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121.
 The influence of compaction on Diplocardia ornata (Smith) burrowing and casting activities, soil aggregation, and nutrient changes in a forest soil were investigated using pot microcosms. Treatments included two levels each of compaction, organic matter, and earthworms. Both burrowing and casting activities were more abundant in uncompacted soil than in compacted soil. Bulk density decreased in microcosms of compacted soil containing D. ornata from 1.76 g cm–3 to 1.49 g cm–3 over the study period. The overall percent of aggregates in the same size classes in compacted soil was less than the percent of aggregates in uncompacted soil. The mean percent of aggregates in earthworm casts for size classes 0.25–1.00 mm was higher for compacted soil than for uncompacted soil. The reverse was true for aggregates in class sizes 2.00–4.00 mm. Soil compaction also affected soil microbial biomass carbon and soil inorganic N concentrations. These results indicate that the burrowing and casting activities of earthworms in compacted forest soils, as in soils of agricultural and pastured lands, can help ameliorate disturbed soils by improving aggregation, reducing bulk density, and increasing nutrient availability. Received: 1 September 1999  相似文献   
122.
This study examined the use of corn gluten feed (CGF) and cottonseed meal (CSM) to partially replace soybean meal and corn in diets for pond‐raised hybrid catfish, Ictalurus punctatus×I. furcatus. Five 28% protein diets containing various combinations of CGF and CSM were evaluated. Fingerling hybrid catfish (mean initial weight: 45 g/fish) were stocked into 25, 0.04‐ha ponds at a rate of 14,826 fish/ha. Fish were fed once daily to apparent satiation for a 179‐d growing season. CGF at 30% of the diet (10% CSM) and a combination of CGF and CSM up to 25% each did not affect physical quality (percentages floatability and feed dust) of the diet. No significant differences were observed in total amount of diet fed, net yield, diet consumption, weight gain, feed conversion ratio (FCR), survival, and fillet proximate nutrient composition among fish fed various diets. Results show that hybrid catfish can utilize CGF at levels up to 30% of the diet (10% CSM), and a combination of CGF and CSM up to 25% each without significantly affecting growth, diet consumption, FCR, and fillet proximate nutrient composition. However, a combination of CGF and CSM at 20% each and above reduced carcass yield.  相似文献   
123.
Motz  Erin C.  Cey  Edwin  Ryan  M. Cathy  Chu  Angus 《Water, air, and soil pollution》2012,223(2):771-785
The attenuation of Escherichia coli and total coliform from secondary treated wastewater effluent under two “at-grade” effluent distribution systems was evaluated in a sandy silt vadose zone in a cold climate. The two at-grade distribution lines had different designs and hydraulic loading rates. Effluent transport was examined using chloride as a tracer. Coliform fate was evaluated relative to the chloride using a combination of in situ pore water sampling and destructive soil sampling, combined with the observation of a dye tracer along excavation sidewalls. Although bacteria attenuation in the subsoil appeared to decrease during colder, winter temperatures (likely due to decreased viability and decreased predation), the subsoil provided about a four log reduction in E. coli over 90 cm of vertical transport. Horizontal transport of bacteria (up to 1.5 m from the line) was likely aided by flow on top of a microbial biomat observed at the soil surface. Both the subsurface dye patterns and the E. coli sampling suggested less preferential flow occurred below the lower loading rate design. At-grade distribution of secondary treated wastewater appears to be a viable alternative to conventional distribution fields at sites with similar climate and soils.  相似文献   
124.
Forecasting the end-of-year crop yield is critical for agricultural decision-making and inherently difficult. Historically, a panel of commodity specialists known as the Agricultural Statistics Board convene regularly to set estimates based on expert review of a combination of survey data and administrative/auxiliary information. To make this process less subjective and more repeatable, we develop a Bayesian hierarchical model that produces superior yield forecasts/estimates, while quantifying different sources of uncertainty. The proposed hierarchical model naturally combines information from multiple monthly surveys measured on different temporal supports, including a field measurement survey and two farmer interview surveys. The dependence between the monthly updated surveys and the serial dependence of the annual yield are incorporated at different levels of the hierarchy. The effectiveness of our approach is demonstrated through an application from the US Department of Agriculture. Empirical results indicate that the hierarchical model produces superior forecasts to both the panel of experts and the composite estimator developed by Keller and Olkin (Technical Report, National Agricultural Statistics Service, 2002), while providing an accurate measure of uncertainty.  相似文献   
125.
126.
<正>大多数鱼类缺乏生物合成维生素C的能力,而且即使某些鱼类能够合成维生素C,合成的数量也不足以满足自身的新陈代谢,所以在饲料的生产中  相似文献   
127.
