Analysis of serum proteins and cerebrospinal fluid in clinically normal horses, using agarose electrophoresis.     

F Kristensen  E C Firth 《American journal of veterinary research》1977,38(7):1089-1092

Using agarose as a supporting matrix, electrophoresis was conducted on 50 serum samples and 20 cerebrospinal fluid samples from clinically normal horses (n = 50) of various ages and breeds. The technique was shown to be reliable. A positive correlation between age and gamma-globulin concentration was found in young horses. Features of the electrophoretograms of serum and cerebrospinal fluid samples are discussed, and a nomenclature based on Rf values is proposed.  相似文献   

Changes in mineralised tissue at the site of origin of condylar fracture are present before athletic training in Thoroughbred horses     

EC Firth  M Doube  A Boyde 《New Zealand veterinary journal》2013,61(5):278-283

Abstract

AIM: To show that changes are present at the site of origin of metacarpal condylar fracture in young Thoroughbred horses before they enter race training.

METHOD: Bone slices, 2 mm thick, in three mediolateral planes through the centre of rotation of the metacarpo-phalangeal joint (MCPJ) of both distal third metacarpal bones (Mc3) of 12 Thoroughbred horses aged 17 months, were imaged using point-projection digital X-ray imaging (µXR).

RESULTS: In some horses, linear or ovoid radiolucency was found in articular calcified cartilage (ACC) and subchondral bone of the palmaro-distal aspect of the sagittal groove, exactly at the site of more advanced stages of condylar fatigue fracture. An incidental finding was ovoid radiolucency in the apex of the dorso-distal aspect of the sagittal ridge, with or without fragmentation or disturbance of the subchondral mineralised tissue line, resembling equine osteochondrosis.

CONCLUSIONS AND CLINICAL RELEVANCE: The findings imply that the aetiology of condylar fatigue fracture in young Thoroughbred horses includes abnormality in development of the bone and joint that is present before athletic activity occurs.  相似文献   

An overlapping protein-coding region in influenza A virus segment 3 modulates the host response   总被引：1，自引：0，他引：1  

Jagger BW  Wise HM  Kash JC  Walters KA  Wills NM  Xiao YL  Dunfee RL  Schwartzman LM  Ozinsky A  Bell GL  Dalton RM  Lo A  Efstathiou S  Atkins JF  Firth AE  Taubenberger JK  Digard P 《Science (New York, N.Y.)》2012,337(6091):199-204

Influenza A virus (IAV) infection leads to variable and imperfectly understood pathogenicity. We report that segment 3 of the virus contains a second open reading frame ("X-ORF"), accessed via ribosomal frameshifting. The frameshift product, termed PA-X, comprises the endonuclease domain of the viral PA protein with a C-terminal domain encoded by the X-ORF and functions to repress cellular gene expression. PA-X also modulates IAV virulence in a mouse infection model, acting to decrease pathogenicity. Loss of PA-X expression leads to changes in the kinetics of the global host response, which notably includes increases in inflammatory, apoptotic, and T lymphocyte-signaling pathways. Thus, we have identified a previously unknown IAV protein that modulates the host response to infection, a finding with important implications for understanding IAV pathogenesis.  相似文献   

A preliminary investigation of the use of a foal image to reduce mare stress during mare–foal separation     

Chris W. Rogers  Victoria Walsh  Erica K. Gee  Elwyn C. Firth 《Journal of Veterinary Behavior: Clinical Applications and Research》2012,7(1):49-54

The data for this study were collected from 7 mares (10 ± 1.6 years old) when their foals were 4-, 15-, 30-, 45-, or 60-days-old. On each occasion, the foals were anesthetized and placed out of sight of the mare and the mares’ behavior, heart rate, and cortisol response to foal separation in either the presence of a foal phantom, a square shape, or no visual stimuli were measured. In the presence of the foal phantom, anxious (P = 0.119) and agitated (P = 0.524) mare behavior was not significantly different from baseline values. The mares had significantly elevated behavior scores for 5 out of 6 criteria when the square phantom was presented and were significantly more active (higher scores for fidgety and active criteria) when no visual stimuli (control) were provided. Based on the data presented in this study, the use of a phantom image of a foal with neutral ear and tail position may be an effective nonchemical method of calming mares during separation from their foal.  相似文献   

Risk factors for interruptions to training occurring before the first trial start of 2-year-old Thoroughbred racehorses     

