Ovsynch Plus protocol improves ovarian response in anovular Murrah buffaloes in low‐breeding season          下载免费PDF全文

RK Sharma  SK Phulia  A Jerome  I Singh 《Reproduction in domestic animals》2017,52(6):1030-1035

The objective of the study was to evaluate the efficacy of ovarian response and pregnancy rate in anovular buffaloes following Ovsynch and Ovsynch Plus protocols. Buffaloes (n = 55) were divided into two groups: Ovsynch group (n = 26): GnRH (10 μg, GnRH1) on Day 0, PGF₂α (25 mg) on Day 7, GnRH (10 μg, GnRH2) on Day 9; Ovsynch Plus group (n = 29): 500 IU equine chorionic gonadotropin (eCG) 72 hr (day ?3) prior to Ovsynch protocol, followed by fixed timed artificial insemination (FTAI) 6 and 24 hr after GnRH2 injection in bot groups. Transrectal ultrasonography was performed daily, that is, from day 0 and ?3 in Ovsynch and Ovsynch Plus group, respectively for ovarian response and pregnancy diagnosis at day 30 post‐insemination. In Ovsynch Plus group, administration of eCG prior to GnRH1 increased (p < .001) the diameter (mm) of dominant follicle (DF) from 10.15 ± 0.26 to 12.23 ± 0.34 within 72 hr of treatment resulting higher ovulatory response to GnRH1. Ovulation after GnRH1 was higher (p < .01) in Ovsynch Plus group (96.6%) than Ovsynch group (61.5%). However, ovulation rate to GnRH2 was similar (p > .05) between groups (Ovsynch group: 76.9% vs. Ovsynch Plus group: 70.0%). Mean DF diameter (mm) that ovulated to both GnRHs was higher (p < .01) than non‐ovulated counterparts in both groups (Ovsynch group: 10.80 ± 0.27 vs. 8.47 ± 0.53; Ovsynch Plus group: 11.99 ± 0.24 vs. 9.5 ± 0.63). Pregnancy was established in buffaloes which responded to both GnRHs, irrespective of groups, being higher (p = .52) in Ovsynch Plus group (34.5%) than Ovsynch group (23.1%), though non‐significant. In summary, this study showed that eCG inclusion prior to Ovsynch regimen improves ovulatory response in anovular buffaloes during low‐breeding season.  相似文献   

Intraguild interactions and behavior of Spodoptera frugiperda and Helicoverpa spp. on maize          下载免费PDF全文

José PF Bentivenha  Débora G Montezano  Thomas E Hunt  Edson LL Baldin  Julie A Peterson  Vinícius S Victor  Luiz ER Pannuti  Ana M Vélez  Silvana V Paula‐Moraes 《Pest management science》2017,73(11):2244-2251

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Bioremoval of Surfactant from Laundry Wastewater in Optimized Condition by Anoxic Reactors     

Marcus Vinicius Freire Andrade  Isabel Kimiko Sakamoto  Aline Gomes de Oliveira Paranhos  Edson Luiz Silva  Maria Bernadete Amâncio Varesche 《Water, air, and soil pollution》2017,228(4):165

Effects of ethanol and nitrate on linear alkyl benzene sulfonate (LAS) degradation were investigated using central composite design. At experimental design, removal of 99.9% was observed in batch reactors (1 L) with 9.8 to 41.2 mg L^?1 of LAS. The batch reactors were kept under agitation at 120 rpm and 30 °C. Ethanol (co-substrate) and nitrate (electron acceptor) were statistically significant factors (p?<?0.05) in surfactant removal. Optimal values were 97.5 and 88 mg L^?1 for ethanol and nitrate, respectively. LAS removal was kinetically investigated by varying surfactant concentration while using optimal values. Batch I (27 mg L^?1 LAS) exhibited greater degradation rate (K^LAS) (0.054 h^?1) in the presence of ethanol and nitrate. Nonetheless, in Batch II (60 mg L^?1 LAS), the K^LAS values decreased in those reactors probably due to inhibition by excess substrate for same concentrations of nitrate and ethanol added in reactors. As LAS concentration increased, the dominance of bacterial populations also increased, whereas diversity index decreased from 2.8 (inoculum) to 2.4 and 2.5 for reactors with both added nitrate and ethanol and those with only added ethanol, respectively. Probably, a selection of microbial populations occurred in relation to LAS concentration. The nitrate and ethanol, at able concentration, made it possible the induction of denitrifying microrganisms foward to LAS removal.  相似文献   

A Model for comparing reduced impact logging with conventional logging for an Eastern Amazonian Forest     

Alexander J. Macpherson  Mark D. Schulze  Douglas R. Carter  Edson Vidal 《Forest Ecology and Management》2010

