunity and health. Increasing the caloric density of the ration by fat feeding has generally improved measures of cow reproduction, but when milk yield and body weight losses were increased by fat supplementation, positive effects on reproduction were not always observed. Feeding fat has influenced reproduction by altering the size of the dominant follicle, hastening the interval to first postpartum ovulation in beef cows, increasing progesterone concentrations during the luteal phase of the oestrous cycle, modulating uterine prostaglandin (PG) synthesis, and improving oocyte and embryo quality and developmental competence. Some of these effects were altered by the type of FA fed. The polyunsaturated FA of the n-6 and n-3 families seem to have the most remarkable effects on reproductive responses of cattle, but it is not completely clear whether these effects are mediated only by them or by other potential intermediates produced during rumen biohydrogenation. Generally, feeding fat sources rich in n-6 FA during late gestation and early lactation enhanced follicle growth, uterine PG secretion, embryo quality and pregnancy in cows. Similarly, feeding n-3 FA during lactation suppressed uterine PG release, and improved embryo quality and maintenance of pregnancy. Future research ought to focus on methods to improve the delivery of specific FA and adequately powered studies should be designed to critically evaluate their effects on establishment and maintenance of pregnancy in cattle.  相似文献   

Dog-human and dog-dog interactions of 260 dog-owning households in a community in Cheshire     

Westgarth C  Pinchbeck GL  Bradshaw JW  Dawson S  Gaskell RM  Christley RM 《The Veterinary record》2008,162(14):436-442

This study investigated the nature and frequency of the contacts that occur between dogs, and between dogs and people, by means of a questionnaire survey of 260 dog-owning households in a community in Cheshire, uk. The contacts were highly variable and were affected by the size, sex and age of the dog, individual dog behaviours, human behaviours and human preferences in the management of the dog. A number of situations were identified that may be important in relation to zoonoses, including sleeping areas, playing behaviours, greeting behaviours, food sources, walking, disposal of faeces, veterinary preventive treatment and general hygiene.  相似文献   

Monitoring derelict urban land: A review of problems and potentials of remote sensing techniques     

P. T. Kivell  A. J. Parsons  B. R. P. Dawson 《Land Degradation \u0026amp; Development》1989,1(1):5-21

The accelerating rate of change in urban areas calls for more rapid and cost-effective surveys of derelict land. A review of the use of remote sensing¹ for this task reveals that, although aerial photography has been used for urban analysis since the 1940s, it has not been widely adopted for surveys of derelict land, despite demonstrations of its utility. Other forms of remote sensing are similarly shown to have been little-used and to have had patchy success. The hitherto restricted utility of remote sensing is found to be due to a combination of limitations of the forms of imagery available and the methods of analysis employed. Recent changes in available remote sensing imagery, notably the higher resolution now provided by the SPOT satellite, coupled with advances in digital image-processing techniques point to improved opportunities for using remote sensing to monitor derelict land. As a demonstration of these developments, a texture image based on scene variance is derived from a SPOT image of part of north Staffordshire. All derelict and neglected land parcels larger than 1.25 ha are delimited on this image. Remote sensing techniques are not yet ready to supplant detailed ground surveys but they have a growing role to play in monitoring various aspects of land dereliction.  相似文献   

赛乐硒对发情奶牛外周血生殖激素含量和繁殖性能的影响     

刘强  黄应祥  Karl Dawson  苗朝华  高登宏  王聪  董升 《家畜生态学报》2007,28(5):52-56

选用35头处于干奶期40d、泌乳期相近和上胎平均日产奶量20kg的荷斯坦奶牛,采用完全随机区组设计分为7组,即:对照组,基础日粮(不加硒);试验组1、2、3添加亚硒酸钠,分别在基础日粮中加硒7.5mg/d、15mg/d和22.5mg/d;试验组4、5、6添加赛乐硒,分别在基础日粮中加硒7.5mg/d、15mg/d和22.5mg/d,研究赛乐硒和无机硒对奶牛发情周期生殖激素分泌和繁殖性能的影响.结果表明:赛乐硒较无机硒显著提高了发情周期促性腺激素释放激素、促卵泡素、促黄体素、雌二醇和孕酮含量,显著提高了受配率和受胎率,缩短了胎衣排出周期,促进母牛正常发情.适宜补硒量为15 mg/d.  相似文献   

Gas-liquid chromatographics determination of Bayer 73 in fish, aquatic invertebrates, mud, and water     

C W Luhning  P D Harman  J B Sills  V K Dawson  J L Allen 《Journal of the Association of Official Analytical Chemists》1979,62(5):1141-1145

A gas-liquid chromatographic (GLC) method is described for determining residues of Bayer 73 (2-aminoethanol salt of 2',5-dichloro-4'-nitrosalicylanilide) in fish muscle, aquatic invertebrates, mud, and water by analyzing for 2-chloro-4-nitroaniline (CNA), a hydrolysis product of Bayer 73. Bayer 73 residues are extracted from fish muscle tissue, invertebrates, and mud with acetone-formic acid (98+2), and partitioned from water samples with chloroform. After sample cleanup by solvent and acid-base partitioning, the concentrated extract is hydrolyzed with 2N NaOH and H2O2 for 10 min at 95 degrees C. The CNA is then partitioned into hexane-ethyl ether (7+3) and determined by electron capture GLC. Average recoveries were 88% for fish, 82% for invertebrates, 82% for mud, and 98% for water at 3 or more fortification levels.  相似文献   

Antigenic and morphological differentiation of placental and intestinal isolates of Chlamydia psittaci of ovine origin.     

P C Griffiths  H L Philips  M Dawson  M J Clarkson 《Veterinary microbiology》1992,30(2-3):165-177

Ewe placental and lamb intestinal isolates of Chlamydia psittaci recovered from flocks affected with ovine enzootic abortion were examined by inclusion morphology, indirect immunofluorescence (IIF) and immunoblot analysis. Chlamydiae recovered from the faeces of sheep from two flocks free of clinical disease were also examined. In cell culture ovine abortion (OA) and intestinal isolates were distinguishable by inclusion development and morphology. Similarly, in two-way IIF tests with one week mouse antisera isolates fell into two distinct groups: abortion or intestinal. Immunoblotting with convalescent sheep abortion antiserum identified 30 out of at least 40 silver staining polypeptides as antigenic both in OA and intestinal isolates. The serum produced a similar reaction pattern to the resolved proteins of each OA isolate, indicating a higher degree of antigenic conservation among these isolates. Considerable cross reactivity between the OA and intestinal isolates was identified, but the serum also showed apparent molecular weight differences between antigens of the two types in the 87-116 kDa, 38-44 kDa and 26-28 kDa regions. Furthermore, the immunoblotting analysis revealed heterogeneity among the intestinal isolates, particularly in antigens between 87-116 kDa and 38-44 kDa.  相似文献