Identification and pathogenicity study of emerging fish pathogens Acinetobacter junii and Acinetobacter pittii recovered from a disease outbreak in Labeo catla (Hamilton, 1822) and Hypophthalmichthys molitrix (Valenciennes, 1844) of freshwater wetland in West Bengal,India     

Ramesh Chandra Malick  Asit Kumar Bera  Hemanta Chowdhury  Manojit Bhattacharya  Tanuja Abdulla  Himanshu Sekhar Swain  Raju Baitha  Vikas Kumar  Basanta Kumar Das 《Aquaculture Research》2020,51(6):2410-2420

The disease outbreaks in aquaculture system of wetlands are the major cause of fish mortality. Among various bacterial septicaemic diseases, fish mortality caused by Acinetobacter spp. is recently reported in different fish species. Fish disease outbreak was investigated in a wetland of West Bengal, India to identify the aetiological factors involved. The moribund fish were examined and subjected to bacterial isolation. Two bacterial causative agents were identified as Acinetobacter junii and Acinetobacter pittii by biochemical characterization and 16S rRNA gene amplification. Both the isolates were oxidase‐negative, nitrate‐negative, catalase‐positive and indole‐negative. The molecular identification using 16S rRNA gene sequencing and phylogenetic tree analysis further confirmed the two Acinetobacter spp. with 97%–99% similarity. The antibiotic resistance patterns of these two bacteria revealed that both of them were resistant to β‐lactam, cefalexin, cephalothin, amoxyclav, cefuroxime, cefadroxil, clindamycin, vancomycin and penicillin. In addition, A. pittii was also resistant to other antibiotics of cephams group such as ceftazidime and cefotaxime. In the challenge experiment, both A. junii and A. pittii were found to be pathogenic with LD₅₀ of 1.24 × 10⁵ and 1.88 × 10⁷ cfu/fish respectively. Histopathological examination of gill, liver and kidney revealed prominent changes supporting bacterial septicaemia. The investigation reports for the first time on concurrent infection by A. junii and multidrug‐resistant (MDR)‐A. pittii as emerging fish pathogens to cause severe mortality in Labeo catla and Hypophthalmichthys molitrix in a freshwater wetland.  相似文献   

Fecundity and egg dimension of freshwater prawn Macrobrachium assamense peninsulare (Tiwari, 1958) from Rawasan stream of Garhwal,Central Himalaya,India     

Koshal Kumar  Chandra Bhanu Kotnala  Anita Rawat Rana 《Aquaculture Research》2019,50(12):3608-3615

The population of the freshwater animal was declined day by day due to the degradation of habitat and use of illegal fishing methods, which cause a great threat to these aquatic animals. The main purpose of this study was to scrutinize the wild population and fecundity of freshwater prawn Macrobrachium assamense peninsulare in Rawasan stream to assess the health status of prawn. Fecundity was estimated by a random sampling of prawn from five selected sites of Rawasan stream. A wild collection of a total of 35 ovigerous females was collected for an interval of 2 years (August 2013–July 2015) from May to September month, that is breeding session. The maximum number of eggs was 102 eggs in an individual having 52 mm total length and 2.66 g body weight. It was concluded that fecundity of Macrobrachium assamense peninsulare was low, and egg dimension was larger than other closely related species of freshwater prawn. Fecundity of Macrobrachium assamense peninsulare showed a positive relationship with a total weight (R² = .71) and moderate with the total length (R² = .38). A strong correlation exists between egg mass and total weight, and between egg mass and total length of female (R² = .849 and R² = .77 at p < .05) respectively. The numbers of eggs, that is fecundity, in a female were increased with the body growth of an individual, which results in a linear graph.  相似文献   

Identification of potential sources of mungbean yellow mosaic India virus resistance in black gram (Vigna mungo) and expression of antioxidants and R-genes modulating resistance response in cultivated and its two wild relatives     

Anupam Tripathi  Chandra Mohan Singh  Mukul Kumar  Shalini Purwar  Anuj Mishra  Dharmendra Kumar  Awnindra Kumar Singh  Sujit Kumar  Sanjay Singh  Narendra Pratap Singh 《Plant Breeding》2023,142(5):668-681

Black gram is one of the most important short duration grain legume, which contributes significantly towards nutritional security and environmental sustainability. The virus specific primers confirms the presence of mungbean yellow mosaic India virus (MYMIV) in representative samples. A total of 27 cultivated and two wild species were found as highly resistant (HR) to MYMIV and validated through molecular markers. The start codon target (SCoT) markers analysis revealed that the SCoT loci, namely, SCoT-4 (2200 bp), SCot-9 (1150/ 1200 bp), SCoT-15 (1150/1100 bp), SCoT-16 (700 bp), SCoT-24 (2500 bp), SCoT-25 (700 bp), SCoT-33 (900/1000 bp), and SCoT-34 (600 bp), were found unique, able to distinguish HR and highly susceptible (HS) genotypes. Biochemical characterization and gene expression profiling revealed the higher expression of antioxidants and R-genes just after pathogen inoculation indicated the activation of defence mechanism in both cultivated and its wild relatives, which modulates the resistant responses in cultivated and wild accessions. These information will be really helpful in accelerating resistance breeding in black gram.  相似文献   

