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排序方式: 共有98条查询结果,搜索用时 234 毫秒
21.
Alain Regnier Clara Landrevie Marie-Claire Robin Delphine Einsweiler Jean-Yves Douet Isabelle Raymond-Letron 《Veterinary ophthalmology》2023,26(1):62-70
A 6-year-old miniature Shetland pony mare was referred for evaluation of a left corneal mass, which developed from the healing tissue of a corneal traumatic ulceration that had occurred 4 weeks previously. On gross examination, a spherical, smooth-surfaced, and pink-colored lesion of about 1 cm in diameter was protruding from the left palpebral fissure. Ophthalmic examination revealed that it was attached to the scar tissue of the cornea, and that one corpora nigra was adherent to the posterior face of corneal wounded area, without sign of uveitis. The remainder of the ophthalmic examination was unremarkable. The mass was excised, and cryotherapy was used as an adjunctive therapy. Histopathology of the resected mass was consistent with a pyogenic granuloma on the basis of radially oriented proliferating capillaries, embedded in immature granulation tissue containing an infiltrate of neutrophils, plasma cells and eosinophils. There were no histological features of malignancy. 2 months after surgery, the ventral part of the fibrotic corneal scar was slightly raised by a pink tissue, suggesting possible recurrence of the initial lesion. A second cryotherapy was performed over the leukoma area. No recurrence has been noted for a follow-up period of more than 25 months. Pyogenic granuloma is a benign proliferative fibrovascular response that typically develops after trauma or surgery. Corneal involvement is rare in humans, and to the authors' knowledge has never been documented in veterinary ophthalmology. 相似文献
22.
A prospective study was performed in 34 fasted healthy cats to describe the normal ultrasonographic anatomy of the cardia and pylorus. Measurements were obtained for the caudal esophageal wall thickness (Ew), cardia wall thickness (Cw), pyloric wall thickness (Pw), thickness of the pyloric muscularis (Mp), length of the thicker part of the proximal duodenal submucosa (Dl). Among the 34 cats, 24 were examined using a linear transducer, and 10 with a microconvex transducer. Ew and Cw could be measured in 70% of the cats when a linear transducer was used, in 100% of the cats when a microconvex probe was used, Pw and Mp could be measured in 100% of the cats whatever probe was used. The submucosa of the most proximal part of the duodenum was thicker in half of the cats in longitudinal section. The muscularis layer of the pylorus was triangular in longitudinal section and thicker than the muscularis of the proximal duodenum. The mean for Ew, Cw, Pw, Mp, and DI was 4.9 mm (SD = 1.1), 5 mm (SD = 0.6), 4.4 mm (SD = 0.6), 2.5 mm (SD = 0.5), and 4.7 mm (SD = 2.38), respectively. Three cats with abnormalities of the cardia and pylorus are also described to illustrate clinical implications. 相似文献
23.
Comparison of sandy soils suppressive or conducive to ectoparasitic nematode damage on sugarcane 总被引:1,自引:0,他引:1
ABSTRACT Two South African sandy soils, one suppressive and the other conducive to ectoparasitic nematode damage on monoculture sugarcane, were compared. Analysis of field transects indicated that the suppressive soil displayed a comparatively higher population of the weak ectoparasite Helicotylenchus dihystera, whose predominance among ectoparasitic nematodes is known to limit yield loss caused by more virulent phytonematodes. Soil type was identical at both sites (entisols), but the suppressive soil had a higher organic matter content and a lower pH, which correlated with H. dihystera population data. In contrast, microclimatic differences between the two field sites were unlikely to be responsible for the suppressive or conducive status of the soils, as shown in a greenhouse experiment. The two soils exhibited a bacterial community of the same size but with different genetic structures, as indicated by automated ribosomal intergenic spacer analysis (RISA). The number of culturable fluorescent pseudomonads was higher for the conducive soil, probably because extensive root damage caused by ectoparasitic nematodes favored proliferation of these bacteria. This study shows that apparently small differences in soil composition between fields located in the same climatic area and managed similarly can translate into contrasted nematode communities, ectoparasitic nematode damage levels, and sugarcane yields. 相似文献
24.
