Technological Properties of Biodegradable Films Produced with Myofibrillar Proteins Extracted from Gilded cat-fish (Brachyplatystoma rousseauxii) Carcasses and Parings     

Jáira Thayse Souza Batista  Cleidiane Da Silva Araújo Matias  Maurício Madson Dos Santos Freitas  Gisélia De Sousa Nascimento  Lorena Limão Vieira  Lúcia De Fátima Henriques Lourenço 《Journal Of Aquatic Food Product Technology》2020,29(3):238-252

ABSTRACT

This paper aimed to investigate whether the type of fish residue used for extraction of myofibrillar proteins (MP) impacts the technological properties of biodegradable films. The MPs were extracted from two types of Gilded cat-fish (Brachyplatystoma rousseauxii) residues: (1) carcass with muscle attached from the production of mechanically separated meat (MSM) and (2) parings from fillet trimming (PAR). The percentage composition and color of the MPs from MSM (P-MSM) and parings (P-PAR) significantly differed, mainly regarding the ash and lipid contents. The presence of these non-protein compounds in P-MSM led to the formation of films with inferior technological and thermal properties, which indicates the type of residue used impacted the film properties.  相似文献   

Studies on antigenic and genomic properties of Brazilian rabies virus isolates   总被引：1，自引：0，他引：1  

Schaefer R  Batista HB  Franco AC  Rijsewijk FA  Roehe PM 《Veterinary microbiology》2005,107(3-4):161-170

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Effects of periodontal dressings on fibroblasts and gingival wound healing in dogs     

Petelin M  Pavlica Z  Batista U  Stiblar-Martincic D  Skaleric U 《Acta veterinaria Hungarica》2004,52(1):33-46

In the present study the effects of different commercially available periodontal dressings (Peripac, Barricaid, Fittydent, Reso-Pack and Myzotect-tincture) on fibroblast (V-79-379A) proliferation and survival were tested in vitro. Barricaid, Fittydent and Reso-Pack periodontal dressings have only small inhibitory effects on cell proliferation (83.3 +/- 9%, 71.6 +/- 8.7% and 87.3 +/- 4.5% of control after 48 h, respectively) in comparison with the great inhibitory effect of Myzotect-tincture (2.9 +/- 0.1%) and Peripac (33.7 +/- 11.4%) (p < 0.001). Barricaid was the only dressing where 41% of cells survived after exposure, while the other four dressings killed all the cells in 6 days. In addition, the healing of artificially created gingival wounds covered by Barricaid and Reso-Pack was followed for 7 days in 12 Beagle dogs. Histological evaluation of gingival tissue demonstrated that wounds covered by Reso-Pack showed the best epithelisation and vascularity and the least inflammatory reaction in first 4 days. Later the observed parameters were similar with those of wounds covered by Barricaid or without pack. The present results indicate that Peripac periodontal dressing and Myzotect-tincture showed the highest cytotoxicity to fibroblasts in vitro. From the histological observations in Beagle dogs Reso-Pack has been found to be the most suitable dressing, followed by Barricaid.  相似文献   

Nitrogen maintenance requirements and potential for nitrogen retention of pullets in growth phase     

Letícia Soares  Nilva Kazue Sakomura  Juliano Cesar de Paula Dorigam  Frank Liebert  Mariana Quintino do Nascimento  Joo Batista Kochenborger Fernandes 《Journal of animal physiology and animal nutrition》2019,103(4):1168-1173

Experiments were conducted to estimate daily N maintenance requirements (NMRs) and the genetic potential for daily N retention (NR_maxT) of pullets in growth phase. Three nitrogen balance trials were conducted, and a total of 48 Hy‐line W‐36 pullets were used in each trial in age periods (starter: 14–28, grower: 56–70 and developer: 98–112 days). The treatments consisted of six graded levels of nitrogen in the diets (L1 = 8, L2 = 16, L3 = 24, L4 = 32, L5 = 40 and L6 = 48 g N/kg of feed), formulated using the dilution technique. The regression analyses between nitrogen intake and nitrogen excretion were performed to fit the exponential function and to determine the NMR. The daily NMRs that were estimated at 294, 331 and 355 mg/BW_kg^0.67 for the initial, grower and developer periods, respectively, were applied for further calculation of NR_maxT as the threshold value of the function between N intake and daily N balance. The NR_maxT was estimated by a statistical procedure following several iteration steps by the Levenberg–Marquardt algorithm until the sum of the squares of the residual was minimized. The NR_maxT was estimated at 3,200, 2,633 and 1,826 mg/BW_kg^0.67 for starter, grower and developer periods respectively. The determined model parameters were the precondition for modelling of the amino acid requirement based on an exponential N‐utilization model and depended on performance and dietary amino acid efficiency. This procedure will be further developed and applied in the subsequent study.  相似文献   

