Efficacy of an oral live vaccine for veterinary use against pseudotuberculosis     

Benoît Quintard  Thierry Petit  Nathalie Ruvoen  Elisabeth Carniel  Christian E. Demeure   《Comparative immunology, microbiology and infectious diseases》2010,33(6):e59-e65

Pseudotuberculosis, an infection caused by the ubiquitous enteropathogenic bacterium Yersinia pseudotuberculosis, is a recurrent veterinary problem in livestock and zoo animals. The only vaccine currently available in zoos is Pseudovac (a mixture of killed strains of various serotypes), but its efficacy is not well established. We show here that Pseudovac does not protect guinea pigs against a severe Y. pseudotuberculosis infection. We thus evaluated the possibility of using a live attenuated Y. pseudotuberculosis strain (IP32680) as an oral vaccine against animal pseudotuberculosis. We report that IP32680 is avirulent for guinea pigs and induces a strong IgG response against various serotypes of Y. pseudotuberculosis. One and two oral inoculations of IP32680 provided 50% and 83% protection, respectively against a severe infection with a highly pathogenic strain. The avirulent Y. pseudotuberculosis IP32680 is therefore much more protective than Pseudovac and may represent a valuable oral vaccine against pseudotuberculosis in zoo animals.  相似文献   

Progesterone intoxication inducing marked sedation in a cat     

Marc P. Dhumeaux  Elisabeth C.R. Snead  Germaine C. Hung  Susan M. Taylor 《Journal of Feline Medicine and Surgery》2010,12(10):811-813

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Protocol: using virus-induced gene silencing to study the arbuscular mycorrhizal symbiosis in <Emphasis Type="Italic">Pisum sativum</Emphasis>     

Mette Grønlund  Anne Olsen  Elisabeth I Johansen  Iver Jakobsen 《Plant methods》2010,6(1):28

Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants, which are difficult to transform. The pea early-browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. However, the available PEBV-VIGS protocols are inadequate for studying genes involved in the symbiosis with arbuscular mycorrhizal fungi (AMF).  相似文献   

Investigations of barley stripe mosaic virus as a gene silencing vector in barley roots and in <Emphasis Type="Italic">Brachypodium distachyon</Emphasis> and oat     

Andrzej Pacak  Katrin Geisler  Bodil Jørgensen  Maria Barciszewska-Pacak  Lena Nilsson  Tom Hamborg Nielsen  Elisabeth Johansen  Mette Grønlund  Iver Jakobsen  Merete Albrechtsen 《Plant methods》2010,6(1):26

Background  

Gene silencing vectors based on Barley stripe mosaic virus (BSMV) are used extensively in cereals to study gene function, but nearly all studies have been limited to genes expressed in leaves of barley and wheat. However since many important aspects of plant biology are based on root-expressed genes we wanted to explore the potential of BSMV for silencing genes in root tissues. Furthermore, the newly completed genome sequence of the emerging cereal model species Brachypodium distachyon as well as the increasing amount of EST sequence information available for oat (Avena species) have created a need for tools to study gene function in these species.  相似文献   

Inter-laboratory comparison of four RT-PCR based methods for the generic detection of pospiviroids in tomato leaves and seeds     

Thibaut Olivier  Vaidevutis Šveikauskas  Elisabeth Demonty  Kris De Jonghe  Pascal Gentit  Mojca Viršček-Marn  Sabine Grausgruber-Gröger  Sébastien Morio  Francesco Faggioli  Michèle Visage  Frédéric Fauche  Maria Gusina  Marta Luigi  Helena Lasner  Irena Mavrič-Pleško 《European journal of plant pathology / European Foundation for Plant Pathology》2016,144(3):645-654

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Comparative growth performance of pigs immunised with a gonadotrophin releasing factor vaccine with surgically castrated pigs and entire boars raised under conventionally managed conditions     

Fuchs T  Nathues H  Koehrmann A  Andrews S  Brock F  Klein G  Beilage Eg 《Berliner und Münchener tier?rztliche Wochenschrift》2011,124(1-2):22-27

The objective of this study was to determine the effect of using a gonadotrophin releasing factor (GnRF) vaccine on growth performance of male pigs. Vaccinated pigs were compared with surgically castrated pigs and entire males under conventionally managed farm conditions. Male pigs were randomly assigned to three treatment groups: pigs surgically castrated during the first week of life (group "C", n=274), pigs immunised twice during the fattening period with a GnRF vaccine, the first when 13-14 weeks of age and the second when 20-21 weeks of age (group "V" n=280) and entire males (group "E", n=56). Average daily weight gain (ADG) was measured during the study. Additionally, feed conversion ratio (FCR) as defined by feed intake/weight gain and feed consumption was assessed during the fattening period. No difference in the ADG during the fattening period could be determined between "C" and "V". From the start of the study until slaughter at study day 160/173 the ADG in group "E" was lower (625 g/day; P) than in group "C" (681 g/day; P < 0.001) and group "V" (675 g/day; P < 0.01). The FCR during the fattening period until study day 160, three days before the first batch of pigs were slaughtered at 24-25 weeks of age, was higher in group"C" (2.72 kg feed intake/kg weight gain) compared with group "V" (2.44 kg feed intake/kg weight gain; P < 0.001) and group "E" (2.44 kg feed intake/kg weight gain; P <0.01). Pigs in group "V" performed similarly to those in group "E" until the second vaccination (day 135) after which time they showed a substantial increase in feed intake and ADG, while retaining an FCR advantage over group C.  相似文献   