Bolwell CF  Rogers CW  French NP  Firth EC 《New Zealand veterinary journal》2012,60(4):241-246

  相似文献   

The quantitative assessment of photodensity of the third carpal bone in the horse     

Secombe CJ  Firth EC  Perkins NR  Bailey D  Anderson BH 《New Zealand veterinary journal》2004,52(2):70-75

AIM: To determine whether variation in x-ray-beam angle significantly affected photodensity of the third carpal bone (C3) in the horse using the tangential radiographic view, and indirectly determine whether radioabsorptiometry (RA) could be used to assess differences in bone mineral density (BMD) of C3 between exercised and non-exercised horses. METHODS: The left distal carpal row was isolated post-mortem from 2-year-old Thoroughbred horses that had been either exercised (n=7) for up to 14 weeks using a standard training regimen for racehorses, or not exercised (n=7). The effect of variation in x-ray-beam angle on photodensity was determined on these isolated carpal bones in vitro. Image analysis was used to assess photodensity (compared to a known thickness of aluminium) of four regions of interest (ROI) in C3 and one ROI in the fourth carpal bone (C4) of 14 isolated distal rows of carpal bones of the horse. The isolated carpal bones were placed fl at on a x-ray cassette and radiographed at 90 degrees (i.e. with the x-ray beam perpendicular to the cassette). The x-ray-beam angle was varied in the dorsal sagittal plane by 5 degrees increments to a total of 15 degrees from 90 degrees and from a base angle of 60 degrees (the x-ray-beam angle at which the tangential view is taken in clinical cases). RESULTS: Variation in beam angle of <10 degrees from 90 degrees significantly affected photodensity, and photodensity was significantly affected when the angle was varied <5 degrees from 60 degrees . When taken at an x-ray-beam angle of 60 degrees , the abaxial aspect of the radial facet of C3 had a consistently higher photodensity than the rest of C3 and C4. The photodensity of the third and fourth carpal bones was higher in exercised horses than in non-exercised horses. CONCLUSION: As variation in x-ray-beam angle significantly affected photodensity, RA using the tangential view is not considered clinically applicable for assessing BMD of C3 and the accuracy of subjective assessment of BMD of C3 using the tangential view in horses is questionable.  相似文献   

Musculoskeletal responses of 2-year-old Thoroughbred horses to early training. 3. In vivo ultrasonographic assessment of the cross-sectional area and echogenicity of the superficial digital flexor tendon     

Perkins NR  Rogers CW  Firth EC  Anderson BH 《New Zealand veterinary journal》2004,52(5):280-284

AIM: To determine if the superficial digital flexor tendon (SDFT) of young Thoroughbred horses changed in size and echogenicity in association with early race training. METHODS: Cross-sectional area (CSA) and echogenicity were determined ultrasonographically at five levels of the SDFT of the forelimbs of 2-year-old fillies (n=14), corresponding to 4, 8, 12, 16 and 20 cm distal to the accessory carpal bone (DACB). Measurements were made before and after a 13-week period in which a trained group of seven horses was compared with another group of seven untrained horses. RESULTS: Level below the accessory carpal bone had a significant effect on CSA and Level 8 was smaller than all other levels except Level 12, while Level 12 was smaller than Levels 4 and 20 but not different from Levels 8 and 16. There was a significant interaction between level and time due to effects observed at Level 8. The CSA at Level 8 measured pre-training was different from that of Levels 4 and 20 in both pre- and post-training groups (p<0.05), but when measured post-training was not different from any other measurement. There was also a significant interaction between treatment group and time. There was no difference between CSA for the untrained and trained groups at the pre-training observation (p=0.9), but post-training the CSA (pooled over all levels) in trained horses was significantly larger than that of the untrained horses both post-training (p=0.019) and pre-training (p=0.034), and was not different from the pre-training CSA recorded in the trained group (p=0.29). Treatment group had no effect on echogenicity (p=0.43), while echogenicity was less at the end of the trial in both trained and untrained horses (p<0.001). CONCLUSIONS: Early training for racing was associated with an increase in mean CSA of the SDFT. Other factors such as age and maturity may play a role in limiting this increase.  相似文献   

Changes in liver copper concentration of thoroughbred foals from birth to 160 days of age and the effect of prenatal copper supplementation of their dams     

Gee EK  Grace ND  Firth EC  Fennessy PF 《Australian veterinary journal》2000,78(5):347-353