Using data from a logging experiment in the eastern Brazilian Amazon region, we develop a matrix growth and yield model that captures the dynamic effects of harvest system choice on forest structure and composition. Multinomial logistic regression is used to estimate the growth transition parameters for a 10-year time step, while a Poisson regression model is used to estimate recruitment parameters. The model is designed to be easily integrated with an economic model of decisionmaking to perform tropical forest policy analysis. The model is used to compare the long-run structure and composition of a stand arising from the choice of implementing either conventional logging techniques or more carefully planned and executed reduced-impact logging (RIL) techniques, contrasted against a baseline projection of an unlogged forest. Results from “log and leave” scenarios show that a stand logged according to Brazilian management requirements will require well over 120 years to recover its initial commercial volume, regardless of logging technique employed. Implementing RIL, however, accelerates this recovery. Scenarios imposing a 40-year cutting cycle raise the possibility of sustainable harvest volumes, although at significantly lower levels than is implied by current regulations. Meeting current Brazilian forest policy goals may require an increase in the planned total area of permanent production forest or the widespread adoption of silvicultural practices that increase stand recovery and volume accumulation rates after RIL harvests.  相似文献   

Characterization of antibiosis and antixenosis to the whitefly silverleaf <Emphasis Type="Italic">Bemisia tabaci</Emphasis> B biotype (Hemiptera: Aleyrodidae) in several squash varieties     

Edson Luiz Lopes Baldin  Ronaldo Antonio Beneduzzi 《Journal of pest science》2010,83(3):223-229

The objective of this study was to investigate the potential resistance mechanisms to Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) B biotype, in eight Cucurbita pepo varieties. Attractivity, oviposition preference, and nymphal development were evaluated under greenhouse and laboratory conditions. Variety Bianca was the least attractive to adult whiteflies in multi-choice tests. The whiteflies showed oviposition nonpreference (antixenosis) for Novita Plus, Bianca and Sandy in multi-choice test, and similar results were observed in no-choice tests with varieties Bianca, Novita, Caserta Cac and Sandy. Sandy expressed high antibiosis levels against whitefly nymphs, leading to 63.9% mortality. The high level of resistance in variety Sandy can serve as base for genetic improvement of C. pepo focusing on the development of resistant varieties to B. tabaci B biotype.  相似文献   

Bacterial pigment for Nile tilapia feeding     

Thiago Luís Magnani Grassi  Edson Francisco do Espírito Santo  Marcelo Tacconi de Siqueira Marcos  Jefferson Felipe Cavazzana  Dayse Lícia Oliveira  Iderlipes Luiz Carvalho Bossolani  Elisa Helena Giglio Ponsano 《Aquaculture International》2016,24(2):647-660

The aim of this study was to evaluate the effects of different carotenoids sources in tilapia fish diets on the animals’ performance and fillets characteristics. Nine hundred and sixty tilapias, Oreochromis niloticus, averaging 15 g were distributed into 24 tanks to receive one of the six treatments (four repetitions) for 80 days: basal diet with no pigment (control group), basal diet with 350 mg/kg astaxanthin 10 % and basal diets added of four different concentrations of Rubrivivax gelatinosus biomass (175, 350, 700 and 1400 mg/kg). Variables analyzed included feed consumption, weight gain, feed conversion and specific growth rate for the animals and pH, proximate composition, carotenoids, polyunsaturated fatty acids and color for the fillets. Productive parameters did not differ statistically. Moisture content was lower on the fillets of treatments with pigments. The protein contents on the fillets of diets supplemented with the bacterial biomass were higher than in control group, while pH, minerals and lipids did not vary among treatments. Lightness and yellowness did not differ among the treatments, but redness and carotenoids contents were higher for all the groups that received the pigments than for the control group. The ratio of polyunsaturated fatty acids n-6/n-3 was improved with the dietary biomass. So, it was concluded that the use of the pigmenting ingredients did not alter productive parameters but increased redness and carotenoids contents in the fillets. Moreover, the use of R. gelatinosus biomass also increased the protein contents and improved the fatty acids profile in the fillets.  相似文献   

Investigation of insecticidal activity of rye α-amylase inhibitor gene expressed in transgenic tobacco (Nicotiana tabacum) toward cotton boll weevil (Anthonomus grandis)     

Simoni Campos Dias  Maria Cristina Mattar da Silva  Edson Luis Zangrando Figueira  Osmundo Brilhante de Oliveira-Neto  Loaiane Alves de Lima  Maria Fátima Grossi-de-Sa 《Pesticide biochemistry and physiology》2010,98(1):39-4821

