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81.
Data for incidence of summer mastitis in 14 veterinary practices in Jutland (Denmark) were related to meteorological factors using statistical methods. A correlation and regression analysis showed that summer mastitis occurred earlier in years with high temperature and precipitation in early summer. The annual number of cases markedly increased over the years 1953 to 1980. This increase was described by applying a linear increase with with year to a logarithmic transformation of the annual incidence.Analysis of 10-day incidences from the veterinary practices and daily incidence from a test site at St. Vildmose (Jutland) showed that incidence of summer mastitis could be related to temperature and wind velocity within the previous 10 days. High temperature and low wind velocity in this period increase the incidence. This indicates that the incubation period of summer mastitis is at maximum about 10 days. The activity of the fly (Hydrotaea irritans Fall), which acts as mechanical vector for the bacteria is influenced by temperature and wind velocity. This may explain the variation in incidence of summer mastitis during the season and from year to year. 相似文献
82.
A 5-yr study was conducted beginning in 1983 with 460 cows to evaluate the effects of three breeding seasons (30, 45, and 70 d in length) and two times of spring calving, March (early) and April (late), on cattle production under Nebraska Sandhills range conditions. Criteria evaluated included pregnancy and weaning percentages, calving date and distribution, cow weights and body condition at four intervals, calf birth and weaning weights, and cow productivity. The 30-d breeding season included a 10-d estrus synchronization and AI period; in the other breeding seasons only natural breeding was used. The same sires were used over the entire study period. Percentage of cows pregnant and percentage of calves weaned were lower (P less than .01) for cows bred for 30 d than for cows bred for 45 or 70 d. Average calving dates were similar among the breeding groups within the early and late calving herds. Pregnancy rates from AI were higher (P less than .01) for the cows calving in April (64%) than for the cows calving in March (41%). Cows calving in April lost less weight between precalving and prebreeding and were heavier (P less than .05) at prebreeding time than the cows calving in March. Calf weaning weights were not different (P greater than .10) among any of the breeding season groups or between the two calving herds when calves were weaned at a similar age. Cow productivity (calf weaning weight per breeding female) was highest (P less than .05) for the cows bred for 70 d (186 kg), intermediate for the cows bred for 45 d (172 kg), and lowest for cows bred for 30 d (162 kg).(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
83.
The seal death in Danish waters 1988. 2. Virological studies. 总被引:2,自引:0,他引:2
Mass abortions and high mortality were observed in harbour seals in Danish waters during 1988. Severe pneumonia and emphysema were typical clinical and post-mortem findings. Virological studies were carried out to identify the cause of the epidemic. Although seal herpesvirus (SeHV) was isolated in 23 of 114 animals this virus was subsequently found not to be the primary cause of the disease. Following the observation of seroconversion against canine distemper virus (CDV) in diseased seals (Osterhaus & Vedder 1988) a CDV-like morbillivirus (phocine distemper virus, PDV) was identified in organs of diseased animals. It is concluded that the epidemic was caused by introduction of PDV into a highly susceptible population presumably free from morbillivirus infection. The origin of PDV remains unknown but evidence of prior morbillivirus infection has been found in arctic and antarctic seal populations. 相似文献
84.
Ecosystem health as a clinical rotation for senior students in Canadian veterinary schools. 总被引:1,自引:1,他引:0
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C Ribble B Hunter N Larivire D Blanger G Wobeser P Y Daoust T Leighton D Waltner-Toews J Davidson E Spangler O Nielsen 《The Canadian veterinary journal. La revue veterinaire canadienne》1997,38(8):485-490
We describe 4 years of an experimental rotation in ecosystem health offered to senior veterinary students in Canada. Faculty from the 4 Canadian veterinary colleges collaborated in offering the rotation once annually at 1 of the colleges. The 1st rotation was held in Guelph in 1993, followed in successive years by rotations at Saskatoon, Saint-Hyacinthe, and Charlottetown. The rotation is a predominantly field-based experience that allows students to work with veterinary and other role models who are actively engaged in clinical research related to ecosystem health. Five specific field studies that worked particularly well during the rotations are presented. These studies involved investigating mortality in wildlife due to botulism, designing an environmental surveillance system around herds of beef cattle, using belugas to evaluate the health of the St. Lawrence River, dealing with competition for water use by aquaculture and agriculture, and exploring the role of veterinarians during major coastal oil spills. The experience has resulted in our developing the subject matter, field examples, teaching approach, and confidence necessary to make ecosystem health the focus of a productive clinical rotation for senior year veterinary students. 相似文献
85.
