Fruit and vegetables are similarly categorised by 8-13-year-old children     

Sepulveda KK  Beltran A  Watson K  Baranowski T  Baranowski J  Islam N  Missaghian M 《Public health nutrition》2009,12(2):175-187

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Single dose adenovirus vectored vaccine induces a potent and long-lasting immune response against rabbit hemorrhagic disease virus after parenteral or mucosal administration     

Fernández E  Toledo JR  Chiong M  Parra F  Rodríguez E  Montero C  Méndez L  Capucci L  Farnós O 《Veterinary immunology and immunopathology》2011,142(3-4):179-188

Rabbit hemorrhagic disease virus (RHDV) is the etiological agent of a lethal and contagious disease of rabbits that remains as a serious problem worldwide. As this virus does not replicate in cell culture systems, the capsid protein gene has been expressed in heterologous hosts or inserted in replication-competent viruses in order to obtain non-conventional RHDV vaccines. However, due to technological or safety issues, current RHDV vaccines are still prepared from organs of infected rabbits. In this work, two human type 5 derived replication-defective adenoviruses encoding the rabbit hemorrhagic disease virus VP60 capsid protein were constructed. The recombinant protein was expressed as a multimer in mouse and rabbit cell lines at levels that ranged from approximately 120 to 160 mg/L of culture. Mice intravenously or subcutaneously inoculated with a single 10(8) gene transfer units (GTU) dose of the AdVP60 vector (designed for VP60 intracellular expression) seroconverted at days 7 and 14 post-immunization, respectively. This vector generated a stronger response than that obtained with a second vector (AdVP60sec) designed for VP60 secretion. Rabbits were then immunized by parenteral or mucosal routes with a single 10(9)GTU dose of the AdVP60 and the antibody response was evaluated using a competition ELISA specific for RHDV or RHDVa. Protective hemagglutination inhibition (HI) titers were also promptly detected and IgG antibodies corresponding with inhibition percentages over 85% persisted up to one year in all rabbits, independently of the immunization route employed. These levels were similar to those elicited with inactivated RHDV or with VP60 obtained from yeast or insect cells. IgA specific antibodies were only found in saliva of rabbits immunized by intranasal instillation. The feasibility of VP60 production and vaccination of rabbits with replication-defective adenoviral vectors was demonstrated.  相似文献   

Frequent Exposure of Mice to Crude Brucella abortus Proteins Down‐regulates Immune Response     

A. Oate  E. Andrews  A. Beltran  G. Eller  G. Schurig  H. Folch 《Zoonoses and public health》2000,47(9):677-682

Mice repeatedly immunized via the intraperitoneal route with a Brucella abortus antigen lost their ability to develop a strong in vitro lymphoproliferative response. This result correlates with a decreased tendency of the lymphoid population to produce interferon‐γ when stimulated in culture with the immunizing antigen. With respect to the humoral response, as the number of immunizations increased, the animals produced more specific immunoglobulin M and immunoglobulin G1 antibodies. It is postulated that the long‐term exposure of an animal to Brucella antigen changes the nature of the immune response from a T‐cell‐mediated response to a humoral response favouring the establishment of the disease.  相似文献   

Agronomic characterization of diploid hybrid families derived from crosses between haploids of the common potato and three wild Argentinian tuber-bearing species   总被引：1，自引：0，他引：1  

Matías Santini  Elsa Lucila Camadro  Olga Noemí Marcellán  Luis Ernesto Erazzú 《American Journal of Potato Research》2000,77(4):211-218

Five diploid hybrid families derived from crosses between haploids of three Argentinian cultivars and three diploid wild species,Solanum gourlayi, S. chacoense andS. spegazzinii, were evaluated in a field experiment in Balcarce. All plants were androsterile and, thus, could not be screened for 2n pollen production. Data on agronomic tuber traits were statistically analyzed. Differences among families were non-significant for tuber number/plant, but were significant for tuber weight/plant and dry matter content (with values above 21.0% in two of them). The relative frequencies of tubers in the categories established for size, skin texture, and eye depth were family dependent, but they were familiy independent in the categories established for tuber shape and skin color. Great morphological variability was detected for all the traits studied, and plants with high yield and high dry matter content were identified. These families can be used as sources of germplasm in breeding and to explore heterotic responses in 2x × 4x crosses, given the capacity of some plants to produce 2n eggs, as found in a related investigation.  相似文献   

