A method has been established and validated for identification and quantification of individual, as well as total, anthocyanins by HPLC and LC/ES-MS in botanical raw materials used in the herbal supplement industry. The anthocyanins were separated and identified on the basis of their respective M(+) (cation) using LC/ES-MS. Separated anthocyanins were individually calculated against one commercially available anthocyanin external standard (cyanidin-3-glucoside chloride) and expressed as its equivalents. Amounts of each anthocyanin calculated as external standard equivalent were then multiplied by a molecular-weight correction factor to afford their specific quantities. Experimental procedures and use of a molecular-weight correction factors are substantiated and validated using Balaton tart cherry and elderberry as templates. Cyanidin-3-glucoside chloride has been widely used in the botanical industry to calculate total anthocyanins. In our studies on tart cherry and elderberry, its use as external standard followed by use of molecular-weight correction factors should provide relatively accurate results for total anthocyanins, because of the presence of cyanidin as their major anthocyanidin backbone. The method proposed here is simple and has a direct sample preparation procedure without any solid-phase extraction. It enables selection and use of commercially available anthocyanins as external standards for quantification of specific anthocyanins in the sample matrix irrespective of their commercial availability as analytical standards. It can be used as a template and applied for similar quantification in several anthocyanin-containing raw materials for routine quality control procedures, thus providing consistency in analytical testing of botanical raw materials used for manufacturing efficacious and true-to-the-label nutritional supplements. 相似文献
The ultrastructure of Katahdin tubers infected with potato virus X (PVX) is compared with PVX-free tubers by electron microscopy. Electron-dense globules surrounding the inner periphery of the tonoplast were observed in PVX-infected tubers, while PVX-free tubers did not show such bodies. Other organelles were comparable in PVX-infected and PVX-free tubers 相似文献
The effect of freezing on the turgor, ascorbic acid content and enzymatic discoloration of Katahdin and Atlantic potatoes was studied. Tubers were exposed to a temperature of ?20°C for 24 hrs. then thawed for 1 hr. at 20°C before analysis. Frozen tubers were then compared with unfrozen controls. Both varieties lost turgor following freezing and exuded a sticky liquid with increased thawing time. At the end of 3 hrs. thawing, a sponge-like texture was observed in both varieties. In both varieties freezing resulted in a significant reduction (p<.01) in ascorbic acid and phenolic content (p<.01). Enzymatic discoloration was also significantly reduced (p<.01) in both varieties. 相似文献
Genetic Resources and Crop Evolution - Loss of seed viability is a serious hurdle in production and ambient seed storage of soybean. Understanding the factors affecting seed viability, and... 相似文献
Genetic Resources and Crop Evolution - A plant species locally called ‘Kalhar’ [Blumea lacera (Burm.f.) DC.] was identified for plant genetic resources value during exploration and... 相似文献
Phosphorus (P) limitation in soil is a major concern for crop productivity. However, the use of chemical fertilizer is hazardous to the environment and costly. Therefore, the use of phosphate-solubilizing bacteria (PSB) is an eco-friendly approach for a sustainable agricultural system. In the present study, a field trial was conducted for two consecutive years to study the effects of three PSB strains isolated, Bacillus licheniformis, Pantoea dispersa, and Staphylococcus sp., with different P fertilizer rates on P uptake, P use efficiency (PUE), and grain yield of rice. The activities of soil enzymes were also studied in relation to PSB treatments. Comparative analysis of the yield and biochemical parameters revealed that inoculation of PSB consortium could reduce almost 50% of the recommended P dose in rice cultivation. Three PSB strains in combination with 50% P dose was most effective and showed the highest increases in P uptake and PUE as compared to the uninoculated control. Moreover, the PSB consortium combined with 50% P dose contributed to 50.58% and 35.64% yield increases compared to the uninoculated control for 2018 and 2019, respectively. Significant increases in the activities of soil dehydrogenase, alkaline phosphatase, and acid phosphatase were also recorded under PSB treatment. 相似文献
Journal of Plant Diseases and Protection - Potato stands first as a non-cereal food crop for global consumption purposes. During the year 2019–20, dry rot disease symptoms were observed on... 相似文献