S. S. Nielsen 《Zoonoses and public health》2002,49(8):384-387
Milk samples from 120 cows were tested up to 10 times in an enzyme‐linked immunosorbent assay (ELISA) for detection of antibodies to Mycobacterium avium ssp. paratuberculosis. The purpose of the study was to estimate variance components of the assay attributable to laboratory factors using mixed model theory. Because of significant interaction between the between‐run, between‐day and between‐plate variables, the ELISA‐plate variable was nested in run‐number and run‐number was nested in day‐number. The nested variable accounted for 68% of the laboratory variability (P<0.001), whereas the intra‐plate variability accounted for only 0.04% of the laboratory variability (P>0.9). Therefore, it was concluded that the intra‐plate variability could be ignored whereas the variability from the combined run‐day‐plate variable should be considered in any analyses based on the ELISA. 相似文献
86.
Ethylenediamine tetraacetic acid disodium salt (EDTA) was found to be an important addition to anion exchange buffers in terms of the profile and amounts of eluted bovine serum proteins. Hydrogen ion concentration and buffer composition were important when different types of anion exchanger (DEAE 52 cellulose and QAE Sephadex A 50) were used for separation of bovine serum proteins as functional groups of the different anion exchangers did not behave similarly and were therefore not interchangeable. These findings applied to the purification of bovine serum IgG1 amounting to 15 to 25% of the total IgG1 was accomplished using QAE Sephadex A 50 anion exchange chromatography. This was followed by absorption of the IgG1 fraction with Staphylococcus aureus containing protein A to remove minor IgG2 contaminants and gel filtration to exclude traces of the third component of complement (C3). 相似文献
87.
Three hundred sixty-five S1 and 234 S2 seedling populations of timothy (Phleum pratense L.) were examined for their segregations for chlorophyll deficiencies. Arrays of both self-and open-pollination families indicated a lack of concentration at definite levels, with ratios expressed forming a continuous series from 4.6:1 to 572:1 for green: chlorophyll-deficient types in the S1 generation. The S2 populations examined ranged from 1.8:1 to 244:1. Arrays were very similar in their distributions.Sixty-three plants yielded chlorophyll-deficient types in both S1 and S0 populations. From these, it was calculated that 7 to 8 percent of natural self-pollination may occur in timothy.Except at two levels (28:1 and 143:1 tetradisomic) assumption of tetradisomic or simpler inheritance patterns resulted in lower calculated percentages of natural self-pollination than resulted from assumed hexasomic inheritance. No apparent explanation is available to account for these divergences from the regular pattern encountered.Results of cooperative work of the Crops Research Division, Agriculture Research Service, U.S. Department of Agriculture and the Wisconsin Agricultural Experiment Station, Madison.Research Agronomist, Crops Research Division, Agriculture Research Service, U.S. Department of Agriculture and Associate Professor, and Professor of Agronomy, Wisconsin Agricultural Experiment Station, respectively. 相似文献
88.