Glucocorticoid-dependent hypoadrenocorticism with thrombocytopenia and neutropenia mimicking sepsis in a Labrador retriever dog     

Snead E  Vargo C  Myers S 《The Canadian veterinary journal. La revue veterinaire canadienne》2011,52(10):1129-1134

Glucocorticoid-deficient hypoadrenocorticism (GDH) with immune-mediated-neutropenia (IMN) and -thrombocytopenia (IMT) were diagnosed in a 3-year-old Labrador retriever dog. Glucocorticoid-deficient hypoadrenocorticism is rare and diagnostically challenging as clinical signs and laboratory abnormalities are often nonspecific. Immune-mediated cytopenias and other autoimmune disorders, as part of an autoimmune polyglandular syndrome have been reported with hypoadrenocorticism in humans. This is the first reported case of hypoadrenocorticism and bicytopenia in a dog.  相似文献   

Development of an indirect ELISA-NcSRS2 for detection of Neospora caninum antibodies in cattle   总被引：1，自引：0，他引：1  

Borsuk S  Andreotti R  Leite FP  Pinto Lda S  Simionatto S  Hartleben CP  Goetze M  Oshiro LM  Matos Mde F  Berne ME 《Veterinary parasitology》2011,177(1-2):33-38

Neosporosis is of alarming economic concern in the cattle industry. The effectiveness of diagnostic tests for detecting specific antibodies against Neospora caninum is hampered by potential cross-reaction with other coccidia. Use of a single specific antigen might improve test specificity. An indirect enzyme-linked immunosorbent assay (ELISA) was developed using the truncated protein NcSRS2 expressed in Escherichia coli. The ELISA results were compared with those of the indirect fluorescence antibody test (IFAT). Receiver Operating Characteristic (ROC) and Tests in the Absence of a Gold Standard (TAGS) analysis revealed an assay having 96% specificity and 95% sensitivity when applied to 145 positive and 352 negative sera from two distinct cattle populations. Using OD ≤ 0.095 as the cut-off point, the assay's negative and positive predictive values ranged from 98.8% to 50.8% and from 58.8% to 99.1%, respectively, depending on neosporosis prevalence in a given area. The novel ELISA-NcSRS2 format described in the present report constitutes a specific and sensitive method for detecting N. caninum in cattle.  相似文献   

First morphological characterization of 'Candidatus Mycoplasma turicensis' using electron microscopy     

Willi B  Museux K  Novacco M  Schraner EM  Wild P  Groebel K  Ziegler U  Wolf-Jäckel GA  Kessler Y  Geret C  Tasker S  Lutz H  Hofmann-Lehmann R 《Veterinary microbiology》2011,149(3-4):367-373

At least three haemotropic mycoplasmas have been recognized in cats: Mycoplasma haemofelis (Mhf), 'Candidatus Mycoplasma haemominutum' (CMhm) and 'Candidatus M. turicensis' (CMt). The latter was originally identified in a Swiss pet cat with haemolytic anaemia and shown to be prevalent in domestic cats and wild felids worldwide using molecular methods. So far, there has been no confirmatory morphological evidence of the existence of CMt presumably due to low blood loads during infection while CMhm has only been characterized by light microscopy with discrepant results. This study aimed to provide for the first time electron microscopic characteristics of CMt and CMhm and to compare them to Mhf. Blood samples from cats experimentally infected with CMt, CMhm and Mhf were used to determine copy numbers in blood by real-time PCR and for transmission and scanning electron microscopy. High resolution scanning electron microscopy revealed CMt and CMhm to be discoid-shaped organisms of 0.3 μm in diameter attached to red blood cells (RBCs). In transmission electron microscopy of CMt, an oval organism of about 0.25 μm with several intracellular electron dense structures was identified close to the surface of a RBC. CMhm and CMt exhibited similar morphology to Mhf but had a smaller diameter. This is the first study to provide morphological evidence of CMt thereby confirming its status as a distinct haemoplasma species, and to present electron microscopic features of CMhm.  相似文献   

Diverging opinions of supplementation programmes between mothers of small children and staff at primary health clinics in the Western Cape Province of South Africa     

Iversen PO  Høisæther EA  Morseth M  Herselman M 《Public health nutrition》2011,14(5):923-930

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