Digested soybean protein and taurine influence bile acid level,lipase activity,lipid digestibility,and growth performance of pompano (Trachinotus blochii)     

Nguyen  Hung Phuc  Van Do  Thinh 《Fish physiology and biochemistry》2021,47(4):1199-1209

Fish Physiology and Biochemistry - This study aimed to examine the effects of dietary digested soybean protein (DSP) and taurine on bile acid (BA) level, lipase activity, lipid apparent...  相似文献   

Expression of neurotrophic factors in injured spinal cord after transplantation of human-umbilical cord blood stem cells in rats     

Hyo-jin Chung  Wook-hun Chung  Jae-Hoon Lee  Dai-Jung Chung  Wo-Jong Yang  A-Jin Lee  Chi-Bong Choi  Hwa-Seok Chang  Dae-Hyun Kim  Hyun Jung Suh  Dong-Hun Lee  Soo-Han Hwang  Sun Hee Do  Hwi-Yool Kim 《Journal of veterinary science (Suw?n-si, Korea)》2016,17(1):97-102

We induced percutaneous spinal cord injuries (SCI) using a balloon catheter in 45 rats and transplanted human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) at the injury site. Locomotor function was significantly improved in hUCB-MSCs transplanted groups. Quantitative ELISA of extract from entire injured spinal cord showed increased expression of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF) and neurotrophin-3 (NT-3). Our results show that treatment of SCI with hUCB-MSCs can improve locomotor functions, and suggest that increased levels of BDNF, NGF and NT-3 in the injured spinal cord were the main therapeutic effect.  相似文献   

Isolation and antifungal activity of lignans from Myristica fragrans against various plant pathogenic fungi     

Cho JY  Choi GJ  Son SW  Jang KS  Lim HK  Lee SO  Sung ND  Cho KY  Kim JC 《Pest management science》2007,63(9):935-940

BACKGROUND: In a search for plant extracts with potent in vivo antifungal activity against various plant diseases, we found that treatment with a methanol extract of Myristica fragrans Houttyn (nutmeg) seeds reduced the development of various plant diseases. The objectives of the present study were to isolate and determine antifungal substances from My. fragrans and to evaluate their antifungal activities. RESULTS: Three antifungal lignans were isolated from the methanol extract of My. fragrans seeds and identified as erythro-austrobailignan-6 (EA6), meso-dihydroguaiaretic acid (MDA) and nectandrin-B (NB). In vitro antimicrobial activity of the three lignans varied according to compound and target species. Alternaria alternata, Colletotrichum coccodes, C. gloeosporioides, Magnaporthe grisea, Agrobacterium tumefaciens, Acidovorax konjaci and Burkholderia glumae were relatively sensitive to the three lignans. In vivo, all three compounds effectively suppressed the development of rice blast and wheat leaf rust. In addition, EA6 and NB were highly active against the development of barley powdery mildew and tomato late blight, respectively. Both MDA and NB also moderately inhibited the development of rice sheath blight. CONCLUSION: This is the first study to demonstrate the in vitro and in vivo antifungal activities of the three lignans from My. fragrans against plant pathogenic fungi.  相似文献   

PI(3,4,5)P3 and PI(4,5)P2 lipids target proteins with polybasic clusters to the plasma membrane     

Heo WD  Inoue T  Park WS  Kim ML  Park BO  Wandless TJ  Meyer T 《Science (New York, N.Y.)》2006,314(5804):1458-1461

Many signaling, cytoskeletal, and transport proteins have to be localized to the plasma membrane (PM) in order to carry out their function. We surveyed PM-targeting mechanisms by imaging the subcellular localization of 125 fluorescent protein-conjugated Ras, Rab, Arf, and Rho proteins. Out of 48 proteins that were PM-localized, 37 contained clusters of positively charged amino acids. To test whether these polybasic clusters bind negatively charged phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] lipids, we developed a chemical phosphatase activation method to deplete PM PI(4,5)P2. Unexpectedly, proteins with polybasic clusters dissociated from the PM only when both PI(4,5)P2 and phosphatidylinositol 3,4,5-trisphosphate [PI(3,4,5)P3] were depleted, arguing that both lipid second messengers jointly regulate PM targeting.  相似文献   

Evolution and functional impact of rare coding variation from deep sequencing of human exomes     

Tennessen JA  Bigham AW  O'Connor TD  Fu W  Kenny EE  Gravel S  McGee S  Do R  Liu X  Jun G  Kang HM  Jordan D  Leal SM  Gabriel S  Rieder MJ  Abecasis G  Altshuler D  Nickerson DA  Boerwinkle E  Sunyaev S  Bustamante CD  Bamshad MJ  Akey JM;Broad GO;Seattle GO;NHLBI Exome Sequencing Project 《Science (New York, N.Y.)》2012,337(6090):64-69

As a first step toward understanding how rare variants contribute to risk for complex diseases, we sequenced 15,585 human protein-coding genes to an average median depth of 111× in 2440 individuals of European (n = 1351) and African (n = 1088) ancestry. We identified over 500,000 single-nucleotide variants (SNVs), the majority of which were rare (86% with a minor allele frequency less than 0.5%), previously unknown (82%), and population-specific (82%). On average, 2.3% of the 13,595 SNVs each person carried were predicted to affect protein function of ~313 genes per genome, and ~95.7% of SNVs predicted to be functionally important were rare. This excess of rare functional variants is due to the combined effects of explosive, recent accelerated population growth and weak purifying selection. Furthermore, we show that large sample sizes will be required to associate rare variants with complex traits.  相似文献   