Pregnancy rate after fixed‐time transfer of cryopreserved embryos collected by non‐surgical route in Lacaune sheep     

Lucas Machado Figueira  Nadja Gomes Alves  Ribrio Ivan Tavares Pereira Batista  Viviane Lopes Brair  Renato Ribeiro Lima  Maria Emilia Franco Oliveira  Jeferson Ferreira Fonseca  Joanna Maria Gonalves Souza‐Fabjan 《Reproduction in domestic animals》2019,54(11):1493-1496

This study investigated the feasibility of applying fixed‐time (cryopreserved) embryo transfer in ewes. Embryos (n = 106) were non‐surgically recovered from superovulated donors (n = 39) on day 6–7 after oestrus. Straws containing one or two embryos (morulae and/or blastocysts) subjected to either slow freezing (SF, n = 62) or vitrification (VT, n = 44) were randomly used within fixed‐time embryo transfer on Day 8.5. Recipient ewes were nulliparous (n = 58) bearing corpora lutea after synchronous oestrous induction protocol. The pregnancy rate was higher (p = .03) in SF (39.4%) than VT (16.9%) and survival rate tended (p = .08) to be higher in SF than in VT (25.8% vs. 15.9%). Lambing rates were similar (p = .13) between SF (20.9%) and VT (15.9%). Embryos recovered by non‐surgical route after cervical dilation treatment and later cryopreserved by either slow freezing or vitrification produced reasonable pregnancy rates after FTET.  相似文献   

Comparison of an alcohol-based hand rub and water-based chlorhexidine gluconate scrub technique for hand antisepsis prior to elective surgery in horses     

Eduardo Almeida da Silveira  Kirstin A. Bubeck  Edisleidy Rodriguez Batista  Perrine Piat  Sheila Laverty  Guy Beauchamp  Marie Archambault  Yvonne Elce 《The Canadian veterinary journal. La revue veterinaire canadienne》2016,57(2):164-168

This prospective clinical study evaluates the effectiveness of an alcohol-based hand rub (Avagard™) for pre-surgical hand antisepsis in an equine hospital and compares it with traditional scrubbing technique using 4% chlorhexidine gluconate sponges and water. Prior to elective surgery, 3 board-certified surgeons were randomly assigned to hand antisepsis with either technique. Culture samples of each hand were taken at 4 times: before and after neutral soap hand wash, after scrub or rubbing technique, and after surgery. There was no significant difference in mean bacterial colony forming units between scrub and rub techniques over the 3 time periods (P = 0.6), controlling for initial counts. One horse from the scrub group had a skin incision infection following stifle arthroscopy; this was resolved with medical treatment. The alcohol-based hand rub is equivalent in efficacy for pre-surgical hand antisepsis to traditional water-based scrubs in an equine hospital setting.  相似文献   

Effects of birth type and family on the variation of carcass and meat traits in Santa Ines sheep     

Adriana de Farias Jucá  Juliana Cantos Faveri  Geraldo Magalhães Melo Filho  Antônio de Lisboa Ribeiro Filho  Hymerson Costa Azevedo  Evandro Neves Muniz  Victor Breno Pedrosa  Luís Fernando Batista Pinto 《Tropical animal health and production》2016,48(2):435-443

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Peripheral blood mononuclear cell supernatants from asymptomatic dogs immunized and experimentally challenged with Leishmania chagasi can stimulate canine macrophages to reduce infection in vitro     

