Inhibition of photophosphorylation and electron transport chain in thylakoids by lasiodiplodin, a natural product from Botryosphaeria rhodina     

Veiga TA  Silva SC  Francisco AC  Filho ER  Vieira PC  Fernandes JB  Silva MF  Müller MW  Lotina-Hennsen B 《Journal of agricultural and food chemistry》2007,55(10):4217-4221

Four natural products were isolated from the fungus Botryosphaeria rhodina, and their effects on photosynthesis were tested. Only lasiodiplodin (1) inhibited ATP synthesis and electron flow from water to methylviologen; therefore, it acts as a Hill reaction inhibitor in freshly lysed spinach thylakoids. Photosystem I and II and partial reactions as well as ATPase were measured in the presence of 1. Three new different sites of 1 interaction and inhibition were found: one at CF1, the second in the water-splitting enzyme, and the third at the electron-transfer path between P680 and QA; these targets are different from that of the synthetic herbicides present. Electron transport chain inhibition by 1 was corroborated by fluorescence induction kinetics studies.  相似文献   

Genetic variability of banana with ornamental potential     

Everton Hilo de Souza  Maria Angélica Pereira de Carvalho Costa  Fernanda Vidigal Duarte Souza  Davi Silva Costa Junior  Edson Perito Amorim  Sebastião de Oliveira e Silva  Janay Almeida dos Santos-Serejo 《Euphytica》2012,184(3):355-367

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Comparison of two immunochromatographic assays and the indirect immunofluorscence antibody test for diagnosis of Trypanosoma cruzi infection in dogs in south central Louisiana     

Prixia D. Nieto  Roger Boughton  Patricia L. Dorn  Frank Steurer  Syamal Raychaudhuri  Javan Esfandiari  Edson Gonalves  James Diaz  John B. Malone 《Veterinary parasitology》2009,165(3-4):241-247

Two rapid tests evaluated in dogs considered to be of high risk of infection with the Chagas parasite Trypanosoma cruzi using two immunochromatographic assays: Trypanosoma Detect™ for canine, InBios, Seattle, WA and CHAGAS STAT-PAK™ assay, Chembio Diagnostic Systems, Medford, NY, in south central Louisiana. For this purpose a serological survey was carried out in a total of 122 dogs and a serum bank was created. These 122 animals were first tested by IFAT that was used as the standard test. From the serum bank 50 samples were tested using the two rapid Chagas assays and results compared to the standard test IFAT. The serological survey using IFAT showed a prevalence of T. cruzi infection in 22.1% of the tested dogs. In the immunochromatographic assays, 13 and 11 animals were positive on rapid assay: Trypanosoma Detect™ for canine, InBios and CHAGAS STAT-PAK™, Chembio Diagnostic Systems, respectively compared to 11 positive by IFAT. These two immunochromatographic tests have shown high susceptibility and specificity compared to our standard method IFAT. The rapid, easy and accurate screening assays used in conjunction with confirmatory tests, would be an excellent tool for veterinarians to diagnose T. cruzi infection. Early detection of T. cruzi infection may prevent complications through an effective treatment. Greater awareness by veterinarians of the risk, clinical findings, history along with diagnostic methods will contribute greatly to an understanding of the true prevalence of Chagas disease in dogs in Louisiana.  相似文献   

Characterisation and histopathological observations of a selected Brazilian precocious line of Eimeria acervulina     

Kawazoe U  Bordin EL  de Lima CA  Dias LA 《Veterinary parasitology》2005,131(1-2):5-14