Computed tomographic findings in three dogs naturally infected with Crenosoma vulpis          下载免费PDF全文

Jeremy R. Mortier  Caroline J. Fina  Elsa Edery  Crystal L. White  Marc P. Dhumeaux 《Veterinary radiology & ultrasound》2018,59(1):27-31

Crenosoma vulpis is a nematode lungworm found in wild and domestic canids in some parts of North America and Europe. Reported radiographic findings are nonspecific and consist of a combination of bronchial and interstitial changes of variable severity. This retrospective, case series study aimed to describe thoracic computed tomographic (CT) findings for a group of dogs with confirmed crenosomosis. Selection criteria were presentation with a chronic cough during the period of January 2016 to February 2017, evaluation by thoracic CT, and final diagnosis of C. vulpis infection based on bronchoscopic findings, bronchoalveolar lavage fluid analysis, and quantitative polymerase chain reaction. Medical records and CT images were retrieved and reviewed by a board‐certified veterinary internist, a veterinary internal medicine resident, two board‐certified veterinary radiologists, and a veterinary radiology intern, and findings were recorded. Three dogs met inclusion criteria. Thoracic CT findings for all dogs included the following: diffuse bronchial wall thickening, multifocal peribronchial ground glass attenuation, consolidation of the pulmonary parenchyma, and cylindrical bronchiectasis. In two dogs, the bronchial wall thickening was irregular to nodular, which was consistent with the bronchoscopic findings. Two dogs showed pulmonary parenchymal bands. Thoracic computed tomographic changes in dogs with C. vulpis are consistent with those seen on thoracic radiographs and crenosomosis should be considered in dogs with these findings.  相似文献   

Clinical signs,imaging findings,and outcome in twelve cats with internal ophthalmoparesis/ophthalmoplegia          下载免费PDF全文

Negar Hamzianpour  Richard Lam  Roser Tetas  Elsa Beltran 《Veterinary ophthalmology》2018,21(4):382-390

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Computed tomographic and magnetic resonance imaging of a coup contrecoup traumatic brain injury in a horse     

R. E. Morgan  B. Dunkel  S. Spiro  E. Beltran 《Equine Veterinary Education》2022,34(4):e151-e156

A 3-year-old Thoroughbred mare found recumbent in the field was referred for further assessment with suspicion of a skull fracture. Neurological examination identified compulsive tight circling to the left, and hypermetria in all four limbs. The mare was obtunded, with a mild head tilt to the right, absent menace response of the right eye and decreased facial sensation on the right. Standing computed tomographic examination revealed a subtle depression fracture of the dorsal calvarium and moderate intra-axial midline shift consistent with a traumatic brain injury (TBI). Despite supportive treatment, the mare deteriorated and was subjected to euthanasia. Post-mortem high field magnetic resonance imaging revealed findings consistent with a small cerebral contusion adjacent to the fracture site, and moderate to severe ipsilateral cerebral oedema within the caudal cerebrum and rostral brainstem, consistent with a coup contrecoup TBI. Brainstem lesions indicate a poor prognosis and support the decision for euthanasia. This is the first report of the imaging findings of a coup contrecoup TBI in a horse.  相似文献   

Clinical Course of Acute Canine Polyradiculoneuritis Following Treatment with Human IV Immunoglobulin     

K Hirschvogel  K Jurina  TA Steinberg  LA Matiasek  K Matiasek  E Beltrán  A Fischer 《Journal of the American Animal Hospital Association》2012,48(5):299-309

