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81.
A semiquantitative chemiluminescent Western blot analysis system was developed and validated to evaluate antigen-specific IgG subclass responses to electrophoretically separated proteins of Dermatophagoides farinae in healthy and atopic dogs. Both groups mounted similar D. farinae-specific IgG1 and IgG4 responses to multiple antigens, but IgG2 and IgG3 responses were difficult to detect. The most commonly recognized bands in both groups were 18 and 98 kDa antigens for IgG1 and 18, 45, 66, 98, 130 and 180 kDa for IgG4. The number of bands recognized per dog did not differ significantly, but significantly more atopic dogs had an IgG1 response to a 180 kDa protein. The overall D. farinae-specific IgG1 and IgG4 responses were slightly higher, but not significantly different, in the healthy group. The results suggest that some antigens produced by D. farinae can induce different subclass responses. However, as most of these responses are seen in both healthy and atopic dogs, they are likely to merely represent recognition of foreign proteins presented to the immune system, rather than involvement in the pathogenesis of atopic dermatitis. The role of the 180 kDa antigen warrants further study. 相似文献
82.
Floto RA MacAry PA Boname JM Mien TS Kampmann B Hair JR Huey OS Houben EN Pieters J Day C Oehlmann W Singh M Smith KG Lehner PJ 《Science (New York, N.Y.)》2006,314(5798):454-458
An effective host immune response to mycobacterial infection must control pathogen dissemination without inducing immunopathology. Constitutive overexpression of mycobacterial heat shock protein (myHsp70) is associated with impaired bacterial persistence, but the immune-mediated mechanisms are unknown. We found that myHsp70, in addition to enhancing antigen delivery to human dendritic cells, signaled through the CCR5 chemokine receptor, promoting dendritic cell aggregation, immune synapse formation between dendritic cells and T cells, and the generation of effector immune responses. Thus, CCR5 acts as a pattern-recognition receptor for myHsp70, which may have implications for both the pathophysiology of tuberculosis and the use of myHsps in tumor-directed immunotherapy. 相似文献
83.
Adamama-Moraitou KK Pardali D Day MJ Prassinos NN Kritsepi-Konstantinou M Patsikas MN Rallis TS 《Veterinary journal (London, England : 1997)》2012,191(2):261-266
Canine bronchomalacia (BM) is characterized by weakness leading to collapse of the bronchial wall. A prospective study of 18 affected dogs (age range: 1-15 years) was undertaken to characterize the clinicopathological and histological features of BM. Poodles and Yorkshire terriers were commonly affected. Half of the dogs were overweight or obese. The clinical presentation was a mild, wheezing, chronic cough and pulmonary crackles were heard in 28% of the dogs. Compatible radiographic changes were present in 61% of the dogs. Using bronchoscopy, both lungs were affected in half of the animals, whereas in the others the disease appeared to affect predominantly the left lung. Analysis of bronchoalveolar lavage fluid and biopsies of bronchial mucosa revealed pure or mixed neutrophilic inflammation. Underlying infectious bronchitis was considered possible in 56% of the dogs. It was concluded that canine BM may present as an isolated clinical entity associated with infection and/or inflammation. 相似文献
84.
Sodium chloride (NaCl) is an essential ingredient to control the functional properties of wheat dough and bread quality. This study investigated the effect of NaCl at 0, 1 and 2%, (w/w, flour base) on the gluten network formation during dough development, the dough rheology, and the baking characteristics of two commercial flours containing different levels of protein (9.0 and 13.5%) and with different glutenin-to-gliadin ratios. Examination of the dough structure by confocal microscopy at different stages of mixing show that the gluten network formation was delayed and the formation of elongated fibril protein structure at the end of dough development when NaCl was used. The fibril structure of protein influenced the dough strength, as determined by strain hardening coefficient and hardening index obtained from the large deformation extension measurements. NaCl had a greater effect on enhancing the strength of dough prepared from the low protein flour compared to those from the high protein flour. The effect of NaCl on loaf volume and crumb structure of bread followed a similar trend. These results indicate that the effect of NaCl on dough strength and bread quality may be partially compensated by choosing flour with an appropriate amount and quality of gluten protein. 相似文献
85.