Precocious lines of Eimeria acervulina "Cu" and "I" strains were obtained after 25 passages of oocysts in chickens that showed a shortening of the prepatent period for first oocyst output from 96 h to 81 and 82 h, respectively. Both precocious lines were evaluated for pathogenicity using as criteria weight gain, lesion score and total oocyst production. Infection of the "Cu" precocious line in chickens showed a high weight gain, low lesion score and low oocyst production, when compared to parent strain infected chickens. However, the results did not show a significant difference in relation to the criteria used above for the E. acervulina "I" precocious line when compared to its parent strain. This suggests a low degree of attenuation for the "I" strain but good attenuation for the precocious "Cu" line. The histopathological observations of chickens infected with the E. acervulina "Cu" parent strain and precocious line, comparing life cycle and intestinal lesions, showed: (1) parasite stages only in the border cells of infected chicken intestinal villi, for the precocious line; (2) parasite stages in the border cells of the intestinal villi and submucosa cells near the Lieberkühn glands of the intestine; and (3) high degree of inflammatory cells around the parasites in chickens infected with the parent strain. The "Cu" strain was also characterized for sensitivity against eight anticoccidial drugs. Sensitivity was observed for four anticoccidial drugs and partial resistance for four other drugs, although the strain had never had previous contact with anticoccidial drugs, suggesting the presence of a natural resistance factor. This Brazilian E. acervulina "Cu" precocious line showed attenuation for pathogenicity in chickens, suggesting that it could be a suitable strain for use as a live vaccine in Brazil.  相似文献   

Multiplex Nested Reverse Transcription-Polymerase Chain Reaction in a Single Tube for Sensitive and Simultaneous Detection of Four RNA Viruses and Pseudomonas savastanoi pv. savastanoi in Olive Trees     

Bertolini E  Olmos A  López MM  Cambra M 《Phytopathology》2003,93(3):286-292

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The Current State of Solar Modeling     

J Christensen-Dalsgaard  W Dappen  SV Ajukov  ER Anderson  HM Antia  S Basu  VA Baturin  G Berthomieu  B Chaboyer  SM Chitre  AN Cox  P Demarque  J Donatowicz  WA Dziembowski  M Gabriel  DO Gough  DB Guenther  JA Guzik  JW Harvey  F Hill  G Houdek  CA Iglesias  AG Kosovichev  JW Leibacher  P Morel  CR Proffitt  J Provost  J Reiter  EJ Rhodes  FJ Rogers  IW Roxburgh  MJ Thompson  RK Ulrich 《Science (New York, N.Y.)》1996,272(5266):1286-1292

Data from the Global Oscillation Network Group (GONG) project and other helioseismic experiments provide a test for models of stellar interiors and for the thermodynamic and radiative properties, on which the models depend, of matter under the extreme conditions found in the sun. Current models are in agreement with the helioseismic inferences, which suggests, for example, that the disagreement between the predicted and observed fluxes of neutrinos from the sun is not caused by errors in the models. However, the GONG data reveal subtle errors in the models, such as an excess in sound speed just beneath the convection zone. These discrepancies indicate effects that have so far not been correctly accounted for; for example, it is plausible that the sound-speed differences reflect weak mixing in stellar interiors, of potential importance to the overall evolution of stars and ultimately to estimates of the age of the galaxy based on stellar evolution calculations.  相似文献   

Experimental and serologic observations on avian pneumovirus (APV/turkey/Colorado/97) infection in turkeys   总被引：1，自引：0，他引：1  

Panigrahy B  Senne DA  Pedersen JC  Gidlewski T  Edson RK 《Avian diseases》2000,44(1):17-22