Treatment of dogs with acute canine polyradiculoneuritis (ACP) is restricted to physical rehabilitation and supportive care. In humans with Guillain-Barré syndrome, the counterpart of ACP, randomized trials show that IV immunoglobulin (IVIg) speeds recovery. The authors of the current study hypothesized that dogs with ACP would tolerate IVIg well and recover faster than dogs managed with supportive treatment only. Sixteen client-owned dogs with ACP were treated with IVIg, and 14 client-owned dogs served as a retrospective control group. Diagnosis was confirmed using clinical features, electrodiagnostics, cerebrospinal fluid analysis, and muscle/nerve biopsies. The duration of the initial progressive phase, the time from IVIg administration until the dogs were ambulating without assistance, and the duration of the complete episode were evaluated. Adverse reactions (anaphylaxis, mild hematuria) were observed in two dogs. Dogs treated with IVIg were ambulating without assistance after a median of 27.5 days (range, 15-127 days) from onset of clinical signs. The control group was ambulatory without assistance at a median of 75.5 days (range, 5-220 days). Even though this result is not statistically significant, there is a clear trend toward faster recovery in dogs treated with IVIg.  相似文献   

Management strategies of mealybug pests of citrus in mediterranean countries     

José?Carlos?Franco  Pompeo?Suma  Elsa?Borges?da?Silva  Daniel?Blumberg  Zvi?MendelEmail author 《Phytoparasitica》2004,32(5):507-522

Six mealybug species have been reported as citrus pests in the Mediterranean Basin: the citrus mealybugPlanococcus citri (Risso), the citriculus mealybugPseudococcus cryptus Hempel, the longtailed mealybugPseudococcus longispinus (Targioni-Tozzetti), the citrophilus mealybugPseudococcus calceolariae (Maskell), the obscure mealybugPseudococcus viburni (Signoret) and the spherical mealybugNipaecoccus viridis (Newstead). Some of these species,e.g. N. viridis, have recently been introduced into the region and are still spreading. Mealybugs are usually occasional or minor pests of citrus, but some species can reach key pest status. Mealybug management strategies in citrus have been based mostly on classical biological control and, to a lesser extent, on augmentative releases. However, chemical control is widely used, mainly because of the poor adaptation of the principal natural enemies to the climatic conditions of the Mediterranean. The application of pheromones is still restricted to monitoring the citrus mealybug, whose sex pheromone is commercially available. Mass trapping and mating disruption should be considered for possible use in IPM programs as an alternative method to supplementary chemical treatments. Enhancement of biological control through management of ant populations is another promising tactic for control of mealybugs. Strategies for managing mealybug pests of citrus, and possible levels of integration of different tactics according to the pest status, are discussed. http://www.phytoparasitica.org posting Oct. 3, 2004.  相似文献   

Biodistribution of three photosensitizers in dogs with spontaneous tumors     

Gloi AM  Beck E 《Veterinary therapeutics : research in applied veterinary medicine》2003,4(2):155-165

Photodynamic therapy (PDT) has been considered a potential method for tumor eradication. The present study was designed to assess the efficacy of PDT as an alternative treatment approach. Photosensitizers, such as porfimer sodium, tin ethyl etiopurpurin, and aluminum chlorophthalocyanine, were administered i.v. to dogs, and tissue samples were harvested 24 to 300 hours later. The uptake of the photosensitizers in tumor (fibrosarcoma) and adjacent normal tissue biopsies was quantified by tissue solubilization technique and fluorimetry. In addition, the pharmacokinetics and selectivity of the photosensitizers were addressed by two-phase exponential function and specific uptake ratio, respectively. Porfimer sodium exhibited a longer elimination half-life (175.3 hr), slower clearance (0.0028 L/kg/hr), and a larger area under the curve (1075 microg/g/hr) in tumors than did tin ethyl etiopurpurin or aluminum chlorophthalocyanine. As a result, porfimer sodium showed a good selectivity in tumors located in muscle and skin. The study provided clinical information for determination of the efficacy of different PDT alternatives.  相似文献