Genome sequencing analysis of Brazilian chicken anemia virus isolates that lack MSB-1 cell culture tropism     

Nogueira EO  J Piantino Ferreira A  Martins Soares R  Luiz Durigon E  Lazzarin S  Brentano L 《Comparative immunology, microbiology and infectious diseases》2007,30(2):81-96

Specific amino acid (aa) substitutions in VP1, VP2 and VP3 genes were reported as a distinctive feature of the American CIA-1 strain, characterized as having a variable rate of growth and tropism for different MSB-1 cell sublines [Renshaw RW, Soiné C, Weinkle T, O'Connell PH, Ohashi K, Watson S, et al. A hypervariable region in VP1 of chicken anemia virus mediates rate of spread and cell tropism in tissue culture. J Virol 1996;70(12):8872-8]. DNA sequencing of 878 nucleotides from twelve Brazilian CAV, eight of which tested for in vitro isolation in three different sources of MDCC-MSB1 cell line and identified as lacking capacity to propagate in any of these cells, were compared to sequence data available for CAV strains propagated or not in cell culture. Alignment of the deduced aa resulted in a lack of singled out amino acid substitutions in the partial genomic sequences of Brazilian isolates that would entirely contrast them to viruses propagated in MSB-1 cells, indicating that the combined VP1, VP2 and VP3 substitutions observed may not entirely account as sole determinants of CAV isolation and propagation in MDCC-MSB-1 cells.  相似文献   

Cloning and expression of chicken anemia virus VP3 protein in Escherichia coli     

Nogueira-Dantas EO  Ferreira AJ  Astolfi-Ferreira CS  Brentano L 《Comparative immunology, microbiology and infectious diseases》2007,30(3):133-142

Purification of chicken anemia virus (CAV) VP3 protein, expressed in a prokaryotic expression system as histidine-tagged fusion protein is demonstrated in the present study. CAV particle was obtained from infected liver of chicken and DNA was extracted. The VP3 protein gene was amplified from the extracted DNA by polymerase chain reaction (PCR) and cloned. The recombinant expression construct (pTrc-VP3) was identified by PCR and sequencing analysis. Expression of VP3 protein with a molecular mass of approximately 21kDa was confirmed by Western blotting analysis with CAV-specific antibodies. The in vitro expressed VP3 protein was purified to near homogeneity by elution from the gel, as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. The purified VP3 protein was recognized by CAV antibodies in a Western blotting assay. This finding indicates that recombinant VP3 expressed in the pTrcHis2 vector system can be used as antigen to detect anti-CAV antibodies.  相似文献   

1H‐NMR metabolomic profiling of the crayfish Astacus leptodactylus subjected to polyphenol‐enriched diets          下载免费PDF全文

Susan Costantini  Lucia Parrillo  Eliana Guerriero  Dominique Melck  Giovanni Colonna  Maria Grazia Volpe  Marina Paolucci 《Aquaculture Nutrition》2018,24(1):524-538

¹H‐NMR analysis of the hepatopancreas, muscle and haemolymph of Astacus leptodactylus after feeding with polyphenol‐enriched diet is reported. ¹H‐NMR spectra of lipophilic extracts showed the presence of cholesterol, fatty acid residues, phospholipids and triglycerides. ¹H‐NMR spectra of aqueous extracts identified 35 metabolites in the hepatopancreas, 31 in the muscle and 22 in the haemolymph. A total of 20 metabolites (amino acids and their derivatives) were present in the hepatopancreas, the muscle and the haemolymph. A total of 10 metabolites were present in both the hepatopancreas and the muscle (five amino acids, 2‐hydroxybutyrate, choline, myo‐inositol, glycogen and uracil). 2‐Hydroxyisobutyrate and creatine were present in both the hepatopancreas and the haemolymph. Phosphorylethanolamine, phosphocholine and fumarate were present only in the hepatopancreas and isoleucine only in the muscle. Statistical analysis showed that the percentage of weight gain was statistically higher in polyphenol‐enriched diet groups compared to the control and that polyphenols had a stimulating effect on the general metabolism. No stress‐related metabolites were higher in crayfish fed with polyphenol‐enriched diet. Conversely, phosphatidylcholine, cholesterol and DHA, linked to resistance to environmental stress and diseases, were higher compared to the control diet. This study indicates that ¹H‐NMR is a useful tool to study the metabolomics in relation to diet differences.  相似文献   

Influence of the Processing Parameters on the Characteristics of Spherical Bacterial Cellulose     

Ricardo Brandes  Letícia de Souza  Daniel V. F. Vanin  Claudimir A. Carminatti  Eliana M. Oliveira  Regina V. Antônio  Derce O. S. Recouvreux 《Fibers and Polymers》2018,19(2):297-306

