Use of surface plasmon resonance (SPR) to study the dissociation and polysaccharide binding of casein micelles and caseins     

Thompson AK  Singh H  Dalgleish DG 《Journal of agricultural and food chemistry》2010,58(22):11962-11968

Tests were made to determine whether surface plasmon resonance (SPR) could be used as a technique to study the dissociation properties of bovine casein micelles or of sodium caseinate and the interactions between these protein particles and different polysaccharides. Surfaces of bound micelles or caseinate were made, and the changes in refractive index of these layers were used to define changes in the structures of the chemisorbed material. The technique appears to have some potential for studying details of the dissociation of casein micelles and of the binding of different polysaccharides to caseins.  相似文献   

Cartilage proteoglycans from normal and osteochondrotic porcine joints.          下载免费PDF全文

T Nakano  J R Thompson    F X Aherne 《Canadian journal of veterinary research》1985,49(2):219-226

Modern pigs grow fast but are highly susceptible to degenerative joint abnormalities, including osteochondrosis. Normal and osteochondrotic humeri and femurs were obtained from five normal and ten lame adolescent boars to study cartilage proteoglycans. Histological examination of joints indicated a locally-reduced intensity of proteoglycan staining by safranin-O in lesion areas of cartilage. Cartilage proteoglycans extracted with 4.0 M guanidinium chloride were studied using Sepharose 2B gel chromatography. The proteoglycans from severely osteochondrotic joints were less (P less than 0.05) aggregated and contained a greater (P less than 0.05) proportion of smaller monomers than those from normal joints. Loss or damage of core protein, including its hyaluronic acid-binding regions, may account for the greater proportion of small monomers. The results also indicated that the proportion of hyaluronic acid in the total glycosaminoglycan uronic acid fraction, estimated by Sephadex G-200 chromatography and cellulose acetate electrophoresis, was lower (P less than 0.05) for the extracted proteoglycans than for the residual or the whole cartilage proteoglycans in all joints studied.  相似文献   

First confirmed case of malignant catarrhal fever in a cow in Portugal.     

P P Cortez  P das Pereira  A Cortez  G Thompson 《The Veterinary record》2001,149(18):558-559

  相似文献   

Thymic hematoma in juvenile dogs associated with anticoagulant rodenticide toxicosis.     

Alan D Liggett  Larry J Thompson  Ken S Frazier  Eloise L Styer  Lowell T Sangster 《Journal of veterinary diagnostic investigation》2002,14(5):416-419

  相似文献   

Nasopharyngeal myxosarcoma in a Bengal tiger (Panthera tigris).     

Catherine M Shilton  Margret S Thompson  René Meisner  Brad Lock  William A Lindsay 《Journal of zoo and wildlife medicine》2002,33(4):371-377

An 18- to 25-yr-old intact female Bengal tiger (Panthera tigris) was diagnosed antemortem and postmortem with nasopharyngeal myxosarcoma metastatic to the lung, chronic lymphoplasmacytic otitis media, and lymphoplasmacytic and eosinophilic stomatitis. Myxosarcomas are rare in domestic animals and seldom metastasize; this tumor has not been previously reported in an exotic felid. Computed tomography of the skull was used during the diagnoses.  相似文献   

Antigen-induced production of interferon-gamma in samples of peripheral lymph nodes from sheep experimentally inoculated with Mycobacterium avium subsp. paratuberculosis.     

J M Gwozdz  K G Thompson 《Veterinary microbiology》2002,84(3):243-252

The production of interferon-gamma (IFN-gamma) in response to Johnin purified protein derivate was measured in samples of the prescapular lymph node (PLN) from 10 sheep, aged 2 years, and nine sheep, aged 1 year that had been inoculated orally with Mycobacterium avium subsp. paratuberculosis within their first month of life. Ten non-inoculated sheep, aged 1 year, constituted the negative control group. The results obtained in the PLN IFN-gamma assay were compared with those derived from serological tests: a complement fixation test (CFT), agar gel diffusion test (AGID) and enzyme-linked immunosorbent assay (ELISA), as well as an IFN-gamma test on samples of blood. Among the 19 inoculated sheep, 16 gave positive reactions in the PLN IFN-gamma assay on samples incubated overnight, and 18 tested positive when the assay was applied to PLN samples incubated for 48h. In comparison, three, four and seven inoculated sheep gave positive reactions in the ELISA, CFT and in the blood IFN-gamma assay on samples incubated overnight, respectively. The AGID and IFN-gamma assay on blood samples incubated for 48h detected eight inoculated animals. Twelve inoculated sheep, that tested positive in the PLN IFN-gamma assay were clinically normal, gave negative results in an IS900-based polymerase chain reaction (PCR) assay on samples of ileum and ileocaecal lymph node and had no histological evidence of paratuberculosis, but tested positive on more than two occasions in sequential serological testing before necropsy. None of the 10 non-inoculated sheep tested positive in the AGID, CFT, ELISA, blood IFN-gamma assay on samples incubated overnight and for 48h or the PLN IFN-gamma assay on samples incubated overnight, but one gave a positive result in the PLN IFN-gamma assay on samples stimulated for 48h. It is likely that the positive reactions obtained by the PLN IFN-gamma assay in the 12 inoculated sheep that tested negative in the PCR assay and histopathological examination represents immunological evidence of latent infection or previous exposure to M. paratuberculosis rather than active infection.  相似文献   