Nitrogen and weeds are two important factors that influence the productivity of rainfed upland rice (Oryza sativa L.) in tropical Asia. A low recovery of applied fertilizer N in rainfed uplands is generally associated with high nitrate leaching losses and weed interferences. Field experiments were conducted during the wet seasons of 2002 and 2003 at the research farm of Central Rainfed Upland Rice Research Station, Hazaribag, Jharkhand, India, to determine the response of upland rice to nitrogen applied at 60 kg N ha–1 as different forms of urea (single pre‐plant application of controlled‐release urea, single pre‐plant application of urea supergranules, and split application of prilled urea with or without basal N) against no N application under three weed‐control regimes (unweeded, pre‐emergence application of butachlor at 1.5 kg a.i. ha–1 supplemented with one single hand weeding or two hand weedings). The response of rice to applied N varied greatly among the three weed‐control regimes. Across the different N treatments, the application of 60 kg N ha–1 resulted in a grain‐yield increase above the unfertilized control of only 0.24 Mg ha–1 in unweeded treatments, whereas yields increased by 1.07 Mg ha–1 when butachlor application was supplemented with a single hand weeding and by 1.28 Mg ha–1 with two hand weedings. Among the weed‐control measures, hand weeding twice produced highest grain yield in both years. The comparison of different forms of urea showed that a single pre‐plant application of controlled‐release urea resulted in average grain yields of 1.57 and 1.87 Mg ha–1 compared to 1.32 and 1.30 Mg ha–1 in the case of the recommended practice of split‐applied prilled urea in the years 2002 and 2003, respectively. The highest agronomic N use efficiency of 15–20 kg grain per kg N applied and the highest apparent N recovery of 39%–45% were attained with controlled‐release urea, suggesting that this N form is particularly beneficial for upland‐rice cultivation under variable rainfall conditions, provided weeds are controlled. 相似文献
Characterization of tannery sludge (TS) for its plausible use in amelioration and phytoremediation of heavy metal rich TS treated soil by growing economically important plants (Ricinus communis, Brassica juncea and Nerium oleander).
Materials and methods
Treatments were prepared by amalgamation of TS (0, 5, 10, 20, 30, 50, 75, 100 %) with garden soil (GS). All treatments were analysed for chemical properties, total and DTPA (Diethylene triamine pentaacetic acid) extractable heavy metals (Cr, Pb, Cu, and Mn). Seed germination experiment was conducted; unvarying saplings were selected and planted in concerned pots and allowed to grow for 90 days in green house. At harvest, plant samples were washed with distilled water and used for determination of growth parameters (biomass of root, shoot and total biomass on dry weight basis) and metal accumulation in different parts of the plant. Translocation factor (TF) and bioconcentration factor (BCF) have been calculated to check the phytostabilization capability of studied plants.
Results and discussion
Application of TS in fixed quantity as an amendment resulted in significant improvements of GS characteristics (alkaline pH with high electrical conductivity, organic carbon, available NPK and heavy metals) and in treatments. DTPA extractable heavy metal concentrations were found very low and total heavy metal concentrations were also found under allowable range in control and treated soil (T-I to T-VI). The maximum seed germination percentage, plant growth, biomass production for all plant species were observed in T-III treatment (20 % TS + 80 % GS) with majority of the metal accumulation in underground part (BCF >1) and meagre translocation in aerial part (TF <1). From T-IV to T-VII treatment, accumulation of heavy metals in plant parts has generally increased; however, biomass has been tremendously decreased.
Conclusions
TS was found rich in NPK content with significant concentration of heavy metals. Pot growth experiment suggested amelioration of GS with specific quantity of 20 % TS can tremendously enhance the plant growth, help in the utilization of TS and can act as a substitute of synthetic fertilizer. Majority of the metals was accumulated in root part (BCF >1) and meagre translocation (TF <1) in aerial part, concludes R. communis and B. juncea could be suitable plant species to be grown in heavy metal rich TS treated soil, vis-à-vis for phytostabilization of heavy metals. In addition, these oil yielding and medicinal plants can also be used for phytoremediation of moderately contaminated tannery soils.
The aim of this study was to understand impacts of long-term (43 years) fertilization on soil aggregation, N accumulation rates and δ15N in surface and deep layers in an Alfisol. Soil samples from seven treatments were analysed for N stocks, aggregate-associated N in 0–30 cm and the changes in δ15N in 0–90 cm depths. The treatments were: unfertilized control (control); recommended N dose (N); recommended N and phosphorus doses (NP); recommended N, P and potassium doses (NPK); 150% of recommended N, P and K doses (150% NPK); NPK + 10 Mg FYM ha?1 (NPK + FYM) and NPK + 0.4 Mg lime ha?1 (NPK + L). Results revealed that plots under NPK + FYM had ~39% higher total N concentrations than NPK + L in 0–30 cm soil layers. In NPK + L, macro-aggregates had 35 and 11% and microaggregates had 20 and 9% lower δ15N values than NPK + FYM in 0–15 and 15–30 cm soil layers, respectively. However, plots receiving NPK + FYM had ~39% greater deep soil (30–90 cm) N accumulation than NPK + L. These results would help understanding N supplying capacity by long-term fertilization and assist devising N management strategies in sub-tropical acidic Alfisols. 相似文献