Exploration of ready-to-eat soft Bora rice genotypes of Assam for submergence tolerance     

Panja  Suraj  Mondal  Kongkong  Kar  Rup Kumar  Dey  Pradip Chandra  Dey  Narottam 《Journal of Crop Science and Biotechnology》2023,26(1):87-95

Journal of Crop Science and Biotechnology - Assamese Bora rice lines are valuable genetic resource for their socio-economic importance and traditional usage. Being lowland rice lines of upper...  相似文献   

Livelihood Improvements Through Agroforestry: Evidence from Northern Bangladesh     

Md. Abu Hanif  Ranjan Mitra Roy  Md. Shafiqul Bari  Polash Chandra Ray  Md. Shoaibur Rahman  Md. Faruq Hasan 《Small-Scale Forestry》2018,17(4):505-522

Agroforestry is one of the most sustainable land management systems practiced around the world due to the socioeconomic benefits that it brings to farmers. In Bangladesh, farmers practice agroforestry, applying indigenous knowledge. The present study was designed to identify the present status, management practices and its role in improving the livelihoods of farmers in northern Bangladesh. Data for the study were collected through quantitative and qualitative methods. A total of 29 tree and 38 agricultural crop species were planted by the102 farmers interviewed. Mangifera indica (relative prevalence 49%) is the most predominant species, followed by Eucalyptus camaldulensis (relative prevalence 35.4%). Farmers of northern Bangladesh plant trees in cropland for fruits (90%), fuel wood (87%) and timber production (79%). Fruit trees were planted with wider spacing while forest and fuel wood species were planted with narrower spacing. Farmer’s livelihoods improved enormously by practicing agroforestry as they have more access to food, fodder and fuel wood which is reflected by greater access to livelihood capitals (except social capital). However, farmers have experienced increased incidences of pests and diseases to the annual crops and trees. Agroforestry practices increases species diversity, ensure economic return and sustain farmer’s livelihoods.  相似文献   

Heterologous expression of Lolium perenne antifreeze protein confers chilling tolerance in tomato     

Srinivasan Balamurugan  Jayan Susan Ann  Inchakalody P Varghese  Shanmugaraj Bala Murugan  Mani Chandra Harish  Sarma Rajeev Kumar  Ramalingam Sathishkumar 《农业科学学报》2018,17(5):1128-1136

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Informative ISSR Markers Help Identify Genetically Distinct Accessions of Oryza rufipogon in Yield Improvement     

Haritha G.  Sudhakar T.  Chandra D.  Ram T.  Divya B.  Sarla N. 《水稻科学》2016,23(5):225

Inter simple sequence repeat(ISSR) polymorphism was used to determine genetic diversity and phylogenetic relationships in 90 genotypes of wild and cultivated species of Oryza from different geographical regions of the world. In all the 17 primers used in ISSR-PCR, a total of 11 464 bands were amplified at 253 band positions/loci. The primer UBC-809 amplified the maximum bands(1 059) at 21 band positions. UBC-810 and UBC-835 amplified the minimum of 391 bands each at 7 and 14 band positions, respectively. The mean polymorphism information content ranged from 0.44 to 0.84 and resolving power ranged from 8.69 to 23.53. Un-weighted pair group method with arithmetic mean dendrogram and population structure based on the 17 primers separated all genotypes into 4 major clusters with a genetic similarity of 53%–100%. The first two clusters consisted of 30 O. rufipogon accessions each. In the third cluster, O. nivara and O. longistaminata grouped as one sub-cluster and all other O. nivara accessions and cultivars grouped as another sub-cluster. The fourth cluster had only five O. rufipogon accessions which can be a source of new genes. Four sub-populations were identified within O. rufipogon and two sub-populations within O. nivara at K = 7. A subset of six primers with high resolving power values were the most informative and grouped all genotypes almost similarly as the 17 primers did. Use of these six highly informative primers in ISSR-PCR is a cost effective and robust method for assessing genetic diversity in large germplasm collections of wild rice species.  相似文献   

Diversity of bacterial communities in the midgut of Bactrocera cucurbitae (Diptera: Tephritidae) populations and their potential use as attractants          下载免费PDF全文

Ashok B Hadapad  Chandra S Prabhakar  Snehal C Chandekar  Jyoti Tripathi  Ramesh S Hire 《Pest management science》2016,72(6):1222-1230

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New source of black rot disease resistance in Brassica oleracea and genetic analysis of resistance     

Partha Saha  Pritam Kalia  Munish Sharma  Dinesh Singh 《Euphytica》2016,207(1):35-48

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Identification of quantitative trait loci controlling sucrose content based on an enriched genetic linkage map of sugarcane (Saccharum spp. hybrids) cultivar ‘LCP 85-384’     

Pingwu Liu  Amaresh Chandra  Youxiong Que  Ping-Hua Chen  Michael P. Grisham  William H. White  Caleb D. Dalley  Thomas L. Tew  Yong-Bao Pan 《Euphytica》2016,207(3):527-549

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