86.
M. N. Saridomichelakis M. J. Day K. N. Apostolidis V. Tsioli L. V. Athanasiou A. F. Koutinas 《The Journal of small animal practice》2013,54(2):108-111
A seven‐year‐old, entire male, American Staffordshire bull terrier was diagnosed with chronic solar dermatitis and basal cell carcinoma, based on physical examination, cutaneous cytology and histopathology. Immunohistochemistry revealed that the tumour cells did not express p53. To the authors’ knowledge this is the first reported case of canine basal cell carcinoma developing as a complication of chronic solar dermatitis. 相似文献
87.
A.M. Day 《New Zealand veterinary journal》2013,61(11):300-305
An AB programme restricted to 14 days through the use of mated, 135 were treated after selection on pre-mating oestrus dates or rectal examination. Treatment was administered on the 6th day of mating. Untreated cows were inseminated, on detection of oestrus, throughout the 14 days. Nine of 135 treated cows were inseminated within 48 hours, 50 at 72 hours only, 75 at both 72 and 96 hours and one at 96 hours after treatment. All cows in season were inseminated, irrespective of recent insemination. Prolonged and split heats, and short cycles (less than 14 days), occurred in treated and untreated cows. AB conceptions confirmed in those cows retained in the herd were 623% of untreated cows (66.9% of those which were inseminated) and 69.1% of treated. The conceptions achieved in this abbreviated AB programme are equal to those normally achieved after 7 weeks of AB. Mating management is discussed; the release of labour from AB involvement 4 weeks earlier than usual is a major advantage of this technique. 相似文献
88.
89.
The reproductive tract of each of 122 cows was palpated transrectally by a veterinarian on the day before they were slaughtered, to obtain estimates of ovarian size and to identify palpable ovarian structures. Reproductive tracts were then retrieved post mortem. Ovarian volumes were recorded and compared with in vivo size scores (coded 1–5; 1 being the smallest). Ovarian volumes averaged 4.0 ml (score l), 7.0 ml (2), 10.0 ml (3), 12.9 ml (4) and 15.9 ml (5). There was a linear increase in ovarian volume of 3.0 ± 0.1 ml per score unit (r2 = 0.792). The curvilinear regression included a significant quadratic term (p<0.05), but only accounted proportionally for an additional 0.003 of the sums of squares. Ovarian size score was about proportional to volume and could be used as a non-surgical technique to measure ovarian volume in live cattle. 相似文献
90.
Justice A. Tambo Roger K. Day Julien Lamontagne-Godwin Silvia Silvestri Patrick K. Beseh Birgitta Oppong-Mensah 《国际虫害防治杂志》2020,66(4):298-310
AbstractSince its emergence in Africa in 2016, fall armyworm (FAW) has spread rapidly and poses a severe threat to the food security and livelihood of millions of smallholder farmers in the continent. Using survey data from Ghana and Zambia, we examined FAW prevention and control methods implemented by farm households and their impacts on maize output and household consumption of self-produced maize. The main control methods used included pesticide application and handpicking of larvae, while access to information on FAW was a key driver behind the implementation of the control methods. Results from an endogenous switching regression showed that the implementation of a FAW management strategy significantly enhanced maize yield and households’ own maize consumption. When disentangling the impacts of the main control methods, we found that the combination of pesticide application and handpicking of larvae produced the highest yield gain of 125%. We concluded that the current interventions put in place by farmers to tackle FAW infestations are providing positive outcomes, but successful management of the pest will require more actions, including raising awareness to enhance the adoption of control interventions and exploring other control options. 相似文献