Bacterial Cellulose (BC) biosynthesized under agitated conditions can produce hydrogels with spherical shapes and the processing parameters govern this formation of BC hydrogels. The variations of these parameters cause different results in the formation of spherical BC and it is relevant to evaluate these effects. Carbon source, inoculum concentration, volume of the culture medium, stirring speed and incubation temperature were the processing parameters evaluated in this work. Therefore, the results obtained were characterized in terms of dry mass production, water uptake and sphericity of the spherical BC. To do so, Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) were employed as tools for morphological and chemical evaluations. Among the parameters analyzed, the carbon source had the greatest effect on the production of dry mass and water uptake. Glycerol had the greatest growing rate, generating a higher dry mass production with spherical and homogeneous bodies with excellent water absorption indexes. Furthermore, the morphological analysis of BC revealed a typical structure, with high crystallinity index and the presence of typical functional groups. These results indicate a path for the optimized and controlled production of spherical BC, allowing it to be used in many applications, such as enzyme immobilization, water treatment, bone regeneration, spherical cancer model, as adsorption agent and drug delivery.  相似文献   

Study of Analytical Techniques to Determine Chlorpyrifos in the Surface Waterways of the Rural Zone of Ouro Branco,Brazil: A Case Study     

Taciana Maria da Silveira  Renata Domingos Alves  Maria Eliana Lopes Ribeiro de Queiroz  Antônio Augusto Neves  Maurício Xavier Coutrim  Robson José de Cássia Franco Afonso  Gilmare Antônia da Silva 《Water, air, and soil pollution》2016,227(9):335

  相似文献   

Performance of common buckwheat (Fagopyrum esculentum M.) in response to row spacing under ridge and furrow cropping systems in a semiarid region of China     

Ying Jiang  Xinxin Wang  Zhaohai Zeng  Jinjing Han  Michela Schiavon  Chao Tang 《Archives of Agronomy and Soil Science》2018,64(13):1807-1817

Ridge and furrow systems as well as the wide-narrow row spacing are recognized as good management practices in crop production. To obtain some available information concerning the suitable agricultural practices for buckwheat cultivation in arid and semi-arid area, a two-year field study was conducted to investigate the performance of common buckwheat grown under six cropping patterns: conventional flat single/double row planting (NS/ND); furrow single/double row planting (FS/FD); ridge single/double row planting (RS/RD); single row planting with equal row spacing and double row planting with wide-narrow row spacing practice. Plants grown under the FD system exhibited higher water use efficiency (WUE) and rainfall use efficiency (RUE) than plants cultivated with the NS system, increases with 13.7% and 21.9%, respectively, in 2014, while 9.8% and 14.0%, respectively, in 2015. Plants of the FD system also displayed the greatest leaf area index and canopy openness at growth stage of 40, 60, and 80 days after sowing, maximum biomass production (13.96 t ha^?1) and grain yield (3486.2 kg ha^?1) in 2014, and the least pronounced soil nitrate consumption as compared to plants from other tillage systems. We conclude that FD is the optimum planting pattern for common buckwheat cultivation in semi-arid and arid environments.  相似文献   

Non-thermal Plasma as an Innovative Option for the Abatement of Volatile Organic Compounds: a Review     

Marco Schiavon  Vincenzo Torretta  Andrea Casazza  Marco Ragazzi 《Water, air, and soil pollution》2017,228(10):388

Volatile organic compounds (VOCs) cause global and local impacts, resulting in environmental, health, and economic adverse effects. Industrial and waste management activities are the main anthropogenic stationary sources of VOCs in the atmosphere. The traditional technologies for the treatment of VOC-contaminated air present several limitations when treating effluents with low VOC concentrations, high airflow rate, and with compounds with low solubility in water. However, a novel technology, based on non-thermal plasmas (NTPs) and catalysis, has shown promising results in air purification. In this framework, after an initial overview on NTP-catalysis principles, this review presents and discusses 20 recent papers, with a threefold purpose: evaluating the most recent applications of NTP and NTP-catalysis reactors to the treatment of air-VOC mixtures, analyzing all the parameters that may influence the abatement efficiency and the by-product formation, and providing the reader with insights into the choice of the preferable configurations to use, based on the effluent type and the destination of the treated air. As a result of this review, NTPs may represent a promising option for indoor air treatment, especially because of the lower expected byproduct formation when treating low-concentrated VOC mixtures with relatively low air flow rates. If the target is the abatement of higher VOC concentrations, the higher energy efficiency obtainable in such conditions makes NTP-catalysis a cost-effective option for industrial applications. In addition, the formation of simpler and more soluble by-products makes NTPs a suitable technology for air pretreatment upstream of water-based removal technologies, such as absorption columns and biofilters.  相似文献   

