Pathogen Analysis of Goat's Respiratory Diseases     

TAO Li  LAN Xian-li  LI Jun  MA Chun-xia  LAN Jin-hong  HUANG Ming-xue  LAN Mei-yi  BI Bing-fen  CHEN Ze-xiang  YANG Wei 《中国畜牧兽医》2016,43(4):1072-1078

To investigate the pathogens of goat's respiratory disease from a goat farm in Guangxi province, the epidemiological investigation, clinic observation, pathological examination, bacterium isolation and identification, biochemical test, PCR and animal experiment test were conducted.The preventive and control measures were taken on the basis of pathogen epidemiological characteristics and drug sensitive test results.The results showed that Mycoplasma, influenza virus and parasite were negative by isolation and culture or PCR, however, two strains of gram-negative bacteria named MS1 and MS2 were isolated from lung.MS1 was identified as Serratia marcescens by biochemical test and 16S rRNA sequencing, which shared 99% nucleotide homology with other Serratia marcescens in GenBank.And MS2 was identified as E.coil by the same methods that shared 99% nucleotide homology with other E.coli in GenBank.Animal experiment showed that two strains could cause death in mice.The drug sensitive tests showed two strains were highly sensitive to spectinomycin, amikacin, kanamicin and neomycin.Kanamycin and dexamethasone were used to treat the sick goats, and achieved good results.  相似文献   

Effects of Dietary Supplementation of Barodon on Growth Performance,Innate Immunity and Disease Resistance of Juvenile Olive Flounder,Paralichthys olivaceus,Against Streptococcus iniae     

Chang‐Hoon Shin  Hien Thi Dieu Bui  Samad Rahimnejad  Ji‐Hoon Cha  Byung‐Woo Yoo  Bo‐Kyeun Lee  Hyung‐Jin Ahn  Soo‐Il Choi  Yun‐Jeong Choi  Yong‐Ho Park  Jeong‐Dae Kim  Kyeong‐Jun Lee 《Journal of the World Aquaculture Society》2014,45(3):258-268

This study was conducted to evaluate the effects of dietary supplementation of Barodon, an anionic alkali mineral complex, on growth, feed utilization, humoral innate immunity and disease resistance of olive flounder. A basal experimental diet was used as a control and supplemented with 0.1, 0.2, 0.3, 0.4, or 0.5% Barodon. Triplicate groups of fish (26.4 ± 0.2 g) were fed one of the diets to apparent satiation twice daily for 10 wk. The growth performance was enhanced (P < 0.05) linearly and quadratically in fish fed diets containing Barodon compared with that in fish fed the control. Feed utilization was significantly improved by Barodon supplementation. Serum lysozyme and antiprotease activities were increased quadratically in Barodon fed groups. Also, significantly higher superoxide dismutase activity was found in Barodon‐fed fish. Dietary supplementation of 0.1–0.3% Barodon resulted in significant enhancement of fish disease resistance against Streptococcus iniae. The findings in this study indicate that dietary supplementation of Barodon can enhance growth, feed utilization, innate immunity, and disease resistance of olive flounder and that the optimum level seems to be 0.1% in diets.  相似文献   

Preparation of Atherosclerosis Model Using Guangxi Bama Mini-pig     

LI Yan-jun  SONG Shao-rui  GUO Ya-fen  LAN Gan-qiu  CHEN Bao-jian  YAN Xue-yu  WU Yan-jun  JIANG Qin-yang 《中国畜牧兽医》2015,42(7):1770-1776

The atherosclerosis (AS) model was prepared in Guangxi Bama mini-pig,and the atherosclerosis index (AI) was preliminarily identified in occurrence of AS to provide the basis for the preparation of related models.20 Guangxi Bama mini-pigs were randomly divided into control group and experimental group which were fed with high fat and cholesterol diet to prepare the AS model.Blood biochemical indexes were detected in the process of model preparation and the correlation between AI and the results of vascular slices was analyzed to initially draw up AI of AS.The results of vascular slices showed that the incidence rates of AS of Guangxi Bama mini-pigs in experimental group and control group were 20% and 0,respectively.The association analysis between the results of vascular slices and AI in the pathogenic Guangxi Bama mini-pigs preliminarily suggested that AI was above 3.8 and lasted for more than 3 months during the attack.  相似文献   

