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91.
92.
Tall perennial grass species can be utilised as bioenergy feedstocks, but some are considered invasive species. Using biomass from such species as feedstocks for anaerobic digestion (AD) may introduce the risk of disseminating viable seeds onto agricultural lands during digestate application. To evaluate this risk, we investigated the survival rates of perennial grass seeds obtained from biomass species during AD. After removal from the digester, seeds were germinated and stained with tetrazolium chloride to determine viability. During three experimental runs, batches of 100 seeds from four species were exposed to 0, 2, 6, 12, 24, 48, 72 and 168 h of mesophilic (38°C) AD within a commercial‐scale digester. Seed viability of Phalaris arundinacea, Phragmites australis, Panicum virgatum and Solanum lycopersicum was reduced by 95% (LT95) after 29, 52, 98 and 105 h of AD respectively. Commercial digesters that utilise perennial grasses as a feedstock typically have retention times ranging from 240 to 1480 h, which greatly exceeds the LT95 values found in this study. Anaerobic digestion resulted in the rapid death of seeds in all species tested, suggesting unwanted dissemination of perennial grass species via digestate application to agricultural land is unlikely.  相似文献   
93.
94.
Urban sediment particle size and pollutants in Southern Brazil   总被引:1,自引:1,他引:0  

Background, aim and scope

Studies of particulate-associated pollutants, or PAPs, in urban areas have become necessary due to their potentially deleterious effects on the environment. However, it is not just the sediments themselves which are problematic but also their particle size composition, which has a great influence on their capacity to adsorb and transport pollutants. This paper presents the particle size distributions and concentrations of five metals (Cr, Cu, Ni, Pb and Zn) of urban sediments collected from paved streets and gully pots from 20 cities in southern Brazil. The cities have different characteristics and hence sources of PAPs associated with differing geologies, soil types and type of urbanisation. Studies of this nature enable elucidation of the relationship between diffuse sources such as streets and gully pots and the likelihood of PAPs to subsequently pollute the urban aquatic environment.

Materials and methods

Sediment samples were taken at random from paved streets and gully pots in 20 cities in Rio Grande do Sul state, southern Brazil by means of a portable vacuum cleaner to avoid loss of finer particles. The particle sizes of the samples were measured using a Cilas® 1180 laser particle analyzer, and the concentrations of five metals (Cr, Cu, Ni, Pb and Zn) were determined by wet acid digestion (HCl–HF–HClO4–HNO3) followed by inductively coupled plasma atomic emission spectroscopy on the <63-µm fraction.

Results

It was found that in comparison to sediments collected from the streets, gully pot sediments were more heterogeneous in terms of particle size and also that sediment samples from the gully pots were predominantly coarser than those originating on the streets. From the gully pot results, analysis of the modal particle diameter enabled the cities to be divided into three categories. The concentrations of metals in the street sediments were similar across all 20 cities, with all concentrations above background values.

Discussion

The fact that concentrations of metals in the street sediments were above statutory guideline values and that the coarser material was deposited in the gully pots suggests that the finer, more polluted sediment is not retained in the gully pots but is transported to the nearest local receiving watercourse. This finding has implications for management strategies for reducing pollution in urban environments.

Conclusions

High concentrations of Cr, Cu, Ni, Pb and Zn in the <63-µm fraction of street sediments, in combination with coarse material retained in the gully pots, indicate that metals could be transferred quite rapidly from the diffuse source of pollutants, on impermeable street surfaces, to receiving watercourses.

