An Investigation of the Ability of the Glutaraldehyde Test to Distinguish between Acute and Chronic Inflammatory Disease in Horses     

P Brink  JC Wright  J Schumacher 《Acta veterinaria Scandinavica》2005,46(2):69-78

The Glutaraldehyde test (GT), a rapid and inexpensive test, has been utilized empirically for many years in bovine practice for diagnosing inflammatory diseases. GT is used primarily to demonstrate increased serum concentrations of fibrinogen and globulin. Glutaraldehyde binds with free amino groups in fibrinogen and immunoglobulin to create a clot in a first degree chemical reaction. The clotting time of the GT estimates the content of proteins produced in response to inflammation. The applicability of GT for diagnosing inflammation in the horse has never been investigated. The objective of this study was to determine the ability of GT to distinguish between acute and chronic inflammatory disease in horses. Thirty-seven horses with suspected inflammatory diseases were evaluated using the GT, history, complete clinical examination and routine blood analysis. GT-times, laboratory results and clinical outcome were compared statistically. Horses that were determined to be acutely affected (based on history, clinical examination and routine blood analysis) tended to have a negative GT (75%). Results of the GT did not correlate with blood fibrinogen concentration. Positive GT also predicted a fatal outcome in 69% of the clinical cases. The results of this trial indicate that GT can be a useful screening test to distinguish between acute and chronic inflammatory disease in horses.  相似文献   

Ovum Pick-up in Sheep: a Comparison between Different Aspiration Devices for Optimal Oocyte Retrieval     

C Rodríguez  L Anel  M Alvarez  E Anel  JC Boixo  CA Chamorro  P de  Paz 《Reproduction in domestic animals》2006,41(2):106-113

In vivo ovum pick-up (OPU) in sheep may be improved with a proper choice of aspiration elements (needle and tubing) and aspiration vacuum pressure. In the present study, two experiments were carried out. In Expt 1, visible follicles in ovaries of slaughtered ewes (treated separately according to their diameters: small<3 mm, medium 3-5 mm and large>5 mm) were aspirated using different combinations of the three studied factors such as aspiration flow rate (10, 20, 30, 40 and 50 ml water/min), needle gauge (18 and 20 G) and tubing inner diameter (1, 2 or 3 mm internal diameter). In Expt 2, a study with two 18 G needles of different lengths (18 G: 82 mm; 18 GL: 600 mm) was carried out, using ovaries obtained post-mortem, and performing in vivo laparoscopic follicular aspiration on ewes. We considered good quality oocytes as those with both complete compact cumulus and a homogeneous cytoplasm. Recovery rate, proportion of good quality oocytes (good quality oocytes/100 oocytes recovered) and overall efficiency (good quality oocytes/100 follicles aspirated) were noted. In Expt 1, aspiration flow rate affect remarkable proportion of good quality oocytes (69.5%, 50.5%, 44.8%, 36.5% and 28.3% for flows from 10 to 50 ml/min respectively, p<0.05). Needle gauge did not affect aspiration device efficiency. Thin and intermediate tubings were more effective (overall efficiency rates: 34.9%, 32.3% and 28.1% for 1, 2 and 3 mm respectively, p<0.05). Follicle size did not affect recovery rate, but proportion of good quality oocytes was higher for large (77.9%) and medium (64.4%) follicles (p<0.05). Finally, some combinations of the aspiration device showed greater effectiveness. In Expt 2, needle length did not influence recovery rate, but good quality oocytes rate was significantly modified both post-mortem and in vivo (good quality rate for 18 G vs 18 GL needles: 69.5% vs 47.7% and 58.1% vs 25.4%, post-mortem and in vivo respectively, p<0.05). We conclude that low-aspiration flow rates (10 and 20 ml/min) with thin or intermediate tubings (1 and 2 mm), and any short needle (18 G or 20 G) are the most adequate aspiration factors for OPU in sheep.  相似文献   

Ways to Improve the Biosecurity of Bovine Semen     

L de Ruigh  JC Bosch  MC Brus  B Landman  JS Merton 《Reproduction in domestic animals》2006,41(4):268-274

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Growth response of six weed species and spring barley (Hordeum vulgare) to increasing levels of nitrogen and phosphorus   总被引：1，自引：0，他引：1  

C ANDREASEN  A-S LITZ  & JC STREIBIG 《Weed Research》2006,46(6):503-512

A glasshouse experiment was carried out to investigate the influence of increasing levels of nitrogen and phosphorus on the growth of six common weed species growing alone or in competition with spring barley (Hordeum vulgare). Capsella bursa‐pastoris, Chenopodium album, Papaver rhoeas, Sinapis arvensis, Spergula arvensis, Viola arvensis and spring barley were grown in pots with different levels of nitrogen (0, 30, 60, 90, 120 and 150 kg N ha^?1) or phosphorus (0, 10, 20, 30, 40 and 60 kg P ha^?1). The aboveground parts of the plants were harvested after 7 weeks and the dry weight of shoots, percentage N and P content of the shoot and uptake of N and P were determined. A linear or a polynomial model was used to describe the data. Growing alone, Spergula arvensis was the only weed species that increased its dry weight at the same rate as barley. Weed species with low dry weight increase had larger increases in percentage N or P content than barley, indicating a luxury accumulation of nutrients. The uptake of N and P per pot did not differ much between weeds and barley. V. arvensis and P. rhoeas accumulated least nutrients (per cent of dry matter) and Spergula arvensis accumulated most. Weeds grew poorly in competition with barley. The percentage N and P content in barley did not change when they grew in competition with weeds.  相似文献   