OBJECTIVE: To report clinical evaluation of headless compression screws for repair of metacarpal/metatarsal (MC/MT3) condylar fractures in horses. STUDY DESIGN: Retrospective case study. SAMPLE POPULATION: Racing Thoroughbreds (n=16) with nondisplaced lateral condylar fractures of MC/MT 3. METHODS: Medical records (1999-2004) of horses with nondisplaced longitudinal fractures of the lateral condyle of MC/MT3 were reviewed. Pre-operative variables retrieved were: patient age, gender, limb involvement, injury occurrence, fracture length, and width, evidence of palmar comminution and degenerative joint disease, number of pre-injury starts, and pre-injury earnings. Post-operative variables retrieved were: surgical complications, surgical time, number of race starts, and post-operative earnings. RESULTS: MC3 (n=11) and MT3 (5) nondisplaced longitudinal fractures of the lateral condyle were repaired with Acutrak Equine (AE) screws. Left front limb fractures were most common (8) followed by left hind (5) and right front (3). Nine fractures occurred during training and 7 during racing; 4 fractures had palmar comminution. No surgical complications occurred. Of 15 horses that returned to training, 11 (73%) raced 306+/-67 days after injury and had greater mean (+/-SD) post-injury earnings/start ($5290.00+/-$8124.00) than pre-injury ($4971.00+/-$2842.00). Screw removal was not required in any horse. CONCLUSION: The AE screw is a viable option for repair of nondisplaced lateral condylar MC/MT3 fractures in Thoroughbred racehorses. CLINICAL RELEVANCE: Adequate stability of nondisplaced lateral condylar fractures can be achieved with a headless tapered compression screw while avoiding impingement on the collateral ligaments and joint capsule of the fetlock joint.  相似文献   
128.
The prevalence of Cryptosporidium in calves and the test properties of six diagnostic assays (microscopy (ME), an immunofluorescence assay (IFA), two ELISA and two PCR assays) were estimated using Bayesian analysis. In a first Bayesian approach, the test results of the four conventional techniques were used: ME, IFA and two ELISA. This four-test approach estimated that the calf prevalence was 17% (95% Probability Interval (PI): 0.1-0.28) and that the specificity estimates of the IFA and ELISA were high compared to ME. A six-test Bayesian model was developed using the test results of the 4 conventional assays and 2 PCR assays, resulting in a higher calf prevalence estimate (58% with a 95% PI: 0.5-0.66) and in a different test evaluation: the sensitivity estimates of the conventional techniques decreased in the six-test approach, due to the inclusion of two PCR assays with a higher sensitivity compared to the conventional techniques. The specificity estimates of these conventional assays were comparable in the four-test and six-test approach. These results both illustrate the potential and the pitfalls of a Bayesian analysis in estimating prevalence and test characteristics, since posterior estimates are variables depending both on the data at hand and prior information included in the analysis. The need for sensitive diagnostic assays in epidemiological studies is demonstrated, especially for the identification of subclinically infected animals since the PCR assays identify these animals with reduced oocyst excretion, which the conventional techniques fail to identify.  相似文献   
129.
The need of a combination of animal treatment and environmental measures against a natural Giardia infection in calves between the age of 1 and 6 months was emphasized and evaluated. Ten commercial farms with a total of 94 calves suffering from chronic diarrhea, ill thriving and impaired growth, were included in two subsequent studies. The first study indicated that treatment of all animals with fenbendazole at 15 mg/kg during 3 consecutive days combined with environmental measures on the final day of treatment, which were either removal of bedding, thorough cleaning and ammonia 10% disinfection or relocation of the treated animals to a Giardia free environment, resulted in a total reduction in cyst excretion for at least 2 weeks. In the second study, the immunofluorescence assay (Merifluor Cryptosporidium/Giardia; Meridian Diagnostics Inc., Cincinnati, Ohio) was used quantitatively and confirmed that the combination of treatment and environmental measures resulted in a total reduction of cyst excretion during 2 weeks and in a significant (P<0.05; >or=98.0%) reduction of the cyst excretion until at least 4 weeks after treatment. Furthermore, there was a noticeable improvement of the clinical symptoms in all animals towards day 28 after treatment and 4 months after treatment health in all calves was normal.  相似文献   
130.
Sensitive and accurate testing for trace amounts of biotechnology-derived DNA from plant material is the prerequisite for detection of 1% or 0.5% genetically modified ingredients in food products or raw materials thereof. Compared to ELISA detection of expressed proteins, real-time PCR (RT-PCR) amplification has easier sample preparation and detection limits are lower. Of the different methods of DNA preparation CTAB method with high flexibility in starting material and generation of sufficient DNA with relevant quality was chosen. Previous RT-PCR data generated with the SYBR green detection method showed that the method is highly sensitive to sample matrices and genomic DNA content influencing the interpretation of results. Therefore, this paper describes a real-time DNA quantification based on the TaqMan probe method, indicating high accuracy and sensitivity with detection limits of lower than 18 copies per sample applicable and comparable to highly purified plasmid standards as well as complex matrices of genomic DNA samples. The results were evaluated with ValiData for homology of variance, linearity, accuracy of the standard curve, and standard deviation.  相似文献   
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