Yield and adaptation of hexaploid spring triticale   总被引：2，自引：0，他引：2  

P. N. Fox  B. Skovmand  B. K. Thompson  H. -J. Braun  R. Cormier 《Euphytica》1990,47(1):57-64

Summary Gains in yield and stability were analysed using grain yield data from the 8th to the 14th International Triticale Yield Nursery (ITYN) and differences in adaptation between complete and substituted triticales (X Triticosecale Wittmack) were examined. A trend of improving yield and adaptation was observed. As highly significant genotype × site interaction each year was not adequately described using regression analysis, yield stability was examined by ranking genotypes within sites and summarizing scores for individual genotypes across sites. Cluster analysis was used to study the relationships among sites and among genotypes. There was little evidence of sites grouping on a geographical basis. Grouping of genotypes showed a tendency for complete and substituted triticales to perform differently, suggesting that diversity for adaptation is maximized by utilizing both types in breeding.Progress for grain yield is compared with more spectacular gains in test weight and reasons for the different rates of improvement are postulated.Abbreviations CIMMYT International Maize & Wheat Improvement Centre - ITYN International Triticale Yield Nursery contribution from CIMMYT/Agriculture Canada (No. R-002)  相似文献   

Maize transformation utilizing silicon carbide whiskers: a review     

J. A. Thompson  P. R. Drayton  B. R. Frame  Kan Wang  J. M. Dunwell 《Euphytica》1995,85(1-3):75-80

  相似文献   

Relationship of morphological variables to rubber production in guayule     

D. A. Dierig  A. E. Thompson  D. T. Ray 《Euphytica》1989,44(3):259-264

Summary The objective of this study was to find measurable characters to aid the plant breeder in predicting both rubber percentage and rubber yield of guayule (Parthenium argentatum Gray). A field of 234 guayule breeding lines was harvested by clipping in February 1986. Forty-two of these lines were selected for further evaluation on the basis of superior regrowth, rubber content and yield. These 42 lines were reharvested in February 1987. Nineteen variables were evaluated including percent rubber and resin, rubber and resin yield (g/plant), plant height (cm), width (cm), and volume (m³), fresh and dry weight (kg/plant), percent dry weight, stem number, mean stem diameter (cm), total and mean stem circumference (cm), total and mean stem area (cm²), total stem circumference/total stem area (cm^-1), and regeneration at two dates. Dry weight (kg/plant) had the best correlation to rubber yield (r=0.85), and plant volume best predicted dry weight (r=0.83). These variables did not correlate as highly with percent rubber. The best statistical regression model for percent rubber included four variables, fresh and dry weight, percent dry weight, and plant volume. This model accounted for 51% of the variation. In comparison, the best regression of rubber yield had an R²=0.85 for plant height and width, volume, and dry weight.  相似文献   

Problems of official methods and new techniques for analysis of foods and feeds for vitamin A     

J N Thompson 《Journal of the Association of Official Analytical Chemists》1986,69(5):727-738

The official methods for measuring vitamin A and carotene in foods and feeds have evolved over several decades. New procedures are needed to permit the use of modern analytical techniques such as liquid chromatography (LC), and to expand the application of official methods to a wide variety of foods. Recent improvements in methodology are reviewed. Emphasis is often placed on the separations achieved by LC, but all factors that affect quantitation, such as sampling, preparation of standards, and protection of the vitamin against oxidation, must be considered in the development of new methods. Many approaches to analysis are possible because of the versatility of LC but not all of them can be used in official methods. Methods for milk, margarine, and feeds can be prepared now for collaborative assays. Although straightforward methods can be developed for the measurement of beta-carotene in certain foods, a full examination of provitamins will probably continue to require a flexible approach and special expertise.  相似文献