Delphine Ansan-Melayah Thierry Rouxel Jean Bertrandy Bruno Letarnec Edouard Mendes-Pereira Marie-Hélène Balesdent 《European journal of plant pathology / European Foundation for Plant Pathology》1997,103(9):835-841
Field experiments were conducted in Versailles, France, to assess blackleg resistance of Brassica napus cultivars Quinta and Glacier under natural infection conditions. Blackleg disease severity was assessed twice during growth of B. napus. Quinta resistance was highly expressed as only 13% to 18% of the plants exhibited leaf symptoms in December, whereas Glacier and other cultivars displayed more than 80% of infected plants. In June (harvest), 70% (first year) to 41.5% (second year) of Quinta plants were canker-free. In contrast, Glacier was as infected as the susceptible control cultivars, with more than 88% of plants displaying canker. The Leptosphaeria maculans population structure was examined in parallel. Based on soluble protein patterns, 9% of the 299 fungal isolates collected were characterized as Tox0 species, and belonged to the NA1 sub-group. All but two of Tox0 isolates were isolated from atypical dark necrotic leaf lesions, mainly occurring on Quinta. In contrast, the Tox+ isolates were recovered from typical leaf lesions. Following a cotyledon inoculation test on the differential set Westar, Quinta and Glacier, 92 to 95% of Tox+ isolates collected on susceptible cultivars were characterized as PG3 isolates, i.e. avirulent on Quinta. The remaining Tox+ isolates belong to PG4, i.e. virulent on the three cultivars. No PG2 isolate, i.e. avirulent on both Quinta and Glacier, was identified in the sampling. The present study suggests that specific resistance expressed at the cotyledon level can be efficient under field conditions where the corresponding avirulent races of the pathogen are prevalent. 相似文献
25.
Alain Chapon Morgane Boutin Delphine Rimé Laurie Delalande Anne-Yvonne Guillerm Alain Sarniguet 《European journal of plant pathology / European Foundation for Plant Pathology》2003,109(1):61-70
The efficacy of fluorescent pseudomonads as suppressors of soil-borne diseases is linked to their ability to colonise plant roots. Monitoring the dynamics of biocontrol agents in the rhizosphere should improve the irreliability. We designed a pair of Sequenced Characterised Amplified Region (SCAR) primers specific to Pseudomonas fluorescens Pf29A, based on a specific 700 bp RAPD product selected in a previous work. Primer specificity was tested with DNA samples extracted from rhizospheric soil and rhizoplane of wheat plants grown in two different non-sterile soils. We assessed the total population of Pf29A by PCR and the culturable population by counting a tetracycline-resistant Pf29A transformant producing Green Fluorescent Protein (GFP), on selective medium 5 days after inoculation of non-sterile soil. SCAR primers were specific for Pf29A in both soils. We evaluated the limit of detection to 14.2 fg of target DNA, equivalent to 242 Pf29A cells per cm of wheat root. Culturable populations of Pf29A transformant accounted for 13% and 4% of the total populations 5 days after treatment with 103 and 107 CFU of transformed Pf29A per gram of soil. The SCAR derived sequence is a good candidate to develop a strain specific and sensitive PCR-quantification of Pf29A available for population dynamic studies in fields. We confirm that only a small proportion of the total Pf29A rhizosphere population is culturable. 相似文献
26.
27.
Mitchell MA Solangi MA Clemons-Chevis CL Vanderpool D Romagnoli M Hoffland T Jowett P 《American journal of veterinary research》2008,69(7):900-903
OBJECTIVE: To determine the correlation between plasma iron concentrations and gastric pH in a population of captive Atlantic bottlenose dolphins (Tursiops truncatus). ANIMALS: 6 adult female dolphins that ranged from 16 to 30 years of age. PROCEDURES: Blood and gastric samples were collected from each dolphin to allow measurement of plasma iron concentrations and gastric pH, respectively. Samples were collected each month for 12 months. RESULTS: Within each dolphin, plasma iron concentrations and gastric pH did not differ significantly over time. There was a strong negative correlation (r = -0.85) between plasma iron concentration and gastric pH, which suggested that dolphins with a lower gastric pH had a higher plasma iron concentration. CONCLUSIONS AND CLINICAL RELEVANCE: Analysis of results reported here suggested that gastric pH may play an important role in iron absorption in dolphins. 相似文献
28.