Rodrigues CA  Batista LF  Teixeira MC  Pereira AM  Santos PO  de Sá Oliveira GG  de Freitas LA  Veras PS 《Veterinary parasitology》2007,143(3-4):197-205

Leishmania chagasi is the causative agent of visceral leishmaniasis in both humans and dogs in the New World. The dog is the main domestic reservoir and its infection displays different clinical presentations, from asymptomatic to severe disease. Macrophages play an important role in the control of Leishmania infection. Although it is not an area of intense study, some data suggest a role for canine macrophages in parasite killing by a NO-dependent mechanism. It has been proposed that control of human disease could be possible with the development of an effective vaccine against canine visceral leishmaniasis. Development of a rapid in vitro test to predict animal responses to Leishmania infection or vaccination should be helpful. In this study, an in vitro model was established to test whether peripheral blood mononuclear cell (PBMC) supernatants from dogs immunized with promastigote lysates and infected with L. chagasi promastigotes could stimulate macrophages from healthy dogs in order to control parasite infection. PBMC from a majority of the immunized and experimentally infected dogs expressed IFN-gamma mRNA and secreted IFN-gamma when stimulated with soluble L. chagasi antigen (SLA) in vitro. Additionally, the supernatants from stimulated PBMC were able to reduce the percentage of infected donor macrophages. The results also indicate that parasite killing in this system is dependent on NO, since aminoguanidine (AMG) reversed this effect. This in vitro test appears to be useful for screening animal responses to parasite inoculation as well as studying the lymphocyte effector mechanisms involved in pathogen killing by canine macrophages.  相似文献   

Spatiotemporal dynamics of Citrus tristeza virus in Cuba     

L. Batista  K. Velázquez  I. Estévez  I. Peña  D. López  M. L. Reyes  D. Rodríguez  F. F. Laranjeira 《Plant pathology》2008,57(3):427-437

The spatiotemporal dissemination of Citrus tristeza virus (CTV) was evaluated by DASI-ELISA in orange and grapefruit fields of six citrus producing regions in Cuba, and aphid populations were evaluated in two selected areas. The aphid species found in these areas were Toxoptera citricida (the most efficient vector of CTV), T. aurantii and Aphis spiraecola . A logistic model was the most appropriate to explain the temporal increase in the proportion of CTV-infected plants for almost all the fields. Although nearly all areas showed an increase in this proportion, there were regions of low CTV spread, which was slower in grapefruit than in orange fields. 2DCORR analysis indicated spatial dependence among immediately adjacent trees, higher in the within-row direction than in the across-row direction. Aggregation was also detected by dispersion index and binary power law fitting within quadrats of all sizes. For most fields, autocorrelation analysis showed a significant edge effect and spatial dependence among infected plants of different sub-areas. Based on these results a new tristeza management strategy was proposed for each region of the country.  相似文献   

Dietary fatty acid enrichment during the spawning season increases egg viability and quality in Hippocampus hippocampus          下载免费PDF全文

M. Saavedra  H. Batista  P. Pousão‐Ferreira 《Aquaculture Nutrition》2016,22(2):343-351

Control of the reproductive cycle is essential to achieve a successful aquaculture industry. Seahorse reared in captivity often shows eggs of poor quality and low offspring survival due to unsuitable rearing and nutritional protocols. This study aimed to evaluate the effect of dietary enrichment, added exclusively during the spawning season, on the egg quality of Hippocampus hippocampus. The effect of enriching mysis throughout the spawning season was analysed in terms of female fecundity, egg viability, egg size and fatty acid profile. The results showed that fecundity was not affected by the enrichment of the diet, but egg viability was increased when females were fed the enriched mysis (84.9 ± 4.1% compared to 62.2 ± 9.4%). Moreover, the content of several PUFA (20:4n‐6, 18:2n‐6, 22:5n‐3, 22:5n‐6, sum of n‐6) decreased throughout the spawning season, especially in the last clutch, in eggs originated from females fed non‐enriched mysis. Eggs from females fed the enriched diet maintained the fatty acid profile throughout the spawning season. In conclusion, enrichment of the diet of H. hippocampus improved egg viability and avoided a decrease in the quality of eggs by maintaining PUFA content in the last clutches of the spawning season.  相似文献