A survey of xylan degradation by wood-decaying bacterial isolates     

A. M. Deschamps  J. M. Lebeault 《Forest Pathology》1980,10(5):316-319

A collection of bacterial strains isolated from decaying or living wood and bark samples were tested for xylan degradation and assimilation. This character was widely distributed among the isolates and new genera of xylan-degrading bacteria were obtained.  相似文献   

Association between single nucleotide polymorphisms in p53 and abortion in Thoroughbred mares     

Leon PM  Campos VF  Thurow HS  Hartwig FP  Selau LP  Dellagostin OA  Neto JB  Deschamps JC  Seixas FK  Collares T 《Veterinary journal (London, England : 1997)》2012,193(2):573-575

Single nucleotide polymorphisms (SNPs) in the p53 gene have been studied extensively in humans. The aims of this study were to determine the frequency of the Arg/Pro SNP in p53 in Thoroughbred mares on one stud in Brazil and to correlate p53 genotypes with reproductive performance. SNPs were detected by PCR-restriction fragment length polymorphism in blood samples from 105 horses and confirmed by sequencing. The allele frequency in Thoroughbred mares at codon 72 in exon 4 was 73.3% Arg/Pro, 17.1% Arg/Arg and 9.6% Pro/Pro. The presence of Arg/Pro was significantly associated with abortion (P=0.02), while Pro/Pro mares had a lower probability of abortion (P<0.05). Using a logistic regression model, the dominance effect was significant (P=0.044; odds ratio 7.94) for abortion and additive effects were not significant (P=0.26). p53 may play a role in equine reproduction.  相似文献   

Developmental biology. Hox genes in the limb: a play in two acts     

Deschamps J 《Science (New York, N.Y.)》2004,304(5677):1610-1611

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Vertical Flux of Biogenic Carbon in the Ocean: Is There Food Web Control?     

RB Rivkin  L Legendre  D Deibel  JE Tremblay  B Klein  K Crocker  S Roy  N Silverberg  C Lovejoy  F Mesple  N Romero  MR Anderson  P Matthews  C Savenkoff  A Vezina  JC Therriault  J Wesson  C Berube  RG Ingram 《Science (New York, N.Y.)》1996,272(5265):1163-1166

Models of biogenic carbon (BC) flux assume that short herbivorous food chains lead to high export, whereas complex microbial or omnivorous food webs lead to recycling and low export, and that export of BC from the euphotic zone equals new production (NP). In the Gulf of St. Lawrence, particulate organic carbon fluxes were similar during the spring phytoplankton bloom, when herbivory dominated, and during nonbloom conditions, when microbial and omnivorous food webs dominated. In contrast, NP was 1.2 to 161 times greater during the bloom than after it. Thus, neither food web structure nor NP can predict the magnitude or patterns of BC export, particularly on time scales over which the ocean is in nonequilibrium conditions.  相似文献   

Soil and Sediment Geochemistry of the Iron Quadrangle, Brazil     

Eleonora Deschamps  Virginia S. T. Ciminelli  Frank Thomas Lange  J?rg Matschullat  Brigitte Raue  Hanka Schmidt 《Journal of Soils and Sediments》2004,4(3):169

Soils and sediments around gold ore deposit and mining sites in the Iron Quadrangle present positive As anomalies (median concentrations > 100 mg kg^-1) and wide ranges (< 20 to > 2000 mg kg^-1) even in densely populated areas. These anomalies can be related to geological structures, to hydrothermal ore deposits and to their continuous exploitation over the past three centuries. The paper presents new data on both As geochemistry and soil and sediment geochemistry in general, and discusses consequences of the encountered anomalies, their reasons and potential effects and how they compare with current screening and threshold values. While surface soil As-val-ues are reproducible at a given site, sediment anomalies show a strong seasonal variation that can be explained by tropical hydrologicai variances. Suggestions are presented on how to minimize the As-load in densely populated areas that might otherwise pose a potential health risk.  相似文献   

Simultaneous Measurement of Local Gain and Electron Density in X-ray Lasers     

R Cauble  Da Silva LB  TW Barbee  P Celliers  C Decker  RA London  JC Moreno  JE Trebes  AS Wan  F Weber 《Science (New York, N.Y.)》1996,273(5278):1093-1096

X-ray lasers (XRLs) have experimental average gains that are significantly less than calculated values and a persistently low level of spatial coherence. An XRL has been used both as an injected signal to a short XRL amplifier and as an interferometer beam to measure two-dimensional local gain and density profiles of the XRL plasma with a resolution near 1 micrometer. The measured local gain is in agreement with atomic models but is unexpectedly spatially inhomogeneous. This inhomogeneity is responsible for the low level of spatial coherence observed and helps explain the disparity between observed and simulated gains.  相似文献