排序方式： 共有37条查询结果，搜索用时 31 毫秒
21.
ST Rolim Filho HFL Ribeiro GMF de Camargo DF Cardoso RR Aspilcueta‐Borquis H Tonhati KB Nunes WG Vale EM Barbosa KC de Sousa 《Reproduction in domestic animals》2013,48(5):705-709
The osteopontin gene may influence the fertility of water buffaloes because it is a protein present in sperm. The aim of this work was to identify polymorphisms in this gene and associate them with fertility parameters of animals kept under extensive grazing. A total of 306 male buffaloes older than 18 months, from two farms, one in the state of Amapá and the other in the state of Pará, Brazil were used in the study. Seven SNPs were identified in the regions studied. The polymorphisms were in gene positions 1478, 1513 and 1611 in the region 5′upstrem and positions 6690, 6737, 6925 and 6952 in the region amplified in intron 5. The SNPs were associated with the traits, namely scrotal circumference, scrotal volume, sperm motility, sperm concentration and sperm pathology. There were significant SNPs (p < 0.05) for all the traits. The SNP 6690 was significant for scrotal circumference, sperm concentration, sperm motility and sperm pathology and the SNP 6737 for scrotal volume. The genotype AA of SNP 6690 presented the highest averages for scrotal circumference, sperm concentration and motility and the lowest total number of sperm pathologies. For the scrotal volume trait, the animals with the largest volume were correlated with the presence of the genotype GG of SNP 6737. These results indicate a significance of the osteopontin gene as it seems to exert a substantial influence on the semen production traits of male buffaloes. 相似文献
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Reinero CR Decile KC Byerly JR Berghaus RD Walby WE Berghaus LJ Hyde DM Schelegle ES Gershwin LJ 《American journal of veterinary research》2005,66(7):1121-1127
OBJECTIVE: To compare the effects of an orally administered corticosteroid (prednisone), an inhaled corticosteroid (flunisolide), a leukotriene-receptor antagonist (zafirlukast), an antiserotonergic drug (cyproheptadine), and a control substance on the asthmatic phenotype in cats with experimentally induced asthma. ANIMALS: 6 cats with asthma experimentally induced by the use of Bermuda grass allergen (BGA). PROCEDURES: A randomized, crossover design was used to assess changes in the percentage of eosinophils in bronchoalveolar lavage fluid (BALF); airway hyperresponsiveness; blood lymphocyte phenotype determined by use of flow cytometry; and serum and BALF content of BGA-specific IgE, IgG, and IgA determined by use of ELISAs. RESULTS: Mean +/- SE eosinophil percentages in BALF when cats were administered prednisone (5.0 +/- 2.3%) and flunisolide (2.5 +/- 1.7%) were significantly lower than for the control treatment (33.7 +/- 11.1%). We did not detect significant differences in airway hyperresponsiveness or lymphocyte surface markers among treatments. Content of BGA-specific IgE in serum was significantly lower when cats were treated with prednisone (25.5 +/- 5.4%), compared with values for the control treatment (63.6 +/- 12.9%); no other significant differences were observed in content of BGA-specific immunoglobulins among treatments. CONCLUSIONS AND CLINICAL RELEVANCE: Orally administered and inhaled corticosteroids decreased eosinophilic inflammation in airways of cats with experimentally induced asthma. Only oral administration of prednisone decreased the content of BGA-specific IgE in serum; no other significant local or systemic immunologic effects were detected among treatments. Inhaled corticosteroids can be considered as an alternate method for decreasing airway inflammation in cats with asthma. 相似文献
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Background
The relative importance of chance and determinism in structuring ecological communities has been debated for nearly a century. Evidence for determinism or assembly rules is often evaluated with null models that randomize the occurrence of species in particular locales. However, analyses of the presence or absence of species ignores the potential influence of species abundances, which have long been considered of major importance on community structure. Here, we test for community assembly rules in ant communities on small islands of the Tokelau archipelago using both presence-absence and abundance data. We conducted three sets of analyses on two spatial scales using three years of sampling data from 39 plots on 11 islands. 相似文献24.
Y.K. Eugenia Kwok Krishna Bahadur KC Jennifer J. Silver Evan Fraser 《Asia Pacific viewpoint》2020,61(1):54-70
The Tonle Sap Lake of Cambodia is one of the most productive ecosystems in the world, supporting millions of small‐scale fisher livelihoods. Women's contributions in these fisheries are often overlooked due to socio‐cultural expectations of roles and responsibilities. This is a crucial omission since climate and anthropogenic influences increasingly threaten lake inhabitants. Addressing these challenges requires the full participation of both men and women who use the lake, thus it is necessary to first understand the social dynamics of these communities. We investigated whether there were differences between men's and women's perceptions of (i) fishing and non‐fishing practices; (ii) power, access and control over fishing resources; and (iii) perceptions towards conservation and conservation areas in Pursat, Cambodia. We interviewed fishers and key informants, and found differences in perceptions of fishing and non‐fishing practices between fishermen and fisherwomen. Men more openly acknowledged unequal power dynamics, access to and control over fishing resources when compared with women. We found contrasting ideas of community fisheries and conservation between men and women, and health and safety challenges they faced in conservation areas. Findings suggest that community perspectives and unequal power relations established specific roles for women that limited their active participation in fisheries management. 相似文献
25.
