Beta 1-6 branching of Asn-linked oligosaccharides is directly associated with metastasis   总被引：27，自引：0，他引：27  

J W Dennis  S Laferté  C Waghorne  M L Breitman  R S Kerbel 《Science (New York, N.Y.)》1987,236(4801):582-585

Neoplastic transformation has been associated with a variety of structural changes in cell surface carbohydrates, most notably increased sialylation and beta 1-6-linked branching of complex-type asparagine (Asn)-linked oligosaccharides (that is, -GlcNAc beta 1-6Man alpha 1-6Man beta 1-). However, little is known about the relevant glycoproteins or how these transformation-related changes in oligosaccharide biosynthesis may affect the malignant phenotype. Here it is reported that a cell surface glycoprotein, gp 130, is a major target of increased beta 1-6-linked branching and that the expression of these oligosaccharide structures is directly related to the metastatic potential of the cells. Glycosylation mutants of a metastatic tumor cell line were selected that are deficient in both beta 1-6 GlcNAc transferase V activity and metastatic potential in situ. Moreover, induction of increased beta 1-6 branching in clones of a nonmetastatic murine mammary carcinoma correlated strongly with acquisition of metastatic potential. The results indicate that increased beta 1-6-linked branching of complex-type oligosaccharides on gp 130 may be an important feature of tumor progression related to increased metastatic potential.  相似文献   

Women in science. Japanese societies tackle gender issues     

Normile D 《Science (New York, N.Y.)》2002,298(5591):36

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The promise and pitfalls of clinical trials overseas     

Normile D 《Science (New York, N.Y.)》2008,322(5899):214-216

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Tsunami research. Did tsunamis influence Polynesian history?     

Normile D 《Science (New York, N.Y.)》2011,334(6061):1342

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A single IGF1 allele is a major determinant of small size in dogs   总被引：3，自引：0，他引：3  

Sutter NB  Bustamante CD  Chase K  Gray MM  Zhao K  Zhu L  Padhukasahasram B  Karlins E  Davis S  Jones PG  Quignon P  Johnson GS  Parker HG  Fretwell N  Mosher DS  Lawler DF  Satyaraj E  Nordborg M  Lark KG  Wayne RK  Ostrander EA 《Science (New York, N.Y.)》2007,316(5821):112-115

The domestic dog exhibits greater diversity in body size than any other terrestrial vertebrate. We used a strategy that exploits the breed structure of dogs to investigate the genetic basis of size. First, through a genome-wide scan, we identified a major quantitative trait locus (QTL) on chromosome 15 influencing size variation within a single breed. Second, we examined genetic variation in the 15-megabase interval surrounding the QTL in small and giant breeds and found marked evidence for a selective sweep spanning a single gene (IGF1), encoding insulin-like growth factor 1. A single IGF1 single-nucleotide polymorphism haplotype is common to all small breeds and nearly absent from giant breeds, suggesting that the same causal sequence variant is a major contributor to body size in all small dogs.  相似文献   

N-linked glycosylation in Campylobacter jejuni and its functional transfer into E. coli     

Wacker M  Linton D  Hitchen PG  Nita-Lazar M  Haslam SM  North SJ  Panico M  Morris HR  Dell A  Wren BW  Aebi M 《Science (New York, N.Y.)》2002,298(5599):1790-1793

N-linked protein glycosylation is the most abundant posttranslation modification of secretory proteins in eukaryotes. A wide range of functions are attributed to glycan structures covalently linked to asparagine residues within the asparagine-X-serine/threonine consensus sequence (Asn-Xaa-Ser/Thr). We found an N-linked glycosylation system in the bacterium Campylobacter jejuni and demonstrate that a functional N-linked glycosylation pathway could be transferred into Escherichia coli. Although the bacterial N-glycan differs structurally from its eukaryotic counterparts, the cloning of a universal N-linked glycosylation cassette in E. coli opens up the possibility of engineering permutations of recombinant glycan structures for research and industrial applications.  相似文献   

WATCHING A SNAKE FOR AN HOUR     

Dennis W 《Science (New York, N.Y.)》1892,20(515):338-339

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Homologs of small nucleolar RNAs in Archaea     

Omer AD  Lowe TM  Russell AG  Ebhardt H  Eddy SR  Dennis PP 《Science (New York, N.Y.)》2000,288(5465):517-522

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Polyhydroxybutyrate, a biodegradable thermoplastic, produced in transgenic plants     

Poirier Y  Dennis DE  Klomparens K  Somerville C 《Science (New York, N.Y.)》1992,256(5056):520-523

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Biomedical science. The lure of first-class science     

Normile D 《Science (New York, N.Y.)》2002,297(5586):1472

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