OBJECTIVES: To monitor the change in liver copper concentration of Thoroughbred foals from birth to 160 days of age and to determine the effects of supplementation by two injections of copper edetate given to dams in late gestation on the liver copper concentration of their foals at birth. PROCEDURE: Ten mares pregnant to the same stallion were randomised into two groups on the basis of age, liver copper concentration and expected foaling date. The treatment group mares were given 100 mg and 250 mg copper edetate intramuscularly during the ninth and tenth months of gestation respectively. Foals had liver biopsies taken weekly in the first month of life, then monthly for four months. Foals were euthanased at 160 days of age; liver samples were taken and the copper concentrations were determined. RESULTS: Two distinct patterns of age dependent decline in liver copper concentration were evident. The mean (+/- SD) liver copper concentration of the foals was high at birth (374 +/- 130 mg/kg DM), and for seven it declined to adult values by 160 days of age (21 +/- 6 mg/kg DM). In three foals the decline was at a slower rate than in the other seven and at 160 days of age the mean concentration was 162 +/- 32 mg/kg DM. Repeated measures analysis showed significant differences between each biopsy (P < 0.01) and between 'normal' and 'accumulator' foals (P < 0.002). Copper injections given to mares in late pregnancy had no effect on the liver copper concentration of foals at birth. CONCLUSIONS: The significance of the two patterns of age dependant decline in liver copper concentration is unknown. Parenteral copper supplementation of the dam in late gestation had no effect on the liver copper concentration of the foal at birth.  相似文献   

Part 2: Treatment of Snail Bait Toxicity in Dogs: retrospective Study of 56 Cases     

Ava M. Firth DVM  MVS 《Journal of Veterinary Emergency and Critical Care》1992,2(1):31-36

A retrospective study was done of 26 cases of metaldehyde and 30 cases of methiocarb toxicity in dogs. Physical signs and treatment protocols are described for four stages of toxicity: asymptomatic exposure; initial toxicity; convulsions; and post-convulsions.
Diazepam-ketamine combination was used as the primaty anesthetic agent in all cases requiring general anesthesia, and proved effective. Metaldehyde cases required a significantly higher number of sedative and anesthetic doses (p < 0.001). Pentobarbitone was not used. There were no incidents of respiratory or cardiac arrest at the time of anesthesia induction.
Emetics, gastric Iavage, and rectal enemas were productive in removing the molluscicide from the gastrointestinal tract. Ninety percent of the methiocarb patients and 69% of the metaldehyde patients were fit for discharge in 12 hours or less, though there was a wide variation. Both metaldehyde and methiocarb groups had a high survival rate, 100% and 93%, respectively.  相似文献   

Derivation,safety and efficacy of a Marek's disease vaccine developed from an Australian isolate of very virulent Marek's disease virus   总被引：4，自引：0，他引：4  

Karpathy RC  Firth GA  Tannock GA 《Australian veterinary journal》2002,80(1-2):61-66

OBJECTIVE: To develop a serotype 1 Marek's disease (MD) vaccine from a very virulent MDV (vvMDV) pathotype and demonstrate safety and efficacy against early challenge with very virulent field strains in the presence of maternal antibody. STUDY DESIGN: Strain BH 16 was isolated and attenuated by serial cell culture passage. One of two cloned passages was selected for vaccine development following early laboratory-scale protection trials in commercial birds. Comparative protection trials were carded out on the BH 16 vaccine and on a CVI 988 Rispens vaccine using commercial and SPF chickens. Challenge viruses used were either a low passage strain BH 16 virus, the Woodlands No. 1 strain or MPF 57 strain of MDV. The BH 16 vaccine was back-passaged in SPF chickens six times and virus recovered from the final passage and the original vaccine virus were tested for safety. The immunosuppressive potential of the BH 16 and Rispens vaccines was also assessed in parallel. RESULTS: The BH 16 and Rispens vaccines induced comparable levels of protection when used as monovalent or multivalent vaccines, although protection achieved with the monovalent vaccines was lower. No gross tumour formation was evident in any birds receiving the BH 16 vaccine or bird-passaged virus, although microscopic lesions were present in 2/12 birds that received the bird-passaged virus. In tests for immunosuppression, there was no histological evidence of damage to either the bursa of Fabricius or the thymus. CONCLUSION: The BH 16 vaccine was shown to be safe and at least as protective as the Rispens vaccine against three highly virulent MD challenge viruses.  相似文献