Phytodecontamination of the Endocrine Disruptor 4-Nonylphenol in Water Also in the Presence of Two Natural Organic Fractions     

C. Eliana Gattullo  Andreina Traversa  Nicola Senesi  Elisabetta Loffredo 《Water, air, and soil pollution》2012,223(9):6035-6044

The objective of this study was to assess the removal of the endocrine disruptor 4-nonylphenol (NP) at a concentration of 1?mg?L^?1 by ryegrass and radish during germination and growth. The decontamination process was evaluated in water only or in water containing two organic fractions, a soil humic acid (HA) and a river natural organic matter (NOM) at concentrations of 10 and 200?mg?L^?1. The addition of these fractions aimed to simulate the organic content of real aqueous systems. At the end of germination and growth, residual NP was measured by chromatographic analysis. Also, NP phytotoxicity was evaluated during germination. In germination experiments, NP in water only was not toxic for ryegrass and radish which removed, respectively, 37 and 51?% of the initial NP added. In water added with HA or NOM at both doses, in general, NP did not influence or inhibited seed germination. Both doses of HA in water promoted the removal of NP by germinating seeds, whereas NOM exerted differentiated results in the two species as a function of the dose applied. After 2?days of growth, in all treatments both species almost completely removed NP and accumulated a very little fraction of product. This study demonstrated that both ryegrass and radish possess a relevant capacity to remove the endocrine disruptor NP from water also in the presence of different organic fractions, thus suggesting their use in the decontamination of real aquatic systems.  相似文献   

Phytochemical Profile,Antioxidant and Cytotoxic Activities of the Carob Tree (<Emphasis Type="Italic">Ceratonia siliqua</Emphasis> L.) Germ Flour Extracts     

Custódio L  Escapa AL  Fernandes E  Fajardo A  Aligué R  Alberício F  Neng N  Nogueira JM  Romano A 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(1):78-84

This work aimed to evaluate the phytochemical content and to determine the antioxidant and cytotoxic activities of methanol extracts of the carob tree (Ceratonia siliqua L.) germ flour. The extracts were rich in phenolic compounds, had considerable antioxidant activity, and reduced the viability of cervical (HeLa) cancer cells. The chemical content and the biological activities of the extracts were significantly affected by gender and cultivar. Female cultivar Galhosa had the highest levels of phenolic compounds, and the highest antioxidant activity. Extracts from the hermaphrodite trees and from the female cultivars Galhosa and Costela/Canela exhibited the highest cytotoxic activity. The most abundant compound was theophylline. The phenolic content was correlated to both antioxidant and cytotoxic activities. Our findings provide new knowledge about the health implications of consuming food supplemented with carob germ flour.  相似文献   

Serological response of guinea pigs to oily and aqueous inactivated vaccines containing a Brazilian isolate of the Bovine Viral Diarrhea Virus (BVDV)     

Jordão RS  Ribeiro CP  Pituco EM  Okuda LH  Del Fava C  Stefano Ed  Filho MM  Mehnert DU 《Research in veterinary science》2011,91(2):311-315

Bovine Viral Diarrhea Virus (BVDV) is widespread in cattle in Brazil and research shows its large antigenic variability. Available vaccines are produced with virus strains isolated in other countries and may not be effective. In this study, inactivated vaccines containing the Brazilian BVDV-Ib IBSP11 isolate were developed and tested on 6 groups of 10 guinea pigs (Cavia porcellus). Animals in groups A and C received an aqueous vaccine (aluminum hydroxide); B and D groups received an oily vaccine (Montanide ISA50); Group E positive-control animals were given an imported commercial vaccine with BVDV-Ia Singer; Group F animals were sham vaccinated (negative control). Groups A, B and E received two doses, and Groups C and D, three, every 21 days. Twelve blood samples were taken, at 21-day intervals over 231 days, and evaluated for antibody titer through virus-neutralization (VN), using a homologous strain (IBSP11), and a heterologous strain (BVDV-Ia NADL). Most animals, 42 days following the first dose, seroconverted to both strains and, after the second dose, there was a significant increase of titers in all groups. The oily formulation induced greater response after the third administration. This increase was not observed with the aqueous vaccines, regardless of the virus used in the VN. Antibody decline was more rapid in animals that received aqueous vaccines. The results showed the importance of studying the influence of endemic strains of commercial vaccines, to improve the efficacy of BVD vaccination. Use of the endemic strain in vaccine formulation presented promising results, as well as the use of guinea pigs as a laboratory model.  相似文献