New Insights on the Terpenome of the Red Seaweed Laurencia dendroidea (Florideophyceae,Rhodophyta)     

Louisi Souza de Oliveira  Diogo Antonio Tschoeke  Aline Santos de Oliveira  Lilian Jorge Hill  Wladimir Costa Paradas  Leonardo Tavares Salgado  Cristiane Carneiro Thompson  Renato Crespo Pereira  Fabiano L. Thompson 《Marine drugs》2015,13(2):879-902

The red seaweeds belonging to the genus Laurencia are well known as halogenated secondary metabolites producers, mainly terpenoids and acetogennins. Several of these chemicals exhibit important ecological roles and biotechnological applications. However, knowledge regarding the genes involved in the biosynthesis of these compounds is still very limited. We detected 20 different genes involved in the biosynthesis of terpenoid precursors, and 21 different genes coding for terpene synthases that are responsible for the chemical modifications of the terpenoid precursors, resulting in a high diversity of carbon chemical skeletons. In addition, we demonstrate through molecular and cytochemical approaches the occurrence of the mevalonate pathway involved in the biosynthesis of terpenes in L. dendroidea. This is the first report on terpene synthase genes in seaweeds, enabling further studies on possible heterologous biosynthesis of terpenes from L. dendroidea exhibiting ecological or biotechnological interest.  相似文献   

Bipolaris oryzae seed borne inoculum and brown spot epidemics in the subtropical lowland rice-growing region of Brazil     

André A. Schwanck  Priscila R. Meneses  Cândida R. J. Farias  Gustavo R. D. Funck  Aline H. N. Maia  Emerson M. Del Ponte 《European journal of plant pathology / European Foundation for Plant Pathology》2015,142(4):875-885

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Nephroblastoma in a Koi (Cyprinus carpio)     

Nadia Stegeman  J. Jill Heatley  Aline Rodrigues  Roy Pool 《Journal of Exotic Pet Medicine》2010,19(4):298-303

A cachexic 2-year-old koi (Cyprinus carpio) presented with progressive distension of the coelom (1-month period) and anorexia of 2 days' duration. The fish had been maintaining a normal swimming position, although it was often observed low in the water column. Water quality was within normal limits. A skin scrape, fin clip, and gill clip were unremarkable. The respiration rate (opercular movement) was elevated at 100 breaths per minute. Blood chemistry values demonstrated a hypoalbuminemia (1.3 g/dL), elevated uric acid (1.3 mg/dL), elevated creatine kinase (>14,000 U/L), and hyperkalemia (3.9 mmol/L). Ultrasound revealed a fluid-filled coelom and a soft tissue mass containing large vessels filling most of the coelomic cavity. Fine-needle aspirates of the mass were nondiagnostic, though a poorly exfoliating mesenchymal tumor was suspected. The mass was surgically excised. Pathological examination of the mass revealed it to be a poorly demarcated and unencapsulated neoplasm forming 3 histological patterns in which a spindle cell stromal component predominated over an intermediate-sized blastema and much smaller tubular patterns. These features were most consistent with a nephroblastoma. The koi survived 5 days postoperatively but remained low in the water column and was found dead on day 6. A full necropsy of this patient was not performed.  相似文献   

Potential of terracing to reduce glyphosate and AMPA surface runoff on Latosol     

Exterkoetter  Ronan  Rozane  Danilo Eduardo  da Silva  Walquiria Chaves  Toci  Aline Theodoro  Cordeiro  Gilcelia Aparecida  Benassi  Simone Frederigi  Boroski  Marcela 《Journal of Soils and Sediments》2019,19(5):2240-2250

Journal of Soils and Sediments - Glyphosate is the world’s most used herbicide and monitoring glyphosate in the environment is a relevant topic. The present study aims to develop a...  相似文献   

Diphenyl diselenide‐loaded nanocapsules in silver catfish feed enhance growth,improve muscle antioxidant/oxidant status and increase selenium deposition: Advantages of nanotechnology for fish health     

Matheus D. Baldissera  Carine F. Souza  Keiti O. Alessio  Katiane W. Krawczak  Lorenzo B. Abbad  Aleksandro S. da Silva  Csar Bizzi  Aline F. Ourique  Carla C. Zeppenfeld  Bernardo Baldisserotto  Mauro A. Cunha 《Aquaculture Research》2020,51(10):4196-4205

The aim of this study was to determine whether feed supplementation with nanoencapsulated Ph₂Se₂ would improve the growth performance of silver catfish and would maintain or improve the muscle antioxidant system. Final mean weight (p = .031), weight gain (p = .014) and specific growth rates (p = .045) were significantly higher in supplemented fish than in the control group. Muscle reactive oxygen species (p = .0001) levels were significantly lower in supplemented fish than in the control group. Also, muscle hydrogen peroxide (p = .0001) and lipid peroxidation (p = .0001) levels were significantly lower in supplemented fish than in the control group. Muscle antioxidant capacity against peroxyl radical (p = .00001) levels were significantly higher in fish supplemented with both Ph₂Se₂ forms (free or nanocapsules) than in the control group, while muscle superoxide dismutase (p = .001) and catalase (p = .0001) activities were significantly higher in supplemented fish than in the control group. Finally, selenium (Se) deposition (p = .00001) was significantly higher in the muscle of fish supplemented with free and nanoencapsulated forms compared with the control; and deposition levels were significantly higher in fish supplemented with Ph₂Se₂‐loaded in nanocapsules compared with the free form. Based on this evidence, Ph₂Se₂‐loaded in nanocapsules improves silver catfish performance and antioxidant/oxidant status. The greater content of Se (1.22 µg/g muscle) in the muscle of fish fed with dietary Ph₂Se₂‐loaded nanocapsules indicates that it may be considered a nutraceutical for fish.  相似文献   