Meiotic stability in two valuable interspecific hybrids of Brachiaria (Poaceae)     

Mariana F. Felismino  Maria S. Pagliarini  Cacilda B. Do Valle  Rosângela. M. S. Resende 《Plant Breeding》2012,131(3):402-408

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Silk fibroin hydrolysate exerts an anti-diabetic effect by increasing pancreatic β cell mass in C57BL/KsJ-db/db mice     

Sun-Gil Do  Jun-Hong Park  Hajin Nam  Jin-Bong Kim  Jae-Yong Lee  Yang-Seok Oh  Jun-Gyo Suh 《Journal of veterinary science (Suw?n-si, Korea)》2012,13(4):339-344

Components of silk including silk fibroin have long been used as anti-diabetic remedies in oriental medicine. However, detailed mechanisms underlying these anti-diabetic effects remain unclear. In this study, we examined the anti-diabetic activity of silk fibroin hydrolysate (SFH) in C57BL/KsJ-db/db (db/db) mice, a well-known animal model of non-insulin dependent diabetes mellitus. When the db/db mice were administered SFH in drinking water for 6 weeks, hyperglycemia in the animals gradually disappeared and the level of glycosylated hemoglobin decreased, indicating that SFH plays important role in reducing the symptoms of diabetes. In addition, SFH-treated db/db mice exhibited improved glucose tolerance with increased plasma insulin levels. Immunohistochemical and morphological analyses showed that SFH up-regulated insulin production by increasing pancreatic β cell mass in the mice. In summary, our results suggest that SFH exerts anti-diabetic effects by increasing pancreatic β cell mass in a non-insulin dependent diabetes mellitus mouse model.  相似文献   

Effects of application for international quality assurance certifications on the farm’s profit: evidence from Vietnamese shrimp     

Dong  Khuu Thi Phuong  Duc  Nguyen Minh  Hoa  Nguyen Thi Ngoc  Hanh  Bui Le Thai  Trang  Le Thi Thu 《Aquaculture International》2021,29(3):1265-1277

Aquaculture International - Food incidents have led to the increase in demand for food products certified by an international quality assurance certification of consumers all over the world. Shrimp...  相似文献   

Ileal and total tract digestibility in local (Mong Cai) and exotic (Landrace × Yorkshire) piglets fed low and high-fibre diets, with or without enzyme supplementation     

Ninh Thi Len  Tran Bich Ngoc  Brian Ogle  Jan Erik Lindberg   《Livestock Science》2009,126(1-3):73-79

Ileal and total tract digestibility of low and high-fibre diets (100 and 200 g/kg neutral detergent fibre), with or without enzyme supplementation (mixture of cellulase, β-glucanase, α-amylase and protease) was determined in piglets of two breeds weaned at 30 days of age. The breeds were local (pure-breed Mong Cai, MC) and exotic (Landrace × Yorkshire, LY). The experimental diets were based on maize meal, soybean meal, fish meal, cassava residue meal, and rice bran. Cassava residue meal and rice bran were the main fibrous feeds in the high-fibre diet. The experiment was arranged according to a 2 × 2 × 2 factorial, completely randomized design with 4 replications, and lasted for 30 days. The coefficient of ileal apparent digestibility (CIAD) was measured by analyzing ileal digesta collected from piglets killed at 60 days of age. The coefficient of total tract apparent digestibility (CTTAD) was measured by analyzing faecal samples collected for 5 consecutive days immediately before the experiment finished. The CIAD and CTTAD of organic matter, crude protein, crude fibre, neutral detergent fibre, acid detergent fibre and amino acids in the high-fibre diet were lower than in the low-fibre diet (P < 0.01). The CIAD of nutrients and amino acids was similar (P > 0.05) between MC and LY, while the CTTAD of nutrients was higher for the MC than for LY (P < 0.05). Enzyme supplementation of the high-fibre diet improved daily gain and the digestibility of all dietary components at the ileum and in the total tract (P < 0.05), but there was no effect of enzyme supplementation of the low-fibre diet (P > 0.05).  相似文献   

Using new egg parasitoids (Trichogramma spp.) to improve integrated management against Tuta absoluta     

H. Do Thi Khanh  A. Chailleux  M. Tiradon  N. Desneux  E. Colombel  E. Tabone 《EPPO Bulletin》2012,42(2):249-254

This study is the first step of the TutaPI program (2011–13) to decrease the costs of Integrated Pest Management (IPM) strategies against Tuta absoluta in France by investigating new beneficial insects. Currently, the association of predator/egg parasitoid (Macrolophus pygmaeus and Trichogramma achaeae, respectively) in IPM strategies are quite effective, but expensive. Sixty‐four new potential strains from 19 Trichogramma species originating from different regions of the world (from the collection of Trichogramma at INRA Provence Alpes Côte d'Azur (PACA), France) were studied in this work. A screening study was performed on T. absoluta eggs on isolated tomato leaves (for 24 h at 25 ± 1°C, RH 75 ± 5% and 16 h light : 8 h dark). The proportion of parasitized T. absoluta eggs varied greatly (between 0 and 73%) depending on the different strains used. Levels of parasitism by females, equal to or higher than that of T. achaeae, were observed in 14 of the strains tested among eight species (four from France, four from elsewhere in Europe). The proportion of females that parasitized T. absoluta eggs was significantly different between strains (from 0 to 100%). Experimental results allowed about half of the 64 initial strains to be eliminated before the next steps: tests on a larger scale (mesocosm, and then in the greenhouse and field).  相似文献