Innumerable proteinaceous α-amylase inhibitors have been isolated and identified from different plant species. Among them, an α-amylase inhibitor gene with bioinsecticidal potential toward Anthonomus grandis (cotton boll weevil) was previously identified in rye seeds (Secale cereale). This cereal inhibitor was expressed in tobacco plants (Nicotiana tabacum) under control of phytohemaglutinin promoter by using Agrobacterium tumefasciens - mediated transformation. Presence of αBIII-rye gene and further protein expression were confirmed by PCR and Western blot analysis, respectively. Immunological assays indicated that the recombinant inhibitor was expressed in concentration range from 0.1% to 0.28% (w:w) of the total protein in tobacco seeds of R₀ plants. From 14 independent transformants, five plants with expression levels between 0.20% and 0.28% in seeds were in vitro assayed against A. grandis amylolytic enzymes causing clear inhibition. Moreover, bioassays using transgenic seed flour mixture for artificial diet produced 74% mortality in A. grandis first larval instar. These data suggest that rye inhibitor could be a promising biotechnological tool for produce transgenic cotton plants with an increased resistance to cotton boll weevil. Moreover, αBIII-rye gene should be considered a potential compound for a pyramiding strategy aiming to delay insect-resistance.  相似文献   

Evaluating ipê (Tabebuia, Bignoniaceae) logging in Amazonia: Sustainable management or catalyst for forest degradation?     

Mark Schulze  James Grogan  Chris Uhl  Edson Vidal 《Biological conservation》2008,141(8):2071-2085

Prized for their dense, rot-resistant wood, Tabebuia impetiginosa and T. serratifolia (vernacular name = ipê) are among the most valuable Amazonian timbers. We analyzed the geographical extent, spread and trajectory of ipê logging in Brazilian Amazonia, and evaluated harvest pressure on this forest resource. We also examine Tabebuia population response to reduced-impact logging, a more ecologically benign alternative to destructive conventional harvest practices in Amazonia. Based on eight years of population monitoring at multiple sites in the eastern Brazilian Amazon, we project second harvest ipê yields in forests logged using RIL under legally allowable (90% of commercial stems) and reduced (70%) harvest intensities.In recent years ipê harvests have declined or ceased in the majority of old logging frontiers in eastern Amazonia while spreading to new logging frontiers in central and southwestern Amazonia. With current timber market prices, transportation infrastructure and harvesting costs, logging of ipê would be profitable in an estimated 63% of the Brazilian Amazon; in the more remote logging frontiers only logging of ipê and a few other high-value timbers is currently profitable. All populations of T. impetiginosa and T. serratifolia in northeastern forests showed drastic population declines over multiple RIL harvests in simulations, with no indication of population recovery over the long term. We conclude from study of Tabebuia populations in eastern Amazonia and modeling of response to logging that these two species are endangered by logging activity and merit additional protection under forest legislation.  相似文献   

Relationships among bovine male and female reproductive traits     

VEA PERRY  RK MUNRO†  PJ CHENOWETH  DAV BODERO  TB POST† 《Australian veterinary journal》1990,67(1):4-5

No significant relationship (p greater than 0.05) was found between age at puberty in heifers and the age and scrotal circumference at puberty in related bulls. There was a significant effect (p less than 0.01) of genotype and sire on age at puberty of heifers and a significant effect (p less than 0.05) of genotype on weight at puberty in heifers. There was a significant effect of genotype on age (p less than 0.01) and weight (p less than 0.05) at puberty of bulls. A significant difference (p less than 0.05) in age at puberty of bulls was found between the 2 methods of assessing puberty. It is possible that the assessment of puberty of heifers at 2-month intervals may not have been precise enough to detect such a relationship and/or that the variation in genotypes and ages in this study were too great to establish such a relationship.  相似文献   

An evaluation of calf castration by intra-testicular injection of a lactic acid solution   总被引：1，自引：0，他引：1  

G. FORDYCE  NJ BEAMAN  AR LAING  PB HODGE†  C. CAMPERO†  RK SHEPHERD¶ 《Australian veterinary journal》1989,66(9):272-276

This experiment evaluated intra-testicular injection of a sclerosing drug, lactic acid, for castration of bulls. Its use was compared in 58 Brahman cross calves (50 to 128kg) with the general practice of open surgical castration. Chemical castration appeared to be more painful than surgical castration, though post-operative swelling and pain appeared similar for both methods. Chemical castration took 3 times longer than surgical castration (58 sec v 20 sec; P less than 0.01). Scrotal necrosis occurred in 25% of chemically-castrated calves and appeared due to drug leakage from the testes under the high pressure of injection. Healing time for chemical castrates was approximately twice that for surgical castrates. Five chemically-castrated calves (18%) retained one testis. Though all 5 were rendered sterile, each maintained androgenesis. This led to secondary male behaviour which caused management problems. Castration method did not influence post-operative growth. It is concluded that lactic acid administration is not a suitable alternative to the open surgical technique for castration of Brahman cross calves.  相似文献