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Aspergillosis is a significant cause of mortality in captive penguins (Sphenisciformes). An indirect ELISA for the detection of Aspergillus fumigatus-specific immunoglobulin in penguins was developed and standardised by making use of a family-specific antiserum (anti-Aptenodyptes patagonica patagonicus). The results were calculated quantitatively as ELISA units, derived by polynomial regression analysis, and semi-quantitatively as end titres. Serum samples from 61 captive penguins were tested with the assay, and the results were compared with those obtained by counterimmunoelectrophoresis (CIE). The ELISA results correlated with the CIE results only when end titres were reported (R(s) = -0.676, P < 0.002). Fifty-seven of the penguins (93 per cent) were seropositive, but the detection of immunoglobulin did not correlate with clinical disease. At Whipsnade Wild Animal Park, Humboldt's penguins (Spheniscus humboldti) demonstrated higher seropositivity than king penguins (Aptenodyptes patagonicapatagonicus) (P = 0.022), but Humboldt's penguins at Fota Wildlife Park had a significantly higher seropositivity than Humboldt's penguins at Whipsnade (P = 0.035). 相似文献
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Edwards JF 《Veterinary pathology》2002,39(6):744-746
Three cases of bovine, ovarian epidermoid cysts were diagnosed as unilateral benign lesions in three out of 1,971 cows at slaughter. A stratified, cornifying, and squamous epithelium without associated skin adnexal structures or tissues from other embryonic tissue layers lined the cysts. The cysts were small, usually multiple, and contained keratin debris. Macroscopically, they resembled abscesses. These are benign, congenital lesions that are a separate entity from dermoids and teratomas, and they appear to have a higher incidence in cattle with Bos indicus breeding. 相似文献
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Survival and inactivation of classical swine fever virus 总被引:2,自引:0,他引:2
Edwards S 《Veterinary microbiology》2000,73(2-3):175-181
Classical swine fever virus, like many enveloped viruses, may be regarded as moderately fragile. It shows a short but variable survival time in the environment, depending on physical conditions, but may remain viable for prolonged periods in favourable circumstances as found, for example, in stored meat. Published information is reviewed on the survival time of the virus under a variety of physical and chemical conditions, including the variable influence of environmental factors. The principles of virus inactivation by heat, pH or chemicals are discussed, with a consideration of the principles of containment for laboratories working with the virus. 相似文献
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The western corn rootworm, Diabrotica virgifera virgifera LeConte, is the most important pest of maize in the Midwestern USA, and since its detection in 1992 in Europe, also in several countries of Central and Eastern Europe. Methods for managing this pest vary from region to region, depending upon: the different cultural practices in maize production, climatic conditions, soil types, pest behaviour, infestation level and socio-economic conditions. Factors including date of planting, amount of rotated or continuous maize, irrigation, date of rootworm hatching and differences in behaviour of the pest, necessitate developing different management strategies in different areas of the infested countries. Pest monitoring, damage evaluation, threshold development, use of insecticides, risk estimation and crop rotation are some of the starting points in the development of management strategies. Approaches and experiences in D. v. virgifera control and management in Italy, Hungary and the USA, based on the understanding of local agro-ecosystems and socio-economic conditions, are reported. 相似文献
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Abstract. The mean extractable sulphur (S) concentration in 315 upland topsoil samples collected in 1988/89 from beneath pasture in NE Scotland was 13 μg S g−1 (range 2–77 μg S g−1 ). More than two thirds of the samples had S concentrations less than that acceptable for productive soils. Continued decreases in atmospheric S inputs may have increased this proportion subsequently. The analysis of herbage S also indicated that two-thirds of the samples were below 0.2% S. A 'respirometric index', namely CO2 produced during cellulose decomposition without added S as a percentage of that produced with added S, was significantly less than 100% in a quarter of the soils. Results of three different extraction procedures suggested that sulphate in the soils was present mainly as free plus adsorbed rather than precipitated forms. Soil extraction identified a significant non-sulphate S fraction, presumably organic S. The variability in extractable S stemmed from a combination of geographical, depositional and local site and soil factors. Extractable S was significantly correlated with soil organic matter content and inversely with soil pH and together these factors explained 37% of the variability. While significant differences in mean concentrations between geographical area, soil association and drainage status were evident, no trends could be observed between the major soil subgroups or with altitude. 相似文献