D. R. Panthee R. B. KC H. N. Regmi P. P. Subedi S. Bhattarai J. Dhakal 《Genetic Resources and Crop Evolution》2006,53(1):205-212
A total of 179 garlic (Allium sativum L.) accessions were collected from various parts of Nepal in 2000. Each accession was planted in each of a plot of 2.25 m2 at the Institute of Agriculture and Animal Science (IAAS) Horticulture farm (225 m asl) and at the Agriculture Research Station,
Dailekh (1400 m asl) of Nepal Agriculture Research Council (NARC) in the first week of November 2000. All accessions were
characterized for leaf erectness, leaf color, leaf wax, leaf cross section, bulb regularity, bulb skin color, bulb outer scales
number, days to emergence, bulbing period, number of green leaves at 135 days after planting, days to maturity, plant height,
bulb weight, bulb diameter, number of cloves per bulb, clove diameter and bulb yield. Data were analyzed by using principal
component and cluster analysis procedures to reveal three major clusters. Four principal components were identified explaining
more than 86% of total variation. Major characters included in the principal components were bulb weight, diameter, yield,
number of cloves per bulb, maturity, plant height, number of green leaves at 135 days after planting, and bulbing period.
The level of variation found in the collection showed the great potentiality of improving agronomic characters in garlic. 相似文献
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JM MILLAN A. JANMAAT KC RICHARDSON LK CHAMBERS KR FOMIATTI 《Australian veterinary journal》1997,75(11):814-817
Objective To determine reference ranges for healthy yearling farmed saltwater crocodiles by performing routine biochemical and haematological laboratory tests on blood samples.
Design A clinico-pathological study.
Procedure Blood samples were collected from 120 healthy yearlings from four Northern Territory crocodile farms and body weight and length were measured. All animals had been fasted for 2 days before sample collection. Routine biochemical analytes were determined on 120 samples and haematological values determined on 30 samples (from one farm).
Results Reference ranges for biochemical and haemato-logical values were determined for farmed yearling saltwater crocodiles in the Northern Territory.
Conclusion The results were comparable with published reference ranges for other crocodilian species. Other published results of haematological values from saltwater crocodiles were from very young (6-week-old) hatchlings and older (2- to 4-year-old) crocodiles. Differences in values were presumed to be caused by age-related factors. 相似文献
Design A clinico-pathological study.
Procedure Blood samples were collected from 120 healthy yearlings from four Northern Territory crocodile farms and body weight and length were measured. All animals had been fasted for 2 days before sample collection. Routine biochemical analytes were determined on 120 samples and haematological values determined on 30 samples (from one farm).
Results Reference ranges for biochemical and haemato-logical values were determined for farmed yearling saltwater crocodiles in the Northern Territory.
Conclusion The results were comparable with published reference ranges for other crocodilian species. Other published results of haematological values from saltwater crocodiles were from very young (6-week-old) hatchlings and older (2- to 4-year-old) crocodiles. Differences in values were presumed to be caused by age-related factors. 相似文献
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Norris CR Byerly JR Decile KC Berghaus RD Walby WF Schelegle ES Hyde DM Gershwin LJ 《Veterinary immunology and immunopathology》2003,96(3-4):119-127
Allergic asthma, a Th2 cell driven response to inhaled allergens, has classically been thought of as predominantly mediated by IgE antibodies. To investigate the role of other immunoglobulin classes (e.g., IgG and IgA) in the immunopathogenesis of allergic asthma, levels of these allergen-specific immunoglobulins were measured in serum and mucosal fluids. Bermuda grass allergen (BGA)-specific IgG and IgA ELISAs in serum and bronchoalveolar lavage fluid (BALF) were developed and optimized in an experimental model of BGA-induced feline asthma. Levels of BGA-specific IgG and IgA significantly increased over time in serum and BALF after allergen sensitization. Additionally, these elevated levels of BGA-specific IgG and IgA were seen in conjunction with the development of an asthmatic phenotype indicated by positive intradermal skin tests, enhanced airways hyperreactivity, and increased eosinophil percentages in the BALF. 相似文献
30.
Norris CR Decile KC Berghaus LJ Berghaus RD Walby WF Schelegle ES Hyde DM Gershwin LJ 《American journal of veterinary research》2003,64(11):1449-1453
OBJECTIVE: To evaluate changes in cysteinyl leukotriene (LT) concentrations in urine and bronchoalveolar lavage fluid (BALF) in cats with experimentally induced asthma. ANIMALS: 19 cats with experimentally induced asthma and 5 control cats. PROCEDURE: Cats were sensitized to Bermuda grass or house dust mite allergen, and phenotypic features of asthma were confirmed with intradermal skin testing, evaluation of BALF eosinophil percentages, and pulmonary function testing. A competitive ELISA kit for LTC4, LTD4, and LTE4 was used for quantitative analysis of LTs. Urinary creatinine concentrations and BALF total protein (TP) concentrations were measured, and urinary LT-to-creatinine ratios and BALF LT-to-TP ratios were calculated. RESULTS: Mean urinary LT-to-creatinine ratios did not differ significantly between control cats and allergen-sensitized cats before or after sensitization and challenge exposure with saline (0.9% NaCl) solution or allergen, respectively. In BALF the mean LT-to-TP ratio of control cats did not differ significantly before or after sensitization and challenge exposure with saline. Asthmatic cats had BALF LT-to-TP ratios that were significantly lower than control cats at all time points, whereas ratios for asthmatic cats did not differ significantly among the various time points. CONCLUSIONS AND CLINICAL RELEVANCE: Although LTs were readily detectable in urine, no significant increases in urinary LT concentrations were detected after challenge in allergen-sensitized cats. Spot testing of urinary LT concentrations appears to have no clinical benefit for use in monitoring the inflammatory asthmatic state in cats. The possibility that cysteinyl LTs bind effectively to their target receptors in BALF and, thus, decrease free LT concentrations deserves further study. 相似文献