不同培养基成分对薄荷试管苗生根的影响     

兰伟  韩晴 《安徽农业科学》2015,(14)

[目的]筛选薄荷试管苗生根的最佳培养基组合。[方法]以薄荷试管苗茎段为试验材料,探讨不同培养基成分、凝固剂种类及添加量对其生根的影响。[结果]在薄荷试管苗培养过程中,使用琼脂为凝固剂,且添加量为7 g/L的1/2MS培养基时,其生根时间最短,生根数和生根长度较高。[结论]为薄荷试管苗的大规模、优质、高效生产提供理论依据。  相似文献   

不同播期和种植方式对糯玉米相关性状及产量的影响     

李青超  王立达  赵秀梅  韩业辉  刘洋  兰红宇  杨莹 《黑龙江农业科学》2020,(9):20-23

  相似文献   

茂兰喀斯特森林溪流蜻蜓目昆虫资源调查初报     

兰洪波  冉景丞 《湖北农业科学》2014,(7):1528-1530,1534

通过调查和鉴定,从茂兰喀斯特森林溪流采集到蜻蜓63种,隶属11科,其中,蜻科25种,春蜓科4种,蟌科7种,蜓科6种,扇蟌科5种,大蜻科2种,色蟌科3种,溪蟌科4种,丽蟌科2种,隼蟌科4种,山蟌科1种;跳长尾蜓、黑尾灰蜻、黑丽翅蜻、红痣绿色蟌、大溪蟌、点斑隼蟌、白腹小蟌属于贵州新记录种。  相似文献   

天然杨桦用材林高效经营技术研究     

兰士波 《安徽林业》2011,(3)

选择不同林分类型和立地条件,按不同经营密度分别设立固定标准地,开展立地类型划分和最适密度选择。同时,在固定标准地内,对林分生长性状、形质指标、干物质积累量、枯枝落叶分解量及养分归还量进行连续定位观测。结果表明:经营密度对林分生长、干物质积累和枯枝落叶分解影响较大,且以中等经营密度(0.7)效果最理想。在立地类型划分、最适经营密度选择、密度效应分析、径级-株数分布规律研究的基础上,针对不同培育目标,分别提出不同立地条件下天然杨桦(胶合板材、普通建筑材、纸浆材)用材林高效经营技术,为科学经营存量种质资源,最大限度提高森林生产力奠定理论基础。  相似文献   

广西东门林场森林资源现状和可持续发展策略   总被引：1，自引：0，他引：1  

莫继有  吴永富  兰俊  黎怀玲  吴兵  邓海群 《桉树科技》2011,(1):53-56

根据广西东门林场2009年森林资源二类调查的结果,分析东门林场森林资源现状、特点及存在的问题,并提出东门林场森林资源可持续发展的策略。  相似文献   

皇后黑李优良单株——隆丰1号黑李的选育     

魏泽平  兰秀英  陈兰进 《闽东农业科技》2011,(2)

对皇后黑李、隆丰1号黑李的生物学特征和植物学特性进行观察记载;开展皇后黑李优良单株的选育,选出优良单株——隆丰1号黑李。  相似文献   

金针菇的研究进展及开发利用前景     

杨万波  兰士波  温爱亭  李红艳  马盈 《安徽林业》2013,(1):41-43

金针菇是颇受世人青睐的食用菌之一,具有较高的营养价值、药用价值和保健功效。在汲取国内外相关研究成果及学术观点的基础上,详细阐述了金针菇的营养价值和保健功效,以及引种驯化、遗传育种和杂交育种等领域的国内外研究进展;指出了开发利用方面存在的不足,展望了资源开发和利用的前景。  相似文献