Delphine?Allorent Serge?SavaryEmail author 《European journal of plant pathology / European Foundation for Plant Pathology》2005,113(4):329-341
In this review, available knowledge on angular leaf spot (ALS) of bean, caused by Phaeoisariopsis griseola, is analysed and synthesised. This is done through a systems-analytical approach, and successive flowcharts of the system,
in order to identify knowledge gaps and guide further research. Six connected sub-models of the ALS monocycle are used as
a framework: lesion establishment, lesion extension, defoliation, sporulation, spore liberation, and spore deposition. Each
of the sub-models enables the linking of processes to various effects of environmental (physical and host) factors. Disease-induced
defoliation is one feature of the pathosystem, leading to a depletion of infectious tissues from the canopy, which are transferred
to the ground. Consequences of defoliation may include: strong reductions of the amount of inoculum and of vacant sites in
the canopy, limited maximum disease severity, and progressive accumulation of inoculum below the canopy, which may become
important a later stage of disease epidemics. These elements are hypothesised to explain the typical behaviour of ALS epidemics
in the field, especially late onset, high apparent rate of disease increase, and low level of terminal disease severity in
the standing canopy. Epidemiological consequences of lesion expansion, sporulation, and survival of spores deposited onto
the canopy are other knowledge gaps in this pathosystem. 相似文献
29.
Delphine?Allorent Laetitia?Willocquet Aloisio?Sartorato Serge?SavaryEmail author 《European journal of plant pathology / European Foundation for Plant Pathology》2005,113(4):377-394
Daily multiplication factor (number of daughter lesions per mother lesion per day) values were experimentally measured in
four replications of a monocyclic experiment on angular leaf spot (ALS) of bean, where sources of inoculum were artificially
established within a bean canopy, on the ground (defoliated infected leaves), or both. Daily multiplication factor of lesions
in the canopy (DMFRc) was higher than that of infectious, defoliated tissues (DMFRd) in all replications. Both DMFRc and DMFRd
were strongly reduced under dry compared to rainy conditions. Under rainy conditions for spore dispersal DMFRd was about two
to three times smaller than DMFRc. Defoliated leaves may nevertheless represent a significant source of infection, depending
on the amount of infectious tissues. Mother lesions within the canopy generated more daughter lesions in the medium (or lower)
layers of the canopy than at its upper level (DMFRc higher at the medium and lower layers of a canopy), whereas DMFRd values
seemed to decrease with height in the canopy. A mechanistic simulation model that combines host growth and disease-induced
defoliation was designed to simulate the respective contributions of the two components of the dual inoculum source of a diseased
canopy (infected foliage and defoliated infectious tissues), and varying infectious periods in both sources. Simulations suggest
that higher DMFRc values have a large polycyclic effect on epidemics whereas that of DMFRd is small, and that large effects
of the infectious period of lesions in the canopy are found when DMFRc is high. Simulations using experimentally measured
DMFRc and DMFRd values indicated much stronger epidemics in rainy compared to dry conditions for spore dispersal, but disease
persistence in the latter. The implications of considering a dual source of inoculum in the course of a polycyclic process
are discussed with respect to epidemic thresholds. 相似文献
30.
Delphine Bibbal Monique Kérourédan Estelle Loukiadis Flemming Scheutz Eric Oswald Hubert Brugère 《Veterinary microbiology》2014,168(2-4):451-454
Enteroaggregative Shiga-toxin-producing Escherichia coli strains were responsible for a massive outbreak in Europe in 2011, and had been previously isolated from French patients. The objective of this study was to investigate the presence of enteroaggregative E. coli (EAEC) in slaughterhouse effluents (wastewater, slurry, sludge and effluents), and in river waters near these slaughterhouses. A total of 10,618 E. coli isolates were screened by PCR for the presence of EAEC-associated genetic markers (aggR, aap and aatA). None of these markers was detected in E. coli isolated from slaughterhouse samples. A unique enteroaggregative E. coli (EAEC) O126:H8 was detected in river water sampled upstream from slaughterhouse effluent discharge. These results confirmed that animals might not be reservoirs of EAEC, and that further studies are required to evaluate the role of the environment in the transmission of EAEC to humans. 相似文献