An avian pneumovirus (APV) was isolated from commercial turkeys in Colorado (APV/Colorado) showing clinical signs of a respiratory disease. The results of virus neutralization and indirect fluorescent antibody tests showed that the APV/Colorado was partially related to APV subgroup A but was unrelated to APV subgroup B. Turkeys experimentally inoculated with the APV/Colorado were observed for signs, lesions, seroconversion, and virus shedding. Thirty-six 7-wk-old turkeys were distributed into three groups. Eighteen turkeys were inoculated oculonasally with APV/Colorado, six were placed in contact at 1 day postinoculation (DPI), and 12 served as noninoculated controls. Tracheal swabs and blood samples were collected at 3, 5, 7, 10, 14, and 21 DPI. Tissues were collected from three inoculated and two control turkeys on aforementioned days for pathologic examination and APV isolation. Inoculated turkeys developed respiratory disease, yielded APV at 3, 5, and 7 DPI, and seroconverted at 10 DPI. Contact turkeys yielded APV at 7 and 10 DPI. No gross lesions were observed in the turbinates, infraorbital sinuses, and trachea. However, microscopic examination revealed acute rhinitis, sinusitis, and tracheitis manifested by congestion, edema, lymphocytic and heterophilic infiltration, and loss of ciliated epithelia. The inflammatory lesions were seen at 3 DPI and became extensive at 5 and 7 DPI. Active regenerative changes in the epithelia were seen at 10 and 14 DPI. Serologic survey for the presence of antibodies in commercial turkeys (24,504 sera from 18 states) and chickens (3,517 sera from 12 states) to APV/Colorado showed seropositive turkeys in Minnesota, North Dakota, and South Dakota and no seropositive chickens. This report is the first on the isolation of an APV and APV infection in the United States.  相似文献   

Characterization of antibiosis to the silverleaf whitefly Bemisia tabaci biotype B (Hemiptera: Aleyrodidae) in cowpea entries     

Patrícia L. Cruz  Edson L. L. Baldin  Maria de Jesus P. de Castro 《Journal of pest science》2014,87(4):639-645

The silverleaf whitefly, Bemisia tabaci (Gennadius) biotype B (Hemiptera: Aleyrodidae), is considered one of the most important pests of cowpea (Vigna unguiculata L. Walp.), limiting the productivity of this crop worldwide. Chemical control is still the main strategy for the management of populations of this insect. However, due to the harmful effects of pesticides on the environment and to humans, less injurious alternatives have been investigated. Along this line, the use of resistant genotypes can be a valuable tool for the control of the silverleaf whitefly. In this paper, we investigate some biological aspects of B. tabaci biotype B confined on 14 genotypes of cowpea. We evaluated the incubation period, egg viability, duration of nymphal stages, total duration of the juvenile phase, instar mortality and total mortality of the immature stage. The genotype MNC 99-541 F21 exhibited antibiosis against the whitefly, prolonging the lifecycle of the insect. The genotypes Canapu, BRS-Urubuquara and TE97-304 G-4 also exhibited antibiosis, causing high nymphal mortality. These results may help in breeding programmes to develop cowpea lines with resistance to B. tabaci biotype B.  相似文献   

Antiproliferative effect of Canavalia brasiliensis lectin on B16F10 cells     

Flávio de Oliveira Silva  Priscila das Neves Santos  Evellyne de Oliveira Figueirôa  Cristiane Moutinho Lagos de Melo  Juliana Kelle de Andrade Lemoine Neves  Francisco Vassiliepe Sousa Arruda  João Batista Cajazeiras  Kyria Santiago do Nascimento  Edson Holanda Teixeira  Benildo Sousa Cavada  Ana Lúcia Figueiredo Porto  Valéria Rêgo Alves Pereira 《Research in veterinary science》2014

Lectins are proteins or glycoproteins with the ability to link to carbohydrates at the cell surface in a specific and reversible manner. Studies have showed that lectins are demonstrate immunomodulatory and antitumor agents. This study aims to evaluate the effect of lectin extracted from the seeds of Canavalia brasiliensis (ConBr) on murine melanoma B16F10 cells by analyzing cell viability, apoptosis index, cell migration, production of cytokines and nitric oxide (NO). Results showed that ConBr was able to reduce cell viability and thwart apoptosis, which could be observed by decrease in cell migration. ConBr also induced NO and IL-12 synthesis. Altogether, these data demonstrate the potential of ConBr as a therapeutic agent for melanoma.  相似文献   

Heart structure and coronary blood supply of the Giant anteater (Myrmecophaga tridactyla)     

Nathany Ribeiro Lima dos Santos  Edson José Benetti  Kleber Mirallia de Oliveira  Max Vinicius Malmann Medeiros  Karina Simões 《Anatomia, histologia, embryologia》2021,50(1):15-22

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