Ole Wendroth Peter Jürschik K. Christian Kersebaum Hannes Reuter Chris van Kessel Donald R. Nielsen 《Soil & Tillage Research》2001,58(3-4):113-127
To evaluate the quality of the ecosystem and for making resources and land management decisions landscapes have to be assessed quantitatively. For a better understanding of landscape processes and their characterization, the analysis of the inherent variability is a major factor. Four case studies in which problems associated with landscape analysis are discussed. Spatial processes remain a main focus, as their analysis provides information on the relation between relevant state variables in agricultural landscapes. Variogram analysis showed that mineral soil nitrogen (Nmin) sampled in a field at different scales, domains, and times is an instationary spatial process. Spatial association of grain yield, soil index and remotely sensed vegetation index may not be identifiable from kriged contour maps as local coincidence may be obscured behind classified areas. Crop yield in subsequent years and remotely sensed information are not related if a unique response is assumed. An alternative data stratification procedure is described here for the identification of different response functions in agricultural ecosystems. Processes of crop yield and underlying variables are described in autoregressive state-space models. This technique incorporates both deterministic and stochastic relations between different variables and is based on relative changes in space. 相似文献
89.
Young JF Rosenvold K Stagsted J Nielsen JH Andersen HJ 《Journal of agricultural and food chemistry》2005,53(3):745-749
The present study investigates the combined effects of feed-induced increase in polyunsaturated fatty acids (PUFA) content and/or alpha-tocopherol content in pig muscles and preslaughter stress on cell integrity. Cell integrity was determined by plasma lactate dehydrogenase (LDH) activity, and antioxidative status of muscle was measured by activities of the antioxidative enzymes catalase, superoxide dismutase, and glutathione peroxidase. Preslaughter stress increased LDH activity, reflecting loss in cell membrane integrity independent of increased content of PUFA and/or alpha-tocopherol. However, feed-induced increase of PUFA decreased the LDH activity in plasma immediately after slaughter. Catalase activity in the muscle tissue increased as a consequence of the high-PUFA diet, which may indicate an increased demand caused by introduction of oxidative labile PUFA. 相似文献
90.
Joseph A. Kozak Robert M. Aiken Gerald N. Flerchinger David C. Nielsen Liwang Ma Lajpat Ahuja 《Soil & Tillage Research》2007,95(1-2):84-96
RZ-SHAW is a hybrid model, comprised of modules from the Simultaneous Heat and Water (SHAW) model integrated into the Root Zone Water Quality Model (RZWQM) that allows more detailed simulation of different residue types and architectures that affect heat and water transfer at the soil surface. RZ-SHAW allows different methods of surface energy flux evaluation to be used: (1) the SHAW module, where evapotranspiration (ET) and soil heat flux are computed in concert with a detailed surface energy balance; (2) the Shuttleworth–Wallace (S–W) module for ET in which soil surface temperature is assumed equal air temperature; and (3) the PENFLUX module, which uses a Penman transformation for a soil slab under incomplete residue cover. The objective of this study was to compare the predictive accuracy of the three RZ-SHAW modules to simulate effects of residue architecture on net radiation, soil temperature, and water dynamics near the soil surface. The model was tested in Akron, Colorado in a wheat residue-covered (both standing and flat) no-till (NT) plot, and a reduced till (RT) plot where wheat residue was incorporated into the soil. Temperature difference between the soil surface and ambient air frequently exceeded 17 °C under RT and NT conditions, invalidating the isothermal assumption employed in the S–W module. The S–W module overestimated net radiation (Rn) by an average of 69 Wm−2 and underestimated the 3-cm soil temperature (Ts3) by 2.7 °C for the RT plot, attributed to consequences of the isothermal assumption. Both SHAW and PENFLUX modules overestimated midday Ts3 for RT conditions but underestimated Ts3 for NT conditions. Better performances of the SHAW and PENFLUX surface energy evaluations are to be expected as both approaches are more detailed and consider a more discretized domain than the S–W module. PENFLUX simulated net radiation slightly better than the SHAW module for both plots, while Ts3 was simulated the best by SHAW, with a mean bias error of +0.1 °C for NT and +2.7 °C for RT. Simulation results for soil water content in the surface 30 cm (θv30) were mixed. The NT conditions were simulated best by SHAW, with mean bias error for θv30 within 0.006 m3 m−3; RT conditions were simulated best by the PENFLUX module, which was within 0.010 m3 m−3. 相似文献