Recommendations and perspectives

Studies of urban sediment particle size and geochemistry enable predictions to be made of sediment behaviour in urban environments. This will inform management strategies such as the possibility of including sustainable urban drainage systems in future management plans, in which it is useful to know how efficient the drainage system is from the point of view of sediment deposition in the urban aquatic environment and the potential for pollution of receiving waters.  相似文献   
95.
The fall armyworm, Spodoptera frugiperda (J. E. Smith) and the corn earworm, Helicoverpa zea (Boddie) are among the main pests of maize. Both species exhibit cannibalistic behavior and quite often share the same feeding guild in maize (maize ears), which can result in several interspecific and intraspecific interactions. Paired interaction scenarios of intraspecific and interspecific larvae were assessed in arenas in the presence and absence of food to characterize movements resulting from interactions of these insects. There was a difference in the frequency of behavioral movements in all the interactions, except for S. frugiperda in the presence of food. Head touching and recoiling were the predominant movements in most of the interaction scenarios. Spodoptera frugiperda exhibited a predominance of defensive movements when competing against H. zea in the same instars. Cannibalism and predation occurred frequently in interactions involving 6th instar of H. zea against opponents in 4th instar. Larvae of H. zea show a higher aggressive movement than S. frugiperda. The larvae of S. frugiperda take advantage during the interactions, although they present more defensive movements compared to H. zea. This study provides relevant information regarding the interaction of these species and intraguild interaction, which might influence the population dynamics and the competitive displacement of pest species that share the same ecological niche.  相似文献   
96.
Hendra virus (HeV) causes potentially fatal respiratory and/or neurological disease in both horses and humans. Although Australian flying‐foxes of the genus Pteropus have been identified as reservoir hosts, the precise mechanism of HeV transmission has yet to be elucidated. To date, there has been limited investigation into the role of haematophagous insects as vectors of HeV. This mode of transmission is particularly relevant because Australian flying‐foxes host the bat‐specific blood‐feeding ectoparasites of the genus Cyclopodia (Diptera: Nycteribiidae), also known as bat flies. Using molecular detection methods, we screened for HeV RNA in 183 bat flies collected from flying‐foxes inhabiting a roost in Boonah, Queensland, Australia. It was subsequently demonstrated that during the study period, Pteropus alecto in this roost had a HeV RNA prevalence between 2 and 15% (95% CI [1, 6] to [8, 26], respectively). We found no evidence of HeV in any bat flies tested, including 10 bat flies collected from P. alecto in which we detected HeV RNA. Our negative findings are consistent with previous findings and provide additional evidence that bat flies do not play a primary role in HeV transmission.  相似文献   
97.
Antioxidants are known to prevent the reactive oxygen species (ROS)‐mediated peroxidative damage to the membrane lipids during hypothermic storage of mammalian spermatozoa. We hypothesized here that ROS also affect the lipid–protein interactions, thereby diminishing the membrane's integrity and proteins' anchorage to the bilayer. Antioxidants prevent these damages by scavenging the ROS. Ejaculates from Patanwadi rams were pooled after subjective evaluation and centrifuged using Percoll®. Sperm pellet was resuspended in soya lecithin–Tris–fructose diluent (400 × 106 cells/ml) containing either antioxidants (100 IU/ml catalase + 10 mM reduced glutathione) or no antioxidant. Aliquots were chilled to 5°C in a cabinet and stored in a refrigerator at 3–5°C for 72 hr. Sperm motility, viability, lipid peroxidation (LPO) and hypo‐osmotic swelling test (HOST) were performed at 0, 24, 48 and 72 hr. Sperm proteins extracted with 0.5% Triton X‐100 were resolved by SDS‐PAGE and quantified using Quantity One software (Bio‐Rad, USA). The rapid motility, linearity and straight‐line velocity (VSL) were found significantly (p < .05) higher in the antioxidant‐treated group compared to the control at 48 hr of storage. Sperm viability was found