Finishing plant diet supplemented with microalgae meal increases the docosahexaenoic acid content in Colossoma macropomum flesh     

Carlos Andre Amaringo Cortegano  Aline Marculino de Alcntara  Allana Feitoza da Silva  Cludia Maiza Fernandes Epifnio  Sunique Po Cordeiro Bentes  Vanessa Jorge dos Santos  Jesuí Vergílio Visentainer  Ligia Uribe Gonalves 《Aquaculture Research》2019,50(4):1291-1299

The need for a sustainable aquaculture is increasing the use of plant ingredients in replacement to fishmeal and fish oil in diets for tambaqui Colossoma macropomum, which is leading to not detectable levels of docosahexaenoic acid (DHA) in its flesh. We evaluated the effect of a finishing plant diet supplemented with 5% of microalgae meal from Schizochytrium sp. (MD) on tambaqui growth, on proximal composition and fatty acid content of its flesh, comparing it to a non‐supplemented diet. One hundred and sixty‐two fish (489.67 g) were distributed into six tanks (2,000 L) and fed the experimental diets for a 90‐day period. Three fish per tank were euthanized for analyses every 15 days. The MD did not affect the growth and proximal composition of fish flesh. The MD increased the DHA content (from 14.81 to 38.60 mg/g of lipids) and the n‐3:n‐6 ratio (from 0.16 to 0.51) in the flesh of fish, beginning on the 15th day and reaching the highest DHA content on the 71st day (39.81 mg/g of lipids). We recommend C. macropomum to be fed with a finishing diet supplemented with microalgae meal for 71 days before slaughter to improve the DHA content and n‐3:n‐6 ratio in the flesh.  相似文献   

Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from Dunaliella salina     

Guillaume Tanguy  Aline Legat  Olivier Gonalves  Luc Marchal  Benoît Schoefs 《Marine drugs》2021,19(11)

Biocompatible extraction emerges recently as a means to reduce costs of biotechnology processing of microalgae. In this frame, this study aimed at determining how specific culture conditions and the associated cell morphology impact the biocompatibility and the extraction yield of β-carotene from the green microalga Dunaliella salina using n-decane. The results highlight the relationship between the cell disruption yield and cell volume, the circularity and the relative abundance of naturally permeabilized cells. The disruption rate increased with both the cell volume and circularity. This was particularly obvious for volume and circularity exceeding 1500 µm³ and 0.7, respectively. The extraction of β-carotene was the most biocompatible with small (600 µm³) and circular cells (0.7) stressed in photobioreactor (30% of carotenoids recovery with 15% cell disruption). The naturally permeabilized cells were disrupted first; the remaining cells seems to follow a gradual permeabilization process: reversibility (up to 20 s) then irreversibility and cell disruption. This opens new carotenoid production schemes based on growing robust β-carotene enriched cells to ensure biocompatible extraction.  相似文献   

Evaluation of a wettable powder formulation for the nuclear polyhedrosis virus of Anticarsia gemmatalis (Lep.: Noctuidae)     

Claudia C. Medugno  Jos M. G. Ferraz  Aline de H. N. Maia  Cludio C. L. Freitas 《Pest management science》1997,51(2):153-156

The influence of different carriers on the physical and biological stability of Baculovirus anticarsia wettable powder formulations was studied. The formulations were obtained by mixing the purified polyhedra of B. anticarsia with a carrier and drying the suspension in a bench spray dryer. Bioassays with Anticarsia gemmatalis Hübner showed that activity was maintained with the amorphous silica, attapulgite and kaolinite after a year of storage. In the presence of bentonite, activity declined 50% in the same period. All formulations, except kaolinite, maintained the physical parameters required of a good wettable powder. Kaolinite formulation showed reduction in wettability and particle agglomeration in storage. © 1997 SCI  相似文献   

Effect of Mixing Conditions on the Behavior of Lipoxygenase,Peroxidase, and Catalase in Wheat Flour Doughs     

Jean-Franois Delcros  Lalatiana Rakotozafy  Aline Boussard  Sylvie Davidou  Catherine Porte  Jacques Potus  Jacques Nicolas 《Cereal Chemistry》1998,75(1):85-93

The effect of mixing has been tested on the extractable activities of lipoxygenase, peroxidase, and catalase from dough after 2, 5, and 20 min of mixing, and 30 min of rest period after 20 min of mixing. Different mixing conditions have been studied including temperature, atmosphere, speed, amount of water added to the dough, buffer solutions between pH 3.6 and 7.5 added to the dough, and different additives (linoleic acid, guaiacol, hydrogen peroxide, ascorbic acid, cysteine, yeast, and sodium chloride). In all the mixing conditions tested, the dough peroxidase activity remains equivalent to the initial flour activity, whereas losses in lipoxygenase and catalase activities largely varied according to mixing conditions. The results show that a self-destruction mechanism as well as physicochemical denaturation are responsible for these losses. Lipoxygenase losses seem mainly associated with the former mechanism, whereas catalase losses are highly increased in acidic conditions (physicochemical denaturation). Therefore, the relative impact of the three oxidoreducing enzymes may be largely modulated by mixing conditions.  相似文献   

MALDI‐imaging analyses of honeybee brains exposed to a neonicotinoid insecticide     

Aline F Catae  Anally R da Silva Menegasso  Marcel Pratavieira  Mario S Palma  Osmar Malaspina  Thaisa C Roat 《Pest management science》2019,75(3):607-615

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