comparable between the groups. Higher HOST response and lower LPO were found in the antioxidant‐treated sample compared to the control both at 48 and at 72 hr. Overall, the proteins P1 (106.09 kDa), P2 (87.00 kDa) and P4 (51.14 kDa) were lower (p < .05) in the sperm extract of antioxidant‐treated group compared to the control. The content of P4 (51.14 kDa) in sperm extract was found to increase (p < .05) earlier (48 vs. 72 hr) in the control group compared to the antioxidant‐treated group. Altogether, the results suggested that antioxidants reduced LPO in spermatozoa, resulting in higher sperm motility, plasma membrane integrity and protection of proteins' anchorage to the plasma membrane at 48 and 72 hr of storage.  相似文献   
98.
This study compared artificial insemination pregnancy rate (AI‐PR) between 14‐day CIDR‐GnRH‐PGF2α‐GnRH and CIDR‐PGF2α‐GnRH synchronization protocol with two fixed AI times (56 or 72 hr after PGF2α). On day 0, heifers (= 1311) from nine locations assigned body condition score (BCS: 1, emaciated; 9, obese), reproductive tract score (RTS: 1, immature, acyclic; 5, mature, cyclic) and temperament score (0, calm; and 1, excited) and fitted with a controlled internal drug release (CIDR, 1.38 g of progesterone) insert for 14 days. Within herd, heifers were randomly assigned either to no‐GnRH group (= 635) or to GnRH group (= 676), and heifers in GnRH group received 100 μg of GnRH (gonadorelin hydrochloride, IM) on day 23. All heifers received 25 mg of PGF2α (dinoprost, IM) on day 30 and oestrous detection aids at the same time. Heifers were observed for oestrus thrice daily until AI. Within GnRH groups, heifers were randomly assigned to either AI‐56 or AI‐72 groups. Heifers in AI‐56 group (= 667) were inseminated at 56 hr (day 32 PM), and heifers in AI‐72 group (= 644) were inseminated at 72 hr (day 33 AM) after PGF2α administration. All heifers were given 100 μg of GnRH concurrently at the time AI. Controlling for BCS (< .05), RTS (< .05), oestrous expression (< .001), temperament (< .001) and GnRH treatment by time of insemination (< .001), the AI‐PR differed between GnRH treatment [GnRH (Yes – 60.9% (412/676) vs. No – 55.1% (350/635); < .05)] and insemination time [AI‐56 – 54.6% (364/667) vs. AI‐72 – 61.8% (398/644); (< .01)] groups. The GnRH treatment by AI time interaction influenced AI‐PR (GnRH56 – 61.0% (208/341); GnRH72 – 60.9% (204/335); No‐GnRH56 – 47.9% (156/326); No‐GnRH72 – 62.8% (194/309); < .001). In conclusion, 14‐day CIDR synchronization protocol for FTAI required inclusion of GnRH on day 23 if inseminations were to be performed at 56 hr after PGF2α in order to achieve greater AI‐PR.  相似文献   
99.
Myxobolus porofilus sp. n. is described infecting the visceral cavity of Prochilodus lineatus (Valenciennes, 1836) cultivated in S?o Paulo State, Brazil. The plasmodial form of the parasite is 3-5 mm in length and appeared compressed between the wall of the visceral cavity and the pyloric caecum, reposing on this organ. The spores are small (length 5.7 +/- 0.3 microm, width 4.8 +/- 0.2 microm; mean +/- SD) and round to elliptical in frontal view. The valve surfaces are smooth and have sutural folds. The polar capsules are ovoid, small (length 1.6 +/- 0.1 microm, width 1.1 +/- 0.1 microm) and equal in size. The polar filaments have three turns aligned perpendicularly to the longitudinal axis of the capsule. A conspicuous polar filament pore is arranged at the anterior end of the spore. The only reaction observed upon histological analysis was the presence of a capsule of connective tissue surrounding the plasmodia. This is the first report of a myxosporean parasite in the Prochilodontidae.  相似文献   
100.
The spatial distribution of adult individuals (≥15 cm DBH) was investigated in a gallery forest of the Brasília National Park, Brazil. Four wood species, important to the Brazilian national genetic conservation programme, were chosen: Astronium fraxinifolium Schott, (Anacardiaceae); Cariniana estrellensis (Raddi) Kuntze, (Lecythidaceae); Didymopanax morototoni (Aublet) Decne. & Planch., (Araliaceae) and Hymenaea courbaril var. stilbocarpa (Hayne) Lee & Lang., (Leguminosae-Caesalpinioideae). While distribution was uneven, no relationship with gradient or togographic position could be detected, but aggregation was perceived. Stocking levels were typically low, from 0.15 to 3.31 individuals ha−1.  相似文献   
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